DE3720844C2 - - Google Patents

Info

Publication number: DE3720844C2
Authority: DE; Germany
Prior art keywords: separation column; plastic; separation; magnetic; column according
Prior art date: 1987-06-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

DE19873720844

Other languages

German (de)

English (en)

Other versions

DE3720844A1 (de

Inventor

Stefan 5060 Bergisch Gladbach De Miltenyi

Andreas Dr. 5300 Bonn De Radbruch

Walter Weichel

Werner Dr. Mueller

Christoph 5000 Koeln De Goettlinger

Klaus Ludwig 5024 Pulheim De Meyer

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Miltenyi Biotec GmbH

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-06-24

Filing date

1987-06-24

Publication date

1991-01-10

1987-06-24 Application filed by Individual filed Critical Individual

1987-06-24 Priority to DE19873720844 priority Critical patent/DE3720844A1/de

1989-01-05 Publication of DE3720844A1 publication Critical patent/DE3720844A1/de

1991-01-10 Application granted granted Critical

1991-01-10 Publication of DE3720844C2 publication Critical patent/DE3720844C2/de

Status Granted legal-status Critical Current

Links

238000000926 separation method Methods 0.000 claims description 29
230000005291 magnetic effect Effects 0.000 claims description 24
239000004033 plastic Substances 0.000 claims description 10
230000005294 ferromagnetic effect Effects 0.000 claims description 9
239000011248 coating agent Substances 0.000 claims description 5
238000000576 coating method Methods 0.000 claims description 5
238000004519 manufacturing process Methods 0.000 claims description 5
239000000126 substance Substances 0.000 claims description 5
239000003302 ferromagnetic material Substances 0.000 claims description 4
239000000835 fiber Substances 0.000 claims description 4
239000007788 liquid Substances 0.000 claims description 4
238000010584 magnetic trap Methods 0.000 claims description 3
238000007885 magnetic separation Methods 0.000 claims description 2
230000001413 cellular effect Effects 0.000 claims 1
238000012856 packing Methods 0.000 claims 1
238000009736 wetting Methods 0.000 claims 1
210000004027 cell Anatomy 0.000 description 12
239000002245 particle Substances 0.000 description 12
238000000034 method Methods 0.000 description 7
239000011159 matrix material Substances 0.000 description 5
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
229910000831 Steel Inorganic materials 0.000 description 4
210000003850 cellular structure Anatomy 0.000 description 4
239000010959 steel Substances 0.000 description 4
210000002268 wool Anatomy 0.000 description 4
230000006378 damage Effects 0.000 description 3
102000008857 Ferritin Human genes 0.000 description 2
238000008416 Ferritin Methods 0.000 description 2
108050000784 Ferritin Proteins 0.000 description 2
230000007797 corrosion Effects 0.000 description 2
238000005260 corrosion Methods 0.000 description 2
230000008878 coupling Effects 0.000 description 2
238000010168 coupling process Methods 0.000 description 2
238000005859 coupling reaction Methods 0.000 description 2
210000003743 erythrocyte Anatomy 0.000 description 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
150000002500 ions Chemical class 0.000 description 2
239000000463 material Substances 0.000 description 2
239000002184 metal Substances 0.000 description 2
229910052751 metal Inorganic materials 0.000 description 2
239000000203 mixture Substances 0.000 description 2
230000005298 paramagnetic effect Effects 0.000 description 2
229910001220 stainless steel Inorganic materials 0.000 description 2
231100000331 toxic Toxicity 0.000 description 2
230000002588 toxic effect Effects 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
102000001554 Hemoglobins Human genes 0.000 description 1
108010054147 Hemoglobins Proteins 0.000 description 1
102000004877 Insulin Human genes 0.000 description 1
108090001061 Insulin Proteins 0.000 description 1
229920000297 Rayon Polymers 0.000 description 1
208000027418 Wounds and injury Diseases 0.000 description 1
238000005266 casting Methods 0.000 description 1
230000005779 cell damage Effects 0.000 description 1
208000037887 cell injury Diseases 0.000 description 1
210000000170 cell membrane Anatomy 0.000 description 1
239000000919 ceramic Substances 0.000 description 1
239000000470 constituent Substances 0.000 description 1
230000000694 effects Effects 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
208000014674 injury Diseases 0.000 description 1
229910052500 inorganic mineral Inorganic materials 0.000 description 1
229940125396 insulin Drugs 0.000 description 1
229910052742 iron Inorganic materials 0.000 description 1
238000005304 joining Methods 0.000 description 1
239000004922 lacquer Substances 0.000 description 1
210000004698 lymphocyte Anatomy 0.000 description 1
239000006249 magnetic particle Substances 0.000 description 1
210000004962 mammalian cell Anatomy 0.000 description 1
239000003550 marker Substances 0.000 description 1
QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
229910052753 mercury Inorganic materials 0.000 description 1
239000011859 microparticle Substances 0.000 description 1
239000011707 mineral Substances 0.000 description 1
210000003463 organelle Anatomy 0.000 description 1
239000003973 paint Substances 0.000 description 1
238000004382 potting Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
239000010935 stainless steel Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
239000002966 varnish Substances 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
- B01J20/28023—Fibres or filaments
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D43/00—Separating particles from liquids, or liquids from solids, otherwise than by sedimentation or filtration
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28009—Magnetic properties
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/034—Component parts; Auxiliary operations characterised by the magnetic circuit characterised by the matrix elements
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Immunology (AREA)
Biomedical Technology (AREA)
Analytical Chemistry (AREA)
Molecular Biology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Chemical Kinetics & Catalysis (AREA)
Biochemistry (AREA)
Cell Biology (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Wood Science & Technology (AREA)
Food Science & Technology (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Physics & Mathematics (AREA)
Sustainable Development (AREA)
Tropical Medicine & Parasitology (AREA)
General Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

