DK3957744T3 - Reagenser og fremgangsmåder til molekylær stregkodning af enkeltcellenukleinsyrer - Google Patents
Reagenser og fremgangsmåder til molekylær stregkodning af enkeltcellenukleinsyrer Download PDFInfo
- Publication number
- DK3957744T3 DK3957744T3 DK21168174.7T DK21168174T DK3957744T3 DK 3957744 T3 DK3957744 T3 DK 3957744T3 DK 21168174 T DK21168174 T DK 21168174T DK 3957744 T3 DK3957744 T3 DK 3957744T3
- Authority
- DK
- Denmark
- Prior art keywords
- reagents
- methods
- nucleic acids
- single cell
- cell nucleic
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1622222.6A GB201622222D0 (en) | 2016-12-23 | 2016-12-23 | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| EP17817859.6A EP3559267B1 (en) | 2016-12-23 | 2017-12-19 | Method for molecular barcoding of nucleic acids of single cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK3957744T3 true DK3957744T3 (da) | 2023-09-18 |
| DK3957744T5 DK3957744T5 (da) | 2024-07-15 |
Family
ID=58360450
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK21168174.7T DK3957744T5 (da) | 2016-12-23 | 2017-12-19 | Reagenser og fremgangsmåder til molekylær stregkodning af enkeltcellenukleinsyrer |
| DK17817859.6T DK3559267T3 (da) | 2016-12-23 | 2017-12-19 | Fremgangsmåde til molekylær stregkodning af nukleinsyrer i enkeltceller |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17817859.6T DK3559267T3 (da) | 2016-12-23 | 2017-12-19 | Fremgangsmåde til molekylær stregkodning af nukleinsyrer i enkeltceller |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US12084714B2 (da) |
| EP (3) | EP3559267B1 (da) |
| DK (2) | DK3957744T5 (da) |
| ES (2) | ES2955997T3 (da) |
| GB (1) | GB201622222D0 (da) |
| HR (1) | HRP20211078T1 (da) |
| SI (1) | SI3559267T1 (da) |
| WO (1) | WO2018115852A1 (da) |
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| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9719068B2 (en) | 2010-05-06 | 2017-08-01 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
| AU2013226081B2 (en) | 2012-02-27 | 2018-06-14 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
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| EP3149156B1 (en) | 2014-05-28 | 2021-02-17 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into gastric tissues through directed differentiation |
| EP3207123A1 (en) | 2014-10-17 | 2017-08-23 | Children's Hospital Center D/b/a Cincinnati Children's Hospital Medical Center | In vivo model of human small intestine using pluripotent stem cells and methods of making and using same |
| EP3262192B1 (en) | 2015-02-27 | 2020-09-16 | Becton, Dickinson and Company | Spatially addressable molecular barcoding |
| EP4180535A1 (en) | 2015-03-30 | 2023-05-17 | Becton, Dickinson and Company | Methods and compositions for combinatorial barcoding |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
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| US11066650B2 (en) | 2016-05-05 | 2021-07-20 | Children's Hospital Medical Center | Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same |
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| CA3034924A1 (en) | 2016-09-26 | 2018-03-29 | Cellular Research, Inc. | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| EP4553082A3 (en) | 2016-11-04 | 2025-08-20 | Children's Hospital Medical Center | Liver organoid compositions and methods of making and using same |
| KR102807995B1 (ko) | 2016-12-05 | 2025-05-16 | 칠드런즈 호스피탈 메디칼 센터 | 결장 유사장기 및 이를 제조 및 사용하는 방법 |
| GB201622222D0 (en) | 2016-12-23 | 2017-02-08 | Cs Genetics Ltd | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| US11319583B2 (en) | 2017-02-01 | 2022-05-03 | Becton, Dickinson And Company | Selective amplification using blocking oligonucleotides |
| WO2018191673A1 (en) | 2017-04-14 | 2018-10-18 | Children's Hospital Medical Center | Multi donor stem cell compositions and methods of making same |
| KR102790050B1 (ko) | 2017-06-05 | 2025-04-04 | 백톤 디킨슨 앤드 컴퍼니 | 단일 세포를 위한 샘플 인덱싱 |
| CN111565798B (zh) | 2017-10-10 | 2025-06-24 | 儿童医院医学中心 | 食道组织和/或类器官组合物及其制备方法 |
