[go: up one dir, main page]

DK2345662T3 - Genetisk modificerede gærarter og fermenteringsprocesser anvendende genetisk modificeret gær - Google Patents

Genetisk modificerede gærarter og fermenteringsprocesser anvendende genetisk modificeret gær Download PDF

Info

Publication number
DK2345662T3
DK2345662T3 DK10183662.5T DK10183662T DK2345662T3 DK 2345662 T3 DK2345662 T3 DK 2345662T3 DK 10183662 T DK10183662 T DK 10183662T DK 2345662 T3 DK2345662 T3 DK 2345662T3
Authority
DK
Denmark
Prior art keywords
gene
xylose
plasmid
seq
dna
Prior art date
Application number
DK10183662.5T
Other languages
English (en)
Inventor
Vineet Rajgarhia
Kari Koivuranta
Merja Pentillä
Marja Ilmen
Pirkko Suominen
Aristos Aristidou
Chris Miller
Stacey Olson
Laura Ruohonen
Original Assignee
Cargill Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cargill Inc filed Critical Cargill Inc
Application granted granted Critical
Publication of DK2345662T3 publication Critical patent/DK2345662T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01009—D-Xylulose reductase (1.1.1.9), i.e. xylitol dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017—Xylulokinase (2.7.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02003—Phosphoglycerate kinase (2.7.2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01001—Pyruvate decarboxylase (4.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03002—UDP-glucose 4-epimerase (5.1.3.2), i.e. UDP-galactose 4-epimerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (14)

1. Genetisk modificeret gærcelle som overudtrykker en funktionel xylulokinase og har et funktionelt, exogent xyloseisomerase-gen integreret i dets genom, hvor det exogene xyloseisomerase-gen er mindst 90% homolog med SEQ. ID. NO. 162 eller som koder for et xyloseisomerase-enzym som er mindst 60% homolog med SEQ. ID. NO. 163 og har en identitetsscore på mindre end 95% sammenlignet med SEQ. ID. NO. 59 og en positivscore på mindre end 97% sammenlignet med SEQ. ID. NO. 59, og hvor xyloseisomerase-genet er funktionelt forbundet til promotor- og terminatorsekvenser som er funktionelle i gærcellen, og hvor den modificerede gærcelle yderligere har en deletion eller afbrydelse af et nativt gen som producerer et enzym som katalyserer omdannelsen af xylose til xylitol.
2. Den genetisk modificerede gærcelle ifølge krav 1, som indeholder et funktionelt, exogent xylulokinase-gen funktionelt forbundet til promotor- og terminatorsekvenser som er funktionelle i gærcellen.
3. Den genetisk modificerede gærcelle ifølge krav 2, hvor det funktionelle, exogene xylulokinase-gen er et S. cerevisiae xylulokinase-gen.
4. Den genetisk modificerede gærcelle ifølge et hvilket som helst af kravene 1-3, hvor det native gen som producerer et enzym som katalyserer omdannelsen af xylose til xylitol er et funktionelt xylosereduktase-gen.
5. Den genetisk modificerede gærcelle ifølge et hvilket som helst af de foregående krav, som yderligere har en deletion eller afbrydelse af et funktionelt, nativt xylitoldehydrogenase-gen.
6. Den genetisk modificerede gærcelle ifølge et hvilket som helst af kravene 1-5, som er af slægten Kluyveromyces, Candida, Pichia, Brettanomyces, Pachysolen eller Saccharomyces.
7. Den genetisk modificerede gærcelle ifølge krav 6, som er af slægten Kluyveromyces eller Candida.
8. Den genetisk modificerede gærcelle ifølge et hvilket som helst af kravene 1-7, hvor xyloseisomerase-genet koder for et xyloseisomerase-enzym, som er mindst 90% homolog med SEQ. ID. NO. 163.
9. Fremgangsmåde til fermentering, ved hvilken en celle ifølge et hvilket som helst af kravene 1-8 dyrkes under fermenteringsbetingelser i en fermenteringssuppe som inkluderer et pentose-sukker.
10. Fremgangsmåden til fermentering ifølge krav 9, hvor ethanol produceres som et betydeligt fermenteringsprodukt.
11. Fremgangsmåden til fermentering ifølge krav 9, hvor cellen yderligere har integreret i dets genom et funktionelt, exogent lactatdehydrogenase-gen funktionelt forbundet til promotor- og terminatorsekvenser som er funktionelle i gærcellen, og lactat produceres som et betydeligt fermenteringsprodukt.
12. Fremgangsmåden til fermentering ifølge et hvilket som helst af kravene 9-11, i hvilken pentose-sukkeret inkluderer xylose.
13. Fremgangsmåden til fermentering ifølge et hvilket som helst af kravene 9-12, i hvilken fermenteringssuppen yderligere inkluderer et hexose-sukker.
14. Fremgangsmåden til fermentering ifølge et hvilket som helst af kravene 9-13, som er en anaerob fermentering.
DK10183662.5T 2003-05-02 2004-05-03 Genetisk modificerede gærarter og fermenteringsprocesser anvendende genetisk modificeret gær DK2345662T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46772703P 2003-05-02 2003-05-02
EP04751126A EP1626979B1 (en) 2003-05-02 2004-05-03 Genetically modified yeast species and fermentation processes using genetically modified yeast

