[go: up one dir, main page]

DK1370663T3 - Microbiological method for preparing L-carnitine - Google Patents

Microbiological method for preparing L-carnitine

Info

Publication number
DK1370663T3
DK1370663T3 DK02712878T DK02712878T DK1370663T3 DK 1370663 T3 DK1370663 T3 DK 1370663T3 DK 02712878 T DK02712878 T DK 02712878T DK 02712878 T DK02712878 T DK 02712878T DK 1370663 T3 DK1370663 T3 DK 1370663T3
Authority
DK
Denmark
Prior art keywords
carnitine
betaine
hydrolases
preparing
ester
Prior art date
Application number
DK02712878T
Other languages
Danish (da)
Inventor
Uwe Bornscheuer
Anna Musidlowska
Josef Werlen
Thomas Zimmermann
Benno Tscherry
Original Assignee
Lonza Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Ag filed Critical Lonza Ag
Application granted granted Critical
Publication of DK1370663T3 publication Critical patent/DK1370663T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/007—Carnitine; Butyrobetaine; Crotonobetaine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/41—Rhizobium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a method for producing L-carnitine on the basis of a betaine ester of the general formula (I), wherein R<1> and R<2> represent either hydrogen or together form a bond, and R<3> represents alkyl, aryl or arylalkyl, by means of hydrolases that convert said betaine ester to betaine. The betaine so obtained is then converted to L-carnitine by means of a microorganism capable of converting the betaine of formula (II) to L-carnitine. The invention further relates to hydrolases that convert 4-butyrobetaine methyl ester to 4-butyrobetaine and to microorganisms that contain such hydrolases.
DK02712878T 2001-01-31 2002-01-31 Microbiological method for preparing L-carnitine DK1370663T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01102214 2001-01-31
PCT/EP2002/001009 WO2002061094A2 (en) 2001-01-31 2002-01-31 Microbiological method for producing l-carnitine

Publications (1)

Publication Number Publication Date
DK1370663T3 true DK1370663T3 (en) 2007-07-02

Family

ID=8176358

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02712878T DK1370663T3 (en) 2001-01-31 2002-01-31 Microbiological method for preparing L-carnitine

Country Status (7)

Country Link
EP (1) EP1370663B1 (en)
CN (1) CN1233832C (en)
AT (1) ATE356214T1 (en)
DE (1) DE50209655D1 (en)
DK (1) DK1370663T3 (en)
ES (1) ES2282399T3 (en)
WO (1) WO2002061094A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2611901C (en) 2005-07-05 2012-12-11 Lonza Ag Spray-drying process for producing a dry carnitine powder or granulate
EP2325164A1 (en) * 2009-11-18 2011-05-25 Lonza Ltd. Methods for the production of l-carnitine
CN107879944B (en) * 2017-10-30 2020-05-22 杭州海尔希畜牧科技有限公司 Method for preparing butyl betaine
CN119120398B (en) * 2024-07-22 2025-09-09 华南师范大学 Enzyme mutant, enzyme composition or immobilized enzyme thereof, and application and method for preparing L-carnitine by using enzyme mutant and enzyme composition or immobilized enzyme

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI86889C (en) * 1984-03-29 1992-10-26 Lonza Ag Process for the preparation of L-carnitine in a microbiological manner
JPH0291049A (en) * 1988-09-21 1990-03-30 Lonza Ag Method for producing r-butyrobetaine
RU2099418C1 (en) * 1991-11-21 1997-12-20 Лонца Аг Dna fragment providing the betaine utilization, recombinant plasmid dna having dna fragment providing the betaine utilization, strain of bacterium rhizobium/agrobacterium assigned for stable storage of plasmid having dna fragment, a method of the bacterium strain preparing
CN1157480C (en) * 1993-10-08 2004-07-14 瑞士隆萨股份公司 Butyrobetaine/crotonbetaine-L-carnitine metabolic gene and its use in producing L-carnition by microbial method

Also Published As

Publication number Publication date
ES2282399T3 (en) 2007-10-16
ATE356214T1 (en) 2007-03-15
WO2002061094A3 (en) 2003-10-02
CN1233832C (en) 2005-12-28
CN1489629A (en) 2004-04-14
EP1370663B1 (en) 2007-03-07
WO2002061094A2 (en) 2002-08-08
EP1370663A2 (en) 2003-12-17
DE50209655D1 (en) 2007-04-19

Similar Documents

Publication Publication Date Title
DK1015416T3 (en) Process for the preparation of citalopram
PE20020951A1 (en) METHOD TO PRODUCE L-GLUTAMIC ACID
DK1249461T3 (en) Acid-blocked amine catalysts for the production of polyurethanes
TR199902941T2 (en) Plant transformation methods.
NZ515088A (en) Method for producing L-phosphinothricine by enzymatic transamination with aspartate
DK1255747T3 (en) Synthesis of 3,6-dialkyl-5,6-dihydro-4-hydroxy-pyran-2-one
MXPA04005766A (en) Method for the preparation of escitalopram.
ATE253550T1 (en) IN-SITU PRODUCTION OF A POLYASPARAGIC ACID ESTER MIXTURE
DK1127886T3 (en) Synthesis of 3,6-dialkyl-5,6-dihydro-4-hydroxy-2H-pyran-2-one
WO2002020525A3 (en) Protein tyrosine phosphatase inhibitor
DK1370663T3 (en) Microbiological method for preparing L-carnitine
ATE343385T1 (en) METHOD FOR PRODUCING DONEPEZIL
TW200407325A (en) Novel interleukin-1β converting enzyme inhibitors
BG107048A (en) Method for the preparation of citalopram
EP1439165A4 (en) INHIBITOR OF BIOSYNTHESIS OF ABSCISSIC ACID
MY122370A (en) Rhodococcus microorganisms and process for producing amide compounds.
ATE258605T1 (en) METHOD FOR PRODUCING (1R,4S)-2- AZABICYCLO 2.2.1 HEPT-5-EN-3-ON DERIVATIVES
DK1266967T3 (en) Process for the preparation of pravastatin and lovastatin
EA200000127A1 (en) METHOD OF OBTAINING AMINO DERIVATIVES OF ALKYLOXYFURANON OBTAINED BY THIS METHOD OF CONNECTION AND USE OF THESE COMPOUNDS
ATE272637T1 (en) METHOD FOR PRODUCING A SUBSTITUTED IMIDAZOPYRIDINE COMPOUND
NO20026198D0 (en) Cyclic ketones, their preparation and their use in the synthesis of amino acids
ATE279422T1 (en) METHOD FOR PRODUCING 1-HYDROXY-1, 1-DIPHOSPHONIC ACID COMPOUNDS
DK1082449T3 (en) Methods for stereoselective preparation of substituted cyclohexylcyanhydrins
DE60216616D1 (en) ENZYMATIC PROCESS FOR THE PRODUCTION OF ORGANO FLUOR COMPOUNDS
DK1504021T3 (en) Process for Dehydrogenation of Azaandrostan Compounds