[go: up one dir, main page]

DK3382019T3 - Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri - Google Patents

Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri Download PDF

Info

Publication number
DK3382019T3
DK3382019T3 DK16868704.4T DK16868704T DK3382019T3 DK 3382019 T3 DK3382019 T3 DK 3382019T3 DK 16868704 T DK16868704 T DK 16868704T DK 3382019 T3 DK3382019 T3 DK 3382019T3
Authority
DK
Denmark
Prior art keywords
converting
nucleic acid
specific dna
acid base
molecular complex
Prior art date
Application number
DK16868704.4T
Other languages
English (en)
Inventor
Keiji Nishida
Zenpei Shimatani
Akihiko Kondo
Original Assignee
Univ Kobe Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kobe Nat Univ Corp filed Critical Univ Kobe Nat Univ Corp
Application granted granted Critical
Publication of DK3382019T3 publication Critical patent/DK3382019T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205Agrobacterium mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/78Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00Culture process characterised by temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y305/00Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005Cytidine deaminase (3.5.4.5)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK16868704.4T 2015-11-27 2016-11-25 Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri DK3382019T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2015232379 2015-11-27
JP2016134613 2016-07-06
PCT/JP2016/085075 WO2017090761A1 (ja) 2015-11-27 2016-11-25 標的化したdna配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体

Publications (1)

Publication Number Publication Date
DK3382019T3 true DK3382019T3 (da) 2022-05-30

Family

ID=58764366

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16868704.4T DK3382019T3 (da) 2015-11-27 2016-11-25 Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri

Country Status (8)

Country Link
US (1) US11220693B2 (da)
EP (1) EP3382019B1 (da)
JP (1) JP6923205B2 (da)
KR (1) KR102061438B1 (da)
CN (1) CN108495932B (da)
DK (1) DK3382019T3 (da)
ES (1) ES2914623T3 (da)
WO (1) WO2017090761A1 (da)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111471674A (zh) 2014-03-05 2020-07-31 国立大学法人神户大学 特异性转变靶向dna序列的核酸碱基的基因组序列的修饰方法、及其使用的分子复合体
JP6664693B2 (ja) * 2015-09-09 2020-03-13 国立大学法人神戸大学 標的化したdna配列の核酸塩基を特異的に変換する、グラム陽性菌のゲノム配列の変換方法、及びそれに用いる分子複合体
EP3382019B1 (en) 2015-11-27 2022-05-04 National University Corporation Kobe University Method for converting monocot plant genome sequence in which nucleic acid base in targeted dna sequence is specifically converted, and molecular complex used therein
SG11201809242VA (en) * 2016-04-21 2018-11-29 Univ Kobe Nat Univ Corp Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor
WO2017195905A1 (ja) 2016-05-13 2017-11-16 株式会社カネカ 形質転換植物の作製方法
WO2017195906A1 (ja) 2016-05-13 2017-11-16 株式会社カネカ 植物のゲノム編集方法
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
CN107043779B (zh) * 2016-12-01 2020-05-12 中国农业科学院作物科学研究所 一种CRISPR/nCas9介导的定点碱基替换在植物中的应用
WO2018143477A1 (ja) * 2017-02-06 2018-08-09 国立大学法人 筑波大学 双子葉植物のゲノムの改変方法
CN110157727A (zh) * 2017-12-21 2019-08-23 中国科学院遗传与发育生物学研究所 植物碱基编辑方法
CA3095291C (en) * 2018-03-26 2022-10-11 National University Corporation Kobe University Method for modifying target site in double-stranded dna in cell
WO2019213910A1 (en) * 2018-05-10 2019-11-14 Syngenta Participations Ag Methods and compositions for targeted editing of polynucleotides
JP7541508B2 (ja) * 2018-08-23 2024-08-28 サンガモ セラピューティクス, インコーポレイテッド 操作された標的特異的な塩基エディター
CN110607320B (zh) * 2018-11-23 2023-05-12 电子科技大学 一种植物基因组定向碱基编辑骨架载体及其应用
CN110257406B (zh) * 2019-06-21 2022-07-22 安徽省农业科学院水稻研究所 密码子植物化改造的Plant Nme2Cas9基因及其应用
WO2021046155A1 (en) 2019-09-03 2021-03-11 Voyager Therapeutics, Inc. Vectorized editing of nucleic acids to correct overt mutations
JP7616514B2 (ja) * 2019-09-10 2025-01-17 株式会社カネカ 腋芽メリステムを用いた植物の改変方法
EP4086353A4 (en) * 2020-01-03 2024-03-06 Kaneka Corporation METHOD FOR PLANT MODIFICATION
WO2022158561A1 (ja) * 2021-01-22 2022-07-28 国立大学法人東京大学 植物ゲノムの編集方法
CN117157399A (zh) * 2021-02-26 2023-12-01 国立大学法人神户大学 植物基因组进化工程半理性方法
US20240240196A1 (en) * 2021-05-26 2024-07-18 The Board Of Trustees Of The University Of Illinois C4 plants with increased photosynthetic efficiency
US20240279719A1 (en) * 2021-06-17 2024-08-22 The Board Of Trustees Of The University Of Illinois Crispr cascade

