DE19926985B4 - Verfahren und Vorrichtung zur Gelelektrophorese - Google Patents
Verfahren und Vorrichtung zur Gelelektrophorese Download PDFInfo
- Publication number
- DE19926985B4 DE19926985B4 DE1999126985 DE19926985A DE19926985B4 DE 19926985 B4 DE19926985 B4 DE 19926985B4 DE 1999126985 DE1999126985 DE 1999126985 DE 19926985 A DE19926985 A DE 19926985A DE 19926985 B4 DE19926985 B4 DE 19926985B4
- Authority
- DE
- Germany
- Prior art keywords
- gel
- electrodes
- samples
- sample
- separating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001502 gel electrophoresis Methods 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000005684 electric field Effects 0.000 claims abstract description 12
- 238000000926 separation method Methods 0.000 claims description 27
- 239000000872 buffer Substances 0.000 claims description 25
- 239000011159 matrix material Substances 0.000 claims description 23
- 230000000694 effects Effects 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 2
- 239000013598 vector Substances 0.000 claims description 2
- 239000000499 gel Substances 0.000 description 86
- 239000000523 sample Substances 0.000 description 62
- 238000001962 electrophoresis Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 238000013508 migration Methods 0.000 description 4
- 230000005012 migration Effects 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012305 analytical separation technique Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- DBABZHXKTCFAPX-UHFFFAOYSA-N probenecid Chemical compound CCCN(CCC)S(=O)(=O)C1=CC=C(C(O)=O)C=C1 DBABZHXKTCFAPX-UHFFFAOYSA-N 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000012146 running buffer Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE1999126985 DE19926985B4 (de) | 1999-06-14 | 1999-06-14 | Verfahren und Vorrichtung zur Gelelektrophorese |
| PCT/EP2000/005472 WO2000077510A2 (fr) | 1999-06-14 | 2000-06-14 | Procede et dispositif pour electrophorese en gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE1999126985 DE19926985B4 (de) | 1999-06-14 | 1999-06-14 | Verfahren und Vorrichtung zur Gelelektrophorese |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE19926985A1 DE19926985A1 (de) | 2001-01-11 |
| DE19926985B4 true DE19926985B4 (de) | 2004-12-02 |
Family
ID=7911122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE1999126985 Expired - Fee Related DE19926985B4 (de) | 1999-06-14 | 1999-06-14 | Verfahren und Vorrichtung zur Gelelektrophorese |
Country Status (2)
| Country | Link |
|---|---|
| DE (1) | DE19926985B4 (fr) |
| WO (1) | WO2000077510A2 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006136296A1 (fr) * | 2005-06-18 | 2006-12-28 | Ge Healthcare Bio-Sciences Ab | Méthode et dispositifs pour former une multitude de puits sur un gel |
| JP2008544233A (ja) | 2005-06-18 | 2008-12-04 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | ゲル中の被分析物に試薬を加える方法及び装置 |
| GB201102385D0 (en) | 2011-02-10 | 2011-03-30 | Biocule Scotland Ltd | Two-dimensional gel electrophoresis apparatus and method |
| EP4419901A4 (fr) * | 2021-10-21 | 2025-11-19 | Zadeh Cameron O | Appareil de gel électrophorétique à multiplexage à haut débit et procédés associés |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2284484A (en) * | 1993-12-03 | 1995-06-07 | Univ London | Electrophoresis gel-matrix layer |
| DE4330307C2 (de) * | 1993-09-07 | 1995-11-02 | Georg Dr Meyer | Verwendung von Nukleinsäuregelelektrophorese zur Identifizierung von Organismenteilen und dergleichen |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2225432A (en) * | 1988-02-02 | 1990-05-30 | Inst Molekularbiologie Ak | Apparatus for electrophoretic separation of high-molecular dna in gel |
| US6071396A (en) * | 1994-12-15 | 2000-06-06 | University College London | Gel-matrix electrophoresis |
| US5785835A (en) * | 1996-04-12 | 1998-07-28 | One Lambda | Electrophoresis method and devices |
| AU753307B2 (en) * | 1997-09-19 | 2002-10-17 | Aclara Biosciences, Inc. | Capillary electroflow apparatus and method |
-
1999
- 1999-06-14 DE DE1999126985 patent/DE19926985B4/de not_active Expired - Fee Related
-
2000
- 2000-06-14 WO PCT/EP2000/005472 patent/WO2000077510A2/fr not_active Ceased
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4330307C2 (de) * | 1993-09-07 | 1995-11-02 | Georg Dr Meyer | Verwendung von Nukleinsäuregelelektrophorese zur Identifizierung von Organismenteilen und dergleichen |
| GB2284484A (en) * | 1993-12-03 | 1995-06-07 | Univ London | Electrophoresis gel-matrix layer |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000077510A3 (fr) | 2001-03-01 |
| DE19926985A1 (de) | 2001-01-11 |
| WO2000077510A2 (fr) | 2000-12-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| 8127 | New person/name/address of the applicant |
Owner name: REINHARDT, RICHARD, DR., 14195 BERLIN, DE |
|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |