[go: up one dir, main page]

DK2847324T3 - Produktion af rotaviruslignende partikler i planter - Google Patents

Produktion af rotaviruslignende partikler i planter Download PDF

Info

Publication number
DK2847324T3
DK2847324T3 DK13787880.7T DK13787880T DK2847324T3 DK 2847324 T3 DK2847324 T3 DK 2847324T3 DK 13787880 T DK13787880 T DK 13787880T DK 2847324 T3 DK2847324 T3 DK 2847324T3
Authority
DK
Denmark
Prior art keywords
plant
rotavirus
leu
asn
protein
Prior art date
Application number
DK13787880.7T
Other languages
English (en)
Inventor
David Levi Rutendo Mutepfa
Edward Peter Rybicki
Marc Andre Daoust
Nathalie Landry
Pierre Olivier Lavoie
Inga Isabel Hitzeroth
Masaaki Arai
Naomi Asahara
Original Assignee
Medicago Inc
Mitsubishi Tanabe Pharma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medicago Inc, Mitsubishi Tanabe Pharma Corp filed Critical Medicago Inc
Application granted granted Critical
Publication of DK2847324T3 publication Critical patent/DK2847324T3/da

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/15—Reoviridae, e.g. calf diarrhea virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12311—Rotavirus, e.g. rotavirus A
    • C12N2720/12334—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12311—Rotavirus, e.g. rotavirus A
    • C12N2720/12351—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (11)

  1. PRODUKTION AF ROTA VIRUSLIGNENDE PARTIKLER I PLANTER
    1. Fremgangsmåde til produktion af en rotaviruslignende partikel (RLP) i en plante, plantedel eller plantecelle omfattende: a) indføring af en første nukleinsyre omfattende en første regulerende region, som er aktiv i planten, operationelt koblet til en første nukleotidsekvens, der koder for et første rotavirus strukturprotein udvalgt fra en af VP2, VP6 og VP7, en anden nukleinsyre omfattende en anden regulerende region, som er aktiv i planten, operationelt koblet til en anden nukleotidsekvens, der koder for et andet rotavirus strukturprotein udvalgt fra en af VP2, VP6 and VP7 og en tredje nukleinsyre omfattende en tredje regulerende region, som er aktiv i planten, operationelt koblet til en tredje nukleotidsekvens, der koder for et tredje rotavirus strukturprotein udvalgt fra en af VP2, VP6 og VP7, i planten, plantedelen eller plantecellen, hvori den første, anden eller tredje nukleotidsekvens, der koder for VP7, omfatter et trunkeret nativt signalpeptid eller et ikke-nativ signalpeptid fra et plantepolypeptid, og hvori RLP omfatter VP2, VP6 og VP7; eller a) tilførsel til en plante, plantedel eller plantecelle omfattende en første nukleinsyre omfattende en første regulerende region, som er aktiv i planten, operationelt koblet til en første nukleotidsekvens, der koder for et første rotavirus strukturprotein, en anden nukleinsyre omfattende en anden regulerende region, som er aktiv i planten, operationelt koblet til en anden nukleotidsekvens, der koder for et andet rotavirus strukturprotein og en tredje nukleinsyre omfattende en tredje regulerende region, som er aktiv i planten, operationelt koblet til en tredje nukleotidsekvens, der koder for et tredje rotavirusstrukturprotein, i planten, plantedelen eller plantecellen; hvori det første rotavirusstrukturprotein er VP2, det andet rotavirusstrukturprotein er VP6 og det tredje rotavirusstrukturprotein er VP7, hvori VP7 omfatter et trunkeret nativt signalpeptid eller et ikke-nativ signalpeptid fra et plantepolypeptid. b) inkubering af planten, plantedelen eller plantecellen under betingelser, der tillader transitorisk ekspression af den første, anden og tredje nukleinsyre, hvorved RLP’en produceres, c) høst af planten, plantedelen eller plantecellen og d) ekstrahering og oprensning af RLP’eme fra planten, plantedelen eller plantecellen i tilstedeværelse af calcium, hvori koncentrationen af calcium er mellem 1 mM og 1000 mM.
  2. 2. Fremgangsmåde ifølge krav 1, hvori en fjerde nukleinsyre omfattende en fjerde regulerende region, som er aktiv i planten og operationelt koblet til en fjerde nukleotidsekvens, der koder for et fjerde rotavirusstrukturprotein indføres i planten, plantedelen eller plantecellen i trin a) og udtrykkes, når planten, plantedelen eller plantecellen inkuberes i trin b), hvori det fjerde rotavirusstrukturprotein er VP4.
  3. 3. Fremgangsmåde ifølge krav 1, hvori det første rotavirusstrukturprotein er VP2, det andet rotavirusstrukturprotein er VP6, og det trejde rotavirusstrukturprotein er VP7.
  4. 4. Fremgangsmåde ifølge krav 1, hvori planten, plantedelen eller plantecellen får tilført en fjerde nukleinsyre omfattende en fjerde regulerende region, som er aktiv i planten og operationelt koblet til en fjerde nukleotidsekvens, der koder for et fjerde rotavirusstrukturprotein, hvori det fjerde rotavirusstrukturprotein er VP4.
  5. 5. Fremgangsmåde ifølge et hvilket som helst af kravene 1-4, hvori den første, anden, tredje eller fjerde nukleotidsekvens eller en kombination deraf er operationelt koblet til en cowpea-mosaikvirus (CPMV)-regulerende region.
  6. 6. Fremgangsmåde ifølge et hvilket som helst af kravene 1-4, hvori nukleotidsekvensen, der koder for VP2 omfatter fra 80 % til 100 % identitet med en nukleotidsekvens defineret ved SEQ ID NO: 13, SEQ ID NO: 14, eller SEQ ID NO: 45, nukleotidsekvensen, der koder for VP6 omfatter fra 80 % til 100 % identitet med en nukleotidsekvens defineret ved SEQ ID NO: 17, SEQ ID NO: 18 eller SEQ ID NO:46, nukleotidsekvensen, der koder for VP7 omfatter fra 80 % til 100 % identitet med en nukleotidsekvens defineret ved SEQ ID NO: 19, 20, 48, 49, 52, 53, 54 eller 57, VP4 omfatter fra 80 % til 100 % identitet med en nukleotidsekvens defineret ved SEQ ID NO: 15, 16, 47, 50 eller 51.
  7. 7. Fremgangsmåde ifølge krav 1, hvori den første, anden og tredje nukleinsyre indføres i planten, plantedelen eller plantecellen ved agro-infiltration med et inokulum af Agrobacteria omfattende en blanding af den første, anden og tredje nukleinsyre i forholdet 1:1:1, hvori den første, anden og tredje nukleinsyre udtrykkes transitorisk i planten, og planten er Nicotiana benthamiana.
  8. 8. Fremgangsmåde ifølge krav 2, hvori den første, anden, tredje og fjerde nukleinsyre indføres i planten, plantedelen eller plantecellen ved agro-infiltration med et inokulum af Agrobacteria omfattende en blanding af den første, anden, tredje og fjerde nukleinsyre i forholdet 1:1:1:1, hvori den første, anden, tredje og fjerde nukleinsyre udtrykkes transitorisk i planten, og planten er Nicotiana benthamiana.
  9. 9. Fremgangsmåde ifølge et hvilket som helst af kravene 1-5, hvori nukleotidsekvensens kodon-benyttelse justeres til foretrukket human kodon-benyttelse, øget GC-indhold eller en kombination deraf.
  10. 10. Fremgangsmåde ifølge krav 1 eller 4, hvori den første, anden og tredje nukleotidsekvens er operationelt koblet til et translationsforstærkerelement.
  11. 11. Fremgangsmåde ifølge krav 1, hvori det ikke-native signalpeptid er et alfalfaprotein-disulfidisomerase (PDI)-signalpeptid.
DK13787880.7T 2012-05-11 2013-05-10 Produktion af rotaviruslignende partikler i planter DK2847324T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261646058P 2012-05-11 2012-05-11
PCT/CA2013/050364 WO2013166609A1 (en) 2012-05-11 2013-05-10 Rotavirus-like particle production in plants

Publications (1)

Publication Number Publication Date
DK2847324T3 true DK2847324T3 (da) 2018-02-12

Family

ID=49550032

Family Applications (2)

Application Number Title Priority Date Filing Date
DK19186469.3T DK3597757T3 (da) 2012-05-11 2013-05-10 Fremstilling af rotaviruslignende partikel i planter
DK13787880.7T DK2847324T3 (da) 2012-05-11 2013-05-10 Produktion af rotaviruslignende partikler i planter

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK19186469.3T DK3597757T3 (da) 2012-05-11 2013-05-10 Fremstilling af rotaviruslignende partikel i planter

Country Status (23)

