DK4077713T3 - Fremgangsmåde og kit til amplifikation af hele genomet og analyse af målmolekyler i en biologisk prøve - Google Patents
Fremgangsmåde og kit til amplifikation af hele genomet og analyse af målmolekyler i en biologisk prøve Download PDFInfo
- Publication number
- DK4077713T3 DK4077713T3 DK20829359.7T DK20829359T DK4077713T3 DK 4077713 T3 DK4077713 T3 DK 4077713T3 DK 20829359 T DK20829359 T DK 20829359T DK 4077713 T3 DK4077713 T3 DK 4077713T3
- Authority
- DK
- Denmark
- Prior art keywords
- kit
- analysis
- biological sample
- target molecules
- whole genome
- Prior art date
Links
- 238000004458 analytical method Methods 0.000 title 1
- 239000012472 biological sample Substances 0.000 title 1
- 238000013412 genome amplification Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT102019000024159A IT201900024159A1 (it) | 2019-12-16 | 2019-12-16 | Metodo e kit per amplificazione totale del genoma e analisi di molecole bersaglio in un campione biologico |
| PCT/IB2020/062053 WO2021124166A1 (en) | 2019-12-16 | 2020-12-16 | Method and kit for whole genome amplification and analysis of target molecules in a biological sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK4077713T3 true DK4077713T3 (da) | 2023-08-28 |
| DK4077713T5 DK4077713T5 (da) | 2024-07-22 |
Family
ID=69904109
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK20829359.7T DK4077713T5 (da) | 2019-12-16 | 2020-12-16 | Fremgangsmåde og kit til amplifikation af hele genomet og analyse af målmolekyler i en biologisk prøve |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US20230053146A1 (da) |
| EP (1) | EP4077713B1 (da) |
| JP (1) | JP2023511492A (da) |
| KR (1) | KR20220139859A (da) |
| CN (1) | CN114867867A (da) |
| AU (1) | AU2020408301A1 (da) |
| CA (1) | CA3161615A1 (da) |
| DK (1) | DK4077713T5 (da) |
| ES (1) | ES2959838T3 (da) |
| FI (1) | FI4077713T3 (da) |
| IL (1) | IL293720B2 (da) |
| IT (1) | IT201900024159A1 (da) |
| PL (1) | PL4077713T3 (da) |
| PT (1) | PT4077713T (da) |
| WO (1) | WO2021124166A1 (da) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2024509984A (ja) * | 2021-03-19 | 2024-03-05 | パリ シアンス エ レットル | 細胞又は細胞小器官の選択的分析のための方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6673541B1 (en) | 1998-09-18 | 2004-01-06 | Micromet Ag | DNA amplification of a single cell |
| JP5954876B2 (ja) | 2009-10-13 | 2016-07-20 | ナノストリング テクノロジーズ, インコーポレイテッド | ナノレポーターによるタンパク質の検出 |
| CN104471077B (zh) * | 2012-05-21 | 2017-05-24 | 富鲁达公司 | 颗粒群的单颗粒分析 |
| ITTO20120962A1 (it) * | 2012-10-31 | 2014-05-01 | Silicon Biosystems Spa | Metodo e kit per rivelare una sequenza di dna bersaglio wild-type e/o mutata |
| EP2746405B1 (en) * | 2012-12-23 | 2015-11-04 | HS Diagnomics GmbH | Methods and primer sets for high throughput PCR sequencing |
| PT2881739T (pt) * | 2013-12-04 | 2017-08-11 | Fraunhofer Ges Forschung | Método e estojo para determinação da integridade do genoma e/ou da qualidade de uma biblioteca de sequências de adn obtida através de amplificação determinística do genoma inteiro por locais de restrição |
| US9745611B2 (en) * | 2014-05-06 | 2017-08-29 | Genewiz Inc. | Methods and kits for identifying microorganisms in a sample |
| ITUA20162640A1 (it) | 2016-04-15 | 2017-10-15 | Menarini Silicon Biosystems Spa | Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo |
| US10011872B1 (en) * | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20180251825A1 (en) | 2017-02-02 | 2018-09-06 | New York Genome Center Inc. | Methods and compositions for identifying or quantifying targets in a biological sample |
| EP3431611A1 (en) | 2017-07-21 | 2019-01-23 | Menarini Silicon Biosystems S.p.A. | Improved method and kit for the generation of dna libraries for massively parallel sequencing |
| IL271454B2 (en) * | 2018-05-17 | 2025-04-01 | Illumina Inc | Rapid single-cell genetic sequencing with low Aggregation bias |
| US11414701B2 (en) * | 2018-05-24 | 2022-08-16 | The Broad Institute, Inc. | Multimodal readouts for quantifying and sequencing nucleic acids in single cells |
-
2019
- 2019-12-16 IT IT102019000024159A patent/IT201900024159A1/it unknown
-
2020
- 2020-12-16 DK DK20829359.7T patent/DK4077713T5/da active
- 2020-12-16 AU AU2020408301A patent/AU2020408301A1/en active Pending
- 2020-12-16 EP EP20829359.7A patent/EP4077713B1/en active Active
- 2020-12-16 KR KR1020227023430A patent/KR20220139859A/ko active Pending
- 2020-12-16 FI FIEP20829359.7T patent/FI4077713T3/fi active
- 2020-12-16 IL IL293720A patent/IL293720B2/en unknown
- 2020-12-16 JP JP2022536964A patent/JP2023511492A/ja active Pending
- 2020-12-16 PL PL20829359.7T patent/PL4077713T3/pl unknown
- 2020-12-16 CA CA3161615A patent/CA3161615A1/en active Pending
- 2020-12-16 US US17/786,464 patent/US20230053146A1/en active Pending
- 2020-12-16 WO PCT/IB2020/062053 patent/WO2021124166A1/en not_active Ceased
- 2020-12-16 ES ES20829359T patent/ES2959838T3/es active Active
- 2020-12-16 CN CN202080087910.1A patent/CN114867867A/zh active Pending
- 2020-12-16 PT PT208293597T patent/PT4077713T/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2020408301A1 (en) | 2022-06-30 |
| IL293720B1 (en) | 2023-10-01 |
| FI4077713T3 (fi) | 2023-09-27 |
| KR20220139859A (ko) | 2022-10-17 |
| EP4077713B1 (en) | 2023-07-26 |
| PL4077713T3 (pl) | 2024-01-03 |
| CA3161615A1 (en) | 2021-06-24 |
| EP4077713A1 (en) | 2022-10-26 |
| ES2959838T3 (es) | 2024-02-28 |
| IT201900024159A1 (it) | 2021-06-16 |
| JP2023511492A (ja) | 2023-03-20 |
| IL293720A (en) | 2022-08-01 |
| CN114867867A (zh) | 2022-08-05 |
| US20230053146A1 (en) | 2023-02-16 |
| DK4077713T5 (da) | 2024-07-22 |
| PT4077713T (pt) | 2023-08-18 |
| IL293720B2 (en) | 2024-02-01 |
| WO2021124166A1 (en) | 2021-06-24 |
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