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CY1112664T1 - Μεθοδος κλωνοποιησης συγγενων αντισωματων - Google Patents

Μεθοδος κλωνοποιησης συγγενων αντισωματων

Info

Publication number
CY1112664T1
CY1112664T1 CY20111101105T CY111101105T CY1112664T1 CY 1112664 T1 CY1112664 T1 CY 1112664T1 CY 20111101105 T CY20111101105 T CY 20111101105T CY 111101105 T CY111101105 T CY 111101105T CY 1112664 T1 CY1112664 T1 CY 1112664T1
Authority
CY
Cyprus
Prior art keywords
linking
procedure
generation
methods
relates
Prior art date
Application number
CY20111101105T
Other languages
English (en)
Inventor
Per-Johan Meijer
Lars Soegaard Nielsen
Jesper Kastrup
Original Assignee
Symphogen A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38561767&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1112664(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Symphogen A/S filed Critical Symphogen A/S
Publication of CY1112664T1 publication Critical patent/CY1112664T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1282—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Η εφεύρεση σχετίζεται με μία διαδικασία σύνδεσης συγγενών ζευγών αλυσίδων κωδικοποίησης VH και VL από ένα πληθυσμό κυττάρων που είναι εμπλουτισμένος ιδιαίτερα σε παράγοντες σήμανσης επιφανειακού αντιγόνου. Η διαδικασία σύνδεσης περιλαμβάνει μία διαδικασία πολλαπλής μοριακής ενίσχυσης ικανή να συνδέσει αλυσίδες νουκλεοτιδίων που τυγχάνουν ενδιαφέροντος σε σχέση με την ενίσχυση, ειδικότερα με μια πολλαπλή αλυσιδωτή αντίδραση πολυμεράσης (polymerase) chain reaction (πολλαπλή PCR). Η μέθοδος είναι ιδιαίτερα πλεονεκτική για την δημιουργία βιβλιοθηκών συγγενών ζευγών καθώς και συνδυαστικών βιβλιοθηκών αλυσίδων κωδικοποίησης μεταβλητής περιοχής από ανοσογλοβουλίνες. Η εφεύρεση σχετίζεται επίσης με την δημιουργία χιμαιρικών ανθρώπινων / μη ανθρώπινων αντισωμάτων και έκφραση των βιβλιοθηκών που δημιουργούνται με τέτοιες μεθόδους.
CY20111101105T 2007-03-01 2011-11-16 Μεθοδος κλωνοποιησης συγγενων αντισωματων CY1112664T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200700316 2007-03-01
US90477207P 2007-03-05 2007-03-05
EP08706942A EP2121920B1 (en) 2007-03-01 2008-02-27 Method for cloning cognate antibodies

Publications (1)

Publication Number Publication Date
CY1112664T1 true CY1112664T1 (el) 2016-02-10

Family

ID=38561767

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20111101105T CY1112664T1 (el) 2007-03-01 2011-11-16 Μεθοδος κλωνοποιησης συγγενων αντισωματων

Country Status (23)

Country Link
US (2) US8283294B2 (el)
EP (1) EP2121920B1 (el)
JP (1) JP5669397B2 (el)
KR (1) KR20090118993A (el)
CN (1) CN101622346A (el)
AT (1) ATE521704T1 (el)
AU (1) AU2008221119B2 (el)
BR (1) BRPI0808171A2 (el)
CA (1) CA2677771A1 (el)
CY (1) CY1112664T1 (el)
DK (1) DK2121920T3 (el)
ES (1) ES2369946T3 (el)
HR (1) HRP20110686T1 (el)
IL (1) IL199773A0 (el)
MX (1) MX2009008908A (el)
NZ (1) NZ578851A (el)
PL (1) PL2121920T3 (el)
PT (1) PT2121920E (el)
RU (1) RU2459868C2 (el)
SI (1) SI2121920T1 (el)
TW (1) TW200911986A (el)
WO (1) WO2008104184A2 (el)
ZA (1) ZA200905831B (el)

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DK2121920T3 (da) 2011-11-21
RU2459868C2 (ru) 2012-08-27
IL199773A0 (en) 2010-04-15
EP2121920B1 (en) 2011-08-24
SI2121920T1 (sl) 2011-12-30
US20080227660A1 (en) 2008-09-18
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PT2121920E (pt) 2011-10-03
PL2121920T3 (pl) 2012-01-31
AU2008221119B2 (en) 2013-11-14
US20130023447A1 (en) 2013-01-24
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RU2009136342A (ru) 2011-04-10
ZA200905831B (en) 2011-02-23
WO2008104184A3 (en) 2008-10-16
BRPI0808171A2 (pt) 2014-08-05
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