Publications (1)

Publication Number Publication Date
DK2345662T3 true DK2345662T3 (da) 2016-08-15

Family

ID=33435110

Family Applications (4)

Application Number Title Priority Date Filing Date
DK04751126.6T DK1626979T3 (da) 2003-05-02 2004-05-03 Genetisk modificeret gærart og fremgangsmåder til fermentering, hvor den genetisk modificerede gær anvendes
DK10183744.1T DK2338895T3 (da) 2003-05-02 2004-05-03 Genetisk modificerede gærspecies og fermenteringsproces under anvendelse af genetisk modificeret gær
DK10183712.8T DK2301949T3 (da) 2003-05-02 2004-05-03 Genetisk modificerede gærarter og fermenteringsfremgangsmåder under anvendelse af genetisk modificeret gær
DK10183662.5T DK2345662T3 (da) 2003-05-02 2004-05-03 Genetisk modificerede gærarter og fermenteringsprocesser anvendende genetisk modificeret gær

Family Applications Before (3)

Application Number Title Priority Date Filing Date
DK04751126.6T DK1626979T3 (da) 2003-05-02 2004-05-03 Genetisk modificeret gærart og fremgangsmåder til fermentering, hvor den genetisk modificerede gær anvendes
DK10183744.1T DK2338895T3 (da) 2003-05-02 2004-05-03 Genetisk modificerede gærspecies og fermenteringsproces under anvendelse af genetisk modificeret gær
DK10183712.8T DK2301949T3 (da) 2003-05-02 2004-05-03 Genetisk modificerede gærarter og fermenteringsfremgangsmåder under anvendelse af genetisk modificeret gær

Country Status (12)

