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SG11201606934SA - Improved reprogramming methods and cell culture platforms - Google Patents

Improved reprogramming methods and cell culture platforms

Info

Publication number
SG11201606934SA
SG11201606934SA SG11201606934SA SG11201606934SA SG11201606934SA SG 11201606934S A SG11201606934S A SG 11201606934SA SG 11201606934S A SG11201606934S A SG 11201606934SA SG 11201606934S A SG11201606934S A SG 11201606934SA SG 11201606934S A SG11201606934S A SG 11201606934SA
Authority
SG
Singapore
Prior art keywords
cell culture
reprogramming methods
culture platforms
improved reprogramming
improved
Prior art date
Application number
SG11201606934SA
Inventor
Bahram Valamehr
Peter Flynn
Ramzey Abujarour
Megan Robinson
Original Assignee
Fate Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fate Therapeutics Inc filed Critical Fate Therapeutics Inc
Publication of SG11201606934SA publication Critical patent/SG11201606934SA/en

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/724—Glycosyltransferases (EC 2.4.)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201606934SA 2014-03-04 2015-03-04 Improved reprogramming methods and cell culture platforms SG11201606934SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461947979P 2014-03-04 2014-03-04
PCT/US2015/018801 WO2015134652A1 (en) 2014-03-04 2015-03-04 Improved reprogramming methods and cell culture platforms

Publications (1)

Publication Number Publication Date
SG11201606934SA true SG11201606934SA (en) 2016-09-29

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SG10201807292YA SG10201807292YA (en) 2014-03-04 2015-03-04 Improved reprogramming methods and cell culture platforms
SG11201606934SA SG11201606934SA (en) 2014-03-04 2015-03-04 Improved reprogramming methods and cell culture platforms

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Country Status (11)

Country Link
US (2) US11268069B2 (en)
EP (4) EP3805369B1 (en)
JP (7) JP6722108B2 (en)
KR (4) KR102460549B1 (en)
CN (3) CN106414721A (en)
AU (3) AU2015227184B2 (en)
CA (1) CA2941004A1 (en)
ES (1) ES2966757T3 (en)
PT (1) PT3114214T (en)
SG (2) SG10201807292YA (en)
WO (1) WO2015134652A1 (en)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200113286A (en) 2010-12-22 2020-10-06 페이트 세러퓨틱스, 인코포레이티드 Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
KR102460549B1 (en) 2014-03-04 2022-10-28 페이트 세러퓨틱스, 인코포레이티드 Improved reprogramming methods and cell culture platforms
US10023879B2 (en) * 2014-06-04 2018-07-17 Fate Therapeutics, Inc. Minimal volume reprogramming of mononuclear cells
CN114058582A (en) 2015-01-26 2022-02-18 菲特治疗公司 Methods and compositions for inducing hematopoietic cell differentiation
CA2993201A1 (en) 2015-07-21 2017-01-26 The Children's Medical Center Corporation Pd-l1 expressing hematopoietic stem cells and uses
KR20250007036A (en) * 2015-10-16 2025-01-13 페이트 세러퓨틱스, 인코포레이티드 Platform for the induction and maintenance of ground state pluripotency
US20190093072A1 (en) 2015-10-21 2019-03-28 Indiana University Research And Technology Corporation Methods of generating human inner ear sensory epithelia and sensory neurons
CN115806940A (en) 2015-11-04 2023-03-17 菲特治疗公司 Genome engineering of pluripotent cells
EP3371301A4 (en) 2015-11-04 2019-06-26 Fate Therapeutics, Inc. METHODS AND COMPOSITIONS FOR INDUCING THE DIFFERENTIATION OF HEMATOPOIETIC CELLS
CA3010764A1 (en) * 2016-01-12 2017-07-20 Lonza Walkersville, Inc. Methods and vectors to produce vector free induced pluripotent stem cells
KR102537361B1 (en) * 2016-12-27 2023-05-26 스미또모 가가꾸 가부시키가이샤 Methods for evaluating and selecting artificial pluripotent stem cells, and methods for producing artificial pluripotent stem cells