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003524368A (ja) 1997-08-26 2003-08-19 アリアド ジーン セラピューティクス インコーポレイテッド ニ量化ドメイン、三量化ドメインまたは四量化ドメインおよび補足的非相同転写活性化ドメイン、転写抑制ドメイン、dna結合ドメインまたはリガンド結合ドメインを含む融合蛋白
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
NZ579002A (en) 2007-03-02 2012-03-30 Danisco Cultures with improved phage resistance
WO2010132092A2 (en) 2009-05-12 2010-11-18 The Scripps Research Institute Cytidine deaminase fusions and related methods
US8772008B2 (en) 2009-05-18 2014-07-08 Sangamo Biosciences, Inc. Methods and compositions for increasing nuclease activity
US20110104787A1 (en) 2009-11-05 2011-05-05 President And Fellows Of Harvard College Fusion Peptides That Bind to and Modify Target Nucleic Acid Sequences
CA2783351C (en) 2009-12-10 2021-09-07 Regents Of The University Of Minnesota Tal effector-mediated dna modification
JP2013128413A (ja) 2010-03-11 2013-07-04 Kyushu Univ Pprモチーフを利用したrna結合性蛋白質の改変方法
WO2013058404A1 (ja) 2011-10-21 2013-04-25 国立大学法人九州大学 Pprモチーフを利用したrna結合性蛋白質の設計方法及びその利用
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
SG10201607943RA (en) 2012-03-23 2016-11-29 Cellectis Method to overcome dna chemical modifications sensitivity of engineered tale dna binding domains
FI3597749T3 (fi) 2012-05-25 2023-10-09 Univ California Menetelmiä ja koostumuksia rna-ohjattua kohde-dna-modifikaatiota varten ja rna-ohjattua transkription modulaatiota varten
WO2014071235A1 (en) 2012-11-01 2014-05-08 Massachusetts Institute Of Technology Genetic device for the controlled destruction of dna
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
PT2784162E (pt) 2012-12-12 2015-08-27 Broad Inst Inc Engenharia de sistemas, métodos e composições guia otimizadas para a manipulação de sequências
EP4234696A3 (en) * 2012-12-12 2023-09-06 The Broad Institute Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
WO2014165612A2 (en) 2013-04-05 2014-10-09 Dow Agrosciences Llc Methods and compositions for integration of an exogenous sequence within the genome of plants
US9873907B2 (en) * 2013-05-29 2018-01-23 Agilent Technologies, Inc. Method for fragmenting genomic DNA using CAS9
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
CN111471674A (zh) 2014-03-05 2020-07-31 国立大学法人神户大学 特异性转变靶向dna序列的核酸碱基的基因组序列的修饰方法、及其使用的分子复合体
EP4427809A3 (en) 2014-10-31 2024-12-04 The Trustees of The University of Pennsylvania Altering gene expression in car-t cells and uses thereof
EP3216867B1 (en) 2014-11-04 2020-04-15 National University Corporation Kobe University Method for modifying genome sequence to introduce specific mutation to targeted dna sequence by base-removal reaction, and molecular complex used therein
JP6664693B2 (ja) 2015-09-09 2020-03-13 国立大学法人神戸大学 標的化したdna配列の核酸塩基を特異的に変換する、グラム陽性菌のゲノム配列の変換方法、及びそれに用いる分子複合体
SG11201801809VA (en) 2015-09-09 2018-04-27 Univ Kobe Nat Univ Corp Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method
US12043852B2 (en) * 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
EP3382019B1 (en) 2015-11-27 2022-05-04 National University Corporation Kobe University Method for converting monocot plant genome sequence in which nucleic acid base in targeted dna sequence is specifically converted, and molecular complex used therein
SG11201809242VA (en) 2016-04-21 2018-11-29 Univ Kobe Nat Univ Corp Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor

