DE60316642D1 - Verbessertes Verfahren zur Bisulfit-Behandlung - Google Patents
Verbessertes Verfahren zur Bisulfit-BehandlungInfo
- Publication number
- DE60316642D1 DE60316642D1 DE60316642T DE60316642T DE60316642D1 DE 60316642 D1 DE60316642 D1 DE 60316642D1 DE 60316642 T DE60316642 T DE 60316642T DE 60316642 T DE60316642 T DE 60316642T DE 60316642 D1 DE60316642 D1 DE 60316642D1
- Authority
- DE
- Germany
- Prior art keywords
- solid phase
- glass
- nucleic acid
- bisulfite
- improved process
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 239000011521 glass Substances 0.000 abstract 4
- 239000007790 solid phase Substances 0.000 abstract 4
- 238000006243 chemical reaction Methods 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 2
- 230000009615 deamination Effects 0.000 abstract 2
- 238000006481 deamination reaction Methods 0.000 abstract 2
- 230000006326 desulfonation Effects 0.000 abstract 2
- 238000005869 desulfonation reaction Methods 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 230000011987 methylation Effects 0.000 abstract 1
- 238000007069 methylation reaction Methods 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 239000000377 silicon dioxide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Compounds Of Iron (AREA)
- Saccharide Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02019097 | 2002-08-29 | ||
| EP02019097A EP1394172A1 (de) | 2002-08-29 | 2002-08-29 | Verbessertes Verfahren Zur Bisulfit-Behandlung |
| EP02028114 | 2002-12-18 | ||
| EP02028114 | 2002-12-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60316642D1 true DE60316642D1 (de) | 2007-11-15 |
| DE60316642T2 DE60316642T2 (de) | 2008-01-31 |
Family
ID=32071078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60316642T Expired - Lifetime DE60316642T2 (de) | 2002-08-29 | 2003-08-27 | Verbessertes Verfahren zur Bisulfit-Behandlung |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US9394332B2 (de) |
| EP (2) | EP1829886B1 (de) |
| JP (2) | JP5153048B2 (de) |
| CN (1) | CN1271081C (de) |
| AT (1) | ATE374782T1 (de) |
| AU (1) | AU2003236461B2 (de) |
| CA (1) | CA2438327C (de) |
| CY (1) | CY1107118T1 (de) |
| DE (1) | DE60316642T2 (de) |
| DK (2) | DK1829886T3 (de) |
| ES (2) | ES2545577T3 (de) |
| HU (1) | HUE027240T2 (de) |
| PT (1) | PT1394173E (de) |
| SI (2) | SI1394173T1 (de) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10154317B4 (de) * | 2001-10-26 | 2005-06-09 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben |
| US9394332B2 (en) | 2002-08-29 | 2016-07-19 | Epigenomics Ag | Method for bisulfite treatment |
| US7262013B2 (en) * | 2003-08-29 | 2007-08-28 | Applera Corporation | Bisulfite method |
| US7534873B2 (en) * | 2003-08-29 | 2009-05-19 | Applied Biosystems, Llc | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| US7368239B2 (en) * | 2003-08-29 | 2008-05-06 | Applera Corporation | Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil |
| US7371526B2 (en) * | 2003-08-29 | 2008-05-13 | Applera Corporation | Method and materials for bisulfite conversion of cytosine to uracil |
| CA2540310A1 (en) | 2003-10-09 | 2005-04-28 | Epigenomics Ag | Improved bisulfite conversion of dna |
| JPWO2005100240A1 (ja) * | 2004-04-08 | 2008-03-06 | 東洋紡績株式会社 | Ｄｎａの脱アミノ化のための組成物及びメチル化ｄｎａの検出方法 |