Country Link
US (5) US7943366B2 (da)
EP (4) EP2338895B1 (da)
JP (1) JP4711955B2 (da)
CN (2) CN1922198B (da)
AU (1) AU2004236724B2 (da)
BR (1) BRPI0409954B1 (da)
CA (1) CA2524387C (da)
DK (4) DK1626979T3 (da)
ES (4) ES2543385T3 (da)
PL (4) PL1626979T3 (da)
WO (1) WO2004099381A2 (da)
ZA (1) ZA200509697B (da)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1294728B1 (it) 1997-09-12 1999-04-12 Biopolo S C A R L Ceppi di lievito per la riproduzione di acido lattico
US7537826B2 (en) 1999-06-22 2009-05-26 Xyleco, Inc. Cellulosic and lignocellulosic materials and compositions and composites made therefrom
BR0306740A (pt) 2002-01-23 2004-12-28 Royal Nedalco B V Célula hospedeira transformada com um construto de ácido nucléico, molécula de ácido nucleico isolada, e, processos para a produção de etanol, e de um produto de fermentação
CN101914462B (zh) * 2004-07-16 2013-04-24 Dsm知识产权资产有限公司 发酵木糖的真核细胞的代谢工程
US7708214B2 (en) 2005-08-24 2010-05-04 Xyleco, Inc. Fibrous materials and composites
US20150328347A1 (en) 2005-03-24 2015-11-19 Xyleco, Inc. Fibrous materials and composites
JP2008545429A (ja) 2005-06-02 2008-12-18 カーギル インコーポレイテッド 遺伝子操作されたＩｓｓａｔｃｈｅｎｋｉａｏｒｉｅｎｔａｌｉｓ種又はその近隣種の酵母及びこの酵母を用いた発酵方法
CA2623751A1 (en) 2005-09-22 2007-04-05 Tate & Lyle Ingredients Americas, Inc. Improved strains for the production of organic acids
JP5121070B2 (ja) 2005-11-23 2013-01-16 ネイチャーワークス・エル・エル・シー Ｌ−又はｄ−ラクタート：フェリチトクロームｃ酸化還元酵素遺伝子が機能しない乳酸産生酵母
CN101442910A (zh) 2006-03-13 2009-05-27 卡吉尔公司 具有从磷酸二羟丙酮到甘油的途径被破坏的酵母细胞
US20080090283A1 (en) * 2006-10-13 2008-04-17 Rowan Universtity Ethanol resistant and furfural resistant strains of E. coli FBR5 for production of ethanol from cellulosic biomass
CN101463328B (zh) * 2007-12-19 2011-06-29 中国科学院微生物研究所 一株利用木糖发酵产乙醇的代谢工程酵母菌
US8455239B2 (en) * 2007-12-23 2013-06-04 Gevo, Inc. Yeast organism producing isobutanol at a high yield
AU2008345126A1 (en) 2007-12-27 2009-07-09 Gevo, Inc. Recovery of higher alcohols from dilute aqueous solutions
US8440449B2 (en) * 2008-09-30 2013-05-14 The United States Of America, As Represented By The Secretary Of Agriculture Transformed Saccharomyces cerevisiae engineered for xylose utilization
CA2744495C (en) * 2008-11-21 2017-05-30 Mascoma Corporation Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose
GB0822937D0 (en) 2008-12-16 2009-01-21 Terranol As Microorganism
WO2010074577A1 (en) 2008-12-24 2010-07-01 Royal Nedalco B.V. Xylose isomerase genes and their use in fermentation of pentose sugars
US20100311137A1 (en) 2009-06-08 2010-12-09 Ut-Battelle, Llc Microorganisms Having Enhanced Tolerance To Inhibitors and Stress
EP2446043A4 (en) * 2009-06-22 2013-02-13 Gevo Inc YEAST ORGANISMS FOR THE MANUFACTURE OF ISOBUTANOL
IN2012DN00568A (da) 2009-07-09 2015-06-12 Verdzyne Inc
WO2011078262A1 (ja) 2009-12-22 2011-06-30 株式会社豊田中央研究所 キシロースイソメラーゼ及びその利用
DK2546340T3 (da) * 2010-02-09 2016-06-27 Univ Yamaguchi Højekspressionspromotor, som stammer fra kluyveromyces marxianus
EA201201445A1 (ru) * 2010-04-21 2013-04-30 ДСМ АйПи АССЕТС Б.В. Клетка для ферментации смеси сахаров
WO2011149353A1 (en) 2010-05-27 2011-12-01 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
EP2576762A4 (en) 2010-06-03 2013-12-04 Mascoma Corp YEAST FOR THE EXPRESSION OF SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOVERIC PROCESSING USING STARCH AND CELLULOSE