CN106754729B (en) * 2016-12-31 2020-03-17 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 Method for preparing induced pluripotent stem cells by using Xist Tale inhibitory transcription factor
WO2019060708A1 (en) 2017-09-22 2019-03-28 The Children's Medical Center Corporation Treatment of type 1 diabetes and autoimmune diseases or disorders
CA3078734A1 (en) * 2017-10-11 2019-04-18 Fate Therapeutics, Inc. Cellular reprogramming using temporal and transient plasmid vector expression system
SG11202005173SA (en) 2017-12-22 2020-06-29 Fate Therapeutics Inc Enhanced immune effector cells and use thereof
KR102791763B1 (en) * 2017-12-28 2025-04-07 더 제이. 데이비드 글래드스톤 인스티튜트, 어 테스터멘터리 트러스트 이스타빌리쉬드 언더 더 윌 오브 제이. 데이비드 글래드스톤 Generation of induced pluripotent cells by CRISPR activation
EP3768827A1 (en) * 2018-03-22 2021-01-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for reprogramming somatic cells
CA3121128A1 (en) 2018-12-02 2020-06-11 Fate Therapeutics, Inc. Immunotherapies using enhanced ipsc derived effector cells
CN109679918A (en) * 2018-12-25 2019-04-26 合肥中科干细胞再生医学有限公司 A kind of preparation method of convenient and fast people's inductive pluripotent stem cells
JP7273141B2 (en) * 2019-03-29 2023-05-12 富士フイルム株式会社 Method for Producing Pluripotent Stem Cells Capable of Differentiating into Specific Cells and Application Thereof
CN110564673A (en) * 2019-09-26 2019-12-13 广东工业大学 Stem cell culture solution and application thereof
CA3150477A1 (en) * 2019-12-11 2021-06-17 Luis Haupt Expansion of stem cells in suspension in a bioreactor
EP4168538A4 (en) 2020-06-19 2024-03-20 Fate Therapeutics, Inc. COMBINATION OF IPSC-DERIVED EFFECTOR CELL TYPES FOR USE IN IMMUNOTHERAPY
AU2021296296A1 (en) * 2020-06-22 2023-01-19 Life Technologies Corporation Methods and compositions for cultivating pluripotent cell suspensions
WO2022072883A1 (en) * 2020-10-02 2022-04-07 Fate Therapeutics, Inc. Improved reprogramming, maintenance and preservation for induced pluripotent stem cells
US20230302044A1 (en) * 2022-03-28 2023-09-28 Steve Reilly Composition to increase cellularlongevity
EP4504799A1 (en) 2022-04-08 2025-02-12 Fate Therapeutics, Inc. Chimeric antigen receptor for tumor targeting
IL316101A (en) 2022-04-08 2024-12-01 Fate Therapeutics Inc Cells having solid tumor targeting backbone and use thereof
KR20250106741A (en) * 2022-10-12 2025-07-10 페킹 유니버시티 Chemical reprogramming methods and pluripotent stem cells
WO2025152036A1 (en) * 2024-01-16 2025-07-24 Chiou Guang Yuh System and method for increasing the transduction capability and reprogramming efficiency in targeting cells and tissues
WO2025208002A1 (en) * 2024-03-28 2025-10-02 Altos Labs, Inc. Targeted expression of regeneration factors in aged/senescent cells
CN118979011A (en) * 2024-10-22 2024-11-19 杭州瑞普晨创科技有限公司 Method for establishing human pluripotent stem cell line

Family Cites Families (153)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5506337A (en) 1985-03-15 1996-04-09 Antivirals Inc. Morpholino-subunit combinatorial library and method
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5010175A (en) 1988-05-02 1991-04-23 The Regents Of The University Of California General method for producing and selecting peptides with specific properties
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
IE66205B1 (en) 1990-06-14 1995-12-13 Paul A Bartlett Polypeptide analogs
US5650489A (en) 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
AU2515992A (en) 1991-08-20 1993-03-16 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
EP0641781B1 (en) 1991-09-06 2000-07-26 Yoshitomi Pharmaceutical Industries, Ltd. 4-amino(alkyl)cyclohexane-1-carboxamide compound and use thereof
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
US5702932A (en) 1992-07-20 1997-12-30 University Of Florida Microinjection methods to transform arthropods with exogenous DNA
