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DK1283875T3 - Forbedrede nukleinsyre-modificerende enzymer - Google Patents

Forbedrede nukleinsyre-modificerende enzymer

Info

Publication number
DK1283875T3
DK1283875T3 DK01941707T DK01941707T DK1283875T3 DK 1283875 T3 DK1283875 T3 DK 1283875T3 DK 01941707 T DK01941707 T DK 01941707T DK 01941707 T DK01941707 T DK 01941707T DK 1283875 T3 DK1283875 T3 DK 1283875T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
modifying enzymes
enhanced nucleic
enzyme
acid
Prior art date
Application number
DK01941707T
Other languages
English (en)
Inventor
Yan Wang
Original Assignee
Bio Rad Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories filed Critical Bio Rad Laboratories
Application granted granted Critical
Publication of DK1283875T3 publication Critical patent/DK1283875T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1276RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07049RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07007DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK01941707T 2000-05-26 2001-05-29 Forbedrede nukleinsyre-modificerende enzymer DK1283875T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20756700P 2000-05-26 2000-05-26
US09/640,958 US6627424B1 (en) 2000-05-26 2000-08-16 Nucleic acid modifying enzymes
PCT/US2001/017492 WO2001092501A1 (en) 2000-05-26 2001-05-29 Improved nucleic acid modifying enzymes

Publications (1)

Publication Number Publication Date
DK1283875T3 true DK1283875T3 (da) 2007-11-26

Family

ID=26902363

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01941707T DK1283875T3 (da) 2000-05-26 2001-05-29 Forbedrede nukleinsyre-modificerende enzymer

Country Status (10)

Country Link
US (10) US6627424B1 (da)
EP (1) EP1283875B1 (da)
JP (2) JP4405725B2 (da)
AT (1) ATE368104T1 (da)
AU (2) AU7503901A (da)
CA (1) CA2409417C (da)
DE (1) DE60129549T2 (da)
DK (1) DK1283875T3 (da)
ES (1) ES2291322T3 (da)
WO (1) WO2001092501A1 (da)

