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DK2295558T3 - Xylanasevarianter - Google Patents

Xylanasevarianter Download PDF

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Publication number
DK2295558T3
DK2295558T3 DK10178666.3T DK10178666T DK2295558T3 DK 2295558 T3 DK2295558 T3 DK 2295558T3 DK 10178666 T DK10178666 T DK 10178666T DK 2295558 T3 DK2295558 T3 DK 2295558T3
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DK
Denmark
Prior art keywords
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Prior art date
Application number
DK10178666.3T
Other languages
English (en)
Inventor
Ole Sibbesen
Jens Frisbaek Soerensen
Original Assignee
Dupont Nutrition Biosci Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0005585A external-priority patent/GB0005585D0/en
Priority claimed from GB0015751A external-priority patent/GB0015751D0/en
Application filed by Dupont Nutrition Biosci Aps filed Critical Dupont Nutrition Biosci Aps
Application granted granted Critical
Publication of DK2295558T3 publication Critical patent/DK2295558T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Detergent Compositions (AREA)
  • Paper (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (11)

  1. XYLAN ASE V ARI ANTER
    1. Fremgangsmåde til ændring af sensitiviteten for et Familie-ll-xylanasepolypeptid over for en hæmmer, hvilken fremgangsmåde omfatter: (a) modificering af én eller flere af polypeptidets aminosyrerester; hvor modifikationerne er ved en hvilken som helst af positionerne 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 eller 175 af B. sntiif/fs-aminosyresekvensen ifølge SEQ. ID. NO. 1. eller ved én eller flere ækvivalente positioner i andre homologe xylanaser, hvor mindst én af aminosyremodifikationerne er mindst ved position 11 af B. sntiif/fs-aminosyresekvensen ifølge SEQ. ID. NO. 1 eller ved én eller flere ækvivalente positioner i andre homologe xylanaser; (b) måling af xylanasepolypeptidets sensitivitet over for en xylanasehæmmer og (c) måling af xylanasepolypeptidets effekt på en melopslæmnings viskositet; hvor peptidet eller fragmentet deraf har reduceret sensitivitet over for en xylanasehæmmer sammenlignet med moderxylanaseenzymet; hvor peptidet eller fragmentet deraf endvidere sænker en melopslæmnings viskositet mere end moderxylanaseenzymet.
  2. 2. Fremgangsmåde til ændring af sensitiviteten for et Familie-ll-xylanasepolypeptid over for en hæmmer, hvilken fremgangsmåde omfatter: (a) modificering af to eller flere polypeptidets aminosyrerester; hvor modifikationerne er af to eller flere af positionerne 11, 12, 13, 15, 17, 29, 31, 32, 34, 113, 114, 119, 120, 121, 122, 123, 124 og 175 af B. subtilis-aminosyresekvensen vist som SEQ. ID. NO. 1, eller ved én eller flere ækvivalente positioner i andre homologe xylanaser; hvor én af aminosyremodifikationerne er ved position 11; (b) måling af xylanasepolypeptidets sensitivitet over for en xylanasehæmmer og (c) måling af xylanasepolypeptidets effekt på en melopslæmnings viskositet; hvor polypeptidet eller fragmentet deraf har reduceret sensitivitet over for en xylanasehæmmer sammenlignet med moderxylanaseenzymet; endvidere hvor peptidet eller fragmentet deraf sænker en melopslæmnings viskositet mere end moderxylanaseenzymet.
  3. 3. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor aminosyremodifikationen sker ved målrettet mutagenese.
  4. 4. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor én af aminosyremodifikationerne er en hvilken som helst af: D11Y, DUN, D11F, D11K, DIIS, D11W, G12F, G13F, I15K, N17K, N17Y, N17D, N29K, N29Y, N29D, S31K, S31Y, S31D, N32K, G34D, G34F, G34T, Y113A, Y113D, Y113K, N114A, N114D, N114F, N114K, D119K, D119Y, D119N, G120K, G120D, G120F, G120Y, G120N, D121N, D121K, D121F, D121A, R122D, R122F, R122A, T123K, T123Y, T123D, T124K, T124Y, T124D, Q175E, Q175S og Q175L.
  5. 5. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor én af aminosyremodifikationerne er en hvilken som helst af: D11Y, DUN, D11F, D11K, DIIS eller D11W.
  6. 6. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor én af aminosyremodifikationeme erDllF.
  7. 7. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor to af aminosyremodifikationeme er D11F og R122D.
  8. 8. Anvendelse af en xylanasepolypeptidvariant tilvejebragt ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 7 i en fremgangsmåde til modificering af plantematerialer.
  9. 9. Anvendelse af en xylanasepolypeptidvariant tilvejebragt ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 7 i én eller flere af: bageri, dyrefoder, stivelsesproduktion, melseparation (vådformaling) og papir- og pulpproduktion.
  10. 10. Anvendelse af en xylanasepolypeptidvariant tilvejebragt ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 7 i én eller flere af: forarbejdning af korn, stivelsesproduktion, dyrefoder, i forarbejdning af træ, forbedring af processen til blegning af træpulp.
  11. 11. Anvendelse af en xylanasepolypeptidvariant tilvejebragt ved hjælp af fremgangsmåden ifølge et hvilket som helst af kravene 1 til 7 til at ændre viskositeten, der er afledt af tilstedeværelsen af hemicellulose eller arabinoxylan i en opløsning eller et system, der omfatter plantecellevægmateriale.
DK10178666.3T 2000-03-08 2001-03-08 Xylanasevarianter DK2295558T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0005585A GB0005585D0 (en) 2000-03-08 2000-03-08 Enzyme
GB0015751A GB0015751D0 (en) 2000-06-27 2000-06-27 Enzyme
EP01912068A EP1263941B1 (en) 2000-03-08 2001-03-08 Xylanase variants having altered sensitivity to xylanase inhibitors