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
JPH09500534A (en) 1993-07-22 1997-01-21 メルク エンド カンパニー インコーポレーテッド Expression of human interleukin-1β in transgenic animals
US5519134A (en) 1994-01-11 1996-05-21 Isis Pharmaceuticals, Inc. Pyrrolidine-containing monomers and oligomers
US5593853A (en) 1994-02-09 1997-01-14 Martek Corporation Generation and screening of synthetic drug libraries
US5539083A (en) 1994-02-23 1996-07-23 Isis Pharmaceuticals, Inc. Peptide nucleic acid combinatorial libraries and improved methods of synthesis
US5656610A (en) 1994-06-21 1997-08-12 University Of Southern California Producing a protein in a mammal by injection of a DNA-sequence into the tongue
US5525735A (en) 1994-06-22 1996-06-11 Affymax Technologies Nv Methods for synthesizing diverse collections of pyrrolidine compounds
US5549974A (en) 1994-06-23 1996-08-27 Affymax Technologies Nv Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof
US5736524A (en) 1994-11-14 1998-04-07 Merck & Co.,. Inc. Polynucleotide tuberculosis vaccine
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5977322A (en) 1995-06-14 1999-11-02 The Regents Of The University Of California High affinity human antibodies to tumor antigens
US5569588A (en) 1995-08-09 1996-10-29 The Regents Of The University Of California Methods for drug screening
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
US5780448A (en) 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
GB9807935D0 (en) 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
US5945100A (en) 1996-07-31 1999-08-31 Fbp Corporation Tumor delivery vehicles
SI0956865T2 (en) 1996-08-12 2011-04-29 Mitsubishi Pharma Corp MEDICINES COMPRISING Rho KINASE INHIBITOR
US5981274A (en) 1996-09-18 1999-11-09 Tyrrell; D. Lorne J. Recombinant hepatitis virus vectors
JP4327260B2 (en) 1997-05-07 2009-09-09 フアルマ・マル・エセ・ア Apridin as an L-type calcium channel enhancer
DE69839444D1 (en) 1997-06-13 2008-06-19 Ludwig Inst Cancer Res SMAD 6 AND ITS APPLICATIONS
JP2002511092A (en) 1997-07-01 2002-04-09 ワーナー−ランバート・コンパニー 4-bromo or 4-iodophenylaminobenzhydroxamic acid derivatives and their use as MEK inhibitors
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US20060263382A1 (en) 1998-06-20 2006-11-23 Richard Hotchkiss Membrane-permeant peptide complexes for treatment of sepsis
CA2336220A1 (en) 1998-07-24 2000-02-03 The Carnegie Institution Of Washington Method for maintenance and propagation of germline stem cells using members of the tgf-.beta. family of growth factors
GB9819912D0 (en) 1998-09-11 1998-11-04 Univ Edinburgh Propagation and/or derivation of embryonic stem cells
DE69924641D1 (en) 1999-01-07 2005-05-12 Warner Lambert Company Llc Mor TREATMENT OF ASTHMA ON THE BASIS OF MEK INHIBITORS
US6703420B1 (en) 1999-03-19 2004-03-09 Bristol-Myers Squibb Pharma Company Amino-thio-acrylonitriles as MEK inhibitors
AU5250400A (en) 1999-06-18 2001-01-09 Mitsubishi Pharma Corporation Osteogenesis promoters
US6730293B1 (en) 1999-08-24 2004-05-04 Cellgate, Inc. Compositions and methods for treating inflammatory diseases of the skin
WO2001017562A1 (en) 1999-09-02 2001-03-15 Yamanouchi Pharmaceutical Co., Ltd. Osteogenesis promoting agents
US20020142457A1 (en) 1999-12-28 2002-10-03 Akihiro Umezawa Cell having the potentiality of differentiation into cardiomyocytes
US6692736B2 (en) 2000-03-24 2004-02-17 Cell Genesys, Inc. Cell-specific adenovirus vectors comprising an internal ribosome entry site
US6960614B2 (en) 2000-07-19 2005-11-01 Warner-Lambert Company Oxygenated esters of 4-lodo phenylamino benzhydroxamic acids
EP1370553B1 (en) 2001-03-23 2006-05-10 Bayer Corporation Rho-kinase inhibitors
MXPA03008659A (en) 2001-03-23 2005-04-08 Bayer Ag Rho-kinase inhibitors.
