[go: up one dir, main page]

MX2009008908A - Metodo para clonacion de anticuerpos analogos. - Google Patents

Metodo para clonacion de anticuerpos analogos.

Info

Publication number
MX2009008908A
MX2009008908A MX2009008908A MX2009008908A MX2009008908A MX 2009008908 A MX2009008908 A MX 2009008908A MX 2009008908 A MX2009008908 A MX 2009008908A MX 2009008908 A MX2009008908 A MX 2009008908A MX 2009008908 A MX2009008908 A MX 2009008908A
Authority
MX
Mexico
Prior art keywords
linking
procedure
generation
methods
relates
Prior art date
Application number
MX2009008908A
Other languages
English (en)
Inventor
Per-Johan Meijer
Lars Soegaard Nielsen
Jesper Kastrup
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38561767&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2009008908(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Symphogen As filed Critical Symphogen As
Publication of MX2009008908A publication Critical patent/MX2009008908A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1282—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La invención se relaciona con un procedimiento para enlazar pares análogos de secuencias que codifican para Vx y VL a partir de una población de células enriquecidas en marcadores de antígeno de superficie particular. El procedimiento de enlace involucra un procedimiento de amplificación molecular múltiplex capaz de enlazar secuencias nucleotídicas de interés en relación con la amplificación, en particular reacción en cadena de polimerasa (PCR múltiplex) El método es particularmente ventajoso para la generación de bibliotecas de pares análogos así como bibliotecas combinatorias de secuencias que codifican para la región variable de inmunoglobulinas. La invención también se relaciona con metodos para generación de anticuerpos quiméricos humanos/no humanos y bibliotecas de expresión generadas por los métodos.
MX2009008908A 2007-03-01 2008-02-27 Metodo para clonacion de anticuerpos analogos. MX2009008908A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200700316 2007-03-01
US90477207P 2007-03-05 2007-03-05
PCT/DK2008/050048 WO2008104184A2 (en) 2007-03-01 2008-02-27 Method for cloning cognate antibodies

Publications (1)

Publication Number Publication Date
MX2009008908A true MX2009008908A (es) 2009-08-28

Family

ID=38561767

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009008908A MX2009008908A (es) 2007-03-01 2008-02-27 Metodo para clonacion de anticuerpos analogos.

Country Status (23)