| EP1632578A1 (de) | 2004-09-03 | 2006-03-08 | Roche Diagnostics GmbH | Methode zur Dekontamination der DNA |
| JP2008515784A (ja) * | 2004-09-21 | 2008-05-15 | アプレラ コーポレイション | メチル化ｄｎａ分析のための硫酸水素ナトリウムの改良代替物として硫黄求核剤を使用する方法 |
| US7658288B2 (en) | 2004-11-08 | 2010-02-09 | Applied Biosystems, Llc | Bisulfite conversion reagent |
| ES2647483T3 (es) | 2005-04-01 | 2017-12-21 | Epigenomics Ag | Conversión por bisulfito mejorada de ADN |
| EP1963352B1 (de) * | 2005-12-14 | 2010-09-08 | Roche Diagnostics GmbH | Neues verfahren für die bisulfitbehandlung |
| US7820385B2 (en) * | 2006-03-22 | 2010-10-26 | The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention | Method for retaining methylation pattern in globally amplified DNA |
| US20080213870A1 (en) * | 2007-03-01 | 2008-09-04 | Sean Wuxiong Cao | Methods for obtaining modified DNA from a biological specimen |
| EP2240603A4 (de) * | 2008-01-03 | 2011-07-20 | Univ Johns Hopkins | Zusammensetzungen und verfahren für polynukleotidextraktion und methylierungsnachweis |
| WO2010037001A2 (en) | 2008-09-26 | 2010-04-01 | Immune Disease Institute, Inc. | Selective oxidation of 5-methylcytosine by tet-family proteins |
| FR2940805B1 (fr) | 2009-01-05 | 2015-10-16 | Biomerieux Sa | Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede |
| JP6138118B2 (ja) | 2011-07-29 | 2017-05-31 | ケンブリッジ エピジェネティクス リミテッド | ヌクレオチド修飾の検出方法 |
| ES2669512T3 (es) | 2012-11-30 | 2018-05-28 | Cambridge Epigenetix Limited | Agente oxidante para nucleótidos modificados |
| WO2016198582A1 (en) * | 2015-06-10 | 2016-12-15 | Biocartis N.V. | Improved detection of methylated dna |
| EP3839047A1 (de) * | 2015-06-15 | 2021-06-23 | Cepheid | Integrierte reinigung und messung der dna-methylierung und ko-messung von mutationen und/oder mrna-expressionsniveaus in einer automatisierten reaktionskartusche |
| EP3551332B1 (de) | 2016-12-12 | 2024-01-10 | Cepheid | Integrierte reinigung und messung der dna-methylierung und ko-messung von mutationen und/oder mrna-expressionsniveaus in einer automatisierten reaktionskartusche |
| CN108265050A (zh) * | 2018-03-13 | 2018-07-10 | 普迈德（北京）科技有限公司 | 一种血浆直接亚硫酸氢盐转化的方法及其应用 |
| WO2019178277A1 (en) | 2018-03-13 | 2019-09-19 | Grail, Inc. | Anomalous fragment detection and classification |
| DE202019005627U1 (de) | 2018-04-02 | 2021-05-31 | Grail, Inc. | Methylierungsmarker und gezielte Methylierungssondenpanels |
| AU2019351130B2 (en) | 2018-09-27 | 2025-10-23 | GRAIL, Inc | Methylation markers and targeted methylation probe panel |
| WO2020132572A1 (en) | 2018-12-21 | 2020-06-25 | Grail, Inc. | Source of origin deconvolution based on methylation fragments in cell-free-dna samples |
| DE102019118332B4 (de) | 2019-07-07 | 2022-04-07 | Ist Innuscreen Gmbh | Verfahren und testkit zur bisulfitmodifizierung von dna |
| EP4096694A4 (de) | 2020-01-30 | 2024-01-24 | Prognomiq Inc | Lungenbiomarker und verfahren zur verwendung davon |
| CN116157519A (zh) * | 2020-07-29 | 2023-05-23 | 精密科学公司 | 经磺化dna的纯化 |
| US12334190B2 (en) | 2021-03-31 | 2025-06-17 | PrognomIQ, Inc. | Multi-omic assessment using proteins and nucleic acids |
| US12387508B2 (en) | 2021-09-10 | 2025-08-12 | PrognomIQ, Inc. | Direct classification of raw biomolecule measurement data |