Families Citing this family (126)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
US8268605B2 (en) 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
US20040009486A1 (en) * 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
EP1925669B1 (en) * 2000-02-17 2010-12-08 Qiagen GmbH Thermostable chimeric nucleic acid polymerases and uses thereof
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
DE60228618D1 (de) * 2001-11-28 2008-10-09 Bio Rad Laboratories Verfahren zur verwendung verbesserter polymerasen
DE60235491D1 (de) * 2001-11-28 2010-04-08 Bio Rad Laboratories Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
US20060234227A1 (en) * 2002-05-14 2006-10-19 Alexei Slesarev Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes
US7560260B2 (en) 2002-07-25 2009-07-14 Bio-Rad Laboratories, Inc. Compositions with polymerase activity
EP2264155A1 (en) 2002-07-25 2010-12-22 Bio-Rad Laboratories, Inc. Hybrid polymerase methods and compositions
US7666645B2 (en) * 2002-10-23 2010-02-23 Bio-Rad Laboratories, Inc. Sso7-polymerase conjugate proteins
WO2004038007A2 (en) * 2002-10-25 2004-05-06 Stratagene Dna polymerases with reduced base analog detection activity
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
DE602004025801D1 (de) * 2003-03-25 2010-04-15 Stratagene California Dna-polymerasefusionen und deren verwendungen
US7417133B2 (en) * 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
CA2891010C (en) * 2004-07-23 2022-09-20 Acceleron Pharma Inc. Actrii receptor polypeptides, methods and compositions
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
EP1836319B1 (en) * 2005-01-06 2012-09-19 Life Technologies Corporation Polypeptides having nucleic acid binding activity and methods for nucleic acid amplification
WO2006074288A2 (en) * 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity
WO2007050125A2 (en) * 2005-05-27 2007-05-03 William Marsh Rice University High processivity polymerases
WO2007008728A2 (en) * 2005-07-07 2007-01-18 Quanta Biosciences, Inc. Compositions and methods for increasing amplification efficiency
US9040276B2 (en) 2005-07-15 2015-05-26 Agilent Technologies, Inc. DNA binding protein-polymerase chimeras
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
EP1973559B1 (en) 2005-11-23 2013-01-09 Acceleron Pharma Inc. Activin-actriia antagonists and uses for promoting bone growth
US8128933B2 (en) 2005-11-23 2012-03-06 Acceleron Pharma, Inc. Method of promoting bone growth by an anti-activin B antibody
JP5106416B2 (ja) * 2006-01-06 2012-12-26 アジレント・テクノロジーズ・インク 濃縮(packed)DNAポリメラーゼを含む核酸複製のための反応緩衝液組成物
EP2057289B1 (en) 2006-08-09 2016-08-10 Univ Singapore PROCESS FOR MOLECULAR BIOLOGICAL APPLICATIONS
US8895016B2 (en) * 2006-12-18 2014-11-25 Acceleron Pharma, Inc. Antagonists of activin-actriia and uses for increasing red blood cell levels
ES2415666T3 (es) 2007-02-01 2013-07-26 Acceleron Pharma, Inc. Composiciones farmacéuticas que comprenden antagonistas de Activina-ActRIIa para uso en la prevención o el tratamiento de metástasis de cáncer de mama o pérdida ósea relacionada con el cáncer de mama
TWI782836B (zh) 2007-02-02 2022-11-01 美商艾瑟勒朗法瑪公司 衍生自ActRIIB的變體與其用途
CN101687016B (zh) 2007-02-09 2014-12-31 阿塞勒隆制药公司 活化素-actriia拮抗剂和促进癌症患者骨骼生长的用途
US7960343B2 (en) 2007-09-18 2011-06-14 Acceleron Pharma Inc. Activin-ActRIIa antagonists and uses for decreasing or inhibiting FSH secretion
WO2009070604A1 (en) * 2007-11-27 2009-06-04 Bio-Rad Laboratories, Inc. Reduced inhibition of one-step rt-pcr
EP2240576B1 (en) * 2008-01-11 2012-10-03 Genesys Ltd Cren7 chimeric protein
GB0803628D0 (en) 2008-02-28 2008-04-02 Genesys Ltd Enzyme
GB0804722D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
GB0804721D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
ES2949049T3 (es) 2008-08-14 2023-09-25 Acceleron Pharma Inc Trampas de GDF
US8216997B2 (en) 2008-08-14 2012-07-10 Acceleron Pharma, Inc. Methods for increasing red blood cell levels and treating anemia using a combination of GDF traps and erythropoietin receptor activators
JP5258512B2 (ja) * 2008-10-30 2013-08-07 株式会社日立製作所 エキソヌクレアーゼ活性増強dnaポリメラーゼ変異体
WO2010062776A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
CN102245761A (zh) 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶
CN102272319B (zh) * 2009-01-08 2014-08-27 伯乐实验室公司 用于提高核酸扩增反应效率的方法和组合物
US10113156B2 (en) * 2009-03-04 2018-10-30 Board Of Regents, The University Of Texas System Stabilized reverse transcriptase fusion proteins
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
WO2010111691A2 (en) 2009-03-27 2010-09-30 Life Technologies Corp Conjugates of biomolecules to nanoparticles
US8535925B2 (en) 2009-04-10 2013-09-17 Bio-Rad Laboratories, Inc. Modified DNase compositions and methods of use thereof
CN104840944A (zh) 2009-06-08 2015-08-19 阿塞勒隆制药公司 用于增加产热脂肪细胞的方法
CN107267520A (zh) 2009-06-12 2017-10-20 阿塞勒隆制药公司 截短的actriib‑fc融合蛋白
US8889394B2 (en) * 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
CN102781518A (zh) * 2009-09-09 2012-11-14 阿塞勒隆制药公司 Actriib拮抗剂及其给药和用途
JP2013505016A (ja) * 2009-09-16 2013-02-14 マッシー ユニヴァーシティー 融合ポリペプチドおよびその使用
JP6267425B2 (ja) 2009-11-17 2018-01-24 アクセルロン ファーマ, インコーポレイテッド 筋ジストロフィー治療のためのユートロフィン誘導に関するactriibタンパク質およびその改変体およびその使用
IN2012DN05145A (da) * 2009-11-19 2015-10-23 Solis Biodyne
EP2808401B1 (en) 2010-02-26 2016-12-14 Life Technologies Corporation Method for sequencing using a modified DNA polymerase
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
WO2012034007A2 (en) 2010-09-10 2012-03-15 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
WO2012046140A1 (en) 2010-10-05 2012-04-12 Finnzymes Oy Enzyme mixture
KR20130132824A (ko) 2010-11-08 2013-12-05 악셀레론 파마 인코포레이티드 Actriia 결합제 및 이의 용도
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