| US12379372B2 (en) | 2017-12-21 | 2025-08-05 | Children's Hospital Medical Center | Digitalized human organoids and methods of using same |
| ES3014208T3 (en) | 2018-05-03 | 2025-04-21 | Becton Dickinson Co | Molecular barcoding on opposite transcript ends |
| EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
| US12421500B2 (en) | 2018-07-26 | 2025-09-23 | Children's Hospital Medical Center | Hepato-biliary-pancreatic tissues and methods of making same |
| KR20210057781A (ko) | 2018-09-12 | 2021-05-21 | 칠드런즈 호스피탈 메디칼 센터 | 조혈 줄기세포 및 이의 파생체의 생산을 위한 오르가노이드 조성물 |
| CN118853827A (zh) | 2018-10-01 | 2024-10-29 | 贝克顿迪金森公司 | 确定5’转录物序列 |
| JP7618548B2 (ja) | 2018-11-08 | 2025-01-21 | ベクトン・ディキンソン・アンド・カンパニー | ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析 |
| WO2020123384A1 (en) | 2018-12-13 | 2020-06-18 | Cellular Research, Inc. | Selective extension in single cell whole transcriptome analysis |
| EP4242322B1 (en) | 2019-01-23 | 2024-08-21 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
| EP3924506A1 (en) | 2019-02-14 | 2021-12-22 | Becton Dickinson and Company | Hybrid targeted and whole transcriptome amplification |
| MX2021003746A (es) * | 2019-03-01 | 2021-06-23 | Illumina Inc | Genotecas de núcleos únicos y células únicas de alto rendimiento y métodos para elaborarlas y usarlas. |
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| CN114051534B (zh) | 2019-07-22 | 2025-02-21 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| CN114729350A (zh) | 2019-11-08 | 2022-07-08 | 贝克顿迪金森公司 | 使用随机引发获得用于免疫组库测序的全长v(d)j信息 |
| EP4090763B1 (en) | 2020-01-13 | 2024-12-04 | Becton Dickinson and Company | Methods and compositions for quantitation of proteins and rna |
| EP4471155A3 (en) | 2020-01-29 | 2024-12-18 | Becton, Dickinson and Company | Barcoded wells for spatial mapping of single cells through sequencing |
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| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
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| GB201622222D0 (en) | 2016-12-23 | 2017-02-08 | Cs Genetics Ltd | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| EP4345159A3 (en) | 2017-05-05 | 2024-06-05 | Scipio Bioscience | Methods for trapping and barcoding discrete biological units in hydrogel |
-
2016
- 2016-12-23 GB GBGB1622222.6A patent/GB201622222D0/en not_active Ceased
-
2017
- 2017-12-19 DK DK21168174.7T patent/DK3957744T5/da active
- 2017-12-19 ES ES21168174T patent/ES2955997T3/es active Active
- 2017-12-19 US US16/471,941 patent/US12084714B2/en active Active
- 2017-12-19 ES ES17817859T patent/ES2880394T3/es active Active
- 2017-12-19 DK DK17817859.6T patent/DK3559267T3/da active
- 2017-12-19 EP EP17817859.6A patent/EP3559267B1/en active Active
- 2017-12-19 SI SI201730847T patent/SI3559267T1/sl unknown
- 2017-12-19 WO PCT/GB2017/053816 patent/WO2018115852A1/en not_active Ceased
- 2017-12-19 HR HRP20211078TT patent/HRP20211078T1/hr unknown
- 2017-12-19 EP EP21168174.7A patent/EP3957744B1/en active Active
- 2017-12-19 EP EP23181491.4A patent/EP4265723B1/en active Active
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2023
- 2023-03-17 US US18/185,904 patent/US11845924B1/en active Active
- 2023-11-06 US US18/502,977 patent/US20240327896A1/en active Pending
-
2024
- 2024-08-02 US US18/793,425 patent/US20250207176A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| ES2955997T3 (es) | 2023-12-11 |
| EP4265723A2 (en) | 2023-10-25 |
| US20250207176A1 (en) | 2025-06-26 |
| DK3957744T5 (da) | 2024-07-15 |
| GB201622222D0 (en) | 2017-02-08 |
| US12084714B2 (en) | 2024-09-10 |
| EP3957744A1 (en) | 2022-02-23 |
| EP3559267A1 (en) | 2019-10-30 |
| EP4265723B1 (en) | 2025-10-08 |
| EP3559267B1 (en) | 2021-04-14 |
| EP3957744B1 (en) | 2023-06-28 |
| US20240327896A1 (en) | 2024-10-03 |
| HRP20211078T1 (hr) | 2021-10-01 |
| US11845924B1 (en) | 2023-12-19 |
| ES2880394T3 (es) | 2021-11-24 |
| DK3559267T3 (da) | 2021-07-12 |
| US20190316182A1 (en) | 2019-10-17 |
| EP4265723A3 (en) | 2024-01-10 |
| SI3559267T1 (sl) | 2021-08-31 |
| WO2018115852A1 (en) | 2018-06-28 |
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