[go: up one dir, main page]

CY1117970T1 - Μεθοδοι για επιλογη και ενισχυση πολυνουκλεοτιδιων - Google Patents

Μεθοδοι για επιλογη και ενισχυση πολυνουκλεοτιδιων

Info

Publication number
CY1117970T1
CY1117970T1 CY20161100871T CY161100871T CY1117970T1 CY 1117970 T1 CY1117970 T1 CY 1117970T1 CY 20161100871 T CY20161100871 T CY 20161100871T CY 161100871 T CY161100871 T CY 161100871T CY 1117970 T1 CY1117970 T1 CY 1117970T1
Authority
CY
Cyprus
Prior art keywords
molecular species
different
mixture
capture molecules
different molecular
Prior art date
Application number
CY20161100871T
Other languages
English (en)
Inventor
Andrea Sabot
Roberto Rigatti
Min-Jui Richard Shen
Original Assignee
Illumina, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina, Inc. filed Critical Illumina, Inc.
Publication of CY1117970T1 publication Critical patent/CY1117970T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

Η εφεύρεση παρέχει μεθόδους για έλεγχο της πυκνότητας διαφορετικών μοριακών ειδών επί της επιφάνειας ενός στερεού υποστρώματος. Ένα πρώτο μίγμα διαφορετικών μοριακών ειδών συνδέεται προς ένα στερεό υπόστρωμα υπό συνθήκες που συνδέουν έκαστο είδος σε επιθυμητή πυκνότητα, δημιουργώντας με τον τρόπο αυτό παραγωγοποιημένο υπόστρωμα που έχει συνδεδεμένα μόρια σύλληψης. Το παραγωγοποιημένο υπόστρωμα υφίσταται κατεργασία με ένα δεύτερο μίγμα διαφορετικών μοριακών ειδών, όπου διαφορετικά μοριακά είδη στο δεύτερο μίγμα συνδέονται ειδικώς προς τα διαφορετικά μόρια σύλληψης που συνδέονται προς το στερεό υπόστρωμα. Ένα ή περισσότερα από τα μόρια σύλληψης μπορεί αντιστρεπτά να τροποποιούνται έτσι ώστε τα μόρια σύλληψης έχουν διαφορετική δραστικότητα πριν και αφού συνδεθεί το δεύτερο μίγμα μοριακών ειδών. Σε ιδιαίτερες πραγματοποιήσεις, τα διαφορετικά μοριακά είδη είναι νουκλεϊνικά οξέα που είναι αντιστρεπτά τροποποιημένα για να έχουν διαφορετική δραστικότητα σε μία αντίδραση ενίσχυσης.
CY20161100871T 2009-08-25 2016-09-05 Μεθοδοι για επιλογη και ενισχυση πολυνουκλεοτιδιων CY1117970T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13180255.5A EP2669387B1 (en) 2009-08-25 2009-08-25 Methods for selecting and amplifying polynucleotides
PCT/US2009/054945 WO2011025477A1 (en) 2009-08-25 2009-08-25 Methods for selecting and amplifying polynucleotides
EP09848830.7A EP2470669B1 (en) 2009-08-25 2009-08-25 Methods for selecting and amplifying polynucleotides

Publications (1)

Publication Number Publication Date
CY1117970T1 true CY1117970T1 (el) 2017-05-17

Family

ID=43628274

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20161100871T CY1117970T1 (el) 2009-08-25 2016-09-05 Μεθοδοι για επιλογη και ενισχυση πολυνουκλεοτιδιων

Country Status (15)

