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DK3527667T3 - Gærpromotorer til proteinekspression - Google Patents

Gærpromotorer til proteinekspression Download PDF

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Publication number
DK3527667T3
DK3527667T3 DK19150337.4T DK19150337T DK3527667T3 DK 3527667 T3 DK3527667 T3 DK 3527667T3 DK 19150337 T DK19150337 T DK 19150337T DK 3527667 T3 DK3527667 T3 DK 3527667T3
Authority
DK
Denmark
Prior art keywords
protein expression
yeast promoters
promoters
yeast
protein
Prior art date
Application number
DK19150337.4T
Other languages
English (en)
Inventor
Ilya I Tolstorukov
James M Cregg
Thomas G Chappell
Knut R Madden
Original Assignee
Biogrammatics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogrammatics Inc filed Critical Biogrammatics Inc
Application granted granted Critical
Publication of DK3527667T3 publication Critical patent/DK3527667T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/60Vectors containing traps for, e.g. exons, promoters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01022Alpha-galactosidase (3.2.1.22)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK19150337.4T 2013-03-08 2014-03-07 Gærpromotorer til proteinekspression DK3527667T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361775029P 2013-03-08 2013-03-08
EP14760869.9A EP2964775B1 (en) 2013-03-08 2014-03-07 Yeast promoters for protein expression

Publications (1)

Publication Number Publication Date
DK3527667T3 true DK3527667T3 (da) 2021-02-22

Family

ID=51492025

Family Applications (2)

Application Number Title Priority Date Filing Date
DK19150337.4T DK3527667T3 (da) 2013-03-08 2014-03-07 Gærpromotorer til proteinekspression
DK14760869.9T DK2964775T3 (da) 2013-03-08 2014-03-07 Gærpromotorer til proteinekspression

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK14760869.9T DK2964775T3 (da) 2013-03-08 2014-03-07 Gærpromotorer til proteinekspression

Country Status (10)

Country Link
US (3) US10676750B2 (da)
EP (3) EP3839055B1 (da)
CN (1) CN105358706A (da)
BR (1) BR112015021785A2 (da)
CA (1) CA2904333C (da)
DK (2) DK3527667T3 (da)
ES (3) ES2856265T3 (da)
HU (1) HUE042897T2 (da)
PT (1) PT2964775T (da)
WO (1) WO2014138679A1 (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3527667T3 (da) 2013-03-08 2021-02-22 Biogrammatics Inc Gærpromotorer til proteinekspression
DK2964765T3 (da) 2013-03-08 2019-07-29 Keck Graduate Inst Of Applied Life Sciences Gærpromotorer fra pichia pastoris
US11518797B2 (en) 2014-11-11 2022-12-06 Clara Foods Co. Methods and compositions for egg white protein production
WO2017058884A2 (en) 2015-09-28 2017-04-06 The Trustees Of Columbia University In The City Of New York Modified bacterial protein expression system
CN109536395A (zh) * 2018-02-07 2019-03-29 四川润格生物科技有限公司 一种高表达β-甘露聚糖酶的毕赤酵母工程菌株及应用
KR102131882B1 (ko) * 2018-02-14 2020-08-06 씨제이제일제당 주식회사 효모를 이용하여 이취가 개선된 발효 조성물의 제조방법
LT3997118T (lt) 2019-07-11 2025-05-26 Clara Foods Co. Gėrimo kompozicija, apimanti rekombinantinio ovomukoido baltymą
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
WO2022115636A1 (en) * 2020-11-25 2022-06-02 Esperovax Inc. Cell viability switch for genetically modified organisms
WO2023184539A1 (zh) * 2022-04-02 2023-10-05 武汉远大弘元股份有限公司 一种甲醇诱导启动子及其在制备丙谷二肽中的应用
CN119464353B (zh) * 2025-01-09 2025-08-19 上海昌进生物科技有限公司 生产α-葡萄糖苷酶的菌株及其构建方法和应用