DE19873720844 1987-06-24 1987-06-24 Trennsaeule fuer die magnetische separierung von zellen, zellaggregaten, und zellulaeren bestandteilen Granted DE3720844A1 (de)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
DE19873720844 DE3720844A1 (de)	1987-06-24	1987-06-24	Trennsaeule fuer die magnetische separierung von zellen, zellaggregaten, und zellulaeren bestandteilen

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19873720844 DE3720844A1 (de)	1987-06-24	1987-06-24	Trennsaeule fuer die magnetische separierung von zellen, zellaggregaten, und zellulaeren bestandteilen

Publications (2)

Publication Number	Publication Date
DE3720844A1 DE3720844A1 (de)	1989-01-05
DE3720844C2 true DE3720844C2 (fr)	1991-01-10

Family

ID=6330187

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE19873720844 Granted DE3720844A1 (de)	1987-06-24	1987-06-24	Trennsaeule fuer die magnetische separierung von zellen, zellaggregaten, und zellulaeren bestandteilen

Country Status (1)

Country	Link
DE (1)	DE3720844A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE10144291A1 (de) *	2001-09-08	2003-03-27	Stefan Schuetze	Vorrichtung zur Isolierung von an magnetischen Partikeln gebundenem biologischen Material in einem Magnetfeld
EP2444158A2 (fr)	2010-10-20	2012-04-25	Miltenyi Biotec GmbH	Dispositif pour fragmenter un tissu
US9068991B2 (en)	2009-06-08	2015-06-30	Singulex, Inc.	Highly sensitive biomarker panels
US9239284B2 (en)	2007-12-19	2016-01-19	Singulex, Inc.	Scanning analyzer for single molecule detection and methods of use

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6020210A (en) *	1988-12-28	2000-02-01	Miltenvi Biotech Gmbh	Methods and materials for high gradient magnetic separation of biological materials
US5439586A (en) *	1993-09-15	1995-08-08	The Terry Fox Laboratory Of The British Columbia Cancer Agnecy	Magnetic filter with ordered wire array
US5503801A (en) *	1993-11-29	1996-04-02	Cobe Laboratories, Inc.	Top flow bubble trap apparatus
US5591251A (en) *	1993-11-29	1997-01-07	Cobe Laboratories, Inc.	Side flow bubble trap apparatus and method
US5705059A (en)	1995-02-27	1998-01-06	Miltenyi; Stefan	Magnetic separation apparatus
US6190870B1 (en) *	1995-08-28	2001-02-20	Amcell Corporation	Efficient enrichment and detection of disseminated tumor cells
US8685711B2 (en)	2004-09-28	2014-04-01	Singulex, Inc.	Methods and compositions for highly sensitive detection of molecules
US9040305B2 (en)	2004-09-28	2015-05-26	Singulex, Inc.	Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
WO2011140484A1 (fr)	2010-05-06	2011-11-10	Singulex, Inc	Méthodes de diagnostic, de classification et de prédiction du risque de développement d'une polyarthrite rhumatoïde et identification des sujets répondant à un traitement
DE102021214281A1 (de) *	2021-12-14	2023-06-15	Robert Bosch Gesellschaft mit beschränkter Haftung	Vorrichtung und Verfahren zum Splitten dreidimensionaler Agglomerate

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE734137C (de) *	1938-09-21	1943-04-08	Deutsche Edelstahlwerke Ag	Dauermagnetfilter zum Abscheiden magnetisierbarer Stoffe aus stroemenden Fluessigkeiten
US3700555A (en) *	1970-10-12	1972-10-24	Technicon Instr	Method and apparatus for lymphocyte separation from blood
DE2616734A1 (de) *	1975-04-16	1976-10-28	English Clays Lovering Pochin	Verfahren und vorrichtung zum modifizieren der effektiven groesse von teilchen von verschiedener magnetischer suszeptibilitaet, die miteinander vermischt und in einem fluid suspendiert sind
US4508625A (en) *	1982-10-18	1985-04-02	Graham Marshall D	Magnetic separation using chelated magnetic ions