CA2446110C (en) 2001-05-01 2013-06-25 National Research Council Of Canada A system for inducible expression in eukaryotic cells
US20050130144A1 (en) 2001-09-21 2005-06-16 Norio Nakatsuji Method of screening reprogramming factor, reprogramming factor screened by the method, method of using the reprogramming factor, method of differentiating undifferentiated fused cells and method of constructing cell, tissues and organs
ATE554169T1 (en) 2001-11-02 2012-05-15 Giuliani Int Ltd SMAD7 INHIBITORS FOR THE TREATMENT OF CNS DISEASES
WO2003059913A1 (en) 2002-01-10 2003-07-24 Bayer Healthcare Ag Roh-kinase inhibitors
EP1470121B1 (en) 2002-01-23 2012-07-11 Bayer HealthCare LLC Pyrimidine derivatives as rho-kinase inhibitors
ATE381557T1 (en) 2002-01-23 2008-01-15 Bayer Pharmaceuticals Corp RHO KINASE INHIBITORS
WO2003073843A2 (en) 2002-03-05 2003-09-12 Artemis Pharmaceuticals Gmbh Inbred embryonic stem-cell derived mice
MXPA04008893A (en) 2002-03-13 2005-06-20 Array Biopharma Inc N3 alkylated benzimidazole derivatives as mek inhibitors.
JPWO2003082808A1 (en) 2002-04-03 2005-08-04 住友製薬株式会社 Benzamide derivatives
GB0210539D0 (en) 2002-05-08 2002-06-19 Univ Edinburgh Control of es cell self renewal and lineage specification, and medium therefor
WO2004039796A1 (en) 2002-10-28 2004-05-13 Bayer Healthcare Ag Heteroaryloxy-substituted phenylaminopyrimidines as rho-kinase inhibitors
EP1562601A1 (en) 2002-11-15 2005-08-17 Warner-Lambert Company LLC Combination chemotherapy comprising a mek inhibitor and capecitabine for treating cancer
JP2006517234A (en) 2003-02-10 2006-07-20 アムジエン・インコーポレーテツド Vanilloid receptor ligands and their use in therapy
AU2003901099A0 (en) 2003-03-11 2003-03-27 Es Cell International Pte Ltd. Methods of inducing differentiation of stem cells
US20050075276A1 (en) 2003-03-14 2005-04-07 Christopher Rudd Use of inhibitors of glycogen synthase-3 to augment CD28 dependent -T-cell responses
US20070010008A1 (en) 2005-06-29 2007-01-11 Tissuetech, Inc. Ex vivo expansion of primary animal cells
US20060222657A1 (en) 2003-06-20 2006-10-05 Dowdy Steven F Polypeptide transduction and fusogenic peptides
JP4684108B2 (en) 2003-10-03 2011-05-18 恵一 福田 Method for inducing differentiation of cardiomyocytes from stem cells
EP2468729B1 (en) 2003-10-15 2013-12-25 Ube Industries, Ltd. Novel indazole derivative
US20070178439A1 (en) 2003-10-16 2007-08-02 Smith Austin G Control of es cell self renewal and lineage specification, and medium therefor
BRPI0416692A (en) 2003-11-19 2007-01-30 Array Biopharma Inc heterocyclic mek inhibitors and methods of using these
US20070141703A1 (en) 2003-11-19 2007-06-21 Stanley Edouard G Methods for producing blood products from pluripotent cells in cell culture
US20070269412A1 (en) 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
EP1713775A4 (en) 2004-01-30 2009-08-12 Smithkline Beecham Corp Chemical compounds
DK1740601T3 (en) 2004-04-21 2013-03-11 Univ Chicago Myosin light chain kinase inhibitors and their use
WO2006020919A2 (en) 2004-08-13 2006-02-23 University Of Georgia Research Foundation, Inc. Compositions and methods for self-renewal and differentiation in human embryonic stem cells