CN103180451B (zh) * 2010-11-11 2014-12-31 丰田自动车株式会社 使用重组酵母的乙醇的制造方法
PL2663645T3 (pl) 2010-11-18 2015-05-29 Dsm Ip Assets Bv Szczepy drożdży zmodyfikowane do wytwarzania etanolu z glicerolu
BR112013012130A2 (pt) 2010-11-22 2017-10-31 Cargill Inc composições e métodos para titulação aumentada de etanol a partir de biomassa
EP2668281A4 (en) 2011-01-25 2015-08-26 Cargill Inc COMPOSITIONS AND METHODS OF SUCCINATE MANUFACTURE
CN102174549B (zh) 2011-02-22 2012-10-10 山东大学 一种编码木糖异构酶的核酸分子及其编码的木糖异构酶
WO2012125027A1 (en) 2011-03-14 2012-09-20 Dsm Ip Assets B.V. Yeast strains that ferment uronic acids
DK2697252T3 (da) * 2011-04-11 2016-12-12 Cargill Inc Sammensætninger og fremgangsmåder til forøget ethanol produktion fra biomasse
EP2546336A1 (en) 2011-07-11 2013-01-16 DSM IP Assets B.V. Yeast strains that consume uronic acids and produce fermentation products such as ethanol
CN102304541A (zh) * 2011-08-17 2012-01-04 中国药科大学 一种含组成型启动子pPGK和G418抗性基因的酿酒酵母表达载体及其构建方法
MY169074A (en) 2011-11-30 2019-02-13 Dsm Ip Assets Bv Yeast strains engineered to produce ethanol from acetic acid and glycerol
RU2595387C2 (ru) 2012-01-13 2016-08-27 Торэй Индастриз, Инк. Способ получения химического вещества
RU2595386C2 (ru) 2012-01-13 2016-08-27 Торэй Индастриз, Инк. Способ получения химического вещества
CN104203005A (zh) 2012-01-23 2014-12-10 帝斯曼知识产权资产管理有限公司 二萜的生产
CN102719481B (zh) * 2012-06-14 2014-11-05 中国科学院微生物研究所 一种将多个基因同时导入微生物基因组的方法
WO2014003439A1 (ko) * 2012-06-26 2014-01-03 한국생명공학연구원 에탄올 생산 경로가 봉쇄된 클루이베로마이세스 막시아누스 균주 및 이의 용도
BR112015001601A2 (pt) 2012-07-25 2017-11-07 Bioamber Sas células de levedura tendo curso de tca redutor de piruvato em succinato e superexpressão de uma enzima transidrogenase nad(p)+ exógena
IN2015DN01041A (da) 2012-08-28 2015-06-26 Dsm Ip Assets Bv
IN2015DN01042A (da) 2012-08-28 2015-06-26 Dsm Ip Assets Bv
BR112015004513A2 (pt) 2012-08-29 2017-11-21 Lallemand Hungary Liquidity Man Llc expressão de enzimas em levedura para cbp de oligômero derivado de lignocelulose
US8748152B1 (en) 2012-09-14 2014-06-10 The United States Of America, As Represented By The Secretary Of Agriculture Prevotella ruminicola xylose isomerase and co-expression with xylulokinase in yeast for xylose fermentation
US20140178954A1 (en) 2012-12-20 2014-06-26 E I Du Pont De Nemours And Company Expression of xylose isomerase activity in yeast
US9187743B2 (en) 2013-03-11 2015-11-17 E I Du Pont De Nemours And Company Bacterial xylose isomerases active in yeast cells
US8669076B1 (en) 2013-03-11 2014-03-11 E I Du Pont De Nemours And Company Cow rumen xylose isomerases active in yeast cells
JP6027559B2 (ja) 2013-03-28 2016-11-16 株式会社豊田中央研究所 キシロースイソメラーゼ活性を有するタンパク質及びその利用
TW201437367A (zh) 2013-03-29 2014-10-01 Far Eastern New Century Corp 可利用五碳糖及六碳糖生產乳酸之酵母菌
FI20135333L (fi) * 2013-04-05 2014-10-06 Teknologian Tutkimuskeskus Vtt Oy Hapon / happojen ja alkoholin tuotto sokereista käyttämällä hiivaa
MX367033B (es) 2013-05-31 2019-08-02 Dsm Ip Assets Bv Microorganismos para la produccion de diterpeno.
CN108064135A (zh) 2013-07-15 2018-05-22 帝斯曼知识产权资产管理有限公司 二萜的生产
WO2015014959A1 (en) 2013-07-31 2015-02-05 Dsm Ip Assets B.V. Recovery of steviol glycosides
CN104419716B (zh) * 2013-08-27 2017-12-29 天津大学 一种标准化、高精度、通用的功能模块构建方法
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
