DK1219713T3 - Fremgangsmåde til fremstilling af transglutaminase - Google Patents
Fremgangsmåde til fremstilling af transglutaminaseInfo
- Publication number
- DK1219713T3 DK1219713T3 DK00962986.6T DK00962986T DK1219713T3 DK 1219713 T3 DK1219713 T3 DK 1219713T3 DK 00962986 T DK00962986 T DK 00962986T DK 1219713 T3 DK1219713 T3 DK 1219713T3
- Authority
- DK
- Denmark
- Prior art keywords
- transglutaminase
- coryneform bacterium
- foreign protein
- pro
- secretory production
- Prior art date
Links
- 108060008539 Transglutaminase Proteins 0.000 title abstract 6
- 102000003601 transglutaminase Human genes 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 7
- 102000004169 proteins and genes Human genes 0.000 abstract 6
- 241000186254 coryneform bacterium Species 0.000 abstract 5
- 230000003248 secreting effect Effects 0.000 abstract 3
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/104—Aminoacyltransferases (2.3.2)
- C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/02—Aminoacyltransferases (2.3.2)
- C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP28009899 | 1999-09-30 | ||
| JP2000194043 | 2000-06-28 | ||
| PCT/JP2000/006780 WO2001023591A1 (en) | 1999-09-30 | 2000-09-29 | Process for producing transglutaminase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1219713T3 true DK1219713T3 (da) | 2011-12-12 |
Family
ID=26553616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK00962986.6T DK1219713T3 (da) | 1999-09-30 | 2000-09-29 | Fremgangsmåde til fremstilling af transglutaminase |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7723067B2 (da) |
| EP (1) | EP1219713B1 (da) |
| JP (1) | JP4320769B2 (da) |
| KR (1) | KR100565030B1 (da) |
| CN (1) | CN100497632C (da) |
| AT (1) | ATE532870T1 (da) |
| AU (1) | AU7449400A (da) |
| BR (1) | BR0014059B1 (da) |
| CA (1) | CA2391961C (da) |
| DK (1) | DK1219713T3 (da) |
| ES (1) | ES2372806T3 (da) |
| WO (1) | WO2001023591A1 (da) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2419453C (en) | 2000-08-17 | 2010-03-09 | Ajinomoto Co., Inc. | Mutant transglutaminase from streptoverticillium having modified substrate specificity |
| BR0208136B1 (pt) * | 2001-03-30 | 2014-10-21 | Ajinomoto Kk | Método para produzir uma proteína heteróloga |
| US7101695B2 (en) | 2002-03-01 | 2006-09-05 | Szu-Yi Chou | Method of producing transglutaminase having broad substrate activity |
| US7485438B2 (en) | 2002-03-01 | 2009-02-03 | Szu-Yi Chou | Method of producing polyvalent antigens |
| JP4323147B2 (ja) * | 2002-09-10 | 2009-09-02 | 天野エンザイム株式会社 | トランスグルタミナーゼ生産菌 |
| EP1548116A4 (en) * | 2002-09-30 | 2006-04-05 | Ajinomoto Kk | PROCESS FOR PRODUCING A PROTEIN MARKED BY A STABLE ISOTOPE |
| CA2518049C (en) | 2003-03-07 | 2013-05-07 | Ajinomoto Co., Inc. | Method of producing microbial transglutaminase |
| EP1748077B1 (en) * | 2004-04-20 | 2013-02-27 | Ajinomoto Co., Inc. | Method of producing protein |
| WO2007020291A1 (en) * | 2005-08-18 | 2007-02-22 | Novo Nordisk Health Care Ag | Improving transglutaminase substrate specificity |
| JP2010500886A (ja) * | 2006-08-18 | 2010-01-14 | ノボ ノルディスク ヘルス ケア アーゲー | 改善された特異性を有するトランスグルタミナーゼ変異体 |
