[go: up one dir, main page]

DK3344753T3 - Tre-dimensionelle hydrogeler til dyrkning af organoider - Google Patents

Tre-dimensionelle hydrogeler til dyrkning af organoider Download PDF

Info

Publication number
DK3344753T3
DK3344753T3 DK16760109.5T DK16760109T DK3344753T3 DK 3344753 T3 DK3344753 T3 DK 3344753T3 DK 16760109 T DK16760109 T DK 16760109T DK 3344753 T3 DK3344753 T3 DK 3344753T3
Authority
DK
Denmark
Prior art keywords
organoids
cultivating
dimensional hydrogels
hydrogels
dimensional
Prior art date
Application number
DK16760109.5T
Other languages
English (en)
Inventor
Matthias Lutolf
Nikolce Gjorevski
Original Assignee
Ecole Polytechnique Fed Lausanne Epfl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecole Polytechnique Fed Lausanne Epfl filed Critical Ecole Polytechnique Fed Lausanne Epfl
Application granted granted Critical
Publication of DK3344753T3 publication Critical patent/DK3344753T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0632—Cells of the oral mucosa
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2537/00—Supports and/or coatings for cell culture characterised by physical or chemical treatment
    • C12N2537/10—Cross-linking

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
DK16760109.5T 2015-09-03 2016-09-05 Tre-dimensionelle hydrogeler til dyrkning af organoider DK3344753T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/EP2015/070143 WO2017036533A1 (en) 2015-09-03 2015-09-03 Three-dimensional hydrogels for culturing adult epithelial stem cells and organoids
PCT/EP2016/070880 WO2017037295A1 (en) 2015-09-03 2016-09-05 Three dimensional hydrogels for culturing organoids

Publications (1)

Publication Number Publication Date
DK3344753T3 true DK3344753T3 (da) 2023-07-24

Family

ID=54064330

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16760109.5T DK3344753T3 (da) 2015-09-03 2016-09-05 Tre-dimensionelle hydrogeler til dyrkning af organoider

Country Status (10)

