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DK0846164T3 - Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease - Google Patents

Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease

Info

Publication number
DK0846164T3
DK0846164T3 DK96926574T DK96926574T DK0846164T3 DK 0846164 T3 DK0846164 T3 DK 0846164T3 DK 96926574 T DK96926574 T DK 96926574T DK 96926574 T DK96926574 T DK 96926574T DK 0846164 T3 DK0846164 T3 DK 0846164T3
Authority
DK
Denmark
Prior art keywords
protease
hcv
polypeptides
proteolytic activity
matter
Prior art date
Application number
DK96926574T
Other languages
Danish (da)
English (en)
Inventor
Licia Tomei
Christian Steinkuehler
Antonello Pessi
Elisabetta Bianchi
Marina Taliani
Andrea Urbani
Francesco Raffaele De
Frank Narjes
Original Assignee
Angeletti P Ist Richerche Bio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=11403536&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0846164(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Angeletti P Ist Richerche Bio filed Critical Angeletti P Ist Richerche Bio
Application granted granted Critical
Publication of DK0846164T3 publication Critical patent/DK0846164T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
DK96926574T 1995-08-22 1996-08-20 Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease DK0846164T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IT95RM000573A IT1277914B1 (it) 1995-08-22 1995-08-22 Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e
EP96926574A EP0846164B1 (fr) 1995-08-22 1996-08-20 Procede pour tester l'activite proteolytique de polypeptides ayant une activite de protease hcv ns3 et peptides substrats a utiliser dans cette methode.
PCT/IT1996/000163 WO1997008304A2 (fr) 1995-08-22 1996-08-20 Methode de production, de purification et d'analyse de polypeptides par l'activite proteolytique de la vhc ns3 protease

Publications (1)

Publication Number Publication Date
DK0846164T3 true DK0846164T3 (da) 2005-06-27

Family

ID=11403536

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96926574T DK0846164T3 (da) 1995-08-22 1996-08-20 Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease

Country Status (14)

Country Link
US (1) US6197536B1 (fr)
EP (1) EP0846164B1 (fr)
JP (1) JP3310298B2 (fr)
CN (1) CN1193997A (fr)
AT (1) ATE290070T1 (fr)
AU (1) AU716379B2 (fr)
BR (1) BR9610039A (fr)
CA (1) CA2228265C (fr)
DE (1) DE69634406T2 (fr)
DK (1) DK0846164T3 (fr)
ES (1) ES2236743T3 (fr)
IT (1) IT1277914B1 (fr)
PT (1) PT846164E (fr)
WO (1) WO1997008304A2 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1285158B1 (it) * 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il
GB9623908D0 (en) * 1996-11-18 1997-01-08 Hoffmann La Roche Amino acid derivatives
GB9707659D0 (en) * 1997-04-16 1997-06-04 Peptide Therapeutics Ltd Hepatitis C NS3 Protease inhibitors
ES2143918B1 (es) * 1997-05-23 2001-01-01 Inst Cientifico Tecnol Navarra Peptidos inhibidores de la proteasa c y su utilizacion en la preparacion de composiciones utiles en las infecciones causadas por el virus de la hepatitis c.
WO1999028482A2 (fr) * 1997-11-28 1999-06-10 Schering Corporation Complexes recombinants monocatenaires de la protease ns3 et du peptide co-facteur ns4a du virus de l'hepatite c (vhc)
IT1299134B1 (it) * 1998-02-02 2000-02-29 Angeletti P Ist Richerche Bio Procedimento per la produzione di peptidi con proprieta' inibitrici della proteasi ns3 del virus hcv, peptidi cosi' ottenibili e peptidi
WO2000001718A2 (fr) * 1998-07-02 2000-01-13 University Of Florida Domaine catalytique ns4a et ns3 de l'hepatite c
US6323180B1 (en) * 1998-08-10 2001-11-27 Boehringer Ingelheim (Canada) Ltd Hepatitis C inhibitor tri-peptides
JP2002534084A (ja) 1999-01-08 2002-10-15 ブリストル−マイヤーズ スクイブ カンパニー Ｃ型肝炎ウイルスｎｓ３プロテアーゼの修飾形
HUP0200945A3 (en) * 1999-05-04 2004-07-28 Boehringer Ingelheim Ca Ltd Surrogate cell-based system and method for assaying the activity of hapatitis c virus ns3 protease
CA2383411A1 (fr) * 1999-08-30 2001-03-08 Paul L. Darke Inhibiteurs de replication du virus de l'hepatite c
GB0006537D0 (en) * 2000-03-17 2000-05-10 Angeletti P Ist Richerche Bio Assays and screening methods
ATE356875T1 (de) 2000-12-15 2007-04-15 Boehringer Ingelheim Ca Ltd Gereinigte aktive hcv ns2/3 protease
EP1558730B1 (fr) * 2002-10-29 2008-01-30 Boehringer Ingelheim International GmbH Protease ns3 du vhc resistante aux inhibiteurs
US7834145B2 (en) 2005-03-22 2010-11-16 Merck Sharp & Dohme Corp. HCV protease substrates
ES2386462T3 (es) * 2005-10-28 2012-08-21 Boehringer Ingelheim International Gmbh Ensayo de la actividad NS2/3 del virus de la hepatitis C
CN103012565B (zh) * 2013-01-15 2014-07-16 成都天台山制药有限公司 一种曲普瑞林及其固相合成制备方法
JP6564539B1 (ja) 2018-09-14 2019-08-21 長瀬産業株式会社 スルホン酸化合物によるペプチド精製方法
WO2025178073A1 (fr) * 2024-02-22 2025-08-28 天野エンザイム株式会社 Procédé pour confirmer qu'une enzyme protéolytique a été éliminée, et ses applications

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991015575A1 (fr) * 1990-04-04 1991-10-17 Chiron Corporation Protease du virus de l'hepatite c
US5885771A (en) * 1993-10-29 1999-03-23 Srl, Inc. Antigenic peptide compound and immunoassay
JPH08205893A (ja) * 1994-12-08 1996-08-13 Sumitomo Metal Ind Ltd Ｈｃｖプロテアーゼ活性測定法
US5767233A (en) * 1995-05-12 1998-06-16 Schering Corporation Soluble cleavable substrates of the hepatitis C virus protease

Also Published As

Publication number Publication date
BR9610039A (pt) 1999-07-06
JP3310298B2 (ja) 2002-08-05
AU716379B2 (en) 2000-02-24
ES2236743T3 (es) 2005-07-16
PT846164E (pt) 2005-07-29
IT1277914B1 (it) 1997-11-12
CA2228265C (fr) 2002-08-13
US6197536B1 (en) 2001-03-06
JPH10511556A (ja) 1998-11-10
CA2228265A1 (fr) 1997-03-06
CN1193997A (zh) 1998-09-23
ATE290070T1 (de) 2005-03-15
ITRM950573A0 (it) 1995-08-22
AU6668696A (en) 1997-03-19
DE69634406D1 (de) 2005-04-07
WO1997008304A2 (fr) 1997-03-06
WO1997008304A3 (fr) 1997-05-22
EP0846164B1 (fr) 2005-03-02
DE69634406T2 (de) 2006-04-13
EP0846164A2 (fr) 1998-06-10
ITRM950573A1 (it) 1997-02-22

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