Country Link
US (4) US8999642B2 (el)
EP (3) EP2669387B1 (el)
CN (1) CN102597256B (el)
CA (1) CA2770389C (el)
CY (1) CY1117970T1 (el)
DK (2) DK2669387T3 (el)
ES (2) ES2595055T3 (el)
HR (1) HRP20161056T1 (el)
HU (1) HUE030765T2 (el)
LT (1) LT2669387T (el)
PL (1) PL2669387T3 (el)
PT (1) PT2669387T (el)
SI (1) SI2669387T1 (el)
SM (1) SMT201600319B (el)
WO (1) WO2011025477A1 (el)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
EP3216874A1 (en) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
EP3572528A1 (en) 2010-09-24 2019-11-27 The Board of Trustees of the Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
CN103748236B (zh) 2011-04-15 2018-12-25 约翰·霍普金斯大学 安全测序系统
PL2912468T3 (pl) 2012-10-29 2019-04-30 Univ Johns Hopkins Test papanicolaou pod kątem raka jajnika i endometrium
WO2014093330A1 (en) 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
WO2015083004A1 (en) 2013-12-02 2015-06-11 Population Genetics Technologies Ltd. Method for evaluating minority variants in a sample
AU2014364180B2 (en) 2013-12-09 2021-03-04 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
US10537889B2 (en) * 2013-12-31 2020-01-21 Illumina, Inc. Addressable flow cell using patterned electrodes
BR112016016546B1 (pt) 2014-01-16 2022-09-06 Illumina, Inc Método de preparação de amplicon e métodos para determinação da presença de um gene associado a câncer e de uma variante de sequência de ácidos nucleicos verdadeira
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
US9944924B2 (en) 2014-01-16 2018-04-17 Illumina, Inc. Polynucleotide modification on solid support
SG11201610168YA (en) 2014-05-16 2017-01-27 Illumina Inc Nucleic acid synthesis techniques
US20150353989A1 (en) * 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
GB201410646D0 (en) 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
CN107208019B (zh) 2014-11-11 2021-01-01 伊鲁米纳剑桥有限公司 用于核酸单克隆簇的产生和测序的方法和阵列
CN106148482B (zh) * 2015-03-24 2019-12-03 深圳华大智造科技有限公司 一种适用于小型测序仪的测序方法
CN107922966B (zh) 2015-07-06 2022-06-28 伊卢米纳剑桥有限公司 用于核酸扩增的样品制备
ES2888626T3 (es) * 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
IL285202B2 (en) 2015-11-11 2025-03-01 Resolution Bioscience Inc High-efficiency construction of DNA libraries
JP7315326B2 (ja) 2015-11-16 2023-07-26 リヴィア バイオセンサーズ，エルエルシー 遺伝子サンプルを識別且つ区別するためのシステムと方法
US10894990B2 (en) 2016-05-17 2021-01-19 Shoreline Biome, Llc High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures
MX2019002093A (es) 2016-08-25 2019-06-20 Resolution Bioscience Inc Métodos para la detección de cambios de copia genómica en muestras de adn.
GB201615233D0 (en) * 2016-09-08 2016-10-26 Bbi Solutions Oem Ltd Solid Phase Conjugate
AU2018266675B2 (en) 2017-05-09 2024-10-10 Revere Biosensors, Llc Systems and methods for identifying and distinguishing genetic samples
MX2020001575A (es) 2017-08-07 2020-11-18 Univ Johns Hopkins Materiales y métodos para evaluar y tratar el cáncer.
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
GB2613480B (en) 2018-11-15 2023-11-22 Element Biosciences Inc Methods for generating circular nucleic acid molecules
US20220073977A1 (en) 2020-02-14 2022-03-10 The Johns Hopkins University Methods and materials for assessing nucleic acids
WO2021163621A2 (en) * 2020-02-14 2021-08-19 Genapsys, Inc. Methods and systems for nucleic acid amplification and sequencing
KR20220151101A (ko) * 2020-03-09 2022-11-14 일루미나, 인코포레이티드 폴리뉴클레오티드를 서열분석하는 방법
IL299327A (en) * 2020-09-14 2023-02-01 Illumina Cambridge Ltd Compositions and methods for amplifying polynucleotides
EP4294920A4 (en) * 2021-04-27 2025-01-22 Singular Genomics Systems, Inc. HIGH-DENSITY SEQUENCING AND MULTIPLEX PRIMING
US11427855B1 (en) 2021-06-17 2022-08-30 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
WO2023076833A1 (en) * 2021-10-26 2023-05-04 Singular Genomics Systems, Inc. Multiplexed targeted amplification of polynucleotides
CA3223090A1 (en) * 2022-03-31 2023-10-05 Xiaoyu Ma Paired-end re-synthesis using blocked p5 primers

