DK0846164T3 - Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease - Google Patents
Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-proteaseInfo
- Publication number
- DK0846164T3 DK0846164T3 DK96926574T DK96926574T DK0846164T3 DK 0846164 T3 DK0846164 T3 DK 0846164T3 DK 96926574 T DK96926574 T DK 96926574T DK 96926574 T DK96926574 T DK 96926574T DK 0846164 T3 DK0846164 T3 DK 0846164T3
- Authority
- DK
- Denmark
- Prior art keywords
- protease
- hcv
- polypeptides
- proteolytic activity
- matter
- Prior art date
Links
- 101800001838 Serine protease/helicase NS3 Proteins 0.000 title abstract 6
- 229920001184 polypeptide Polymers 0.000 title abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 4
- 230000002797 proteolythic effect Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 238000003556 assay Methods 0.000 title 1
- 238000002360 preparation method Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 abstract 2
- 239000000137 peptide hydrolase inhibitor Substances 0.000 abstract 2
- 108010002156 Depsipeptides Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- OHRURASPPZQGQM-GCCNXGTGSA-N romidepsin Chemical compound O1C(=O)[C@H](C(C)C)NC(=O)C(=C/C)/NC(=O)[C@H]2CSSCC\C=C\[C@@H]1CC(=O)N[C@H](C(C)C)C(=O)N2 OHRURASPPZQGQM-GCCNXGTGSA-N 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 229940124597 therapeutic agent Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
- C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
- C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT95RM000573A IT1277914B1 (it) | 1995-08-22 | 1995-08-22 | Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e |
| EP96926574A EP0846164B1 (en) | 1995-08-22 | 1996-08-20 | Method for assaying in vitro the proteolytic activity of polypeptides having hcv ns3 protease activity and peptide substrates to be used in this method. |
| PCT/IT1996/000163 WO1997008304A2 (en) | 1995-08-22 | 1996-08-20 | Methodology to produce, purify and assay polypeptides with the proteolytic activity of the hcv ns3 protease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0846164T3 true DK0846164T3 (da) | 2005-06-27 |
Family
ID=11403536
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK96926574T DK0846164T3 (da) | 1995-08-22 | 1996-08-20 | Metodologi til fremstilling, oprensning og assay af polypeptider med den proteolytiske aktivitet af HCV NS3-protease |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US6197536B1 (da) |
| EP (1) | EP0846164B1 (da) |
| JP (1) | JP3310298B2 (da) |
| CN (1) | CN1193997A (da) |
| AT (1) | ATE290070T1 (da) |
| AU (1) | AU716379B2 (da) |
| BR (1) | BR9610039A (da) |
| CA (1) | CA2228265C (da) |
| DE (1) | DE69634406T2 (da) |
| DK (1) | DK0846164T3 (da) |
| ES (1) | ES2236743T3 (da) |
| IT (1) | IT1277914B1 (da) |
| PT (1) | PT846164E (da) |
| WO (1) | WO1997008304A2 (da) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1285158B1 (it) * | 1996-09-17 | 1998-06-03 | Angeletti P Ist Richerche Bio | Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il |
| GB9623908D0 (en) * | 1996-11-18 | 1997-01-08 | Hoffmann La Roche | Amino acid derivatives |
| GB9707659D0 (en) * | 1997-04-16 | 1997-06-04 | Peptide Therapeutics Ltd | Hepatitis C NS3 Protease inhibitors |
| ES2143918B1 (es) * | 1997-05-23 | 2001-01-01 | Inst Cientifico Tecnol Navarra | Peptidos inhibidores de la proteasa c y su utilizacion en la preparacion de composiciones utiles en las infecciones causadas por el virus de la hepatitis c. |
| WO1999028482A2 (en) * | 1997-11-28 | 1999-06-10 | Schering Corporation | Single-chain recombinant complexes of hepatitis c virus ns3 protease and ns4a cofactor peptide |
| IT1299134B1 (it) * | 1998-02-02 | 2000-02-29 | Angeletti P Ist Richerche Bio | Procedimento per la produzione di peptidi con proprieta' inibitrici della proteasi ns3 del virus hcv, peptidi cosi' ottenibili e peptidi |