Also Published As

Publication number Publication date
WO2017090761A1 (ja) 2017-06-01
KR102061438B1 (ko) 2019-12-31
EP3382019A1 (en) 2018-10-03
CN108495932B (zh) 2022-08-09
BR112018010681A2 (pt) 2018-11-13
EP3382019A4 (en) 2019-04-24
EP3382019B1 (en) 2022-05-04
ES2914623T3 (es) 2022-06-14
US20190085342A1 (en) 2019-03-21
BR112018010681A8 (pt) 2019-02-26
HK1256888A1 (en) 2019-10-04
JPWO2017090761A1 (ja) 2018-10-25
CN108495932A (zh) 2018-09-04
JP6923205B2 (ja) 2021-08-18
KR20180081811A (ko) 2018-07-17
US11220693B2 (en) 2022-01-11

Similar Documents

Publication Publication Date Title
DK3382019T3 (da) Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri
DK3348638T3 (da) Fremgangsmåde til at konvertere genomsekvens fra gram-positiv bakterie ved specifikt at konvertere nukleinsyrebase i tilsigtet dna-sekvens, og molekylkompleks anvendt dertil
DK3115457T3 (da) Fremgangsmåde til modificering af genomsekvens til specifikt at konvertere nukleinsyrebaser af målrettet dna-sekvens og molekylært kompleks til anvendelse i samme
DK3348636T3 (da) Fremgangsmåde til modificering af en genomsekvens, der specifikt omdanner nukleobasen af en targetteret DNA-sekvens, og molekylær sekvens anvendt i fremgangsmåden
DK3957744T3 (da) Reagenser og fremgangsmåder til molekylær stregkodning af enkeltcellenukleinsyrer
DK3216867T3 (da) Fremgangsmåde til at modificere genomsekvens for at introducere specifik mutation i tilsigtet dna-sekvens ved base-fjernelsesreaktion og molekylekompleks anvendt dertil
DK3310909T3 (da) Sammensætninger og fremgangsmåder til at føre proteiner til specifikke loci i genomet
DK3452591T5 (da) Makromolekyleanalyse under anvendelse af nukleinsyrekodning
DK3386550T3 (da) Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer
DK3250691T5 (da) Hybride crispr-dna/rna-polynucleotider og fremgangsmåder til deres anvendelse
DK3218511T3 (da) Fremgangsmåder og arrays til frembringelse og sekventering af monoklonale klynger af nukleinsyre
DK3456846T3 (da) Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre
DK3359671T3 (da) Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation
DK3302709T3 (da) Fremgangsmåder og sammensætninger til rna-guidet behandling af hiv-infektion
SG11201803646YA (en) Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
DK3325669T3 (da) Sammensætninger og fremgangsmåder til RNA-analyse
DK3381102T3 (da) Husenergianlæg og driftsfremgangsmåde til drift af et husenergianlæg
DK3362559T3 (da) Genetisk værktøj til transformation af clostridium-bakterier
DK3058092T3 (da) Fremgangsmåder og præparater til fremstilling af nukleinsyrebiblioteker
DK2912174T3 (da) Fremgangsmåde og materialer til isolering af nukleinsyrematerialer
DK3377653T3 (da) Fremgangsmåder til opnåelse og korrektion af biologisk sekvensinformation
DK3100026T3 (da) Fremgangsmåde, anordning og system til automatiseret bestemmelse af optiske tætheder eller ændring af optiske tætheder af reaktionsblandinger i omrørte reaktorer
DK3224374T3 (da) Fremgangsmåde til fragmentering af en nukleinsyre til sekvensering
DK3185694T3 (da) Hidtil ukendt fremgangsmåde til dyrkning af alger, især mikroalger
DK3350343T3 (da) Fremgangsmåder og sammensætninger til genommålberigelse og selektiv dna-sekventering