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4292503A (en) 1979-05-14 1981-09-29 Emerson Electric Co. Split-band electric heater
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0224126A3 (en) 1985-11-25 1989-02-01 The University of Calgary Covalently linked complementary oligodeoxynucleotides as universal nucleic acid sequencing primer linkers
WO1989001050A1 (en) 1987-07-31 1989-02-09 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US6107023A (en) 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
GB8818020D0 (en) 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
CA2004326A1 (en) 1988-12-23 1990-06-23 Nanibushan Dattagupta Assay of sequences using amplified genes
US5629158A (en) 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
JP3096722B2 (ja) 1990-01-17 2000-10-10 日本ケミカルリサーチ株式会社 ポリメラーゼ・チェーン・リアクション用プライマーの作製法およびそれを用いるｄｎａの増幅法
US5118604A (en) 1990-06-15 1992-06-02 Yale University Restriction-fragment DNA probes and probe clusters
WO1993004199A2 (en) 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
AU672760B2 (en) 1991-09-24 1996-10-17 Keygene N.V. Selective restriction fragment amplification: a general method for DNA fingerprinting
EP0675966B1 (en) 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5849544A (en) 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
RU2048522C1 (ru) 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
JPH07203998A (ja) 1994-01-26 1995-08-08 Hamamatsu Photonics Kk 核酸塩基配列決定方法
DE69527355T2 (de) 1994-05-28 2003-03-06 Tepnel Medical Ltd., Manchester Herstellung von nukleinsaeurekopien
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6090592A (en) 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
US6060288A (en) 1994-08-03 2000-05-09 Mosaic Technologies Method for performing amplification of nucleic acid on supports
JPH11503611A (ja) 1995-04-11 1999-03-30 トラスティーズ・オブ・ボストン・ユニバーシティ 生体高分子の固相配列決定法
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
DE19515552A1 (de) 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
SE9504099D0 (sv) 1995-11-16 1995-11-16 Pharmacia Biotech Ab A method of sequencing
US5935788A (en) 1996-06-25 1999-08-10 Lifespan Biosciences, Inc. Subtractive hybridization techniques for identifying differentially expressed and commonly expressed nucleic acid
US5837466A (en) 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
US20020061532A1 (en) 1997-02-14 2002-05-23 Mosaic Technologies, Inc. Method and apparatus for performing amplification of nucleic acids on supports
DE69837913T2 (de) 1997-04-01 2008-02-07 Solexa Ltd., Saffron Walden Verfahren zur vervielfältigung von nukleinsäure
EP0975802B1 (en) 1997-04-01 2004-06-23 Manteia S.A. Method of nucleic acid sequencing
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US20030022207A1 (en) 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
ATE316152T1 (de) 1998-10-27 2006-02-15 Affymetrix Inc Komplexitätsmanagement und analyse genomischer dna
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20030108867A1 (en) 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
DE10051564A1 (de) 2000-10-18 2002-08-01 Axaron Bioscience Ag Neue Verfahren zur parallelen Sequenzierung eines Nukleinsäuregemisches an einer Oberfläche
DE60025850T2 (de) 1999-12-29 2006-08-03 Keygene N.V. Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
WO2001079553A1 (en) 2000-04-14 2001-10-25 Lynx Therapeutics, Inc. Method and compositions for ordering restriction fragments
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