Country Link
US (2) US8283294B2 (es)
EP (1) EP2121920B1 (es)
JP (1) JP5669397B2 (es)
KR (1) KR20090118993A (es)
CN (1) CN101622346A (es)
AT (1) ATE521704T1 (es)
AU (1) AU2008221119B2 (es)
BR (1) BRPI0808171A2 (es)
CA (1) CA2677771A1 (es)
CY (1) CY1112664T1 (es)
DK (1) DK2121920T3 (es)
ES (1) ES2369946T3 (es)
HR (1) HRP20110686T1 (es)
IL (1) IL199773A0 (es)
MX (1) MX2009008908A (es)
NZ (1) NZ578851A (es)
PL (1) PL2121920T3 (es)
PT (1) PT2121920E (es)
RU (1) RU2459868C2 (es)
SI (1) SI2121920T1 (es)
TW (1) TW200911986A (es)
WO (1) WO2008104184A2 (es)
ZA (1) ZA200905831B (es)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008221118B2 (en) 2007-03-01 2013-10-03 Les Laboratoires Servier Recombinant anti-epidermal growth factor receptor antibody compositions
JP2008289483A (ja) * 2007-05-25 2008-12-04 Symphogen As 真核生物系において発現可能な形質転換体のスクリーニング
DE102007037215A1 (de) * 2007-08-07 2009-02-12 Robert Bosch Gmbh Stellorgan
WO2010000864A1 (en) * 2008-07-04 2010-01-07 Novo Nordisk A/S Method for producing monoclonal antibodies
JP5719298B2 (ja) 2008-08-29 2015-05-13 シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ 組換え抗上皮増殖因子受容体抗体組成物
KR20110058861A (ko) * 2008-08-29 2011-06-01 심포젠 에이/에스 조류 유래 항체의 클로닝 방법
GB2467704B (en) * 2008-11-07 2011-08-24 Mlc Dx Inc A method for determining a profile of recombined DNA sequences in T-cells and/or B-cells
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US8691510B2 (en) * 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
PL2387627T3 (pl) 2009-01-15 2016-10-31 Profilowanie odporności adaptacyjnej i wytwarzanie przeciwciał monoklonalnych
US9079942B2 (en) * 2009-02-09 2015-07-14 Epitomics, Inc. CDR-anchored amplification method
JP5684734B2 (ja) * 2009-02-20 2015-03-18 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 免疫グロブリンをコードする核酸を得る方法
US20100317539A1 (en) * 2009-06-12 2010-12-16 Guo-Liang Yu Library of Engineered-Antibody Producing Cells
KR20120044941A (ko) 2009-06-25 2012-05-08 프레드 헛친슨 켄서 리서치 센터 적응 면역의 측정방법
US8293483B2 (en) 2009-09-11 2012-10-23 Epitomics, Inc. Method for identifying lineage-related antibodies
US9043160B1 (en) 2009-11-09 2015-05-26 Sequenta, Inc. Method of determining clonotypes and clonotype profiles
DE102010037622B4 (de) 2010-09-17 2012-07-12 Lophius Biosciences Gmbh Verfahren zum Nachweis, Differenzieren und Quantifizieren von T-Zellpopulationen mittels der Reverse Transkription quantitativen Real-Time PCR (RT-qPCR) Technologie
GB2498163B (en) * 2010-10-08 2015-07-01 Harvard College High-throughput immune sequencing
US9155802B2 (en) 2010-11-01 2015-10-13 Symphogen A/S Pan-HER antibody composition
EP2647704B1 (en) * 2010-12-01 2017-11-01 Mitsubishi Tanabe Pharma Corporation Polynucleotide construct capable of presenting fab in acellular translation system, and method for manufacturing and screening fab using same
WO2012104851A1 (en) * 2011-01-31 2012-08-09 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
ES2925799T3 (es) 2011-04-28 2022-10-19 Univ Leland Stanford Junior Identificación de polinucleótidos asociados a una muestra
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
EP2758428A1 (en) * 2011-09-21 2014-07-30 F.Hoffmann-La Roche Ag METHOD FOR OBTAINING FAB FRAGMENTS FROM SINGLE ANTIBODY PRODUCING CELLS BY MULTIPLEXED PCR IN COMBINATION WITH TaqMan PROBES
CA2853088C (en) 2011-10-21 2018-03-13 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
ES2867924T3 (es) 2011-12-09 2021-10-21 Adaptive Biotechnologies Corp Diagnóstico de tumores malignos linfoides y detección de enfermedad residual mínima
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
EP2626433B1 (en) * 2012-02-09 2017-04-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for linking nucleic acids in a microsome from endoplasmatic reticuli
ES2662128T3 (es) 2012-03-05 2018-04-05 Adaptive Biotechnologies Corporation Determinación de cadenas de receptor inmunitario emparejadas a partir de la frecuencia de subunidades coincidentes
US9527913B2 (en) 2012-05-02 2016-12-27 Symphogen A/S Humanized pan-HER antibody compositions
