CN1985791B - Preparation method of skin care silk fibroin gel cream - Google Patents

Abstract

The invention discloses a preparation method of silk gel cream for skin care. The preparation step comprises: adding water-soluble sericin or water-soluble sericin and silk peptide as a gel accelerator to silk fibroin aqueous solution , after uniform mixing, the solution is left to stand at 0-90°C until the solution gels into a translucent silk fibroin gel, and the water content of the silk fibroin gel is controlled within the range of 98%-70%. The prepared silk fibroin gel is homogeneously emulsified into a cream. According to needs, other cosmetic base components and auxiliary components can be added in an appropriate amount in the preparation step. Because the silk fibroin gel in the present invention not only has the function of nutrition additive, but also has the function of moisturizing and matrix, so the present invention can be used to produce high-quality natural beauty and skin care products.

Description

Preparation method of skin care silk fibroin gel cream

technical field

The invention relates to a preparation method of a silk fibroin gel cream used for beauty, skin care and skin repair.

Background technique

With the improvement of living standards, people pay more and more attention to beauty and skin care, and the quality requirements of beauty and skin care products are also getting higher and higher. Cosmetic and skin care products containing natural active ingredients extracted from organisms such as collagen, silk fibroin, seaweed extract, and ginseng extract not only protect the skin, but also have the functions of conditioning the skin and promoting skin metabolism, and are very popular. Silk has a good affinity with human skin, is breathable and absorbs water, and has been known as the queen of fibers since ancient times. Silk fibroin is a natural protein extracted from silk, consisting of 18 kinds of amino acids. Studies have shown that silk fibroin has growth-promoting biological activity on most mammalian cells such as fibroblasts, epidermal cells, epithelial cells, and chondrocytes, so silk fibroin is used in various cosmetic skin care products and artificial skin and other medical materials .

Usually, silk fibroin only acts as a nutritional additive in beauty and skin care products, but does not have a moisturizing function, nor does it have the function of a matrix, which means that the efficacy of silk fibroin has not been fully exerted. The reason is that silk fibroin is added in the form of silk fibroin solution, silk peptide solution, and water-soluble silk peptide powder, so it can only play the role of nutritional additive.

Contents of the invention

The purpose of the present invention is to develop a preparation method of skin care silk fibroin gel cream.

The technical problem to be solved by the present invention is that the existing silk fibroin can only be used as a nutritional additive in cosmetic and skin care products, and the functions of moisturizing and matrix have not been fully exerted.

In the present invention, after the silk fibroin is made into a silk gel with a high water content, it is then made into a cosmetic skin care product and a skin repair product in the form of a cream. In these products, silk fibroin gel not only has the function of nutritional additives, but also has the function of moisturizing, and also acts as a matrix. It fully exerts the various effects of silk fibroin, can reduce the use of other synthetic raw materials, and obtain high-quality products. Natural beauty skin care products.

The main component of the silk fibroin gel in the present invention has the following characteristics: the silk fibroin gel made of 1 gram of silk fibroin can contain up to about 90 grams of water, and has high water retention. Silk fibroin gel contains high-molecular-weight silk fibroin, and also releases low-molecular-weight silk fibroin polypeptides and amino acids. The content of low-molecular-weight silk fibroin polypeptides and amino acids can also be increased during the preparation process as required. Low-molecular silk fibroin peptides, amino acids and moisture can penetrate directly into the skin to nourish skin cells. The high-molecular silk fibroin has a good affinity with the skin, and can form a microenvironment that moisturizes the skin on the surface of the skin. Silk fibroin is both hydrophilic and lipophilic. Hydrophilicity enables silk fibroin gel to hold a large amount of water and replenish moisture to the skin. The lipophilicity makes silk fibroin gel not only nourish the skin, but also gently remove excess facial oil.