| EP4402473A4 (de) | 2021-09-13 | 2025-07-30 | Prognomiq Inc | Verbesserter nachweis und quantifizierung von biomolekülen |
| CN114317761A (zh) * | 2022-02-23 | 2022-04-12 | 厦门飞朔生物技术有限公司 | 一种宫颈癌相关基因甲基化检测方法 |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DK171161B1 (da) | 1985-03-28 | 1996-07-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve |
| DE3724442A1 (de) | 1987-07-23 | 1989-02-02 | Europ Lab Molekularbiolog | Verfahren und vorrichtung zur reinigung von m-13-phagen-dna |
| IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| EP0425563B1 (de) | 1988-07-20 | 1996-05-15 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| WO1990006045A2 (en) | 1988-11-21 | 1990-06-14 | Dynal As | Nucleic acid probes |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| CA2102264C (en) | 1992-11-13 | 2000-08-01 | Daniel Lee Woodard | Boron silicates, aluminum silicates, phosphosilicates and purification of dna |
| US5503816A (en) | 1993-09-27 | 1996-04-02 | Becton Dickinson And Company | Silicate compounds for DNA purification |
| US5438127A (en) | 1993-09-27 | 1995-08-01 | Becton Dickinson And Company | DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane |
| US5552277A (en) | 1994-07-19 | 1996-09-03 | The Johns Hopkins University School Of Medicine | Genetic diagnosis of prostate cancer |
| WO2000032762A1 (de) | 1998-11-30 | 2000-06-08 | Roche Diagnostics Gmbh | Magnetische partikel zur reinigung von nukleinsäuren |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| US5786146A (en) | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| DE69739357D1 (de) | 1996-06-04 | 2009-05-28 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| AU745126B2 (en) * | 1997-04-16 | 2002-03-14 | Applied Biosystems, Llc | Nucleic acid archiving |
| GB9714716D0 (en) * | 1997-07-11 | 1997-09-17 | Brax Genomics Ltd | Characterising nucleic acids |
| DE19743518A1 (de) | 1997-10-01 | 1999-04-15 | Roche Diagnostics Gmbh | Automatisierbare universell anwendbare Probenvorbereitungsmethode |
| DE59912604D1 (de) | 1998-02-04 | 2005-11-03 | Merck Patent Gmbh | Verfahren zur isolierung und aufreinigung von nucleinsäuren |
| DE19856064C2 (de) | 1998-12-04 | 2000-11-30 | Invitek Gmbh | Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien |
| US6331393B1 (en) | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| US6423536B1 (en) | 1999-08-02 | 2002-07-23 | Molecular Dynamics, Inc. | Low volume chemical and biochemical reaction system |
| ES2256068T3 (es) * | 1999-11-17 | 2006-07-16 | Roche Diagnostics Gmbh | Particulas de cristal magneticas, metodo para su preparacion y usos de las mismas. |
| DE19958042A1 (de) | 1999-12-03 | 2001-06-21 | Invitek Gmbh | Oberflächenmodifizierte Trägermaterialien zur Bindung biologischer Materialien, Verfahren zu ihrer Herstellung und ihre Verwendung |
| DE10029915B4 (de) | 2000-06-19 | 2005-06-16 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| DE10050942B4 (de) | 2000-10-10 | 2005-11-17 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| US20030152950A1 (en) | 2001-06-27 | 2003-08-14 | Garner Harold R. | Identification of chemically modified polymers |