JP5756247B1 (ja) 2012-05-08 2015-07-29 アダプティブ バイオテクノロジーズ コーポレイション マルチプレックスｐｃｒ反応における増幅バイアスを測定し校正する組成物及び方法
DK2861760T3 (da) 2012-06-15 2020-03-09 Univ Texas Sekventering med høj kapacitet af flere udskrifter af en enkelt celle
ES2660027T3 (es) 2012-10-01 2018-03-20 Adaptive Biotechnologies Corporation Evaluación de la inmunocompetencia por la diversidad de los receptores de inmunidad adaptativa y caracterización de la clonalidad
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
CN103074349B (zh) * 2012-11-07 2014-10-15 杭州鸿运华宁生物医药工程有限公司 巢式一步法rt-pcr从单个b细胞扩增鼠源抗体重链和轻链可变区基因全序列的方法
CA2903025C (en) * 2013-03-11 2025-10-07 Regeneron Pharmaceuticals, Inc. TRANSGENIC MICE EXPRESSING CHIMERIC MAJOR HISTOCOMPATIBILITY COMPLEX (MHC) CLASS II MOLECULES
AU2014232225B2 (en) * 2013-03-15 2020-03-19 H. Lundbeck A/S. Protocol for identifying and isolating antigen-specific B cells and producing antibodies to desired antigens
BR112015029754A2 (pt) 2013-05-31 2017-09-26 Genentech Inc anticorpos anti-teicoico da parede e conjugados
BR112015029838A2 (pt) 2013-05-31 2017-09-26 Genentech Inc anticorpos, composições, ácido nucleico isolado, vetor, célula hospedeira isolada, método de produção de anticorpos, composto, conjugado de anticorpo e antibiótico, processo de elaboração do composto, intermediário entre ligante e antibiótico, método de tratamento de infecções bacterianas e método de matar staphylococcus aureus
US9803002B2 (en) 2013-05-31 2017-10-31 Genentench, Inc. Anti-wall teichoic antibodies and conjugates
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
EP3114240B1 (en) 2014-03-05 2019-07-24 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
AU2015339191A1 (en) 2014-10-29 2017-05-18 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
AU2015353581A1 (en) 2014-11-25 2017-06-15 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
CN107206102A (zh) 2014-12-03 2017-09-26 豪夫迈·罗氏有限公司 抗金黄色葡萄球菌抗体利福霉素缀合物及其用途
WO2016138122A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
US10513733B2 (en) 2015-03-23 2019-12-24 Board Of Regents, The University Of Texas System High throughout sequencing of paired VH and VL transcripts from B cells secreting antigen-specific antibodies
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
US9422547B1 (en) 2015-06-09 2016-08-23 Gigagen, Inc. Recombinant fusion proteins and libraries from immune cell repertoires
EP4345107A3 (en) * 2015-06-09 2024-06-12 Gigagen, Inc. Recombinant fusion proteins and libraries from immune cell repertoires
MY190324A (en) 2015-10-02 2022-04-14 Symphogen As Anti-pd-1 antibodies and compositions
BR112018014355A2 (pt) 2016-03-04 2018-12-18 Genentech, Inc. processos para a preparação de f-benzoxazinorifamicina i e para a preparação de 2-amino-5-fluorobenzeno-1,3-diol iii
WO2017198731A1 (en) 2016-05-18 2017-11-23 Genmab B.V. Antibodies and methods of use thereof in treatment of infectious disease
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
WO2018091661A1 (en) 2016-11-18 2018-05-24 Symphogen A/S Anti-pd-1 antibodies and compositions
WO2018119402A1 (en) * 2016-12-23 2018-06-28 Visterra, Inc. Binding polypeptides and methods of making the same
US11866785B2 (en) 2017-10-27 2024-01-09 Board Of Regents, The University Of Texas System Tumor specific antibodies and T-cell receptors and methods of identifying the same
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
AU2019403404B2 (en) 2018-12-21 2025-05-29 Regeneron Pharmaceuticals, Inc. Rifamycin analogs and antibody-drug conjugates thereof
CN109972209B (zh) * 2019-01-18 2022-05-03 南开大学 基于乳液pcr的一步法将单链抗体库重排为全长抗体库的方法
WO2020191365A1 (en) 2019-03-21 2020-09-24 Gigamune, Inc. Engineered cells expressing anti-viral t cell receptors and methods of use thereof
WO2022271782A1 (en) * 2021-06-22 2022-12-29 University Of Virginia Patent Foundation Compositions and methods for detecting and regulating fibronectin-integrin interactions and signaling
CN115109794A (zh) * 2022-06-30 2022-09-27 武汉生之源生物科技股份有限公司 一种噬菌粒载体及其制备方法和应用