These characteristics of silk fibroin gel can be used in cream-like skin repair and health care products after minor facial cosmetic operations such as facial mask cream, skin care cream, freckle removal, etc., and play a very good effect. For example: when using silk fibroin gel mask cream, apply a thin layer on the face and keep it moist for a certain period of time. At the beginning, it plays the role of nourishing the skin and replenishing skin moisture, and then the water in the silk fibroin gel gradually evaporates At the same time, the gel shrinks to tighten the skin and eliminate small wrinkles. The silk fibroin gel mask cream without added fatty substances is very easy to wash with water, and after washing, the facial skin is smooth, tender, clean and smooth. In addition, silk fibroin gel mask cream is applied on the face in a thin layer, which is easy to use and does not affect activities, so it is very suitable for office workers who pay attention to skin care and lack time. The skin after minor facial beauty operations such as freckle removal needs careful care. The natural quality silk fibroin gel skin repair and health care cream applied on the injured skin can take good care of the skin, promote skin growth, and make the restored skin smooth More natural and delicate.

Technical scheme of the present invention has following four kinds

The first technical solution includes the following steps:

(a) In the 10-200g/L silk fibroin aqueous solution, add water-soluble sericin 0-30g/L as a gel accelerator, after uniform mixing, let the solution stand at 0-90°C until The solution gels into a translucent silk fibroin gel;

(b) By adjusting the concentration of the silk fibroin aqueous solution, the water content of the silk fibroin gel is controlled within the range of 98%-70%; or the translucent silk fibroin gel prepared above is air-dried at room temperature or Evaporate water at 40-80°C to reduce the moisture content to 98%-70%;

(c) Homogenously emulsifying the prepared silk fibroin gel into a cream-like paste, adding an appropriate amount of water during this process to adjust the consistency of the cream;

(d) An appropriate amount of preservative can be added when preparing the silk fibroin gel as required.

The second technical solution includes the following steps:

(a) In 10-200g/L silk fibroin aqueous solution, add silk fibroin peptide 0-50g/L (molecular weight below 2000), add water-soluble sericin 0-30g/L as a gel accelerator, and mix evenly Finally, let the solution stand at 0-90°C until the solution gels into a translucent gel;

(b) adjust the concentration of the silk fibroin aqueous solution so that the water content of the silk fibroin gel is controlled within the range of 98%-70%; or air-dry the translucent silk fibroin gel prepared above at room temperature or Evaporate water at 40-80°C and reduce the moisture content to 98%-70%;

(c) homogeneously emulsifying the prepared silk fibroin gel into a creamy paste. During this process, an appropriate amount of water can be added to adjust the consistency of the cream;

(d) An appropriate amount of preservative can be added when preparing the silk fibroin gel as required.

The third technical solution includes the following steps:

(a) In the 10-200g/L silk fibroin aqueous solution, add silk fibroin peptide 0-50g/L (molecular weight below 2000), after adding other cosmetic base components and auxiliary components in an appropriate amount, add water-soluble sericin 0 -30g/L is used as a gel accelerator, after uniform mixing, the solution is allowed to stand at 0-90°C until the solution gels into a translucent gel;

(b) adjust the concentration of the silk fibroin aqueous solution so that the water content of the silk fibroin gel is controlled within the range of 98%-70%; or air-dry the translucent silk fibroin gel prepared above at room temperature or Evaporate water at 40-80°C and reduce the moisture content to 98%-70%;

(c) homogeneously emulsifying the prepared silk fibroin gel into a creamy paste. During this process, an appropriate amount of water can be added to adjust the consistency of the cream;

(d) An appropriate amount of preservative can be added when preparing the silk fibroin gel as required.

The fourth technical solution includes the following steps:

(a) After adding other cosmetic matrix components and auxiliary components into the gel formed by the silk fibroin gel obtained by the first technical scheme and the silk fibroin and silk peptide obtained by the second technical scheme, homogeneously emulsify into a creamy consistency.

Detailed ways

The present invention will be further described below in conjunction with the examples, but the present invention is not limited to the given examples.

Example 1: Silk Fibroin Gel Mask Cream

The silkworm cocoon is obtained by peeling the cocoon and removing the pupae to obtain the cocoon layer. Scouring the cocoon layer twice with 5g/L Na ₂ CO ₃ aqueous solution, 30 minutes each time, the scouring temperature is 98°C, and the bath ratio is 1:100. After scouring, it is fully washed with distilled water and dried naturally to obtain silk fiber. Dissolve the silk fiber with 1000g/L CaCl ₂ aqueous solution, the dissolution temperature is 98°C, the bath ratio is 1:25, and the time is 10 minutes. The solution obtained after dissolution was poured into a cellulose dialysis membrane (molecular component 12,000-14,000), and dialyzed in running water for 3 days. The dialyzed solution was air-dried and concentrated to obtain an aqueous silk fibroin solution. Add 5 g/L water-soluble sericin to the 50 g/L silk fibroin aqueous solution, and let it stand at 60°C until the solution becomes a translucent gel, or the gel is homogeneously emulsified into a cream. An appropriate amount of preservative can be added when preparing the silk fibroin gel as required.