Publications (1)

Publication Number Publication Date
DK2295558T3 true DK2295558T3 (da) 2018-01-22

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
DK10178666.3T DK2295558T3 (da) 2000-03-08 2001-03-08 Xylanasevarianter
DK01912068.2T DK1263941T3 (da) 2000-03-08 2001-03-08 Xylanasevarianter med ændret sensitivitet overfor xylanaseinhibitorer

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK01912068.2T DK1263941T3 (da) 2000-03-08 2001-03-08 Xylanasevarianter med ændret sensitivitet overfor xylanaseinhibitorer

Country Status (15)

Country Link
US (3) US7611881B2 (da)
EP (3) EP3339434A1 (da)
JP (1) JP4795604B2 (da)
AT (1) ATE491024T1 (da)
AU (1) AU785107C (da)
BR (1) BR0108750A (da)
CA (1) CA2399700C (da)
DE (1) DE60143606D1 (da)
DK (2) DK2295558T3 (da)
ES (1) ES2656440T3 (da)
MX (1) MXPA02008817A (da)
NZ (1) NZ520659A (da)
PL (1) PL212113B1 (da)
PT (1) PT1263941E (da)
WO (1) WO2001066711A1 (da)

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Also Published As

Publication number Publication date
US20090155413A1 (en) 2009-06-18
ATE491024T1 (de) 2010-12-15
JP2003525629A (ja) 2003-09-02
PL362558A1 (en) 2004-11-02
AU785107C (en) 2007-05-03
NZ520659A (en) 2005-06-24
AU785107B2 (en) 2006-09-14
PL212113B1 (pl) 2012-08-31
EP2295558A1 (en) 2011-03-16
WO2001066711A8 (en) 2001-11-01
JP4795604B2 (ja) 2011-10-19
US20030180895A1 (en) 2003-09-25
MXPA02008817A (es) 2003-02-12
EP3339434A1 (en) 2018-06-27
US20050271769A1 (en) 2005-12-08
US7611881B2 (en) 2009-11-03
AU4098401A (en) 2001-09-17
EP1263941B1 (en) 2010-12-08
PT1263941E (pt) 2011-01-20
EP2295558B1 (en) 2017-11-01
CA2399700C (en) 2013-10-01
DE60143606D1 (de) 2011-01-20
DK1263941T3 (da) 2011-03-14
EP1263941A1 (en) 2002-12-11
CA2399700A1 (en) 2001-09-13
BR0108750A (pt) 2002-12-24
ES2656440T3 (es) 2018-02-27
WO2001066711A1 (en) 2001-09-13
US7527957B2 (en) 2009-05-05

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