WO2012109315A1 (en) 2011-02-08 2012-08-16 Life Technologies Corporation Linking methods, compositions, systems, kits and apparatuses
US20120208193A1 (en) 2011-02-15 2012-08-16 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
WO2012125123A1 (en) 2011-03-11 2012-09-20 National University Of Singapore Pericyte progenitors from peripheral blood
JP6072766B2 (ja) 2011-04-08 2017-02-01 バイオ−ラッド ラボラトリーズ インコーポレーティッド 非特異的活性が低下したpcr反応混合物
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
EP2707481B1 (en) 2011-05-11 2016-07-13 New England Biolabs, Inc. Dna polymerase variants with reduced exonuclease activity and uses thereof
US8921044B2 (en) 2011-05-11 2014-12-30 New England Biolabs, Inc. DNA polymerase variants with reduced exonuclease activity and uses thereof
US8470573B2 (en) * 2011-06-21 2013-06-25 Bio-Rad Laboratories, Inc. Hybrid polymerases having the ability to produce long amplicons
US8728987B2 (en) 2011-08-03 2014-05-20 Bio-Rad Laboratories, Inc. Filtering small nucleic acids using permeabilized cells
EP3964224A1 (en) 2012-11-02 2022-03-09 Celgene Corporation Activin-actrii antagonists and uses for use in treating renal disease
EP2986719B1 (en) 2013-12-06 2020-07-29 Bio-Rad Laboratories, Inc. Fusion polymerases
CN107135646B (zh) 2014-06-13 2022-03-15 阿塞勒隆制药公司 用于治疗溃疡的方法和组合物
WO2016014493A1 (en) 2014-07-22 2016-01-28 Bio-Rad Laboratories, Inc. Buffers for use with polymerases
CN106661632B (zh) 2014-08-19 2021-11-05 生物辐射实验室股份有限公司 Rna扩增方法
MA41052A (fr) 2014-10-09 2017-08-15 Celgene Corp Traitement d'une maladie cardiovasculaire à l'aide de pièges de ligands d'actrii
WO2016077795A1 (en) 2014-11-14 2016-05-19 Illumina, Inc. Polymerases
EP3224359B1 (en) 2014-11-25 2023-10-25 Bio-Rad Laboratories, Inc. Arginine improves polymerase storage stability
ES2946160T3 (es) 2014-12-03 2023-07-13 Celgene Corp Antagonistas de activina-ActRII y usos para tratar síndrome mielodisplásico
JP6736565B2 (ja) 2015-02-02 2020-08-05 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft ポリメラーゼバリアント
EP3259373B1 (en) 2015-02-17 2022-07-27 Bio-Rad Laboratories, Inc. Small nucleic acid quantification using split cycle amplification
WO2017050723A1 (en) 2015-09-22 2017-03-30 Genia Technologies, Inc. Pol7 polymerase variants
CN108350087A (zh) * 2015-11-27 2018-07-31 国立大学法人九州大学 Dna聚合酶变体
WO2017123748A1 (en) 2016-01-13 2017-07-20 New England Biolabs, Inc. Thermostable variants of t7 rna polymerase
LT3402880T (lt) 2016-01-15 2024-03-12 Thermo Fisher Scientific Baltics Uab Termofiliniai dnr polimerazės mutantai
EP3402826A1 (en) 2016-01-15 2018-11-21 Thermo Fisher Scientific Baltics UAB Antibodies that bind thermophilic dna polymerases
EP3423575B1 (en) 2016-02-29 2021-06-16 Genia Technologies, Inc. Exonuclease deficient polymerases
WO2017148860A1 (en) 2016-02-29 2017-09-08 Genia Technologies, Inc. Polymerase-template complexes for nanopore sequencing
EP3929283A1 (en) 2016-02-29 2021-12-29 Genia Technologies, Inc. Polymerase variants
JP6720632B2 (ja) * 2016-03-29 2020-07-08 東洋紡株式会社 融合タンパク質
US10829747B2 (en) 2016-09-22 2020-11-10 Roche Sequencing Solutions, Inc. Polymerase variants
US11618891B2 (en) * 2017-06-26 2023-04-04 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
EP3728633A1 (en) 2017-12-22 2020-10-28 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
US11136565B2 (en) 2018-09-11 2021-10-05 Singular Genomics Systems, Inc. Modified archaeal family B polymerases
CN109266628B (zh) * 2018-10-09 2020-04-14 南京市胸科医院 一种融合的TaqDNA聚合酶及其应用
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN109679932B (zh) * 2018-12-05 2020-03-17 广州奇辉生物科技有限公司 一种dna聚合酶、重组载体及它们的制备方法和应用
CN112175091A (zh) * 2019-07-05 2021-01-05 赛纳生物科技(北京)有限公司 通过功能结构域嫁接提高聚合酶大片段活性的方法及应用
US12163164B2 (en) 2019-07-15 2024-12-10 Bio-Rad Laboratories, Inc. Hybrid reverse transcriptases
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
CA3165756A1 (en) * 2020-01-06 2021-07-15 Pairwise Plants Services, Inc. Recruitment of dna polymerase for templated editing
EP4103701A1 (en) 2020-02-13 2022-12-21 New England Biolabs, Inc. Variant family d dna polymerases
EP4103702A1 (en) 2020-02-13 2022-12-21 New England Biolabs, Inc. Variant family d dna polymerases
US20230086471A1 (en) * 2020-02-15 2023-03-23 Agency For Science, Technology And Research A method to amplify a nucleic acid
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
CA3174486A1 (en) 2020-03-04 2021-09-10 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
CN111518873B (zh) * 2020-05-11 2024-07-09 上海羿鸣生物科技有限公司 一种优化的扩增目标核酸的方法及应用
PL241698B1 (pl) * 2021-01-27 2022-11-21 Inst Biotechnologii I Medycyny Molekularnej Polimeraza Pwo-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy
US11486001B2 (en) 2021-02-08 2022-11-01 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits
EP4347804A1 (en) 2021-05-27 2024-04-10 New England Biolabs, Inc. Dnase i variants, compositions, methods, and kits
EP4095254A3 (en) 2021-05-27 2022-12-14 New England Biolabs, Inc. Fragmentation of dna
WO2022249133A1 (en) 2021-05-28 2022-12-01 Erebus Enzymes Inc. Ultra-processive, stutter-resistant polymerase system
CA3231677A1 (en) 2021-09-08 2023-03-16 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
EP4437090A1 (en) 2021-11-22 2024-10-02 F. Hoffmann-La Roche AG Thermostable polymerase variants
WO2023146243A1 (ko) 2022-01-26 2023-08-03 주식회사 씨젠 샘플 내 타겟 핵산을 검출하는 방법
WO2023219343A1 (ko) 2022-05-13 2023-11-16 주식회사 씨젠 샘플 핸들링 시스템을 이용하여 샘플 내 핵산 증폭 반응을 위한 반응 혼합물을 준비하는 방법
US20240263158A1 (en) 2023-02-01 2024-08-08 New England Biolabs, Inc. Duplex-specific dnases
WO2024244010A1 (zh) * 2023-06-02 2024-12-05 武汉华大智造科技有限公司 用于测序的dna聚合酶突变体
WO2025137293A2 (en) 2023-12-22 2025-06-26 Roche Sequencing Solutions, Inc. Enhancement of nucleic acid polymerization by aromatic compounds
WO2025227034A1 (en) 2024-04-26 2025-10-30 New England Biolabs, Inc. Salt active nuclease compositions and methods

