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DK3110947T3 - Diacylglycerolacyltransferase (dga1)-polynukleotider og fremgangsmåder til forøgelse af gærcellelipidfremstilling ved hjælp af overekspression af heterolog dga1 - Google Patents

Diacylglycerolacyltransferase (dga1)-polynukleotider og fremgangsmåder til forøgelse af gærcellelipidfremstilling ved hjælp af overekspression af heterolog dga1 Download PDF

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Publication number
DK3110947T3
DK3110947T3 DK15751487T DK15751487T DK3110947T3 DK 3110947 T3 DK3110947 T3 DK 3110947T3 DK 15751487 T DK15751487 T DK 15751487T DK 15751487 T DK15751487 T DK 15751487T DK 3110947 T3 DK3110947 T3 DK 3110947T3
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DK
Denmark
Prior art keywords
dga1
transpherase
glycerolacyl
heterologic
diacyl
Prior art date
Application number
DK15751487T
Other languages
English (en)
Inventor
Elena E Brevnova
Arthur J Shaw Iv
Emily H Greenhagen
Original Assignee
Novogy Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novogy Inc filed Critical Novogy Inc
Application granted granted Critical
Publication of DK3110947T3 publication Critical patent/DK3110947T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102Diacylglycerol O-acyltransferase (2.3.1.20)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK15751487T 2014-02-24 2015-02-24 Diacylglycerolacyltransferase (dga1)-polynukleotider og fremgangsmåder til forøgelse af gærcellelipidfremstilling ved hjælp af overekspression af heterolog dga1 DK3110947T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461943664P 2014-02-24 2014-02-24
PCT/US2015/017227 WO2015127421A1 (en) 2014-02-24 2015-02-24 Diacylglycerol acyl transferase (dga1) polynucleotides, and methods of increasing yeast cell lipid production by overexpression of heterologous dga1

Publications (1)

Publication Number Publication Date
DK3110947T3 true DK3110947T3 (da) 2019-11-04

Family

ID=53879132

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15751487T DK3110947T3 (da) 2014-02-24 2015-02-24 Diacylglycerolacyltransferase (dga1)-polynukleotider og fremgangsmåder til forøgelse af gærcellelipidfremstilling ved hjælp af overekspression af heterolog dga1

Country Status (6)

Country Link
US (3) US10260052B2 (da)
EP (1) EP3110947B1 (da)
CN (2) CN106574257B (da)
AU (2) AU2015218647B2 (da)
DK (1) DK3110947T3 (da)
WO (1) WO2015127421A1 (da)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015218647B2 (en) * 2014-02-24 2021-10-21 Ginkgo Bioworks, Inc. Diacylglycerol acyl transferase (DGA1) polynucleotides, and methods of increasing yeast cell lipid production by overexpression of heterologous DGA1
WO2015184303A1 (en) 2014-05-29 2015-12-03 Novogy, Inc. Increasing lipid production in oleaginous yeast
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
CN113337410A (zh) * 2021-06-04 2021-09-03 广西壮族自治区亚热带作物研究所(广西亚热带农产品加工研究所) 一种防治百香果黑斑病的药剂及其制备方法
CN116555362A (zh) * 2022-01-29 2023-08-08 罗盖特公司 使用解脂耶氏酵母生产脂质的方法
CN116396875A (zh) * 2022-12-20 2023-07-07 华东理工大学 一种解脂耶氏酵母基因工程菌及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6791008B1 (en) * 1999-11-12 2004-09-14 Scandinavian Biotechnology Research (Scanbi) Ab Use of a class of enzymes and their encoding genes to increase the oil content in transgenic organisms
KR20020026053A (ko) * 2000-09-30 2002-04-06 노승권 음료용 식물성 스테롤의 분산방법 및 이를 함유하는 음료
CA2492205A1 (en) * 2002-07-31 2004-02-05 Monsanto Technology, Llc Diacylglycerol acyltransferase nucleic acid sequences and associated products
US7550286B2 (en) * 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US7273746B2 (en) * 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
EP2730656A1 (en) * 2007-05-24 2014-05-14 E. I. du Pont de Nemours and Company Soybean meal from beans having DGAT genes from yarrowia lipolytica for increased seed storage lipid production and altered fatty acid profiles in soybean
EP2620501A3 (en) * 2008-05-23 2013-10-02 E. I. du Pont de Nemours and Company DGAT genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8502026B2 (en) * 2009-08-24 2013-08-06 Monsanto Technology Llc Transgenic plants with enhanced agronomic traits
BR112012011980A2 (pt) * 2009-11-19 2021-09-08 Oxyrane Uk Limited Métodos de produzir células transformadas de yarrowia lipolytica e proteínas-alvocompreendendo n-glicanos, células transformadas de yarrowia lipolytica e suas culturas, bem como composição compreendendo glicoproteínas
CA2804025C (en) * 2010-06-28 2023-02-21 Commonwealth Scientific And Industrial Research Organisation Methods of producing lipids
EP2714724A1 (en) 2011-05-26 2014-04-09 E. I. Du Pont de Nemours and Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
AU2015218647B2 (en) * 2014-02-24 2021-10-21 Ginkgo Bioworks, Inc. Diacylglycerol acyl transferase (DGA1) polynucleotides, and methods of increasing yeast cell lipid production by overexpression of heterologous DGA1

Also Published As

Publication number Publication date
CN113151319A (zh) 2021-07-23
US20160362663A1 (en) 2016-12-15
CN106574257A (zh) 2017-04-19
US20220380737A1 (en) 2022-12-01
AU2022200122A1 (en) 2022-02-10
US10260052B2 (en) 2019-04-16
EP3110947A1 (en) 2017-01-04
AU2015218647A1 (en) 2016-09-08
US20190316098A1 (en) 2019-10-17
US11352610B2 (en) 2022-06-07
EP3110947A4 (en) 2017-11-01
AU2015218647B2 (en) 2021-10-21
WO2015127421A1 (en) 2015-08-27
EP3110947B1 (en) 2019-09-04
CN106574257B (zh) 2021-06-04

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