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DK3874047T3 - Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle - Google Patents

Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle Download PDF

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Publication number
DK3874047T3
DK3874047T3 DK20700131.4T DK20700131T DK3874047T3 DK 3874047 T3 DK3874047 T3 DK 3874047T3 DK 20700131 T DK20700131 T DK 20700131T DK 3874047 T3 DK3874047 T3 DK 3874047T3
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DK
Denmark
Prior art keywords
host cell
carbon source
recombinant host
protein production
regulated protein
Prior art date
Application number
DK20700131.4T
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English (en)
Other versions
DK3874047T4 (da
Inventor
Brigitte Gasser
Corinna Rebnegger
Villegas Mirelle Citlali Flores
Diethard Mattanovich
Original Assignee
Lonza Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=65268724&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK3874047(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lonza Ag filed Critical Lonza Ag
Publication of DK3874047T3 publication Critical patent/DK3874047T3/da
Application granted granted Critical
Publication of DK3874047T4 publication Critical patent/DK3874047T4/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK20700131.4T 2019-01-11 2020-01-10 Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle DK3874047T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19151376 2019-01-11
PCT/EP2020/050517 WO2020144313A1 (en) 2019-01-11 2020-01-10 Carbon-source regulated protein production in a recombinant host cell

Publications (2)

Publication Number Publication Date
DK3874047T3 true DK3874047T3 (da) 2022-06-20
DK3874047T4 DK3874047T4 (da) 2025-02-03

Family

ID=65268724

Family Applications (1)

Application Number Title Priority Date Filing Date
DK20700131.4T DK3874047T4 (da) 2019-01-11 2020-01-10 Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle

Country Status (11)

Country Link
US (2) US11773424B2 (da)
EP (1) EP3874047B2 (da)
JP (1) JP7061234B2 (da)
KR (2) KR102699074B1 (da)
CN (2) CN116904331A (da)
CA (1) CA3125217C (da)
DK (1) DK3874047T4 (da)
ES (1) ES2921137T5 (da)
SG (1) SG11202105667RA (da)
TW (1) TWI853878B (da)
WO (1) WO2020144313A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4222266A1 (en) 2020-09-30 2023-08-09 Lonza Ltd. Host cells overexpressing translational factors
CN113764034B (zh) * 2021-08-03 2023-09-22 腾讯科技（深圳）有限公司 基因组序列中潜在bgc的预测方法、装置、设备及介质
CN114657190B (zh) * 2022-04-06 2023-08-29 暨南大学 Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用
CN115386009B (zh) * 2022-04-26 2023-12-01 江苏靶标生物医药研究所有限公司 一种膜联蛋白v与血管生成抑制剂融合蛋白的构建方法和应用
CN115716868B (zh) * 2022-08-01 2024-01-26 华中农业大学 转录因子MrPigB突变体及其应用
CN116041426B (zh) * 2022-08-18 2023-11-03 齐齐哈尔大学 玉米蛋白源抗粘附肽及其制备方法和应用
CN116134050B (zh) * 2022-09-02 2025-03-28 通化安睿特生物制药股份有限公司 一种提高重组人白蛋白表达量的方法和细胞和蛋白
WO2024133538A1 (en) 2022-12-20 2024-06-27 Lonza Ltd Host cells with pat1 knockout for increased specific protein productivity

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2177528T3 (es) 1991-04-01 2002-12-16 Merck & Co Inc Genes que influyen en la actividad proteolitica de pichia y usoso de los mismos.
US6153424A (en) 1995-11-09 2000-11-28 Zymogenetics, Inc. Protease-deficient strains of Pichia methanolica
AU2008241061A1 (en) 2007-04-20 2008-10-30 Polymun Scientific Immunbiologische Forschung Gmbh Yeast expression systems
JP2012518999A (ja) 2009-02-26 2012-08-23 グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー 宿主細胞およびその使用方法
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
WO2012102171A1 (ja) 2011-01-27 2012-08-02 株式会社カネカ 形質転換用酵母およびタンパク質の製造方法
EP3590950A1 (en) 2011-05-09 2020-01-08 Ablynx NV Method for the production of immunoglobulin single varible domains
WO2013050551A1 (en) 2011-10-07 2013-04-11 Lonza Ltd Regulatable promoter
AU2013341049B2 (en) 2012-10-29 2017-07-20 Lonza Ltd Expression sequences
US10590427B2 (en) * 2014-04-17 2020-03-17 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell for expressing proteins of interest
SG11201608084TA (en) * 2014-04-17 2016-10-28 Boehringer Ingelheim Rcv Gmbh Recombinant host cell engineered to overexpress helper proteins
EP2952584A1 (en) 2014-06-04 2015-12-09 Boehringer Ingelheim RCV GmbH & Co KG Improved protein production
WO2017021541A1 (en) 2015-08-05 2017-02-09 Lonza Ltd Promoter variants

Also Published As

Publication number Publication date
JP7061234B2 (ja) 2022-04-27
EP3874047A1 (en) 2021-09-08
KR20230022260A (ko) 2023-02-14
CN116904331A (zh) 2023-10-20
US20220042064A1 (en) 2022-02-10
ES2921137T5 (en) 2025-05-06
WO2020144313A1 (en) 2020-07-16
US11773424B2 (en) 2023-10-03
EP3874047B1 (en) 2022-04-06
KR102699074B1 (ko) 2024-08-26
CN113423836B (zh) 2023-07-11
JP2022508470A (ja) 2022-01-19
SG11202105667RA (en) 2021-07-29
ES2921137T3 (es) 2022-08-18
DK3874047T4 (da) 2025-02-03
CA3125217A1 (en) 2020-07-16
CA3125217C (en) 2023-01-17
TW202031898A (zh) 2020-09-01
TWI853878B (zh) 2024-09-01
CN113423836A (zh) 2021-09-21
KR20210114401A (ko) 2021-09-23
KR102495283B1 (ko) 2023-02-06
EP3874047B2 (en) 2024-12-25
US20240018563A1 (en) 2024-01-18

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