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0503000A4 (en) * 1989-12-01 1993-04-14 The Board Of Trustees Of The Leland Stanford Junior University Promotion of high specificity molecular assembly
US5747911A (en) 1994-09-30 1998-05-05 Itt Automotive Electrical Systems, Inc. Brush holder
US5972603A (en) * 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
DE19810879A1 (de) * 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
DE19840771A1 (de) * 1998-08-06 2000-02-10 Lion Bioscience Ag Thermostabiler in vitro-Komplex mit Polymeraseaktivität
WO2000008164A2 (de) * 1998-08-06 2000-02-17 Lion Bioscience Ag Thermostabiler in vitro-komplex mit polymeraseaktivität
EP1925669B1 (en) 2000-02-17 2010-12-08 Qiagen GmbH Thermostable chimeric nucleic acid polymerases and uses thereof
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes

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US7670808B2 (en) 2010-03-02
AU2001275039B2 (en) 2006-07-13
US8895283B2 (en) 2014-11-25
US9453208B2 (en) 2016-09-27
US20040191825A1 (en) 2004-09-30
JP2003534796A (ja) 2003-11-25
ES2291322T3 (es) 2008-03-01
US8900846B2 (en) 2014-12-02
AU7503901A (en) 2001-12-11
US20150218536A1 (en) 2015-08-06
US8415129B2 (en) 2013-04-09
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JP4405725B2 (ja) 2010-01-27
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US7919296B2 (en) 2011-04-05
US6627424B1 (en) 2003-09-30
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ATE368104T1 (de) 2007-08-15

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