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5576195A (en) 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
US5618920A (en) 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
US5906936A (en) 1988-05-04 1999-05-25 Yeda Research And Development Co. Ltd. Endowing lymphocytes with antibody specificity
WO1992015678A1 (en) 1991-03-01 1992-09-17 Stratagene Pcr generated dicistronic dna molecules for producing antibodies
US6277969B1 (en) * 1991-03-18 2001-08-21 New York University Anti-TNF antibodies and peptides of human tumor necrosis factor
US6284471B1 (en) * 1991-03-18 2001-09-04 New York University Medical Center Anti-TNFa antibodies and assays employing anti-TNFa antibodies
WO1993003151A1 (en) 1991-08-10 1993-02-18 Medical Research Council Treatment of cell populations
ES2153379T3 (es) 1992-03-31 2001-03-01 Abbott Lab Procedimiento de reaccion de ligasa en cadena multiplex.
GB9220808D0 (en) 1992-10-02 1992-11-18 Medical Res Council Antibody genes
US6096878A (en) 1993-05-10 2000-08-01 Japan Tobacco Inc. Human immunoglobulin VH gene segments and DNA fragments containing the same
DK0744958T3 (da) 1994-01-31 2003-10-20 Univ Boston Polyklonale antistofbiblioteker
FR2724393A1 (fr) * 1994-09-12 1996-03-15 Inst Nat Sante Rech Med Obtention d'un anticorps monoclonal recombinant humanise a partir d'un anticorps monoclonal murin, sa production en cellules d'insecte, et ses utilisations
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
US6258529B1 (en) 1994-12-01 2001-07-10 Oravax, Inc. PCR amplification of rearranged genomic variable regions of immunoglobulin genes
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
WO1998047343A2 (en) 1997-04-04 1998-10-29 Biosite Diagnostics, Inc. Antibodies or binding protein libraries displayed on phage, cells, or other replicatable genetic packages
US6610293B1 (en) * 1997-06-16 2003-08-26 The Henry M. Jackson Foundation For The Advancement Of Military Medicine Opsonic and protective monoclonal and chimeric antibodies specific for lipoteichoic acid of gram positive bacteria
WO1999016904A1 (en) 1997-09-29 1999-04-08 City Of Hope Efficient linking of nucleic acid segments
ATE531812T1 (de) * 1997-12-05 2011-11-15 Scripps Research Inst Humanisierung von nager-antikörpern
US6194551B1 (en) 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
CA2382165A1 (en) 1999-12-08 2001-06-14 Genset S.A. Full-length human cdnas encoding potentially secreted proteins
JP2003534290A (ja) 2000-05-26 2003-11-18 シムフォゲン アクティーゼルスカブ アレルギー治療用の組換えまたは精製ポリクロナール抗体
IL136459A0 (en) 2000-05-30 2001-06-14 Galim Galil Immunology Ltd Antibody library
US6849259B2 (en) 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
US6994963B1 (en) 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6867021B2 (en) 2001-02-20 2005-03-15 Board Of Trustees Of Michigan State University Multiplex RT-PCR/PCR for simultaneous detection of bovine coronavirus, bovine rotavirus, Cryptosporidium parvum, and Escherichia coli
DE60305919T2 (de) * 2002-06-28 2007-01-18 Domantis Limited, Cambridge Dual-specifische liganden mit erhöhter halbwertszeit
US20040067532A1 (en) * 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
DK1583830T3 (da) 2003-01-07 2007-01-15 Symphogen As Fremgangsmåde til fremstilling af rekombinante polyklonale proteiner
US20060275766A1 (en) 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
US20050090453A1 (en) * 2003-01-15 2005-04-28 Solbec Pharmaceuticals Co., Ltd. Methods of modulating IL-6
US20060228350A1 (en) * 2003-08-18 2006-10-12 Medimmune, Inc. Framework-shuffling of antibodies
RU2392324C2 (ru) * 2003-09-18 2010-06-20 Симфоген А/С Способ связывания интересующих последовательностей
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
DK1692276T3 (da) 2003-11-19 2010-11-01 Us Gov Health & Human Serv Fremgangsmåde til induktion af udvikling og terminal differentiering af hukommelses-B-celler
RU2426795C2 (ru) 2004-07-20 2011-08-20 Симфоген А/С Способ структурной характеристики рекомбинантного поликлонального белка или поликлональной клеточной линии
BRPI0513706A (pt) 2004-07-20 2008-05-13 Symphogen As anticorpo policlonal recombinante anti-rhesus d e métodos de produção
US20060094645A1 (en) * 2004-10-06 2006-05-04 Oliver Lawless Method for defining and treating chemically-induced immune disorders using tumor necrosis factor (TFNalpha), interleukin-1 (lL-1), and interleulin-6R(lL-6R) antagonists
US8945857B2 (en) * 2005-07-01 2015-02-03 John Schrader Methods of isolating cells and generating monoclonal antibodies
EP2314619A1 (en) 2005-12-05 2011-04-27 Symphogen A/S Anti-orthopoxvirus recombinant polyclonal antibody
CA2638833A1 (en) 2006-03-06 2007-09-13 Symphogen A/S Recombinant polyclonal antibody for treatment of respiratory syncytial virus infections
AU2008221118B2 (en) 2007-03-01 2013-10-03 Les Laboratoires Servier Recombinant anti-epidermal growth factor receptor antibody compositions
AU2008224259A1 (en) 2007-03-06 2008-09-12 Symphogen A/S Recombinant antibodies for treatment of respiratory syncytial virus infections
PT2152872E (pt) 2007-05-25 2010-11-17 Symphogen As Método para o fabrico de uma proteína policlonal recombinante
CN101821289A (zh) 2007-09-07 2010-09-01 西福根有限公司 重组制造抗rsv抗体的方法
KR20110058861A (ko) 2008-08-29 2011-06-01 심포젠 에이/에스 조류 유래 항체의 클로닝 방법

