DK3207134T3 - Kontiguitetsbevarende transposition - Google Patents
Kontiguitetsbevarende transposition Download PDFInfo
- Publication number
- DK3207134T3 DK3207134T3 DK15797490.8T DK15797490T DK3207134T3 DK 3207134 T3 DK3207134 T3 DK 3207134T3 DK 15797490 T DK15797490 T DK 15797490T DK 3207134 T3 DK3207134 T3 DK 3207134T3
- Authority
- DK
- Denmark
- Prior art keywords
- contiguity
- conversing
- transposition
- conversing transposition
- contiguity conversing
- Prior art date
Links
- 230000017105 transposition Effects 0.000 title 1
- XDTMQSROBMDMFD-UHFFFAOYSA-N C1CCCCC1 Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462065544P | 2014-10-17 | 2014-10-17 | |
| US201562157396P | 2015-05-05 | 2015-05-05 | |
| PCT/US2015/056040 WO2016061517A2 (en) | 2014-10-17 | 2015-10-16 | Contiguity preserving transposition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3207134T3 true DK3207134T3 (da) | 2019-09-23 |
Family
ID=54601997
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15797490.8T DK3207134T3 (da) | 2014-10-17 | 2015-10-16 | Kontiguitetsbevarende transposition |
Country Status (6)
| Country | Link |
|---|---|
| EP (2) | EP3207134B1 (da) |
| CN (2) | CN114045282B (da) |
| DK (1) | DK3207134T3 (da) |
| ES (1) | ES2745814T3 (da) |
| LT (1) | LT3207134T (da) |
| PT (1) | PT3207134T (da) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728477B (zh) * | 2017-04-24 | 2022-02-22 | 华东理工大学 | 一种高效的转座突变系统及构建方法 |
| WO2019195268A2 (en) | 2018-04-02 | 2019-10-10 | Grail, Inc. | Methylation markers and targeted methylation probe panels |
| AU2019262048B2 (en) * | 2018-05-03 | 2025-09-04 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| CN112739829A (zh) * | 2018-09-27 | 2021-04-30 | 深圳华大生命科学研究院 | 测序文库的构建方法和得到的测序文库及测序方法 |
| AU2019351130B2 (en) | 2018-09-27 | 2025-10-23 | GRAIL, Inc | Methylation markers and targeted methylation probe panel |
| BR112021006183A2 (pt) * | 2018-11-30 | 2021-06-29 | Illumina, Inc. | análise de múltiplos analitos com o uso de um único ensaio |
| US12385083B2 (en) | 2018-12-10 | 2025-08-12 | 10X Genomics, Inc. | Methods of using master / copy arrays for spatial detection |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| BR112021012755A2 (pt) * | 2019-07-12 | 2022-04-26 | Illumina Cambridge Ltd | Composições e métodos para preparar bibliotecas de sequenciamento de ácidos nucleicos usando crispr/cas9 imobilizadas em um suporte sólido |
| FI3931355T3 (fi) * | 2019-12-02 | 2023-04-26 | Illumina Cambridge Ltd | Genomisen dna:n amplifioitujen säilyneen vierekkäisyyden kirjastofragmenttien aikaperusteinen klusterikuvaus |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| IL294909A (en) | 2020-02-13 | 2022-09-01 | Zymergen Inc | Metagenomic library and natural product discovery platform |
| US20230174969A1 (en) * | 2020-05-18 | 2023-06-08 | Mgi Tech Co., Ltd. | Barcoded transposase complex and application thereof in high-throughput sequencing |
| AU2021366658A1 (en) * | 2020-10-21 | 2023-06-22 | Illumina Cambridge Limited | Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput |
| EP4121555A1 (en) | 2020-12-21 | 2023-01-25 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| CN112795563A (zh) * | 2021-03-23 | 2021-05-14 | 上海欣百诺生物科技有限公司 | 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法 |