WO2002084285A2 (en) * 2001-04-18 2002-10-24 Krull Ulrich J Gradient resolved hybridisation platform
EP1256632A3 (en) 2001-05-07 2004-01-02 Smithkline Beecham Corporation High throughput polymorphism screening
US20030219770A1 (en) 2001-11-08 2003-11-27 Eshleman James R. Methods and systems of nucleic acid sequencing
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
CA2478722A1 (en) 2002-03-05 2003-09-12 Solexa Limited Methods for detecting genome-wide sequence variations associated with a phenotype
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
AU2003242605A1 (en) * 2002-06-03 2003-12-19 Pamgene B.V. Novel high density arrays and methods for analyte analysis
US9388459B2 (en) 2002-06-17 2016-07-12 Affymetrix, Inc. Methods for genotyping
DK3002289T3 (en) 2002-08-23 2018-04-23 Illumina Cambridge Ltd MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCE
US20040126765A1 (en) 2002-12-27 2004-07-01 Adams Craig W. Method and compositions for sequencing nucleic acid molecules
AU2004209426B2 (en) 2003-01-29 2008-04-17 454 Life Sciences Corporation Method for preparing single-stranded DNA libraries
JP2006517798A (ja) 2003-02-12 2006-08-03 イェニソン スベンスカ アクティエボラーグ 核酸配列用の方法及び手段
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
EP1682680B2 (en) 2003-10-31 2018-03-21 AB Advanced Genetic Analysis Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
GB2412170A (en) 2004-03-16 2005-09-21 Plant Bioscience Ltd A method for enriching for target nucleic acids in a sample
GB2413796B (en) 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
US20070003924A1 (en) 2004-06-18 2007-01-04 The Ohio State University Research Foundation Serial analysis of ribosomal and other microbial sequence tags
US20060078899A1 (en) 2004-10-12 2006-04-13 Scheffer Alicia F Methods and compositions for reducing label variation in array-based comparative genome hybridization assays
WO2006110855A2 (en) 2005-04-12 2006-10-19 454 Life Sciences Corporation Methods for determining sequence variants using ultra-deep sequencing
CA2615323A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US8428882B2 (en) 2005-06-14 2013-04-23 Agency For Science, Technology And Research Method of processing and/or genome mapping of diTag sequences
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
DE602007009634D1 (de) 2006-01-04 2010-11-18 Si Lok Verfahren zur zuordnung von nukleinsäuren und zur identifikation fein strukturierter variationen in nukleinsäuren sowie hilfsmittel dafür
US8192930B2 (en) 2006-02-08 2012-06-05 Illumina Cambridge Limited Method for sequencing a polynucleotide template
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
WO2007111937A1 (en) 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
WO2007135368A2 (en) 2006-05-18 2007-11-29 Solexa Limited Dye compounds and the use of their labelled conjugates
WO2008002502A2 (en) 2006-06-23 2008-01-03 Illumina, Inc. Devices and systems for creation of dna cluster arrays
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
GB0712882D0 (en) 2007-07-03 2007-08-15 Leicester University Of Nucleic acid amplification
WO2009032167A1 (en) 2007-08-29 2009-03-12 Illumina Cambridge Method for sequencing a polynucleotide template
JP2009178159A (ja) * 2007-11-05 2009-08-13 Hitachi Plant Technologies Ltd 核酸配列の検出方法及び核酸配列検出基板
US8728764B2 (en) 2008-10-02 2014-05-20 Illumina Cambridge Limited Nucleic acid sample enrichment for sequencing applications
EP3572528A1 (en) 2010-09-24 2019-11-27 The Board of Trustees of the Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support