Country Link
US (1) US10934529B2 (da)
EP (1) EP3344753B1 (da)
JP (1) JP6867391B2 (da)
KR (1) KR20180038573A (da)
CA (1) CA2997375A1 (da)
DK (1) DK3344753T3 (da)
ES (1) ES2950502T3 (da)
HU (1) HUE062387T2 (da)
PT (1) PT3344753T (da)
WO (2) WO2017036533A1 (da)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
EP3149156B1 (en) 2014-05-28 2021-02-17 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
JP6804438B2 (ja) 2014-10-17 2020-12-23 チルドレンズ ホスピタル メディカル センター 多能性幹細胞を使用するヒト小腸のｉｎ ｖｉｖｏモデル、並びにそれを作製、及び使用する方法
EP4177335A1 (en) 2016-05-05 2023-05-10 Children's Hospital Medical Center Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same
US10073346B2 (en) 2016-07-08 2018-09-11 Cypre, Inc. Apparatus for patterning hydrogels into multi-well plates
EP3534976A4 (en) 2016-11-04 2020-09-16 Children's Hospital Medical Center PATHOLOGICAL MODELS OF HEPATIC ORGANOIDS AND ASSOCIATED METHODS OF MANUFACTURE AND USE
EP3548507A4 (en) 2016-12-05 2020-07-15 Children's Hospital Medical Center COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF
CA3055914A1 (en) * 2017-03-09 2018-09-13 Georgia Tech Research Corporation Synthetic hydrogel carriers for cellular structures, generation of organoids, and treatment of tissue injury
EP3609997A4 (en) 2017-04-14 2021-03-03 Children's Hospital Medical Center COMPOSITIONS OF STEM CELLS FROM MULTIPLE DONOR CELLS AND THEIR PREPARATION PROCEDURES
EP3630949A4 (en) * 2017-05-29 2021-03-10 Stemcell Technologies Canada Inc. COMPOSITIONS AND PROCESSES FOR OBTAINING ORGANOIDS
EP3694603A4 (en) 2017-10-10 2021-07-14 Children's Hospital Medical Center ESOPHAGUS TISSUE AND / OR ORGANOID COMPOSITIONS AND METHOD FOR MANUFACTURING THEREOF
US11744922B2 (en) * 2017-10-27 2023-09-05 Saint Louis University Biomimetic sponges for tissue regeneration
US11357887B2 (en) * 2017-11-10 2022-06-14 University Of Massachusetts Delivery systems based on hydrogel compositions and methods thereof
US12379372B2 (en) 2017-12-21 2025-08-05 Children's Hospital Medical Center Digitalized human organoids and methods of using same
CN108728399A (zh) * 2018-04-09 2018-11-02 浙江工商大学 基于小鼠不同区段小肠的体外类器官3d培养、传代、冻存、复苏和鉴定方法
CN108525021B (zh) * 2018-04-17 2021-05-18 山西医科大学 基于3d打印的含血管及毛囊结构组织工程皮肤及其制备方法
WO2019226120A1 (en) * 2018-05-23 2019-11-28 Agency For Science, Technology And Research A tumour cell culture system and a method of preparing a tumour cell culture system
EP3575391B1 (en) * 2018-06-01 2022-02-16 Cellendes GmbH Hydrogels for cultivating pancreatic organoids
KR102887406B1 (ko) 2018-07-26 2025-11-19 칠드런즈 호스피탈 메디칼 센터 간-담도-췌장 조직 및 이를 제조하는 방법
WO2020056158A1 (en) 2018-09-12 2020-03-19 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
KR20200065892A (ko) * 2018-11-30 2020-06-09 오가노이드사이언스 주식회사 오가노이드의 생체 이식용 조성물
WO2020139041A1 (ko) * 2018-12-28 2020-07-02 주식회사 씨아이에스티이엠 전신경화증 질환 모델 및 이의 용도
CN110305191A (zh) * 2019-07-11 2019-10-08 武汉科技大学 一种短肽、水凝胶及其用做伤口敷料的应用
US12084685B2 (en) 2019-07-29 2024-09-10 Massaachusetts Institute Of Technology Synthetic hydrogels for organogenesis
EP3789049A1 (en) * 2019-09-06 2021-03-10 QGel SA Method for obtaining healthy intestinal organoids
KR102228400B1 (ko) 2019-09-24 2021-03-16 고려대학교 산학협력단 뇌 오가노이드 제조 방법
BR112022010694A2 (pt) * 2019-12-04 2022-08-23 Precision Cancer Tech Inc Método e kit para crescimento celular
KR102291265B1 (ko) * 2020-03-19 2021-08-18 연세대학교 산학협력단 심장 오가노이드, 이의 제조 방법 및 이를 이용한 약물 독성 평가 방법
WO2022031857A2 (en) * 2020-08-05 2022-02-10 The Regents Of The University Of California Degradable hollow shell particles for high-throughput screening and sorting of cells
US11628382B2 (en) 2020-08-26 2023-04-18 Duke University Methods and apparatuses for purification of gel droplets supporting biological tissue
CA3190689A1 (en) 2020-08-31 2022-03-03 The Board Of Trustees Of University Of Illinois Alginate-coated mesenchymal stromal and progenitor cells and methods for using the same
CN112195152B (zh) * 2020-09-29 2023-04-07 南方医科大学南方医院 一种人结直肠癌组织类器官的培养方法及其应用
CN114874971B (zh) * 2021-02-05 2023-08-15 中国农业大学 一种细胞培养液及其在鸡小肠类器官培养中的应用
KR20230007766A (ko) * 2021-07-06 2023-01-13 서강대학교산학협력단 혈관화된 신장 오가노이드 형성을 위한 세포기질의 최적화 및 오가노이드 칩 개발
US20240376438A1 (en) * 2021-08-26 2024-11-14 Georgia Tech Research Corporation Hydrogels comprising cell adhesive peptides and methods of use thereof
WO2023076275A2 (en) * 2021-10-27 2023-05-04 Regenerative Research Foundation Cell culture feeding device
CN114214266B (zh) * 2021-11-18 2022-09-02 创芯国际生物科技(广州)有限公司 一种凝胶组合物、生物支架凝胶及其制备方法和应用
CN114573833B (zh) * 2021-12-28 2023-12-08 温州医科大学附属眼视光医院 一种用于细胞三维培养的peg-rgd多肽水凝胶材料及其制备方法与应用
CN115160761B (zh) * 2022-07-19 2024-05-28 杭州准星医学科技有限公司 微气泡凝胶的制备方法、微气泡凝胶基质胶制备方法及类器官的培养方法
WO2024025193A1 (ko) * 2022-07-25 2024-02-01 아주대학교산학협력단 기계적 강도가 조절된 기질을 포함하는 줄기세포 배양용 조성물 및 이의 용도
KR102882620B1 (ko) * 2022-11-23 2025-11-06 가천대학교 산학협력단 전립선 오가노이드를 이용한 전립선 질환 치료물질의 스크리닝 방법
WO2024216129A1 (en) * 2023-04-14 2024-10-17 Prohibix Llc Hyaluronic acid compositions with low extents of ester linkages and methods of use thereof
AU2024304677A1 (en) * 2023-06-14 2025-11-27 Genentech, Inc. Methods of producing tissue-derived epithelial organoids and uses thereof
WO2025014230A1 (ko) * 2023-07-07 2025-01-16 주식회사 히에라바이오 폐유래 줄기세포의 배양 방법 및 이의 용도
CN118028229B (zh) * 2024-04-11 2024-06-21 成都云测医学生物技术有限公司 一种从神经类器官获得间充质干细胞的制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1446453A1 (en) 2001-11-07 2004-08-18 Universität Zürich Synthetic matrix for controlled cell ingrowth and tissue regeneration and endometriosis
CA2691787A1 (en) * 2007-06-29 2009-01-08 The Trustees Of The University Of Pennsylvania Soft gel systems in modulating stem cell development
WO2009099555A2 (en) 2008-01-30 2009-08-13 Corning Incorporated Synthetic surfaces for culturing cells in chemically defined media
EP3061808B1 (en) * 2009-02-03 2020-08-12 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells
DK2455104T3 (da) * 2010-11-19 2013-10-14 Universitaetsklinikum Freiburg Biofunktionaliserede stimulusafhængige opløselige PEG-hydrogeler
US10465165B2 (en) 2013-01-28 2019-11-05 Rutgers, The State University Of New Jersey Growth matrices for stem cell propagation in vitro and in tissue regeneration
EP2801613A1 (en) * 2013-05-08 2014-11-12 Ecole Polytechnique Fédérale de Lausanne (EPFL) Arrays of discrete cell culture microenvironments, methods of making such arrays and uses thereof
US10195313B2 (en) 2014-04-10 2019-02-05 Wisconsin Alumni Research Foundation Method for forming hydrogel arrays using surfaces with differential wettability