| AU2008215422B2 (en) | 2007-02-15 | 2011-07-14 | Ajinomoto Co., Inc. | Transglutaminase having disulfide bond introduced therein |
| JP2010518842A (ja) * | 2007-02-22 | 2010-06-03 | ノボ ノルディスク ヘルス ケア アーゲー | 改善された特異性を有するトランスグルタミナーゼ変異体 |
| ES2480540T3 (es) | 2007-03-29 | 2014-07-28 | Ajinomoto Co., Inc. | Preparación enzimática para la adherencia y procedimiento de producción de un producto alimenticio moldeado por adherencia |
| KR20100085083A (ko) | 2007-11-19 | 2010-07-28 | 아지노모토 가부시키가이샤 | 섬유 가공물 및 이의 제조법 |
| ES2616020T3 (es) | 2008-02-13 | 2017-06-09 | Amano Enzyme Inc. | Transglutaminasa estabilizada y procedimiento para la producción de la misma |
| TWI444146B (zh) | 2008-09-25 | 2014-07-11 | Ajinomoto Kk | 黏著成型食品用酵素製劑及黏著成型食品之製造方法 |
| EP2404999B1 (en) | 2009-03-06 | 2015-04-29 | Ajinomoto Co., Inc. | Thermotolerant transglutaminase originating in actinomyces |
| US20100316764A1 (en) * | 2009-06-10 | 2010-12-16 | Engrain, LLC | Flour supplement compositions and methods for preparing wheat flour |
| CN103917656B (zh) * | 2011-10-25 | 2016-09-28 | 味之素株式会社 | 蛋白质的分泌产生方法 |
| EP2774982B1 (en) | 2011-11-02 | 2017-06-21 | Ajinomoto Co., Inc. | Method for secreting and producing proteins |
| IN2014CN04039A (da) | 2011-11-02 | 2015-10-23 | Ajinomoto Kk | |
| CN102586167B (zh) * | 2012-03-01 | 2013-07-24 | 华南理工大学 | 一株重组的枯草芽孢杆菌及由其生产转谷氨酰胺酶的方法 |
| CN103060283B (zh) * | 2012-12-28 | 2014-05-28 | 天津科技大学 | 一种抑制枯草芽孢杆菌谷氨酰胺转胺酶活性的多肽及其筛选方法和应用 |
| WO2014126260A1 (ja) * | 2013-02-18 | 2014-08-21 | 味の素株式会社 | タンパク質の沈殿による製造方法 |
| CN103146661A (zh) * | 2013-03-07 | 2013-06-12 | 哈尔滨工业大学 | MgCl2胁迫提高茂原链霉菌谷氨酰胺转氨酶产量的方法 |
| RU2013119826A (ru) | 2013-04-29 | 2014-11-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Коринеформная бактерия и способ получения гетерологичных гибридных белков |
| CN107532163A (zh) | 2015-04-24 | 2018-01-02 | 味之素株式会社 | 蛋白质的分泌生产方法 |
| US11242545B2 (en) | 2016-09-01 | 2022-02-08 | Ningxia Eppen Biotech Co., Ltd | Corynebacterium for producing L-lysine by fermentation |
| KR102335301B1 (ko) | 2016-10-21 | 2021-12-06 | 아지노모토 가부시키가이샤 | 단백질의 분비 생산법 |
| KR102337932B1 (ko) | 2016-10-21 | 2021-12-13 | 아지노모토 가부시키가이샤 | 단백질의 분비 생산법 |
| JP7613833B2 (ja) | 2017-03-28 | 2025-01-15 | 味の素株式会社 | Ｒｎａの製造方法 |
| WO2019078095A1 (en) | 2017-10-16 | 2019-04-25 | Ajinomoto Co., Inc. | PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY |
| CN107574159B (zh) * | 2017-10-26 | 2020-05-08 | 江南大学 | 一种以活性形式表达的谷氨酰胺转氨酶的突变体 |
| WO2019163827A1 (ja) | 2018-02-20 | 2019-08-29 | 味の素株式会社 | Ｒｎａサイレンシングを誘導する方法 |
| JP7439749B2 (ja) | 2018-04-20 | 2024-02-28 | 味の素株式会社 | タンパク質の分泌生産法 |
| WO2021183680A1 (en) * | 2020-03-13 | 2021-09-16 | Curie Co. Inc. | Transglutaminase variants |
| CN113528479B (zh) * | 2020-04-13 | 2023-01-24 | 中国科学院微生物研究所 | 一种转谷氨酰胺酶的高效制备方法及其专用工程菌 |
| CN115836127A (zh) | 2020-06-11 | 2023-03-21 | 味之素株式会社 | 蛋白的制造方法 |
| CN111944778B (zh) * | 2020-08-14 | 2022-06-21 | 安徽医学高等专科学校 | 谷氨酰胺转胺酶突变体及其编码基因和应用 |
| AU2022306337A1 (en) | 2021-07-07 | 2024-02-01 | Ajinomoto Co., Inc. | Method for secretory production of unnatural-amino-acid-containing protein |
| EP4509613A1 (en) | 2022-04-15 | 2025-02-19 | Ajinomoto Co., Inc. | Method for producing cultured meat |