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US14716A (en) * 1856-04-22 Machine for digging peat
US14715A (en) * 1856-04-22 Improvement in cultivators
US7078035B2 (en) 1997-08-13 2006-07-18 Diversa Corporation Phytases, nucleic acids encoding them and methods for making and using them
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
DK1165806T3 (da) 1999-03-31 2005-12-05 Cornell Res Foundation Inc Phosphataser med forbedret phytaseaktivitet
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
GB0126164D0 (en) 2001-10-31 2002-01-02 Danisco Sequences
PT2335501T (pt) 2001-10-31 2016-10-28 Huvepharma Ad Alimento animal contendo fitase e método
MY139056A (en) 2001-12-28 2009-08-28 Ab Enzymes Gmbh Microbially-expressed thermotolerant phytase for animal feed
US7943360B2 (en) 2002-04-19 2011-05-17 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
EP2258839B1 (en) 2004-06-21 2015-06-03 Novozymes A/S Proteases
AT501955B1 (de) * 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
US7816509B2 (en) * 2005-11-16 2010-10-19 Korean Research Institute Of Bioscience & Biotechnology Translational elongation factor promoter from Pichia pastoris and method for producing recombinant protein using the same
CN101460612B (zh) * 2006-04-04 2015-05-06 诺维信公司 肌醇六磷酸酶变体
EP2365064B1 (en) 2006-04-04 2014-12-24 Novozymes A/S Phytase variants
US8222386B2 (en) 2006-10-10 2012-07-17 Keck Graduate Institute P. pastoris ADH promoter and use thereof to direct expression of proteins
US8143023B2 (en) * 2007-01-26 2012-03-27 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in Pichia
JP2010524440A (ja) 2007-04-20 2010-07-22 ポリマン サイエンティフィック イミューンバイオロジッシュ フォーシュング ゲゼルシャフト ミット ベシュレンクテル ファフツング 発現系
WO2010004042A2 (en) * 2008-07-11 2010-01-14 Novozymes A/S Pichia pastoris das promoter variants
WO2010099153A2 (en) * 2009-02-25 2010-09-02 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
EP2689023A2 (en) 2011-03-22 2014-01-29 Merck Sharp & Dohme Corp. Promoters for high level recombinant expression in fungal host cells
CN102994501B (zh) 2012-10-15 2018-04-27 华南理工大学 具有组成型启动子活性的dna及其应用和巴斯德毕赤酵母表达载体
DK2964765T3 (da) 2013-03-08 2019-07-29 Keck Graduate Inst Of Applied Life Sciences Gærpromotorer fra pichia pastoris
DK3527667T3 (da) 2013-03-08 2021-02-22 Biogrammatics Inc Gærpromotorer til proteinekspression
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
EP2862933B8 (en) 2013-07-05 2019-12-11 bisy e.U. Bidirectional promoter
JP7278261B2 (ja) * 2017-08-23 2023-05-19 ノバルティス アーゲー マルチコピー遺伝子タンパク質発現系

Also Published As

Publication number Publication date
ES2716619T3 (es) 2019-06-13
PT2964775T (pt) 2019-05-06
EP3527667A2 (en) 2019-08-21
EP2964775A4 (en) 2017-03-01
EP3839055A2 (en) 2021-06-23
EP3839055A3 (en) 2021-10-20
US10676750B2 (en) 2020-06-09
US20230340507A1 (en) 2023-10-26
US20210032643A1 (en) 2021-02-04
EP3527667B1 (en) 2020-11-25
DK2964775T3 (da) 2019-04-15
HUE042897T2 (hu) 2019-07-29
EP3839055C0 (en) 2023-08-23
EP3527667A3 (en) 2019-10-02
EP2964775B1 (en) 2019-01-09
US20160024511A1 (en) 2016-01-28
US12264319B2 (en) 2025-04-01
EP3839055B1 (en) 2023-08-23
WO2014138679A1 (en) 2014-09-12
US11555194B2 (en) 2023-01-17
EP2964775A1 (en) 2016-01-13
CN105358706A (zh) 2016-02-24
CA2904333A1 (en) 2014-09-12
ES2961268T3 (es) 2024-03-11
CA2904333C (en) 2024-06-25
ES2856265T3 (es) 2021-09-27
BR112015021785A2 (pt) 2017-08-29

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