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DE69225074D1 - Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen - Google Patents

Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen

Info

Publication number
DE69225074D1
DE69225074D1 DE69225074T DE69225074T DE69225074D1 DE 69225074 D1 DE69225074 D1 DE 69225074D1 DE 69225074 T DE69225074 T DE 69225074T DE 69225074 T DE69225074 T DE 69225074T DE 69225074 D1 DE69225074 D1 DE 69225074D1
Authority
DE
Germany
Prior art keywords
pct
double stranded
stranded rna
applications
date jun
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69225074T
Other languages
English (en)
Other versions
DE69225074T2 (de
Inventor
Thierry Livache
Brigitte Fouque
Robert Teoule
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
CIS Bio International SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CIS Bio International SA filed Critical CIS Bio International SA
Publication of DE69225074D1 publication Critical patent/DE69225074D1/de
Application granted granted Critical
Publication of DE69225074T2 publication Critical patent/DE69225074T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69225074T 1991-12-18 1992-12-18 Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen Expired - Lifetime DE69225074T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9115710A FR2685346B1 (fr) 1991-12-18 1991-12-18 Procede de preparation d'arn double-brin, et ses applications.
PCT/FR1992/001209 WO1993012229A1 (fr) 1991-12-18 1992-12-18 Procede de preparation d'arn double-brin, et ses applications

Publications (2)

Publication Number Publication Date
DE69225074D1 true DE69225074D1 (de) 1998-05-14
DE69225074T2 DE69225074T2 (de) 1998-10-29

Family

ID=9420171

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69225074T Expired - Lifetime DE69225074T2 (de) 1991-12-18 1992-12-18 Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen

Country Status (7)