Also Published As

Publication number Publication date
US20180258403A1 (en) 2018-09-13
JP6867391B2 (ja) 2021-04-28
CA2997375A1 (en) 2017-03-09
US10934529B2 (en) 2021-03-02
PT3344753T (pt) 2023-07-31
EP3344753B1 (en) 2023-04-26
KR20180038573A (ko) 2018-04-16
JP2018532421A (ja) 2018-11-08
EP3344753A1 (en) 2018-07-11
ES2950502T3 (es) 2023-10-10
WO2017037295A1 (en) 2017-03-09
HUE062387T2 (hu) 2023-11-28
WO2017036533A1 (en) 2017-03-09

Similar Documents

Publication Publication Date Title
DK3344753T3 (da) Tre-dimensionelle hydrogeler til dyrkning af organoider
IL261271A (en) Methods for making l-glufosinate
DK3481942T3 (da) Fremgangsmåder til dyrkning af organoider
EP3563977A4 (en) TOOL SYSTEM
DK3326452T3 (da) Dyrkningsopbevaringssystem
LT3347061T (lt) Įšvirkščiami makroporiniai hidrogeliai
EP3374114A4 (en) WORKBENCH
DK3072835T3 (da) Fremgangsmåde til fremføring
DK3294700T3 (da) Fremgangsmåde til fremstilling af limonen-4-ol
PL3316675T3 (pl) System uprawy
EP3333833A4 (en) TECHNOLOGY SIMULATOR
DK3186244T3 (da) Dioxolananaloger af uridin til behandling af cancer
FI20155008A7 (fi) Ajoneuvoavusteinen työlaite
DK3529233T3 (da) Fremgangsmåde til fremstilling af 3-alkylsulfanyl-2-chlor-n-(1-alkyl-1h-tetrazol-5-yl)-4-trifluoromethyl-benzamider
PL4235628T3 (pl) System tworzenia środowiska
PT3207164T (pt) Métodos de cultura semi-contínuos
DK3170627T3 (da) Redskab
DK3274143T3 (da) Fremgangsmåde til fremstilling af lignocellulosematerialer
DK3109141T3 (da) Holder
EP3498837A4 (en) Genome editing method
DK3206886T3 (da) Fremgangsmåde til at fremstille tredimensionelle strukturelle overflader
DK3231790T3 (da) Fremgangsmåde til fremstilling af kationisk lipid
ES2789757T8 (es) Herramienta de conformado
DK3244717T3 (da) Jordbearbejdningsredskab
BR112016027580A2 (pt) Formulação de ceritinib