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DK4144844T3 - Beskyttede dna-templates til genmodificering og øget homolog rekombination i celler og fremgangsmåder til anvendelse deraf - Google Patents

Beskyttede dna-templates til genmodificering og øget homolog rekombination i celler og fremgangsmåder til anvendelse deraf

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Publication number
DK4144844T3
DK4144844T3 DK22189338.1T DK22189338T DK4144844T3 DK 4144844 T3 DK4144844 T3 DK 4144844T3 DK 22189338 T DK22189338 T DK 22189338T DK 4144844 T3 DK4144844 T3 DK 4144844T3
Authority
DK
Denmark
Prior art keywords
cells
methods
homologous recombination
dna templates
gene modification
Prior art date
Application number
DK22189338.1T
Other languages
English (en)
Inventor
Ryan L Frisch
Original Assignee
Dupont Us Holding Llc
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Filing date
Publication date
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Publication of DK4144844T3 publication Critical patent/DK4144844T3/da

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Bioinformatics & Cheminformatics (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK22189338.1T 2015-10-12 2016-10-11 Beskyttede dna-templates til genmodificering og øget homolog rekombination i celler og fremgangsmåder til anvendelse deraf DK4144844T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562240140P 2015-10-12 2015-10-12
EP16791150.2A EP3362560B1 (en) 2015-10-12 2016-10-11 Protected dna templates for gene modification and increased homologous recombination in cells and methods of use

Publications (1)

Publication Number Publication Date
DK4144844T3 true DK4144844T3 (da) 2025-11-24

Family

ID=57233846

Family Applications (2)

Application Number Title Priority Date Filing Date
DK16791150.2T DK3362560T3 (da) 2015-10-12 2016-10-11 Beskyttede dna-templater til genmodificering og forøget homolog rekombination i celler og fremgangsmåder til anvendelse
DK22189338.1T DK4144844T3 (da) 2015-10-12 2016-10-11 Beskyttede dna-templates til genmodificering og øget homolog rekombination i celler og fremgangsmåder til anvendelse deraf

Family Applications Before (1)

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DK16791150.2T DK3362560T3 (da) 2015-10-12 2016-10-11 Beskyttede dna-templater til genmodificering og forøget homolog rekombination i celler og fremgangsmåder til anvendelse

Country Status (9)

Country Link
US (1) US20180273979A1 (da)
EP (2) EP4144844B1 (da)
JP (1) JP7011590B2 (da)
KR (1) KR102628801B1 (da)
AU (1) AU2016338785B2 (da)
CA (1) CA2999050C (da)
DK (2) DK3362560T3 (da)
FI (1) FI4144844T3 (da)
WO (1) WO2017066175A1 (da)

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AU2016338785A1 (en) 2018-04-12
CA2999050A1 (en) 2017-04-20
BR112018007351A2 (pt) 2018-10-23
EP3362560B1 (en) 2022-08-10
WO2017066175A1 (en) 2017-04-20
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AU2016338785B2 (en) 2022-07-14
JP2018530352A (ja) 2018-10-18
FI4144844T3 (fi) 2025-11-24
EP4144844B1 (en) 2025-09-10
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