Country Link
US (1) US5795715A (de)
EP (1) EP0618966B1 (de)
JP (1) JP3515108B2 (de)
AT (1) ATE164883T1 (de)
DE (1) DE69225074T2 (de)
FR (1) FR2685346B1 (de)
WO (1) WO1993012229A1 (de)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605712B1 (en) * 1990-12-20 2003-08-12 Arch Development Corporation Gene transcription and ionizing radiation: methods and compositions
FR2706465B1 (fr) * 1993-06-11 1995-08-25 Heliosynthese Sa Procédé de production et d'extraction de superoxyde-dismutases thermostables à partir d'une culture de micro-organismes photosynthétiques.
US6458940B2 (en) * 1995-06-06 2002-10-01 Hybridon, Inc. HPV-specific oligonucleotides
US20030055240A1 (en) * 1995-06-06 2003-03-20 Roberts Peter C. HPV specific oligonucleotides
US6509149B2 (en) 1995-06-06 2003-01-21 Hybridon, Inc. HPV-specific oligonucleotides
US6274369B1 (en) * 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
ATE526405T1 (de) 1998-03-20 2011-10-15 Commw Scient Ind Res Org Synthetische gene und genetische zusammensetzungen diesen enthaltend
EP2314700A1 (de) * 1999-01-28 2011-04-27 Medical College of Georgia Research Institute, Inc Zusammensetzung und Verfahren zur in vivo und in vitro Abschwächung der Genexpression mittels dobbelsträngiger RNA
KR20010112944A (ko) * 1999-04-21 2001-12-22 이곤 이 버그 폴리뉴클레오티드 서열의 기능을 억제하기 위한 방법 및조성물
US20040138168A1 (en) * 1999-04-21 2004-07-15 Wyeth Methods and compositions for inhibiting the function of polynucleotide sequences
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6423885B1 (en) * 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
WO2001029058A1 (en) * 1999-10-15 2001-04-26 University Of Massachusetts Rna interference pathway genes as tools for targeted genetic interference
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
KR20080023768A (ko) 2000-03-30 2008-03-14 화이트헤드 인스티튜트 포 바이오메디칼 리서치 Rna 간섭의 rna 서열 특이적인 매개체
US7176026B2 (en) 2001-11-09 2007-02-13 Basf Plant Science Gmbh Protein kinase stress-related polypeptides and methods of use in plants
US7700359B2 (en) * 2000-06-02 2010-04-20 Novartis Vaccines And Diagnostics, Inc. Gene products differentially expressed in cancerous cells
US6465217B1 (en) * 2000-07-05 2002-10-15 Paradigm Genetics, Inc. Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants
US20080242627A1 (en) * 2000-08-02 2008-10-02 University Of Southern California Novel rna interference methods using dna-rna duplex constructs
US20020173478A1 (en) * 2000-11-14 2002-11-21 The Trustees Of The University Of Pennsylvania Post-transcriptional gene silencing by RNAi in mammalian cells
KR100909681B1 (ko) * 2000-12-01 2009-07-29 막스-플랑크-게젤샤프트 츄어 푀르더룽 데어 비쎈샤프텐 에.파우. Rna 간섭을 매개하는 작은 rna 분자
CA2369944A1 (en) * 2001-01-31 2002-07-31 Nucleonics Inc. Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
US20040242523A1 (en) * 2003-03-06 2004-12-02 Ana-Farber Cancer Institue And The Univiersity Of Chicago Chemo-inducible cancer gene therapy
ES2380007T3 (es) * 2001-04-06 2012-05-07 The University Of Chicago Inducción por agentes quimioterapéuticos de la actividad del promotor Egr-1 en la terapia génica
US8034791B2 (en) 2001-04-06 2011-10-11 The University Of Chicago Activation of Egr-1 promoter by DNA damaging chemotherapeutics
AU2003303395A1 (en) * 2001-05-22 2004-07-22 Dahl, Gary, A. Target-dependent transcription using deletion mutants of n4 rna polymerase
AUPR621501A0 (en) 2001-07-06 2001-08-02 Commonwealth Scientific And Industrial Research Organisation Delivery of ds rna
TWI335938B (en) * 2001-08-15 2011-01-11 Rna replication and amplification
ATE510016T1 (de) 2001-11-09 2011-06-15 Basf Plant Science Gmbh Mit stress assoziierte transkriptionsfaktor- spezifische polypeptide und verfahren zur verwendung in pflanzen
AU2003235791A1 (en) * 2002-01-08 2003-07-24 Chiron Corporation Gene products differentially expressed in cancerous breast cells and their methods of use
WO2003064621A2 (en) * 2002-02-01 2003-08-07 Ambion, Inc. HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
CA2474910A1 (en) 2002-02-01 2003-08-07 Sequitur, Inc. Oligonucleotide compositions with enhanced efficiency
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
GB2406169B (en) * 2002-06-12 2006-11-01 Ambion Inc Methods and compositions relating to labeled rna molecules that reduce gene expression
US20040248094A1 (en) * 2002-06-12 2004-12-09 Ford Lance P. Methods and compositions relating to labeled RNA molecules that reduce gene expression