| WO2022236054A1 (en) | 2021-05-06 | 2022-11-10 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| WO2023225519A1 (en) | 2022-05-17 | 2023-11-23 | 10X Genomics, Inc. | Modified transposons, compositions and uses thereof |
| CN115747301B (zh) * | 2022-08-01 | 2023-12-22 | 深圳赛陆医疗科技有限公司 | 一种测序文库的构建方法、构建测序文库的试剂盒及基因测序方法 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| CN101432439B (zh) * | 2006-02-24 | 2013-07-24 | 考利达基因组股份有限公司 | Dna阵列上的高通量基因组测序 |
| DK2376517T3 (da) * | 2008-10-24 | 2013-02-11 | Epict Technologies Corp | Transposon-ende-sammensætninger og fremgangsmåder til at modificere nukleinsyrer |
| US9029103B2 (en) | 2010-08-27 | 2015-05-12 | Illumina Cambridge Limited | Methods for sequencing polynucleotides |
| RU2013113407A (ru) * | 2010-08-27 | 2014-10-10 | Дженентек, Инк. | Способы улавливания и секвенирования нуклеиновых кислот |
| DK2625320T3 (da) * | 2010-10-08 | 2019-07-01 | Harvard College | High-throughput enkeltcellestregkodning |
| CA2821299C (en) * | 2010-11-05 | 2019-02-12 | Frank J. Steemers | Linking sequence reads using paired code tags |
| US8829171B2 (en) * | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
| CA2821559C (en) * | 2011-01-28 | 2017-01-31 | Illumina, Inc. | Oligonucleotide replacement for di-tagged and directional libraries |
| WO2012106546A2 (en) * | 2011-02-02 | 2012-08-09 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| WO2012108864A1 (en) | 2011-02-08 | 2012-08-16 | Illumina, Inc. | Selective enrichment of nucleic acids |
| NO2694769T3 (da) * | 2012-03-06 | 2018-03-03 | ||
| WO2013138536A1 (en) | 2012-03-13 | 2013-09-19 | Swift Biosciences, Inc. | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
| US9644199B2 (en) * | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
| US9683230B2 (en) * | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| WO2014136930A1 (ja) * | 2013-03-07 | 2014-09-12 | 積水メディカル株式会社 | メチル化ｄｎａの検出方法 |
| EP2970951B1 (en) * | 2013-03-13 | 2019-02-20 | Illumina, Inc. | Methods for nucleic acid sequencing |
| CN110964796B (zh) * | 2013-08-28 | 2024-04-05 | 贝克顿迪金森公司 | 大规模平行单细胞分析 |
| ES2912183T3 (es) * | 2013-12-30 | 2022-05-24 | Atreca Inc | Análisis de ácidos nucleicos asociados a células individuales utilizando códigos de barras de ácidos nucleicos |
-
2015
- 2015-10-16 PT PT15797490T patent/PT3207134T/pt unknown
- 2015-10-16 CN CN202111171107.9A patent/CN114045282B/zh active Active
- 2015-10-16 DK DK15797490.8T patent/DK3207134T3/da active
- 2015-10-16 EP EP15797490.8A patent/EP3207134B1/en active Active
- 2015-10-16 EP EP19183798.8A patent/EP3636757A1/en active Pending
- 2015-10-16 LT LTEP15797490.8T patent/LT3207134T/lt unknown
- 2015-10-16 CN CN201580068116.1A patent/CN107969137B/zh active Active
- 2015-10-16 ES ES15797490T patent/ES2745814T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107969137B (zh) | 2021-10-26 |
| ES2745814T3 (es) | 2020-03-03 |
| CN114045282A (zh) | 2022-02-15 |
| EP3207134B1 (en) | 2019-07-03 |
| EP3636757A1 (en) | 2020-04-15 |
| EP3207134A2 (en) | 2017-08-23 |
| CN114045282B (zh) | 2025-03-07 |
| LT3207134T (lt) | 2019-09-10 |
| CN107969137A (zh) | 2018-04-27 |
| PT3207134T (pt) | 2019-09-17 |
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