The formula is simple, but what you get is a high-quality natural silk fibroin mask cream, which can give full play to the unique nourishing and moisturizing effects of silk fibroin, and has obvious moisturizing, rejuvenating, wrinkle-removing and cleansing effects on the facial skin. effect.

Embodiment two |, silk fibroin gel anti-aging facial mask cream

The silkworm cocoon is obtained by peeling the cocoon and removing the pupae to obtain the cocoon layer. Scouring the cocoon layer twice with 5g/L Na ₂ CO ₃ aqueous solution, 30 minutes each time, the scouring temperature is 98°C, and the bath ratio is 1:100. After scouring, it is fully washed with distilled water and dried naturally to obtain silk fiber. Dissolve the silk fiber with calcium chloride, water and ethanol solution with a molar ratio of 1:8:2, the dissolution temperature is 70°C, the bath ratio is 1:25, and the time is 10 minutes. The solution obtained after dissolution was poured into a cellulose dialysis membrane (molecular component 12,000-14,000), and dialyzed in running water for 3 days. The dialyzed solution was air-dried and concentrated to obtain an aqueous silk fibroin solution. Add 10g/L water-soluble sericin to 60g/L silk fibroin aqueous solution, add 10g/L silk fibroin peptide with a molecular weight below 2000, and let it stand at 70°C until the solution becomes a translucent gel. The gel is homogeneously emulsified together with other cosmetic auxiliary ingredients glycerin, vitamin E acetate, citrus essential oil, essence and preservatives to make an anti-aging mask cream.

The specific ratio is:

Component Mass fraction/%

Silk Fibroin Gel 60.0

Glycerin 2.0

Vitamin E acetate 0.5

Citrus Essential Oil 1.0

Preservatives Appropriate amount

Fragrance Appropriate amount

Add deionized water to 100.0

The preservatives can be benzoic acid, sorbic acid, salicylate, etc. of acid preservatives, parabens (A, B, C, butyl esters), paraben, parachloromethane, etc. of phenolic preservatives. Xylenol, etc., 2,4,4'-trichloro-N-carboxanilide of amides, alkyl trimethyl ammonium chloride, alkyl quinoline bromide, hexadecyl of quaternary ammonium salts Pyridine chloride, ethanol in alcohol preservatives, methyl dibromoglutaronitrile and phenoxyethanol in compound preservatives, bis-imidazolidinyl urea (GERMALL II) and iodine, you can choose one or more of them .

Example 3: silk fibroin gel nourishing face cream

The silkworm cocoon is obtained by peeling the cocoon and removing the pupae to obtain the cocoon layer. Scouring the cocoon layer 2 times with 0.4% neutral soap solution, 1 hour each time, scouring temperature 98°C, bath ratio 1:100. After scouring, it is fully washed with distilled water and dried naturally to obtain silk fiber. A ternary solvent mixed with lithium bromide, ethanol and water at a weight ratio of 45:53:2 is used to heat and dissolve silk fibers at a temperature of 70-100°C. Bath ratio 1:50. The solution obtained after dissolution was poured into a cellulose dialysis membrane (molecular component 12,000-14,000), and dialyzed in running water for 3 days. The dialyzed solution was air-dried and concentrated to obtain an aqueous silk fibroin solution. Add 30 g/L propylene glycol to the 80 g/L silk fibroin aqueous solution, and let it stand at 50° C. until the solution becomes a translucent gel. The gel is homogeneously emulsified with other cosmetic base ingredients stearic acid, beeswax, squalane, cetyl alcohol, polyoxyethylene monolaurate, auxiliary ingredients lanolin derivatives, flavors and preservatives to form a nourishing formula. cream. The specific ratio is:

Component Mass fraction/%

Silk Fibroin Gel 51.0

Stearic acid 5.0

Beeswax 2.0

Cetyl alcohol 6.0

Squalane 5.0

Polyoxyethylene monolaurate 3.0

Lanolin Derivatives 2.0

Fragrance Appropriate amount

Preservatives Appropriate amount

Add deionized water to 100.0

The preservative is selected to be the same as in Example 1.