| AU4855299A (en) * | 1998-07-02 | 2000-01-24 | University Of Florida | Ns4a-ns3 catalytic domain of hepatitis c |
| US6323180B1 (en) * | 1998-08-10 | 2001-11-27 | Boehringer Ingelheim (Canada) Ltd | Hepatitis C inhibitor tri-peptides |
| AU2494100A (en) | 1999-01-08 | 2000-07-24 | Bristol-Myers Squibb Company | Modified forms of hepatitis c virus ns3 protease |
| DE60038113T2 (de) * | 1999-05-04 | 2009-02-12 | Boehringer Ingelheim (Canada) Ltd., Laval | Zellen-internes system und verfahren zur untersuchung der aktivität der hepatitis c virus ns3 protease |
| CA2383411A1 (en) * | 1999-08-30 | 2001-03-08 | Paul L. Darke | Hepatitis c virus replication inhibitors |
| GB0006537D0 (en) * | 2000-03-17 | 2000-05-10 | Angeletti P Ist Richerche Bio | Assays and screening methods |
| ES2282320T3 (es) | 2000-12-15 | 2007-10-16 | Boehringer Ingelheim (Canada) Ltd. | Proteasa hcv ns2/3 activa, purificada. |
| CA2498653C (en) * | 2002-10-29 | 2010-12-14 | Boehringer Ingelheim International Gmbh | Inhibitor-resistant hcv ns3 protease |
| WO2006102087A2 (en) * | 2005-03-22 | 2006-09-28 | Merck & Co., Inc. | Hcv protease substrates |
| DK1945797T3 (da) * | 2005-10-28 | 2012-08-06 | Boehringer Ingelheim Int | Hepatitis C-virus NS2/3-aktivitetsanalyse |
| CN103012565B (zh) * | 2013-01-15 | 2014-07-16 | 成都天台山制药有限公司 | 一种曲普瑞林及其固相合成制备方法 |
| JP6564539B1 (ja) | 2018-09-14 | 2019-08-21 | 長瀬産業株式会社 | スルホン酸化合物によるペプチド精製方法 |
| WO2025178073A1 (ja) * | 2024-02-22 | 2025-08-28 | 天野エンザイム株式会社 | タンパク質分解酵素が除去されたことを確認する方法及びその応用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69133617D1 (de) * | 1990-04-04 | 2009-07-23 | Novartis Vaccines & Diagnostic | Protease von Hepatitis C Virus |
| AU8003894A (en) * | 1993-10-29 | 1995-05-22 | Srl Inc. | Antigen peptide compound and immunoassay method |
| JPH08205893A (ja) * | 1994-12-08 | 1996-08-13 | Sumitomo Metal Ind Ltd | Ｈｃｖプロテアーゼ活性測定法 |
| US5767233A (en) * | 1995-05-12 | 1998-06-16 | Schering Corporation | Soluble cleavable substrates of the hepatitis C virus protease |
-
1995
- 1995-08-22 IT IT95RM000573A patent/IT1277914B1/it active IP Right Grant
-
1996
- 1996-08-20 WO PCT/IT1996/000163 patent/WO1997008304A2/en not_active Ceased
- 1996-08-20 JP JP51008297A patent/JP3310298B2/ja not_active Expired - Fee Related
- 1996-08-20 AU AU66686/96A patent/AU716379B2/en not_active Ceased
- 1996-08-20 AT AT96926574T patent/ATE290070T1/de not_active IP Right Cessation
- 1996-08-20 DE DE69634406T patent/DE69634406T2/de not_active Revoked
- 1996-08-20 PT PT96926574T patent/PT846164E/pt unknown
- 1996-08-20 CA CA002228265A patent/CA2228265C/en not_active Expired - Fee Related
- 1996-08-20 ES ES96926574T patent/ES2236743T3/es not_active Expired - Lifetime
- 1996-08-20 DK DK96926574T patent/DK0846164T3/da active
- 1996-08-20 CN CN96196406A patent/CN1193997A/zh active Pending
- 1996-08-20 BR BR9610039A patent/BR9610039A/pt not_active Application Discontinuation
- 1996-08-20 EP EP96926574A patent/EP0846164B1/en not_active Revoked
-
1998
- 1998-02-23 US US09/011,961 patent/US6197536B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| PT846164E (pt) | 2005-07-29 |
| WO1997008304A3 (en) | 1997-05-22 |
| US6197536B1 (en) | 2001-03-06 |
| DE69634406T2 (de) | 2006-04-13 |
| ITRM950573A0 (it) | 1995-08-22 |
| EP0846164A2 (en) | 1998-06-10 |
| WO1997008304A2 (en) | 1997-03-06 |
| ATE290070T1 (de) | 2005-03-15 |
| CA2228265C (en) | 2002-08-13 |
| JP3310298B2 (ja) | 2002-08-05 |
| ITRM950573A1 (it) | 1997-02-22 |
| JPH10511556A (ja) | 1998-11-10 |
| AU716379B2 (en) | 2000-02-24 |
| AU6668696A (en) | 1997-03-19 |
| DE69634406D1 (de) | 2005-04-07 |
| ES2236743T3 (es) | 2005-07-16 |
| IT1277914B1 (it) | 1997-11-12 |
| BR9610039A (pt) | 1999-07-06 |
| EP0846164B1 (en) | 2005-03-02 |
| CA2228265A1 (en) | 1997-03-06 |
| CN1193997A (zh) | 1998-09-23 |
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