WO2006026473A2 (en) 2004-08-25 2006-03-09 University Of Georgia Research Foundation, Inc. METHODS AND COMPOSITIONS UTILIZING MYC AND GSK3ß TO MANIPULATE THE PLURIPOTENCY OF EMBRYONIC STEM CELLS
US20060182724A1 (en) 2005-02-15 2006-08-17 Riordan Neil H Method for expansion of stem cells
NZ563707A (en) 2005-05-18 2011-01-28 Array Biopharma Inc Heterocyclic inhibitors of MEK and methods of use thereof
JP4395193B2 (en) 2005-08-01 2010-01-06 ニューポテンシャル，インコーポレイテッド Production of reprogrammed cells with ability to recover
US20080242594A1 (en) 2005-09-08 2008-10-02 Mckay Ronald D G Methods for Promoting Stem Cell Proliferation and Survival
US8217042B2 (en) 2005-11-11 2012-07-10 Zentaris Gmbh Pyridopyrazines and their use as modulators of kinases
ZA200804673B (en) 2005-12-13 2009-11-25 Univ Kyoto Nuclear reprogramming factor
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
GB0601538D0 (en) 2006-01-26 2006-03-08 Univ Birmingham Epigenetic analysis
WO2007088651A1 (en) 2006-02-01 2007-08-09 The University Of Tokyo JOINT USE OF TGF-β SIGNAL INHIBITOR AND ANTITUMOR AGENT
US7541186B2 (en) 2006-02-22 2009-06-02 University Of Washington Method of generating human retinal progenitors from embryonic stem cells
GB0615327D0 (en) 2006-03-30 2006-09-13 Univ Edinburgh Culture medium containing kinase inhibitors and uses thereof
WO2007113505A2 (en) 2006-03-30 2007-10-11 The University Court Of The University Of Edinburgh Culture medium containing kinase inhibitors. and uses thereof
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
CA2650561C (en) 2006-05-02 2014-02-25 Wisconsin Alumni Research Foundation Method of differentiating stem cells into cells of the endoderm and pancreatic lineage
BRPI0713119A2 (en) 2006-06-30 2012-04-17 Schering Corp Substituted piperidines that increase the activity of p53 and the uses of these
ES2393932T3 (en) 2006-07-14 2013-01-02 Novartis Ag Pyrimidine derivatives as ALK-5 inhibitors
US20080020014A1 (en) 2006-07-19 2008-01-24 Paul Consigny Implantable devices containing nuclear receptor ligands for the treatment of vascular and related disorders
ZA200901914B (en) 2006-09-22 2010-06-30 Riken Stem cell culture medium and method
JP2008099662A (en) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research Stem cell culture method
GB0622395D0 (en) 2006-11-09 2006-12-20 Univ Cambridge Tech Methods relating to pluripotent cells
JP5419279B2 (en) 2007-01-17 2014-02-19 ウィスコンシン アラムニ リサーチ ファンデーション Improved stem cell culture
US20100172883A1 (en) 2007-01-19 2010-07-08 Bruneau Benoit Gaetan Methods of generating cardiomyocytes
EP2126045A4 (en) 2007-01-30 2010-05-26 Univ Georgia Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (mmc)
KR20090122946A (en) 2007-02-27 2009-12-01 주식회사 프로셀제약 Combined use of cell permeable NANO and OCT4 to promote stem cell self-renewal and inhibit differentiation
EP3449923A1 (en) 2007-03-07 2019-03-06 MEI Pharma, Inc. Combination of benzimidazole anti-cancer agent and a second anti-cancer agent