Country Link
US (1) US20150216961A1 (da)
EP (3) EP2847324B1 (da)
JP (2) JP6435259B2 (da)
KR (1) KR102162118B1 (da)
CN (2) CN108570456A (da)
AU (2) AU2013258849B2 (da)
BR (1) BR112014027736A2 (da)
CA (2) CA2942279A1 (da)
DK (2) DK3597757T3 (da)
EA (1) EA032089B1 (da)
ES (2) ES2845698T3 (da)
HU (2) HUE052625T2 (da)
IL (1) IL235290B (da)
MX (2) MX356314B (da)
MY (1) MY183566A (da)
NO (1) NO2853853T3 (da)
PH (2) PH12014502373B1 (da)
PL (2) PL2847324T3 (da)
PT (2) PT3597757T (da)
SG (1) SG11201406996VA (da)
TR (1) TR201802091T4 (da)
TW (2) TWI705139B (da)
WO (1) WO2013166609A1 (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015128397A (ja) * 2014-01-08 2015-07-16 国立大学法人広島大学 亜リン酸デヒドロゲナーゼ遺伝子、および、当該遺伝子を用いた出芽酵母の選択的培養方法
EA201791683A1 (ru) 2015-01-23 2017-12-29 Медикаго Инк. Получение ротавирусоподобных частиц в растениях
CN112724207A (zh) * 2015-05-21 2021-04-30 厦门大学 截短的轮状病毒vp4蛋白及其用途
US10548970B2 (en) 2015-10-05 2020-02-04 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Human rotavirus G9P[6] strain and use as a vaccine
US11413346B2 (en) 2015-11-09 2022-08-16 Curevac Ag Rotavirus vaccines
MA44722A (fr) * 2016-04-19 2019-02-27 Csir Vlp d'orbivirus chimériques produites par des plantes
GB201617480D0 (en) * 2016-10-14 2016-11-30 University Of Cape Town Production of soluble HIV envelope trimers in planta
WO2020168424A1 (en) * 2019-02-19 2020-08-27 Medicago Inc. Rotavirus vp7 fusion proteins and rotavirus-like particles comprising them
CN112251461B (zh) * 2020-10-22 2022-08-23 植链(上海)生物科技有限公司 一种采用本氏烟作为生物反应器制备的轮状病毒疫苗及其制备方法和应用
MY205588A (en) * 2021-02-23 2024-10-28 Univ Malaya A process for producing pharmaceutical or other heterologous protein via agrobacterium-mediated transformation of mucuna bracteata
CN114875047A (zh) * 2022-05-27 2022-08-09 江苏三仪生物工程有限公司 一种优化的猪轮状病毒外衣壳蛋白vp4的重组表达及应用
CN117866858B (zh) * 2023-11-30 2024-06-21 吉林农业大学 一种表达猪轮状病毒抗原的重组植物乳杆菌及其应用

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5428147A (en) 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
EP0152295B1 (en) 1984-02-09 1991-03-20 Royal Children's Hospital Research Foundation Live attenuated human rotavirus vaccine and preparation thereof
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US4636385A (en) 1985-02-15 1987-01-13 The Wistar Institute Of Anatomy & Biology Vaccine, method for its preparation, and use thereof in vaccinating humans against rotavirus infection
US4704275A (en) 1985-08-26 1987-11-03 The United States Of America As Represented By The Department Of Health And Human Services Vaccine against rotavirus diseases
US4751080A (en) 1985-08-26 1988-06-14 The United States Of America As Represented By The Department Of Health And Human Services Vaccine against rotavirus diseases
US4962028A (en) 1986-07-09 1990-10-09 Dna Plant Technology Corporation Plant promotors
US4927628A (en) 1987-09-21 1990-05-22 The United States Of America As Represented By The Department Of Health And Human Services Vaccine against rotavirus diseases and method of preparing same
DE69130068T2 (de) 1990-11-16 1999-01-07 Children's Hospital Medical Center, Cincinnati, Ohio Humane rotativen, impfstoffe und methoden
AU6512299A (en) 1998-10-07 2000-04-26 Boyce Institute For Plant Research At Cornell University Gemini virus vectors for gene expression in plants
JP2000139473A (ja) * 1998-11-16 2000-05-23 Hokkaido Green Bio Kenkyusho:Kk ロタウイルス遺伝子を導入した遺伝子組換え植物、この遺伝子組換え植物に由来するロタウイルスのタンパク質及びこのロタウイルスのタンパク質に対する抗体
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
KR100422158B1 (ko) * 2000-02-12 2004-03-10 (주)익스플로젠 재조합 로타바이러스의 구성체 단백질의 생산방법 및 이방법으로 생산된 백신 조성물
AU3613001A (en) 2000-02-12 2001-08-20 In-Sik Chung Production method of recombinant rotavirus structural proteins and vaccine composition
FR2806087B1 (fr) * 2000-03-07 2002-10-18 Agronomique Inst Nat Rech Particules pseudovirales de rotavirus et leur utilisation pour vectoriser des proteines ou des acides nucleiques
JP4698144B2 (ja) 2003-07-31 2011-06-08 富士通セミコンダクター株式会社 半導体装置の製造方法
CN1624141A (zh) * 2003-12-03 2005-06-08 中国农业科学院生物技术研究所 提高轮状病毒vp7或vp4植物表达含量的方法及产品
CN100569939C (zh) * 2004-02-26 2009-12-16 中国人民解放军免疫学研究所 表达人源轮状病毒外壳蛋白的转基因植物产品及其预防婴幼儿腹泻的用途
AU2006309281B2 (en) * 2005-05-27 2011-11-03 University Of Central Florida Chloroplasts engineered to express pharmaceutical proteins
EP1885394A4 (en) * 2005-06-01 2009-10-21 Dow Global Technologies Inc PRODUCTION OF MULTIVALENT PARTICLES LIKE VIRUSES
CN1772902A (zh) * 2005-09-30 2006-05-17 中国农业大学 轮状病毒vp6蛋白的一种编码基因及其应用
WO2007081447A2 (en) * 2005-11-22 2007-07-19 Novartis Vaccines And Diagnostics, Inc. Norovirus and sapovirus antigens
CA2615372A1 (en) * 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
CN103122354B (zh) * 2007-11-27 2018-03-23 麦迪卡格公司 表达血凝素之转基因植物中生产的重组流感病毒样颗粒(vlp)
AU2009256397B2 (en) * 2008-05-29 2014-06-05 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Expression and assembly of human group C rotavirus-like particles and uses thereof
US8513397B2 (en) 2008-08-27 2013-08-20 The Arizona Board Of Regents For And On Behalf Of Arizona State University DNA replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
US20130295609A1 (en) * 2010-11-04 2013-11-07 Medicago Inc. Plant expression system