| US20250304927A1 (en) * | 2022-05-20 | 2025-10-02 | Curie Co. Inc. | Pro-peptide variants for modulating activity of transglutaminases |
| AU2024221604A1 (en) | 2023-02-16 | 2025-09-11 | Ajinomoto Co., Inc. | Method for secretory production of proteins |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES8800352A1 (es) * | 1985-04-05 | 1987-10-16 | Sankyo Co | Un procedimiento para producir un compuesto capaz de funcionar como una elastasa pancreatica humana |
| RU2091490C1 (ru) | 1985-10-25 | 1997-09-27 | Зимодженетикс, Инк. | Способ получения гетерологичного полипептида в эукариотических микроорганизмов |
| US4965194A (en) * | 1986-08-21 | 1990-10-23 | Toyo Boseki Kabushiki Kaisha | Pyruvate oxidase and an analytical method using the same |
| WO1988009821A1 (en) * | 1987-06-12 | 1988-12-15 | Massachusetts Institute Of Technology | Coryneform expression and secretion system |
| JPH05244947A (ja) * | 1992-03-04 | 1993-09-24 | Mercian Corp | 新規ポストプロリンエンドペプチダーゼおよびその製造方法 |
| JPH0736754B2 (ja) | 1993-07-16 | 1995-04-26 | 三共株式会社 | ヒト・膵臓エラスターゼｉｉｉｂ |
| JPH09316095A (ja) * | 1996-05-27 | 1997-12-09 | Mitsubishi Chem Corp | 新規シグナルペプチド |
| JP3711658B2 (ja) * | 1996-10-07 | 2005-11-02 | 味の素株式会社 | コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質 |
| JPH10244947A (ja) | 1997-03-06 | 1998-09-14 | Toyoda Gosei Co Ltd | ステアリングホイール芯金 |
| JP3305609B2 (ja) | 1997-03-06 | 2002-07-24 | ダイハツ工業株式会社 | 車両用スペアタイヤの支持構造 |
-
2000
- 2000-09-29 KR KR1020027004159A patent/KR100565030B1/ko not_active Expired - Lifetime
- 2000-09-29 DK DK00962986.6T patent/DK1219713T3/da active
- 2000-09-29 EP EP00962986A patent/EP1219713B1/en not_active Expired - Lifetime
- 2000-09-29 CA CA002391961A patent/CA2391961C/en not_active Expired - Lifetime
- 2000-09-29 AU AU74494/00A patent/AU7449400A/en not_active Abandoned
- 2000-09-29 BR BRPI0014059-7A patent/BR0014059B1/pt active IP Right Grant
- 2000-09-29 ES ES00962986T patent/ES2372806T3/es not_active Expired - Lifetime
- 2000-09-29 CN CNB008137331A patent/CN100497632C/zh not_active Expired - Lifetime
- 2000-09-29 WO PCT/JP2000/006780 patent/WO2001023591A1/ja not_active Ceased
- 2000-09-29 JP JP2001526973A patent/JP4320769B2/ja not_active Expired - Lifetime
- 2000-09-29 AT AT00962986T patent/ATE532870T1/de active
-
2002
- 2002-04-01 US US10/112,488 patent/US7723067B2/en not_active Expired - Fee Related
-
2010
- 2010-02-26 US US12/714,151 patent/US7972829B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| BR0014059A (pt) | 2002-05-14 |
| BR0014059B1 (pt) | 2013-02-05 |
| KR100565030B1 (ko) | 2006-03-30 |
| EP1219713B1 (en) | 2011-11-09 |
| ATE532870T1 (de) | 2011-11-15 |
| KR20020038787A (ko) | 2002-05-23 |
| CA2391961C (en) | 2008-12-02 |
| EP1219713A1 (en) | 2002-07-03 |
| CA2391961A1 (en) | 2001-04-05 |
| JP4320769B2 (ja) | 2009-08-26 |
| EP1219713A4 (en) | 2005-02-02 |
| US20100297729A1 (en) | 2010-11-25 |
| ES2372806T3 (es) | 2012-01-26 |
| CN100497632C (zh) | 2009-06-10 |
| AU7449400A (en) | 2001-04-30 |
| WO2001023591A1 (en) | 2001-04-05 |
| CN1377413A (zh) | 2002-10-30 |
| US7972829B2 (en) | 2011-07-05 |
| US20030082746A1 (en) | 2003-05-01 |
| US7723067B2 (en) | 2010-05-25 |
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