AU2003257934A1 (en) 2002-08-01 2004-02-23 City Of Hope Methods and kits for synthesis of sirna expression cassettes
US7829694B2 (en) * 2002-11-26 2010-11-09 Medtronic, Inc. Treatment of neurodegenerative disease through intracranial delivery of siRNA
US7618948B2 (en) 2002-11-26 2009-11-17 Medtronic, Inc. Devices, systems and methods for improving and/or cognitive function through brain delivery of siRNA
US6852494B2 (en) * 2003-01-10 2005-02-08 Linden Technologies, Inc. Nucleic acid amplification
US20050164212A1 (en) * 2003-03-06 2005-07-28 Todd Hauser Modulation of gene expression using DNA-RNA hybrids
WO2006032707A2 (en) 2004-09-24 2006-03-30 Basf Plant Science Gmbh Plant cells and plants with increased tolerance to environmental stress
AU2004230490C1 (en) 2003-04-15 2012-08-16 Basf Plant Science Gmbh Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress
US20050112128A1 (en) * 2003-05-21 2005-05-26 Myogen, Inc. And Board Of Regents, The University Of Texas System Inhibition of protein kinase c-mu (PKD) as a treatment for cardiac hypertrophy and heart failure
WO2005095622A2 (en) * 2004-03-26 2005-10-13 Van Andel Research Institute c-met siRNA ADENOVIRUS VECTORS INHIBIT CANCER CELL GROWTH, INVASION AND TUMORIGENICITY
WO2005103254A1 (en) * 2004-04-22 2005-11-03 Yissum Research Development Company Of The Hebrew University Of Jerusalem UNIVERSAL TARGET SEQUENCES FOR siRNA GENE SILENCING
NL1026335C2 (nl) * 2004-06-04 2005-12-06 Univ Delft Tech Werkwijze voor het maken van een dubbelstrengs polyribonucleotidenvolgorde met overhangend uiteinde, alsmede een werkwijze voor het vormen van een dubbelstrengs polynucleotidenconstruct en een toepassing.
AU2005286428B2 (en) 2004-09-24 2012-08-23 Basf Plant Science Gmbh Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress
US20060142228A1 (en) * 2004-12-23 2006-06-29 Ambion, Inc. Methods and compositions concerning siRNA's as mediators of RNA interference
DE202005004135U1 (de) * 2005-03-11 2005-05-19 Klocke Verpackungs-Service Gmbh Mehrkomponentenverpackung mit Applikator
EP2500429A3 (de) * 2005-05-31 2015-10-28 Devgen N.V. RNAi zur Kontrolle von Insekten und Spinnen
CN101203603B (zh) 2005-06-17 2011-11-16 巴斯福植物科学有限公司 凝集素样蛋白激酶胁迫相关多肽和在植物内使用的方法
EP1907552B1 (de) 2005-07-18 2013-10-23 BASF Plant Science GmbH Erhöhung des Erntevolumens bei Pflanzen durch Überexpression der ACCDP-Gene
AU2006270158C1 (en) 2005-07-18 2012-03-29 Basf Plant Science Gmbh Yield increase in plants overexpressing the SHSRP genes
CN101365794A (zh) 2005-08-12 2009-02-11 巴斯福植物科学有限公司 编码与非生物性胁迫应答相关的蛋白质的核酸序列和具有增加的环境胁迫耐受性的植物细胞及植物
WO2007047408A2 (en) * 2005-10-12 2007-04-26 Pathologica, Llc. Promac signature application
US8808698B2 (en) * 2006-02-03 2014-08-19 The Regents Of The University Of California Methods for inhibition of lymphangiogenesis and tumor metastasis
US20090172834A1 (en) 2006-03-24 2009-07-02 Basf Plant Science Gmbh Proteins Associated With Abiotic Stress Response And Homologs
HUE037173T2 (hu) 2006-08-08 2018-08-28 Univ Bonn Rheinische Friedrich Wilhelms 5'-Foszfát-oligonukleotidok szerkezete és alkalmazása
CN101589148B (zh) 2006-10-13 2014-07-02 巴斯福植物科学有限公司 产量提高的植物
CN101631868B (zh) 2007-02-16 2016-02-10 巴斯福植物科学有限公司 用于在单子叶植物中调节胚特异性表达的核酸序列
AR067318A1 (es) 2007-05-22 2009-10-07 Basf Plant Science Gmbh Plantas con mayor tolerancia y/o resistencia aumentada al estres ambiental y mayor produccion de biomasa
WO2008148068A1 (en) * 2007-05-23 2008-12-04 Mannkind Corporation Multicistronic vectors and methods for their design
US8664475B2 (en) 2007-09-18 2014-03-04 Basf Plant Science Gmbh Plants with increased yield
CN101939435A (zh) 2007-09-21 2011-01-05 巴斯夫植物科学有限公司 具有增加的产量的植物
EP2222857A2 (de) 2007-12-19 2010-09-01 BASF Plant Science GmbH Pflanzen mit erhöhtem ertrag und/oder erhöhter toleranz gegenüber umweltstress (iy-bm)
BRPI0907637A8 (pt) 2008-01-25 2018-01-02 P53 Inc biomarcadores p53
US8709393B2 (en) 2008-01-30 2014-04-29 Wound Engineering Llc Methods and compositions for wound healing
WO2009106596A2 (en) 2008-02-27 2009-09-03 Basf Plant Science Gmbh Plants with increased yield
US9738680B2 (en) 2008-05-21 2017-08-22 Rheinische Friedrich-Wilhelms-Universität Bonn 5′ triphosphate oligonucleotide with blunt end and uses thereof
AR074177A1 (es) 2008-08-19 2010-12-29 Basf Plant Science Gmbh Plantas con aumento de rendimiento al aumentar o generar una o mas actividades en una planta o parte de esta