Also Published As

Publication number Publication date
CA2677771A1 (en) 2008-09-04
HK1133905A1 (en) 2010-04-09
NZ578851A (en) 2011-09-30
DK2121920T3 (da) 2011-11-21
RU2459868C2 (ru) 2012-08-27
IL199773A0 (en) 2010-04-15
EP2121920B1 (en) 2011-08-24
SI2121920T1 (sl) 2011-12-30
US20080227660A1 (en) 2008-09-18
HRP20110686T1 (hr) 2011-10-31
JP5669397B2 (ja) 2015-02-12
PT2121920E (pt) 2011-10-03
PL2121920T3 (pl) 2012-01-31
AU2008221119B2 (en) 2013-11-14
US20130023447A1 (en) 2013-01-24
WO2008104184A2 (en) 2008-09-04
US8283294B2 (en) 2012-10-09
CY1112664T1 (el) 2016-02-10
CN101622346A (zh) 2010-01-06
JP2010535150A (ja) 2010-11-18
ES2369946T3 (es) 2011-12-09
KR20090118993A (ko) 2009-11-18
RU2009136342A (ru) 2011-04-10
ZA200905831B (en) 2011-02-23
WO2008104184A3 (en) 2008-10-16
BRPI0808171A2 (pt) 2014-08-05
TW200911986A (en) 2009-03-16
AU2008221119A1 (en) 2008-09-04
EP2121920A2 (en) 2009-11-25
ATE521704T1 (de) 2011-09-15

Similar Documents

Publication Publication Date Title
IL199773A0 (en) Method for cloning cognate antibodies
WO2008069906A3 (en) Digital expression of gene analysis
EP4105644A3 (en) Systems and devices for sequence by synthesis analysis
MX338063B (es) Molecula de union especifica al rsv.
WO2006074162A3 (en) Primer generation rolling circle amplification
WO2007136736A3 (en) Methods for nucleic acid sorting and synthesis
IN2012DN00403A (es)
CL2011000442A1 (es) Secuencia de acido nucleico de locus de floracion de remolacha azucarera; polipeptido codificados por dichas secuencias, vector de expresion que comprende dichos polinucleotidos, y procedimiento para prodiucir semillas hibridas con resistencia al espigado.
WO2008052101A3 (en) Multiplex automated genome engineering
WO2006081222A3 (en) Isothermal dna amplification
WO2008078180A3 (en) Materials and methods for the generation of transcripts comprising modified nucleotides
WO2015081229A3 (en) Selective amplification of nucleic acid sequences
MX2010002463A (es) Plantas con rasgos relacionados con un rendimiento aumentado y un metodo para producirlas.
WO2008042067A3 (en) Compositions and methods for nucleotide sequencing
NZ591543A (en) Improved antibody libraries
WO2008061129A3 (en) Methods and compositions related to continuous flow thermal gradient pcr
WO2008058282A3 (en) Methods and compositions for large-scale analysis of nucleic acids using dna deletions
WO2009108918A3 (en) Methods and systems for social networking based on nucleic acid sequences
AU2009209251A8 (en) Stabilized Angiopoietin-2 antibodies and uses thereof
MX346024B (es) Optimización de solubilidad de inmuno-aglutinantes.
WO2005118806A3 (en) METHODS AND COMPOSITIONS INVOLVING MicroRNA
WO2008115632A3 (en) Method for recombining dna sequences and compositions related thereto
MX2009007246A (es) Plantas con rasgos relacionados con el rendimiento mejorado y un metodo para producirlas.
AU2009355009A8 (en) THD primer target detection
WO2010075414A3 (en) Method for detection of xmrv

Legal Events

Date Code Title Description
HH Correction or change in general
FG Grant or registration