Also Published As

Publication number Publication date
SI2669387T1 (sl) 2017-01-31
EP2470669A1 (en) 2012-07-04
US20200239875A1 (en) 2020-07-30
US20170283794A1 (en) 2017-10-05
ES2595055T3 (es) 2016-12-27
EP2669387B1 (en) 2016-07-20
CA2770389A1 (en) 2011-03-03
DK2669387T3 (en) 2016-09-19
DK3133169T3 (da) 2019-12-16
CA2770389C (en) 2015-12-29
SMT201600319B (it) 2016-11-10
EP2470669A4 (en) 2013-01-30
US20120122737A1 (en) 2012-05-17
EP2470669B1 (en) 2014-06-18
WO2011025477A1 (en) 2011-03-03
CN102597256A (zh) 2012-07-18
US20150148263A1 (en) 2015-05-28
US10597653B2 (en) 2020-03-24
ES2763386T3 (es) 2020-05-28
US11142759B2 (en) 2021-10-12
PL2669387T3 (pl) 2016-12-30
US8999642B2 (en) 2015-04-07
HUE030765T2 (en) 2017-05-29
LT2669387T (lt) 2016-10-25
CN102597256B (zh) 2014-12-03
EP3133169A1 (en) 2017-02-22
HRP20161056T1 (hr) 2016-12-16
EP3133169B1 (en) 2019-10-16
EP2669387A1 (en) 2013-12-04
US9624489B2 (en) 2017-04-18
PT2669387T (pt) 2016-09-20

Similar Documents

Publication Publication Date Title
CY1117970T1 (el) Μεθοδοι για επιλογη και ενισχυση πολυνουκλεοτιδιων
CY1112739T1 (el) Αντισωματα και ανοσοσυζευγματα αντι-cd79β και μεθοδοι χρησης
CY1123441T1 (el) Μορια με ειδικοτητα στα cd45 και cd79
CY1124232T1 (el) Συνδετικα μορια συνδεσης pd-l1 και lag-3
MX2021015230A (es) Nanoparticula con un solo sitio para la union de polinucleotido de plantilla.
CY1121244T1 (el) Συνδυασμος αναστολεα τελομερασης και γεμσιταβινής για τη θεραπεια του καρκινου
CY1124747T1 (el) Ανθρωπινα αντισωματα σε pd-1
WO2020010250A3 (en) Anti-tcr antibody molecules and uses thereof
CY1117859T1 (el) Αντισωματα και ανοσοσυζευγματα anti-cd79b και μεθοδοι χρησης
CY1122005T1 (el) Ανθρωπινα αντισωματα κατα toy pd-l1
CY1121101T1 (el) Μεθοδοι και στερεα υποστρωματα για παρασκευη δειγματος χρησιμοποιωντας τρανσποσωματα
CY1126115T1 (el) Συνδυασμος anti-lag-3 αντισωματων και anti-pd-1 αντισωματων για θεραπεια ογκων
CY1120770T1 (el) Ζωα πλην του ανθρωπου με τροποποιημενες αλληλουχιες βαριας αλυσιδας της ανοσοσφαιρινης
CY1119994T1 (el) Συνθεσεις και μεθοδοι για αυξηση της μυϊκης μαζας και της μυϊκης δυναμης ανταγωνιζοντας ειδικα ton gdf8 και/ή την ακτιβινη α
CY1121294T1 (el) Αντι-ilt7 αντισωμα
EA200970737A1 (ru) Оксабициклогептаны и оксабициклогептены, их получение и применение
CY1119696T1 (el) Cc-1065 αναλογα και τα συζευγματα αυτων
EA201890222A1 (ru) Полиспецифические антигенсвязывающие молекулы и их применения
CY1118244T1 (el) Αντισωματα κατα της il-25
CY1116970T1 (el) Fkbp-l και χρησεις αυτων ως αναστολεις αγγειογενεσης
TR201808535T4 (tr) İki veya daha fazla sayıda antijen molekülüne tekrar tekrar bağlanabilen antijen bağlanma molekülü.
MX343063B (es) Metodos y composiciones para producir hidrocarburos.
ATE527385T1 (de) Mit tumoren assoziierte genetische variationen
CY1110895T1 (el) Περιοχες συνθετικης ανοσοσφαιρινης με ιδιοτητες δεσμευσης τροποποιημενες σε περιοχες του μοριου διαφορετικες απο τις περιοχες προσδιορισμου συμπληρωματικοτητας
NZ598791A (en) Anti-gcc antibody molecules and related compositions and methods