WO2008126932A2 (en) 2007-04-09 2008-10-23 Riken Epigenetical regulation of brain plasticity
CN101688178B (en) 2007-04-18 2013-12-04 哈达锡特医学研究服务及发展有限公司 Stem cell-derived retinal pigment epithelial cells
US7878210B2 (en) 2007-06-04 2011-02-01 Philip Morris Usa Inc. Cellulose acetate fiber modification
US8648069B2 (en) 2007-06-08 2014-02-11 Abbvie Inc. 5-substituted indazoles as kinase inhibitors
JP2008307007A (en) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
CA2691793A1 (en) 2007-06-29 2009-01-08 Cellular Dynamics International, Inc. Automated method and apparatus for embryonic stem cell culture
US20120282229A1 (en) 2007-08-01 2012-11-08 Christian Kannemeier Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
MX2010002242A (en) 2007-08-31 2010-06-01 Whitehead Biomedical Inst Wnt pathway stimulation in reprogramming somatic cells.
CA2660123C (en) 2007-10-31 2017-05-09 Kyoto University Nuclear reprogramming method
WO2009061442A1 (en) 2007-11-06 2009-05-14 Children's Medical Center Corporation Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells
JP4604076B2 (en) 2007-11-07 2010-12-22 国立大学法人静岡大学 Electrolyte membrane for fuel cell
WO2009073523A2 (en) 2007-11-29 2009-06-11 Children's Hospital Of Orange County De-differentiation of human cells
WO2009067756A1 (en) 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving sox2 protein
WO2009067757A1 (en) 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving oct4 protein
JP2011516042A (en) 2008-03-17 2011-05-26 へルムホルツ・ツェントルム・ミュンヘン−ドイチェス・フォルシュングスツェントルム・ヒューア・ゲズントハイト・ウント・ウムヴェルト（ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング） Vector and method for producing vector-free induced pluripotent stem (iPS) cells using site-specific recombination
SG188904A1 (en) 2008-03-17 2013-04-30 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
CN101550406B (en) 2008-04-03 2016-02-10 北京大学 Prepare the method for pluripotent stem cell, test kit and purposes
CN101848994A (en) 2008-07-30 2010-09-29 国立大学法人京都大学 Method for efficiently establishing induced pluripotent stem cells
US8298825B1 (en) 2008-08-25 2012-10-30 The General Hospital Corporation TGF-beta receptor inhibitors to enhance direct reprogramming
ES2743204T3 (en) 2008-11-04 2020-02-18 Viacyte Inc Suspension compositions of stem cell aggregates and methods for differentiation
ES2712074T3 (en) 2008-12-03 2019-05-09 Scripps Research Inst Stem cell cultures
EP3312269A1 (en) 2008-12-17 2018-04-25 The Scripps Research Institute Generation and maintenance of stem cells
US20100150889A1 (en) * 2008-12-17 2010-06-17 The Uab Research Foundation Polycistronic Vector For Human Induced Pluripotent Stem Cell Production
WO2010102267A2 (en) 2009-03-06 2010-09-10 Ipierian, Inc. Tgf-beta pathway inhibitors for enhancement of cellular reprogramming of human cells
WO2010115052A2 (en) * 2009-04-03 2010-10-07 The Mclean Hospital Corporation Induced pluripotent stem cells
WO2010120785A2 (en) 2009-04-13 2010-10-21 The Regents Of The University Of California Methods and compositions for stem cell cultures
EP2435558A4 (en) 2009-05-27 2013-05-22 Salk Inst For Biological Studi PRODUCTION OF HEAVY INDUCED PLURIPOTENT STEM CELLS GENERALLY CORRECTED