CN103421698B (zh) * 2013-09-05 2015-04-22 中国科学技术大学 木糖醇高温高产工程菌株的构建及应用
US20180030482A1 (en) 2014-04-18 2018-02-01 Moralco B.V. Use of acetaldehyde in the fermentative production of ethanol
BR102014027233A2 (pt) * 2014-10-30 2016-05-24 Biocelere Agroindustrial Ltda cassete de expressão, micro-organismo geneticamente modificado para expressão de xilose isomerase, processo para produção de biocombustíveis e/ou bioquímicos e biocombustível e/ou bioquímicos produzidos
TWI551688B (zh) * 2015-02-04 2016-10-01 遠東新世紀股份有限公司 發酵纖維素水解液來生成乙醇的方法
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
WO2016151046A1 (en) 2015-03-23 2016-09-29 Dsm Ip Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
EP3718417B1 (en) 2015-04-03 2025-09-03 DSM IP Assets B.V. Steviol glycosides
EP3286302A1 (en) 2015-04-21 2018-02-28 DSM IP Assets B.V. Geranylgeranyl pyrophosphate synthase
US11066688B2 (en) 2015-07-10 2021-07-20 Dsm Ip Assets B.V. Method for preparing a steviol glycoside composition
BR112018000690A2 (pt) 2015-07-13 2018-09-18 MARA Renewables Corporation micro-organismo recombinante, e, métodos para preparar um micro-organismo de metabolização de xilose e para produzir óleo.
BR112018002284A2 (pt) 2015-08-05 2018-12-11 Cargill Inc método de fermentação para produzir um bioproduto, levedura crabtree negativa geneticamente engenheirada e método para reduzir a produção de glicerol por uma levedura engenheirada
US20180230505A1 (en) 2015-08-13 2018-08-16 Dsm Ip Assets B.V. Steviol glycoside transport
WO2017060318A2 (en) 2015-10-05 2017-04-13 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
EP3380626B1 (en) 2015-11-24 2020-10-21 Cargill, Incorporated Genetically modified yeasts and fermentation processes using genetically modified yeasts
EP3390640A4 (en) * 2015-12-17 2019-05-22 Evonik Degussa (China) Co., Ltd GENETASSETTE FOR KNOCK-OUT THROUGH HOMOLOGOUS RECOMBINATION IN HEF-CELLS
US20190112590A1 (en) 2016-04-08 2019-04-18 Ei Du Pont De Nemours And Company Arabinose isomerases for yeast
CN109563117A (zh) 2016-08-09 2019-04-02 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷的结晶
WO2018029272A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
JP2019532655A (ja) 2016-10-27 2019-11-14 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． ゲラニルゲラニルピロリン酸シンターゼ
US11104886B2 (en) 2016-12-08 2021-08-31 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
CA3047840A1 (en) 2016-12-21 2018-06-28 Creatus Biosciences Inc. Method and organism expressing metschnikowia xylose transporters for increased xylose uptake
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
EP4155400A1 (en) 2017-06-27 2023-03-29 DSM IP Assets B.V. Udp-glycosyltransferases
US11718820B2 (en) 2017-08-17 2023-08-08 Cargill, Incorporated Genetically modified haploid Issatchenkia orientalis
US12215325B2 (en) 2018-03-27 2025-02-04 Basf Se Xylose metabolizing yeast
US11608364B2 (en) 2018-04-30 2023-03-21 Dsm Ip Assets B.V. Steviol glycoside transport
CA3187165A1 (en) 2020-06-15 2021-12-23 Melio Peptide Systems Inc. Microorganisms and methods for reducing bacterial contamination
WO2022084482A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
CN116179587A (zh) * 2022-09-07 2023-05-30 四川大学华西第二医院 一种在马克斯克鲁维酵母菌株中表达外源基因的重组载体
CN115851806A (zh) * 2022-09-07 2023-03-28 四川大学华西第二医院 一种在马克斯克鲁维酵母菌株中表达外源基因的重组载体