Example 4: Silk Fibroin Gel Skin Repair and Health Care Cream

Obtain the silk fibroin aqueous solution with the method in embodiment one to three. Then, add 30 g/L ethanol to the 40 g/L silk fibroin aqueous solution, and let it stand at 60° C. until the solution becomes a translucent gel with a water content of about 90%. The gel is homogeneously emulsified together with other cosmetic base components vaseline, auxiliary components ethylene glycol, basic fibroblast growth factor (BFGF), vitamin E acetate and preservatives, and formulated as a skin repair and health care cream. The specific ratio is:

Component Mass Fraction/%

Silk Fibroin Gel 86.4

Vaseline 8.0

Ethylene glycol 5.0

Basic Fibroblast Growth Factor (BFGF) 0.1

Vitamin E acetate 0.5

Preservatives Appropriate amount

The preservative is selected to be the same as in Example 1.

Example 5: Silk Fibroin Gel Nutrient Emulsion

Obtain silk fibroin aqueous solution with embodiment one or embodiment two or embodiment three method. Then, in the 100g/L silk fibroin aqueous solution, add 10g/L silk fibroin peptide (molecular weight below 2000), add 10g/L water-soluble sericin as a gel accelerator, and mix the solution statically at 60°C. Place until the solution gels into a translucent gel. Homogenize the gel with other cosmetic base ingredients cetyl alcohol, white oil, fatty alcohol polyoxyethylene ether, glyceryl monostearate, sodium lauryl sulfate, auxiliary ingredients glycerin, ginsenoside, fragrance and preservatives Emulsifies to form a nourishing face cream. The specific ratio is:

Component Mass Fraction/%

Silk Fibroin Gel 20.0

Cetyl alcohol 2.0

White oil 8.5

Fatty alcohol polyoxyethylene ether 0.6

Glyceryl monostearate 3.0

Sodium Lauryl Sulfate 0.6

Ginsenosides 0.1

Glycerin 5.0

Fragrance Appropriate amount

Preservatives Appropriate amount

Add deionized water to 100.0

The preservative is selected to be the same as in Example 1.

Example 6: Silk Fibroin Gel Nutritious Mask

Obtain silk fibroin aqueous solution with embodiment one or embodiment two or embodiment three method. Then, in the 50g/L silk fibroin aqueous solution, add silk fibroin peptide 10g/L (molecular weight below 2000), add auxiliary components sodium alginate 5g/L, propylene glycol 20g/L, preservative amount, and mix the solution evenly at 20 Stand at ℃ until the solution gels into a translucent gel. The gel can also be homogeneously emulsified into a cream, and an appropriate amount of deionized water can be added to adjust the consistency.

The preservative is selected to be the same as in Example 1.

The water-soluble sericin in the present invention can be replaced by water-soluble chitosan as a gel accelerator; also water-soluble organic substance ethanol, betaine, polyethylene glycol which can be used for cosmetics without harmful stimulation to the skin can be used. Alcohol, ethylene glycol, propylene glycol, butylene glycol, polyvinylpyrrolidone, and one or more of their derivatives are used instead, and the amount of the gel accelerator used is 0-100g/L.

The matrix component of the present invention can also be palmitic acid, myristyl alcohol, oleyl alcohol, silicone oil and derivatives thereof.

The auxiliary component of the present invention can also be surfactant, lecithin, soap, pigment, peppermint oil, lavender oil, rose oil, brandy leaf oil, lactic acid and sodium lactate, sodium pyrrolidone carboxylate, basic fibroblast growth factor ( BFGF), fruit acids, enzymes (SOD) vitamins (A, B1, B2, B3, B6, C, D, F).