JP5777113B2 (en) 2009-05-29 2015-09-09 学校法人慶應義塾 Method for selecting induced pluripotent stem cell clones
US8524498B2 (en) 2009-05-29 2013-09-03 The General Hospital Corporation Methods and compositions for homologous recombination in human cells
KR101813464B1 (en) 2009-06-05 2018-01-30 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 Reprogramming T cells and hematopoietic cells
AU2010279913B2 (en) 2009-08-07 2016-04-28 Kyoto University Method of efficiently establishing induced pluripotent stem cells
CN105861446B (en) 2009-10-16 2021-10-01 斯克里普斯研究所 Induction of pluripotent cells
MX390753B (en) 2009-10-31 2025-03-21 Genesis Tech Limited METHODS FOR REPROGRAMMING CELLS AND THEIR USES.
WO2011056971A2 (en) 2009-11-04 2011-05-12 Cellular Dynamics International, Inc. Episomal reprogramming with chemicals
ES2779048T3 (en) 2009-11-12 2020-08-13 Technion Res & Dev Foundation Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
WO2011090221A1 (en) 2010-01-22 2011-07-28 Kyoto University Method for improving induced pluripotent stem cell generation efficiency
CA2789774C (en) 2010-02-17 2024-01-23 Biotime Inc. Methods for telomere length and genomic dna quality control analysis in pluripotent stem cells
ES2533581T3 (en) 2010-03-05 2015-04-13 Texas Heart Institute ETS2 and Mesp1 generators of cardiac progenitors from fibroblasts
EP3199623B1 (en) 2010-03-31 2021-07-28 The Scripps Research Institute Reprogramming cells
CA2802249A1 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
US20110306516A1 (en) 2010-06-15 2011-12-15 The New York Stem Cell Foundation Methods for producing induced pluripotent stem cells
WO2011158852A1 (en) 2010-06-15 2011-12-22 国立大学法人東京大学 Process for production of induced pluripotent stem cell
EP2601289B1 (en) 2010-08-04 2017-07-12 Cellular Dynamics International, Inc. Reprogramming immortalized b cells
KR20200113286A (en) 2010-12-22 2020-10-06 페이트 세러퓨틱스, 인코포레이티드 Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
WO2013070852A2 (en) * 2011-11-08 2013-05-16 Emory University Compounds and compositions used to epigenetically transform cells and methods related thereto
HK1203081A1 (en) 2011-12-08 2015-10-16 Fred Hutchinson Cancer Research Center Compositions and methods for enhanced generation of hematopoietic stem/progenitor cells
WO2013159103A1 (en) * 2012-04-20 2013-10-24 Whitehead Institute For Biomedical Research Programming and reprogramming of cells
WO2013176197A1 (en) 2012-05-22 2013-11-28 国立大学法人 東京大学 Method for producing antigen-specific t cells
US9166832B1 (en) 2013-10-04 2015-10-20 Altera Corporation Methods and apparatus for decision feedback equalization adaptation
KR102460549B1 (en) 2014-03-04 2022-10-28 페이트 세러퓨틱스, 인코포레이티드 Improved reprogramming methods and cell culture platforms
KR20250007036A (en) 2015-10-16 2025-01-13 페이트 세러퓨틱스, 인코포레이티드 Platform for the induction and maintenance of ground state pluripotency
CN115806940A (en) 2015-11-04 2023-03-17 菲特治疗公司 Genome engineering of pluripotent cells

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