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL39710A (en) 1972-06-19 1975-04-25 Imi Inst For Res & Dev Recovery of acids from aqueous solutions by solvent extraction
US4771001A (en) 1986-03-27 1988-09-13 Neurex Corp. Production of lactic acid by continuous fermentation using an inexpensive raw material and a simplified method of lactic acid purification
US5866382A (en) 1990-04-06 1999-02-02 Xyrofin Oy Xylose utilization by recombinant yeasts
US5210296A (en) 1990-11-19 1993-05-11 E. I. Du Pont De Nemours And Company Recovery of lactate esters and lactic acid from fermentation broth
US5132456A (en) 1991-05-07 1992-07-21 The Regents Of The University Of California Sorption of carboxylic acid from carboxylic salt solutions at PHS close to or above the pKa of the acid, with regeneration with an aqueous solution of ammonia or low-molecular-weight alkylamine
FI913197A0 (fi) * 1991-07-01 1991-07-01 Xyrofin Oy Nya jaeststammar med reducerad foermaoga att metabolisera xylitol, foerfarande foer bildande av dessa och deras anvaendning vid framstaellning av xylitol.
US5420304A (en) 1992-03-19 1995-05-30 Biopak Technology, Ltd. Method to produce cyclic esters
AT398982B (de) 1993-02-18 1995-02-27 Vogelbusch Gmbh Verfahren zur abtrennung und reinigung von milchsäure
US5510526A (en) 1993-06-29 1996-04-23 Cargill, Incorporated Lactic acid production, separation and/or recovery process
US5789210A (en) 1993-11-08 1998-08-04 Purdue Research Foundation Recombinant yeasts for effective fermentation of glucose and xylose
FR2712147B1 (fr) 1993-11-08 1996-02-02 Agronomique Inst Nat Rech Produit alimentaire, obtention et application à la fabrication de jus de fruits ou de vin.
IL109003A (en) 1994-03-16 1999-09-22 Yissum Res Dev Co Process and extractant composition for extracting water-soluble carboxylic and mineral acids
IN191596B (da) 1996-05-06 2003-12-06 Purdue Research Foundation
US6717014B1 (en) * 1996-06-28 2004-04-06 Fmc Corporation Processes for preparing haloamines and tertiary aminoalkylorganometallic compounds
IT1294728B1 (it) * 1997-09-12 1999-04-12 Biopolo S C A R L Ceppi di lievito per la riproduzione di acido lattico
AU3950799A (en) * 1998-04-20 1999-11-08 Forskarpatent I Syd Ab Genetically engineered yeast and mutants thereof for the efficient fermentation of lignocellulose hydrolysates
SE9901298D0 (sv) * 1999-04-09 1999-04-09 Forskarpatent I Syd Ab Xylose isomerase with improved kinetic properties
HUP0201204A3 (en) 1999-05-21 2004-07-28 Cargill Dow Llc Minnetonka Methods and materials for synthesis of organic products
US6762339B1 (en) 1999-05-21 2004-07-13 3M Innovative Properties Company Hydrophilic polypropylene fibers having antimicrobial activity
AU2001282174A1 (en) * 2000-07-13 2002-01-30 Danisco Sweeteners Oy Method for the production of xylitol
CA2430270A1 (en) 2000-11-22 2002-05-30 Cargill Dow Polymers, Llc Methods and materials for the synthesis of organic products
WO2003049525A2 (en) 2001-11-23 2003-06-19 Cargill Dow Llc Methods and materials for the production of organic products in cells of $i(candida) species
BR0306740A (pt) 2002-01-23 2004-12-28 Royal Nedalco B V Célula hospedeira transformada com um construto de ácido nucléico, molécula de ácido nucleico isolada, e, processos para a produção de etanol, e de um produto de fermentação
UA80976C2 (en) 2002-05-30 2007-11-26 Cargill Dow Llc Processes and materials for production of d-lactic acid in yeast
SE0302421D0 (sv) * 2003-09-11 2003-09-11 Forskarpatent I Syd Ab Construction of new xylose utilizing Saccharomyces cerevisiae strain
JP2008545429A (ja) * 2005-06-02 2008-12-18 カーギル インコーポレイテッド 遺伝子操作されたＩｓｓａｔｃｈｅｎｋｉａｏｒｉｅｎｔａｌｉｓ種又はその近隣種の酵母及びこの酵母を用いた発酵方法
JP5121070B2 (ja) * 2005-11-23 2013-01-16 ネイチャーワークス・エル・エル・シー Ｌ−又はｄ−ラクタート：フェリチトクロームｃ酸化還元酵素遺伝子が機能しない乳酸産生酵母
JP5219074B2 (ja) * 2008-01-24 2013-06-26 独立行政法人産業技術総合研究所 キシロース発酵能が優れた六炭糖・五炭糖同時発酵酵母およびそれを用いたエタノールの高効率生産方法
WO2011019894A1 (en) * 2009-08-12 2011-02-17 Gevo, Inc. Cytosolic isobutanol pathway localization for the production of isobutanol