Claims (7)

1. a preparation method for skin care silk fibroin gel cream, is characterized in that it comprises the following steps: (a) In the 10-200g/L silk fibroin aqueous solution, add 5-30g/L water-soluble sericin as a gel accelerator, mix evenly, and let the solution stand at 0-90°C until the solution Gel into a translucent silk fibroin gel; the molecular weight of silk fibroin is 10,000-350,000; (b) By adjusting the concentration of the silk fibroin aqueous solution, the water content of the silk fibroin gel is controlled within the range of 98%-70%; or the translucent silk fibroin gel prepared above is air-dried at room temperature or Evaporate water at 40-80°C to reduce the moisture content to 98%-70%; (c) Homogenously emulsifying the prepared silk fibroin gel into a cream-like paste, adding an appropriate amount of water during this process to adjust the consistency of the cream; (d) adding an appropriate amount of preservatives when preparing silk fibroin gel; the preservatives are acid preservatives including benzoic acid, sorbic acid, salicylate; Parkin, p-chloro-m-xylenol; amide preservatives include 2,4,4'-trichloro-N-carboxanilide; quaternary ammonium salt preservatives include alkyltrimethylammonium chloride, alkyl Quinoline bromide, cetylpyridinium chloride; ethanol for alcohol preservatives; complex preservatives including methyl dibromoglutaronitrile and phenoxyethanol, diimidazolidinyl urea and iodine; add one of or several. 2. The preparation method of the skin care silk fibroin gel cream according to claim 1, is characterized in that after the silk fibroin gel is added with cosmetic matrix components and auxiliary components, it is homogeneously emulsified into a cream of a certain consistency thing. 3. A preparation method for skin care silk fibroin gel cream, characterized in that it comprises the following steps: (a) In 10-200g/L silk fibroin aqueous solution, add silk fibroin peptide 0-50g/L, the molecular weight of silk fibroin peptide is below 2000, add water-soluble sericin protein 10-30g/L as a gel accelerator After uniform mixing, the solution is left to stand at 0-90°C until the solution gels into a translucent gel; the molecular weight of silk fibroin is 10,000-350,000; (b) adjust the concentration of the silk fibroin aqueous solution so that the water content of the silk fibroin gel is controlled within the range of 98%-70%; or air-dry the translucent silk fibroin gel prepared above at room temperature or Evaporate water at 40-80°C and reduce the moisture content to 98%-70%; (c) homogeneously emulsifying the prepared silk fibroin gel into a cream; adding an appropriate amount of water in the process to adjust the consistency of the cream; (d) adding an appropriate amount of preservatives when preparing silk fibroin gel; the preservatives are acid preservatives including benzoic acid, sorbic acid, salicylate; Parkin, p-chloro-m-xylenol; amide preservatives include 2,4,4'-trichloro-N-carboxanilide; quaternary ammonium salt preservatives include alkyltrimethylammonium chloride, alkyl Quinoline bromide, cetylpyridinium chloride; ethanol for alcohol preservatives; complex preservatives including methyl dibromoglutaronitrile and phenoxyethanol, diimidazolidinyl urea and iodine; add one of or several. 4. The preparation method of skin care silk fibroin gel cream according to claim 3 is characterized in that in the gel formed by silk fibroin and silk peptide, after adding cosmetic matrix components and auxiliary components, homogeneous emulsification into a creamy consistency. 5. according to the preparation method of the described skin care silk fibroin gel cream of claim 2 or 4, it is characterized in that the matrix component added comprises stearic acid, palmitic acid, myristyl alcohol, oleyl alcohol, beeswax, squalane, Lanolin and its derivatives, cetyl alcohol, polyoxyethylene monolaurate, sodium alginate, petrolatum, white oil, fatty alcohol polyoxyethylene ether, glyceryl monostearate, sodium lauryl sulfate, silicone oil And its derivatives, propylene glycol, auxiliary ingredients include lecithin, soap, essence, pigment, peppermint oil, lavender oil, rose oil, brandy leaf oil, glycerin, propylene glycol, 1,3-butanediol, polyethylene glycol, lactic acid , vitamin E acetate, citrus essential oil, ethylene glycol, basic fibroblast growth factor, fruit acid, enzyme, ginsenoside, seaweed extract, vitamin A, B1, B2, B3, B6, C, D, F . 6. The preparation method of the skin care silk fibroin gel cream according to claim 1 or 3, characterized in that the preparation method of the silk fibroin solution is: add water with commonly used calcium chloride solution, lithium bromide solution, or calcium chloride The silk fibroin is dissolved by adding ethanol mixed solution, and then obtained by dialysis and concentration. 7. The preparation method of the skin care silk fibroin gel cream according to claim 1 or 3, characterized in that water-soluble sericin is also a protein extracted from silk.