Also Published As

Publication number Publication date
CN108570456A (zh) 2018-09-25
HUE035753T2 (hu) 2018-08-28
TR201802091T4 (tr) 2018-03-21
JP6435259B2 (ja) 2018-12-05
JP2015517304A (ja) 2015-06-22
IL235290B (en) 2021-03-25
EP2847324A4 (en) 2016-03-23
HUE052625T2 (hu) 2021-05-28
AU2013258849B2 (en) 2018-12-13
SG11201406996VA (en) 2014-11-27
EP2847324B1 (en) 2018-01-03
EA201492014A1 (ru) 2015-02-27
EP3321358A3 (en) 2018-06-20
PH12014502373A1 (en) 2015-01-12
MY183566A (en) 2021-02-26
CN104284978B (zh) 2018-02-02
PT2847324T (pt) 2018-03-02
EA032089B1 (ru) 2019-04-30
ES2845698T3 (es) 2021-07-27
TW201825681A (zh) 2018-07-16
CA2872803A1 (en) 2013-11-14
HK1206059A1 (en) 2015-12-31
KR20150013713A (ko) 2015-02-05
US20150216961A1 (en) 2015-08-06
CN104284978A (zh) 2015-01-14
WO2013166609A1 (en) 2013-11-14
CA2872803C (en) 2016-10-11
TW201346028A (zh) 2013-11-16
IL235290A0 (en) 2014-12-31
NO2853853T3 (da) 2018-04-21
EP3321358A2 (en) 2018-05-16
DK3597757T3 (da) 2021-01-11
AU2019201694A1 (en) 2019-04-04
PL2847324T3 (pl) 2018-06-29
MX2014013671A (es) 2015-05-07
KR102162118B1 (ko) 2020-10-07
EP2847324A1 (en) 2015-03-18
CA2942279A1 (en) 2013-11-14
PH12014502373B1 (en) 2019-11-13
PT3597757T (pt) 2020-12-31
EP3597757A1 (en) 2020-01-22
PH12018501452A1 (en) 2019-03-11
TWI614339B (zh) 2018-02-11
MX2018006186A (es) 2020-11-12
BR112014027736A2 (pt) 2017-10-10
PL3597757T3 (pl) 2021-05-31
EP3597757B1 (en) 2020-10-28
ES2658987T3 (es) 2018-03-13
MX356314B (es) 2018-05-23
TWI705139B (zh) 2020-09-21
AU2013258849A1 (en) 2014-11-13
JP2018171058A (ja) 2018-11-08

Similar Documents

Publication Publication Date Title
DK2847324T3 (da) Produktion af rotaviruslignende partikler i planter
KR101963792B1 (ko) 식물에서 광견병 바이러스 유사 입자 생성
US20210393765A1 (en) Rotavirus vp7 fusion proteins and rotavirus-like particles comprising them
US20190345454A1 (en) Rotavirus-like particle production in plants
HK1260786A1 (en) Rotavirus-like particle production in plants
HK1206059B (en) Rotavirus-like particle production in plants