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DK1356060T3 - Dobbeltarginintranslokation i Bacillus - Google Patents

Dobbeltarginintranslokation i Bacillus

Info

Publication number
DK1356060T3
DK1356060T3 DK01973140T DK01973140T DK1356060T3 DK 1356060 T3 DK1356060 T3 DK 1356060T3 DK 01973140 T DK01973140 T DK 01973140T DK 01973140 T DK01973140 T DK 01973140T DK 1356060 T3 DK1356060 T3 DK 1356060T3
Authority
DK
Denmark
Prior art keywords
bacillus
gram
host cell
protein
secretion
Prior art date
Application number
DK01973140T
Other languages
Danish (da)
English (en)
Inventor
Sierd Bron
Jan Douwe Harm Jongbloed
Joerg Paul Mueller
Dijl Jan Maarten Van
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of DK1356060T3 publication Critical patent/DK1356060T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cereal-Derived Products (AREA)
DK01973140T 2000-09-18 2001-09-17 Dobbeltarginintranslokation i Bacillus DK1356060T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23361000P 2000-09-18 2000-09-18

Publications (1)

Publication Number Publication Date
DK1356060T3 true DK1356060T3 (da) 2006-05-01

Family

ID=22877967

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01973140T DK1356060T3 (da) 2000-09-18 2001-09-17 Dobbeltarginintranslokation i Bacillus

Country Status (7)

Country Link
US (4) US7316924B2 (fr)
EP (1) EP1356060B1 (fr)
AT (1) ATE312932T1 (fr)
AU (1) AU2001292749A1 (fr)
DE (1) DE60115958T2 (fr)
DK (1) DK1356060T3 (fr)
WO (1) WO2002022667A2 (fr)

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US7655249B2 (en) * 1998-09-30 2010-02-02 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7901693B2 (en) * 1998-09-30 2011-03-08 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7329410B1 (en) 1998-09-30 2008-02-12 Corixa Corporation Compositions and method for WT1 specific immunotherapy
US7553494B2 (en) 2001-08-24 2009-06-30 Corixa Corporation WT1 fusion proteins
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
WO2003040335A2 (fr) * 2001-11-05 2003-05-15 Research Development Foundation Genie de peptides leader pour la secretion de proteines recombinees dans des bacteries
WO2003083056A2 (fr) * 2002-03-23 2003-10-09 Research Development Foundation Secretion de proteines a plusieurs liaisons disulfure chez des bacteries et leur utilisation
DE10256669B4 (de) 2002-12-04 2006-03-09 Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle Gemisch mindestens zweier Fusionsproteine sowie ihre Herstellung und Verwendung
DK1748077T3 (da) 2004-04-20 2013-04-08 Ajinomoto Kk Fremgangsmåde til fremstilling af protein
US20070026012A1 (en) * 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
EP1917347B1 (fr) * 2005-08-22 2015-10-07 Cornell Research Foundation, Inc. Compositions et methodes permettant d'analyser des interactions de proteines
KR100742218B1 (ko) 2005-10-21 2007-07-24 학교법인 포항공과대학교 트윈 아르기닌 트랜스로케이션 분비경로를 가지는오가노포스포러스 하이드롤라제 분비서열을 이용한대장균에서의 목적 외래 단백질 분비
WO2008051491A2 (fr) 2006-10-20 2008-05-02 Danisco Us, Inc. Genencor Division Polyol oxydases
CN101541968B (zh) 2006-11-30 2013-11-13 诺维信公司 重组宿主细胞中的脱氧核糖核酸酶表达
WO2008089132A2 (fr) * 2007-01-12 2008-07-24 Cornell Research Foundation, Inc. Sélection génétique pour le repliement des protéines et la solubilité dans le périplasme bactérien
AU2008267059A1 (en) * 2007-06-18 2008-12-24 Danisco Us, Inc., Genencor Division TAT signal peptides for producing proteins in prokaryotes
DE102008025926A1 (de) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Sekretionsoptimierter Mikroorganismus
DE102008025791A1 (de) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Sekretionsoptimierter Mikroorganismus
PT3044320T (pt) * 2013-09-11 2020-04-13 Impossible Foods Inc Secreção de polipéptidos contendo heme
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
EP4039695B1 (fr) 2015-05-11 2025-07-30 Impossible Foods Inc. Constructions d'expression et procédés de modification génétique de la levure méthylotrophique
KR101791526B1 (ko) * 2016-02-18 2017-11-01 (주)케어젠 발모 촉진 활성을 나타내는 펩타이드 및 이의 용도
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
GB201713732D0 (en) * 2017-08-25 2017-10-11 Alta Innovations Ltd Tat expression system
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
BR112021021154A2 (pt) 2019-04-25 2021-12-14 Impossible Foods Inc Cepas e métodos para produção de proteínas que contêm heme
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Family Cites Families (12)

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Publication number Priority date Publication date Assignee Title
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
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US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
IE910663A1 (en) * 1990-02-28 1991-08-28 Gist Brocades Nv Improved processing of proteins
US5641671A (en) 1990-07-06 1997-06-24 Unilever Patent Holdings B.V. Production of active Pseudomonas glumae lipase in homologous or heterologous hosts
EP1068339B1 (fr) 1998-04-01 2008-07-23 The Governors of the University of Alberta Compositions et methodes de secretion proteique
DK1354056T3 (en) 2000-10-10 2015-02-23 Danisco Us Inc IMPROVED secretion of a polypeptide by a microorganism

Also Published As

Publication number Publication date
AU2001292749A1 (en) 2002-03-26
US20090093025A1 (en) 2009-04-09
EP1356060B1 (fr) 2005-12-14
ATE312932T1 (de) 2005-12-15
US7884192B2 (en) 2011-02-08
WO2002022667A2 (fr) 2002-03-21
EP1356060A2 (fr) 2003-10-29
US7884191B2 (en) 2011-02-08
US20020110860A1 (en) 2002-08-15
US20080166757A1 (en) 2008-07-10
DE60115958T2 (de) 2006-08-03
WO2002022667A9 (fr) 2003-03-20
US7316924B2 (en) 2008-01-08
DE60115958D1 (en) 2006-01-19
US7897742B2 (en) 2011-03-01
US20080248525A1 (en) 2008-10-09
WO2002022667A3 (fr) 2003-09-04

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