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DE60326224D1 - Signaturen konstanter länge für das parallele sequenzieren von polynukleotiden - Google Patents

Signaturen konstanter länge für das parallele sequenzieren von polynukleotiden

Info

Publication number
DE60326224D1
DE60326224D1 DE60326224T DE60326224T DE60326224D1 DE 60326224 D1 DE60326224 D1 DE 60326224D1 DE 60326224 T DE60326224 T DE 60326224T DE 60326224 T DE60326224 T DE 60326224T DE 60326224 D1 DE60326224 D1 DE 60326224D1
Authority
DE
Germany
Prior art keywords
parallel sequencing
signatures
polynucleotides
constant length
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60326224T
Other languages
English (en)
Inventor
Achim Fischer
Holger Hiemisch
Steven Williams
Sydney Brenner
Roger Walker
Eric Vermaas
Rongdian Fu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Solexa Inc
Original Assignee
Solexa Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Solexa Inc filed Critical Solexa Inc
Application granted granted Critical
Publication of DE60326224D1 publication Critical patent/DE60326224D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60326224T 2002-04-26 2003-04-25 Signaturen konstanter länge für das parallele sequenzieren von polynukleotiden Expired - Lifetime DE60326224D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37578202P 2002-04-26 2002-04-26
PCT/US2003/013076 WO2003091416A2 (en) 2002-04-26 2003-04-25 Constant length signatures for parallel sequencing of polynucleotides

Publications (1)

Publication Number Publication Date
DE60326224D1 true DE60326224D1 (de) 2009-04-02

Family

ID=29270698

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60326224T Expired - Lifetime DE60326224D1 (de) 2002-04-26 2003-04-25 Signaturen konstanter länge für das parallele sequenzieren von polynukleotiden

Country Status (8)

Country Link
US (1) US20040002104A1 (de)
EP (1) EP1497465B1 (de)
JP (1) JP4446746B2 (de)
AT (1) ATE423224T1 (de)
AU (1) AU2003234255A1 (de)
CA (1) CA2482425A1 (de)
DE (1) DE60326224D1 (de)
WO (1) WO2003091416A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003238702A1 (en) * 2002-06-12 2003-12-31 Kabushiki Kaisha Dnaform Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
CN1747748B (zh) 2003-05-23 2011-01-19 尼克塔治疗公司 具有特定原子排列的聚合物衍生物
US7947261B2 (en) 2003-05-23 2011-05-24 Nektar Therapeutics Conjugates formed from polymer derivatives having particular atom arrangements
US20060228714A1 (en) * 2004-02-17 2006-10-12 Dana Farber Cancer Institute Nucleic acid representations utilizing type IIB restriction endonuclease cleavage products
EP1582599A1 (de) * 2004-03-31 2005-10-05 Takara Bio Inc. Verfahren zur Mikroperlenreinigung
JP4644685B2 (ja) * 2004-07-02 2011-03-02 株式会社ダナフォーム 塩基配列タグの調製方法
EP2395113A1 (de) 2007-06-29 2011-12-14 Population Genetics Technologies Ltd. Verfahren und Zusammensetzungen zur Isolierung von Nukleinsäuresequenzvarianten
EP3448868B1 (de) * 2016-04-27 2021-12-29 Bio-Rad Laboratories, Inc. Verfahren für mirna-transkriptom und zusammensetzungen
CN109477142B (zh) * 2016-07-18 2022-03-22 豪夫迈·罗氏有限公司 不对称模板和核酸测序的不对称方法
CN108410856A (zh) * 2018-03-29 2018-08-17 武汉光谷创赢生物技术开发有限公司 一种全长cDNA合成方法及其测序文库的构建

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5093245A (en) * 1988-01-26 1992-03-03 Applied Biosystems Labeling by simultaneous ligation and restriction
US6107023A (en) * 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5629179A (en) * 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
US5866330A (en) * 1995-09-12 1999-02-02 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US5695937A (en) * 1995-09-12 1997-12-09 The Johns Hopkins University School Of Medicine Method for serial analysis of gene expression
US6461814B1 (en) * 1997-01-15 2002-10-08 Dominic G. Spinella Method of identifying gene transcription patterns
US6232067B1 (en) * 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
AU6770800A (en) * 1999-08-13 2001-03-13 Yale University Analysis of sequence tags with hairpin primers
US6274320B1 (en) * 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid

Also Published As

Publication number Publication date
JP2006506953A (ja) 2006-03-02
EP1497465A2 (de) 2005-01-19
WO2003091416A3 (en) 2004-07-01
JP4446746B2 (ja) 2010-04-07
WO2003091416A2 (en) 2003-11-06
EP1497465A4 (de) 2005-06-08
EP1497465B1 (de) 2009-02-18
ATE423224T1 (de) 2009-03-15
US20040002104A1 (en) 2004-01-01
AU2003234255A1 (en) 2003-11-10
CA2482425A1 (en) 2003-11-06

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