Also Published As

Publication number Publication date
EP2301949B1 (en) 2015-06-24
JP4711955B2 (ja) 2011-06-29
DK2338895T3 (da) 2013-09-08
CN1922198B (zh) 2012-07-11
AU2004236724B2 (en) 2008-07-10
CN1922198A (zh) 2007-02-28
DK2301949T3 (da) 2015-07-20
CA2524387A1 (en) 2004-11-18
DK1626979T3 (da) 2012-07-09
WO2004099381A2 (en) 2004-11-18
US8623633B2 (en) 2014-01-07
ES2385561T3 (es) 2012-07-26
US20160340697A1 (en) 2016-11-24
EP2338895A1 (en) 2011-06-29
ES2426966T3 (es) 2013-10-28
EP2301949A1 (en) 2011-03-30
EP1626979A4 (en) 2006-11-15
US20110287505A1 (en) 2011-11-24
AU2004236724A1 (en) 2004-11-18
US9410158B2 (en) 2016-08-09
EP1626979B1 (en) 2012-06-06
ES2543385T3 (es) 2015-08-18
CN102703336A (zh) 2012-10-03
CA2524387C (en) 2012-12-04
PL2338895T3 (pl) 2013-11-29
BRPI0409954B1 (pt) 2016-07-12
ES2586618T3 (es) 2016-10-17
ZA200509697B (en) 2007-03-28
EP1626979A2 (en) 2006-02-22
US20140080192A1 (en) 2014-03-20
JP2006525029A (ja) 2006-11-09
US20110287506A1 (en) 2011-11-24
EP2345662B1 (en) 2016-06-22
PL2345662T3 (pl) 2016-11-30
EP2345662A1 (en) 2011-07-20
US9758799B2 (en) 2017-09-12
US8440451B2 (en) 2013-05-14
US7943366B2 (en) 2011-05-17
PL2301949T3 (pl) 2015-10-30
BRPI0409954A (pt) 2006-04-25
US20060234364A1 (en) 2006-10-19
WO2004099381A3 (en) 2005-05-06
PL1626979T3 (pl) 2012-09-28
EP2338895B1 (en) 2013-08-14

Similar Documents

Publication Publication Date Title
DK2345662T3 (da) Genetisk modificerede gærarter og fermenteringsprocesser anvendende genetisk modificeret gær
US10899544B2 (en) Fermentation process using yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol
US8097448B2 (en) Genetically modified yeast of the species Issatchenkia orientalis and closely relates species, and fermentation processes using same
EP3033413B2 (en) Methods for the improvement of product yield and production in a microorganism through glycerol recycling
CN104126011A (zh) 由乙酸和甘油生产乙醇的工程化酵母菌株
CA2631036A1 (en) Lactic acid-producing yeast cells having nonfunctional l-or d-lactate: ferricytochrome c oxidoreductase gene
US9862975B2 (en) Compositions and methods for increased ethanol production from biomass