1987
- 1987-06-24 DE DE19873720844 patent/DE3720844A1/de active Granted

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE10144291A1 (de) *	2001-09-08	2003-03-27	Stefan Schuetze	Vorrichtung zur Isolierung von an magnetischen Partikeln gebundenem biologischen Material in einem Magnetfeld
DE10144291C2 (de) *	2001-09-08	2003-10-09	Stefan Schuetze	Vorrichtung zur Isolierung von an magnetischen Partikeln gebundenem biologischen Material in einem Magnetfeld
US9239284B2 (en)	2007-12-19	2016-01-19	Singulex, Inc.	Scanning analyzer for single molecule detection and methods of use
US9068991B2 (en)	2009-06-08	2015-06-30	Singulex, Inc.	Highly sensitive biomarker panels
EP2444158A2 (fr)	2010-10-20	2012-04-25	Miltenyi Biotec GmbH	Dispositif pour fragmenter un tissu
DE102010042723A1 (de)	2010-10-20	2012-04-26	Miltenyi Biotec Gmbh	Vorrichtung und Verfahren zur Separation von Neél- und Brown-magnetischen Partikeln

Also Published As

Publication number	Publication date
DE3720844A1 (de)	1989-01-05

Publication	Publication Date	Title
DE3720844C2 (fr)	1991-01-10
DE69628584T2 (de)	2004-05-13	Vorrichtung zur magnetischen trennung
DE68919715T2 (de)	1995-04-06	Verfahren sowie materialien zur hochgraduierten magnetischen abspaltung biologischer materialien.
DE69520284T2 (de)	2002-08-01	Mikropartikel-trennvorrichtung mit einem magnetischen stab
DE69522461T2 (de)	2002-05-23	Vorrichtung zum transfer magnetischer teilchen
EP3286312B1 (fr)	2022-07-20	Procédé et dispositif d'extraction d'acides nucléiques
WO2009033947A2 (fr)	2009-03-19	Séparation magnétique à haut gradient de champ pour matériau biologique
EP0206077A2 (fr)	1986-12-30	Appareil de séparation pour particules magnétiques en phase liquide
DE3390261T1 (de)	1985-01-10	Verfahren zur magnetischen Abtrennung von Teilchen von einem Trägermedium unter Verwendung chelatgebundener magnetischer Ionen
Cook	1994	Hypothesis: RNA polymerase: Structural determinat of the chromatin loop and the chromosome
EP0687505A1 (fr)	1995-12-20	Méthode de séparation magnétique pour des composants d'un liquide
EP1333932A1 (fr)	2003-08-13	Procede de separation d'un produit disperse ou dissous et separateur magnetique y relatif
DE102017204267A1 (de)	2018-09-20	Verfahren zur anreicherung von zellen aus einer probe und der nachfolgenden nukleinsäureisolierung aus diesen zellen
EP2192987B1 (fr)	2020-04-22	Dispositif et procédé de traitement de liquides avec des particules magnétiques
DE102012210457B4 (de)	2015-08-27	Verfahren und Anordnung zur partiellen Markierung und anschließenden Quantifizierung von Zellen einer Zellsuspension
DE3003755C2 (fr)	1988-11-24
EP0184710A2 (fr)	1986-06-18	Microsphères magnétiques
DE3624626A1 (de)	1988-01-28	Verfahren zur abtrennung von stoffen aus einem stoffgemisch unter verwendung von magnetischen fluessigkeiten
DE2631610C2 (de)	1985-12-05	Verfahren zum Nachweis von Hepatitis in Seren
DE102005034327B3 (de)	2006-11-30	Vorrichtung zur Affinitätsseparation mittels magnetischer Partikel
DE60206056T2 (de)	2006-06-22	Herstellung von polymerteilchen
DE4416784A1 (de)	1995-11-30	Methode zur Anreicherung von Zellen, die durch ballistischen Transfer modifiziert wurden
EP2872618B1 (fr)	2018-12-19	Procédé pour le déplacement contrôlé de cellules mobiles dans les milieux liquides ou gazeux
DE102015013851B3 (de)	2016-10-13	Methode zur verbesserten magnetischen Markierung von biologischen Materialien
DE10053553A1 (de)	2002-05-23	Vorrichtung zum Nachweis und zum Anreichern von Biomolekülen

Legal Events

Date	Code	Title	Description
1989-01-05	OM8	Search report available as to paragraph 43 lit. 1 sentence 1 patent law
1989-01-05	OP8	Request for examination as to paragraph 44 patent law
1991-01-10	D2	Grant after examination
1991-07-04	8364	No opposition during term of opposition
1994-10-06	8327	Change in the person/name/address of the patent owner	Owner name: MILTENYI, STEFAN, 51429 BERGISCH GLADBACH, DE
1994-10-06	8381	Inventor (new situation)	Free format text: MILTENYI, STEFAN, 51429 BERGISCH GLADBACH, DE RADBRUCH, ANDREAS, DR., 53123 BONN, DE WEICHEL, WALTER, 51105 KOELN, DE MUELLER, WERNER, DR., 50931 KOELN, DE GOETTLINGER, CHRISTOPH, 50829 KOELN, DE MEYER, KLAUS LUDWIG, 50259 PULHEIM, DE
2000-08-03	8327	Change in the person/name/address of the patent owner	Owner name: MILTENYI BIOTEC GMBH, 51429 BERGISCH GLADBACH, DE