US20110195843A1 (en) 2008-09-23 2011-08-11 Basf Plant Science Gmbh Plants with Increased Yield (LT)
EP2350287A2 (de) 2008-10-23 2011-08-03 BASF Plant Science GmbH Verfahren zur herstellung einer transgenen zelle mit erhöhtem gamma-aminobuttersäure-(gaba-)gehalt
DE112009002577T5 (de) 2008-10-23 2012-06-21 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag (NUE)
EP2186822A1 (de) 2008-11-07 2010-05-19 Sanofi-Aventis Verwendung von Inhibitoren der Plac8-Aktivität zur Modulation von Adipogenese
EP2236607A1 (de) 2009-04-02 2010-10-06 Sanofi-Aventis Verwendung von Hemmern der St3Gla6-Aktivität zur Adipogenesemodulation
EP2253705A1 (de) 2009-05-20 2010-11-24 Sanofi-Aventis Verwendung von Hemmern der Zdhhc2-Aktivität zur Adipogenesemodulation
DE112010003032T5 (de) 2009-07-23 2012-08-02 Basf Plant Science Company Gmbh Pflanzen mit erhöhtem Ertrag
US9409983B2 (en) 2009-07-23 2016-08-09 The Board Of Trustess Of The University Of Illinois Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases
EP2308892A1 (de) 2009-10-01 2011-04-13 Sanofi-Aventis Verwendung von siRNA gegen Sipa1l1 zur Reduzierung von Lipogenese
CN102770543A (zh) 2009-11-17 2012-11-07 巴斯夫植物科学有限公司 具有增加的产量的植物
CN102781517B (zh) 2010-02-01 2015-11-25 小利兰·斯坦福大学托管委员会 诊断和治疗非胰岛素依赖性糖尿病的方法
WO2013005211A2 (en) 2011-07-05 2013-01-10 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Boron complexing plant materials and uses thereof cross-reference to related applications
US10040853B2 (en) 2011-09-09 2018-08-07 Fred Hutchinson Cancer Research Center Methods and compositions involving NKG2D inhibitors and cancer
WO2014045126A2 (en) 2012-09-18 2014-03-27 Uti Limited Partnership Treatment of pain by inhibition of usp5 de-ubiquitinase
EP2712870A1 (de) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Neuartige RIG-I-Liganden und Herstellungsverfahren dafür
EP3995158A1 (de) 2012-11-06 2022-05-11 Imbed Biosciences, Inc. Verfahren und zusammensetzungen zur wundheilung
EP2961853B1 (de) 2013-02-28 2018-09-19 The Board of Regents of The University of Texas System Verfahren zur klassifizierung einer krebserkrankung als anfälligkeit für tmepai-gerichtete therapien und zur heilung solcher krebserkrankungen
CA2965327C (en) 2013-11-08 2023-05-09 The Board Of Regents Of The University Of Texas System Vh4 antibodies against gray matter neuron and astrocyte
WO2016025629A1 (en) 2014-08-12 2016-02-18 The Regents Of The University Of California Molecular composition for enhancing and rejuvenating maintenance and repair of mammalian tissues
US10278986B2 (en) 2014-08-14 2019-05-07 The Regents Of The University Of Colorado, A Body Corporate Antibody-siRNA conjugates and uses therefor
AU2016265948B2 (en) 2015-05-18 2018-12-06 Alkahest, Inc. Methods and compositions for treating aging-associated impairments
EP3144014A1 (de) 2015-09-21 2017-03-22 Commissariat A L'energie Atomique Et Aux Energies Alternatives Synthetische tödliche arzneimittelkombination zur behandlung von nierenzellenkarzinom
CN109843343A (zh) 2016-07-29 2019-06-04 因贝德生物科学公司 用于伤口愈合的方法和组合物
CA3053392A1 (en) 2017-02-28 2018-09-07 The Board Of Trustees Of The Leland Stanford Junior University Antifibrotic activity of cd47 blockade
EP3673066A4 (de) 2017-09-15 2021-05-26 Commonwealth Scientific and Industrial Research Organisation Rna-moleküle
CA3117112A1 (en) 2018-10-31 2020-05-07 Arizona Board Of Regents On Behalf Of The University Of Arizona Biomarkers and methods of use for radiation-induced lung injury
WO2022006286A1 (en) 2020-06-30 2022-01-06 Lunglife Ai Methods for detecting lung cancer
WO2023230531A1 (en) 2022-05-24 2023-11-30 Lunglife Ai, Inc. Methods for detecting circulating genetically abnormal cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766072A (en) * 1985-07-17 1988-08-23 Promega Corporation Vectors for in vitro production of RNA copies of either strand of a cloned DNA sequence
JPH04507083A (ja) * 1989-05-19 1992-12-10 ヘム・リサーチ・インコーポレーテッド 規定された構造の短い治療用dsRNA
EP0510085B1 (de) * 1990-01-10 1999-09-08 Chiron Diagnostics Corporation Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen

Also Published As

Publication number Publication date
US5795715A (en) 1998-08-18
EP0618966B1 (de) 1998-04-08
DE69225074T2 (de) 1998-10-29
EP0618966A1 (de) 1994-10-12
WO1993012229A1 (fr) 1993-06-24
JP3515108B2 (ja) 2004-04-05
JPH07505762A (ja) 1995-06-29
FR2685346B1 (fr) 1994-02-11
ATE164883T1 (de) 1998-04-15
FR2685346A1 (fr) 1993-06-25

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