| WO2003012124A2 (en) * | 2001-07-31 | 2003-02-13 | New York Institute Of Technology | Methods for immobilizing molecules to a solid phase and uses thereof |
| DE10154317B4 (de) * | 2001-10-26 | 2005-06-09 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben |
| US9394332B2 (en) | 2002-08-29 | 2016-07-19 | Epigenomics Ag | Method for bisulfite treatment |
| EP1394172A1 (de) | 2002-08-29 | 2004-03-03 | Boehringer Mannheim Gmbh | Verbessertes Verfahren Zur Bisulfit-Behandlung |
| JP4435174B2 (ja) * | 2003-12-02 | 2010-03-17 | エフ．ホフマン−ラ ロシュ アーゲー | 重亜硫酸塩処理の改良された方法 |
| EP1963352B1 (de) * | 2005-12-14 | 2010-09-08 | Roche Diagnostics GmbH | Neues verfahren für die bisulfitbehandlung |
-
2003
- 2003-08-25 US US10/647,720 patent/US9394332B2/en active Active
- 2003-08-25 AU AU2003236461A patent/AU2003236461B2/en not_active Ceased
- 2003-08-27 SI SI200331015T patent/SI1394173T1/sl unknown
- 2003-08-27 ES ES07012188.4T patent/ES2545577T3/es not_active Expired - Lifetime
- 2003-08-27 DK DK07012188.4T patent/DK1829886T3/en active
- 2003-08-27 EP EP07012188.4A patent/EP1829886B1/de not_active Revoked
- 2003-08-27 PT PT03019321T patent/PT1394173E/pt unknown
- 2003-08-27 DE DE60316642T patent/DE60316642T2/de not_active Expired - Lifetime
- 2003-08-27 ES ES03019321T patent/ES2294231T3/es not_active Expired - Lifetime
- 2003-08-27 HU HUE07012188A patent/HUE027240T2/en unknown
- 2003-08-27 SI SI200332443T patent/SI1829886T1/sl unknown
- 2003-08-27 AT AT03019321T patent/ATE374782T1/de active
- 2003-08-27 DK DK03019321T patent/DK1394173T3/da active
- 2003-08-27 EP EP03019321A patent/EP1394173B9/de not_active Expired - Lifetime
- 2003-08-28 CN CNB031602835A patent/CN1271081C/zh not_active Expired - Fee Related
- 2003-08-28 CA CA002438327A patent/CA2438327C/en not_active Expired - Fee Related
- 2003-08-29 JP JP2003306141A patent/JP5153048B2/ja not_active Expired - Lifetime
-
2008
- 2008-01-02 CY CY20081100002T patent/CY1107118T1/el unknown
-
2009
- 2009-02-13 JP JP2009031400A patent/JP5310059B2/ja not_active Expired - Lifetime
-
2015
- 2015-08-24 US US14/833,738 patent/US9868756B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DK1829886T3 (en) | 2015-08-17 |
| ES2294231T3 (es) | 2008-04-01 |
| PT1394173E (pt) | 2007-11-28 |
| HUE027240T2 (en) | 2016-10-28 |
| EP1829886A2 (de) | 2007-09-05 |
| EP1829886B1 (de) | 2015-07-29 |
| CA2438327A1 (en) | 2004-02-29 |
| US9394332B2 (en) | 2016-07-19 |
| CY1107118T1 (el) | 2012-10-24 |
| EP1829886A3 (de) | 2008-10-15 |
| ES2545577T3 (es) | 2015-09-14 |
| CN1495192A (zh) | 2004-05-12 |
| DE60316642T2 (de) | 2008-01-31 |
| SI1394173T1 (sl) | 2008-02-29 |
| AU2003236461A1 (en) | 2004-03-18 |
| EP1394173B9 (de) | 2008-10-15 |
| DK1394173T3 (da) | 2008-02-04 |
| JP5310059B2 (ja) | 2013-10-09 |
| EP1394173A1 (de) | 2004-03-03 |
| ATE374782T1 (de) | 2007-10-15 |
| JP2004089195A (ja) | 2004-03-25 |
| JP2009106304A (ja) | 2009-05-21 |
| SI1829886T1 (sl) | 2015-12-31 |
| JP5153048B2 (ja) | 2013-02-27 |
| EP1394173B1 (de) | 2007-10-03 |
| US20160068888A1 (en) | 2016-03-10 |
| CN1271081C (zh) | 2006-08-23 |
| HK1062688A1 (en) | 2004-11-19 |
| US9868756B2 (en) | 2018-01-16 |
| CA2438327C (en) | 2008-04-15 |
| AU2003236461B2 (en) | 2009-05-28 |
| US20040241704A1 (en) | 2004-12-02 |
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