CN1985791A

CN1985791A - Preparing process of skin care silk protein gel cream

Info

Publication number: CN1985791A
Application number: CN 200610155515
Authority: CN
Inventors: 闵思佳; 吴迪
Original assignee: Zhejiang University ZJU
Current assignee: Hangzhou Wensli Silk Culture Co ltd
Priority date: 2006-12-27
Filing date: 2006-12-27
Publication date: 2007-06-27
Anticipated expiration: 2026-12-27
Also published as: CN1985791B

Abstract

本发明公开了一种护肤丝素凝胶霜膏的制备方法，其制备步骤包括：在丝素蛋白水溶液中，加入水溶性丝胶蛋白或水溶性丝胶蛋白和丝素肽作为凝胶促进剂，均匀混合后，将溶液在0－90℃条件下静置，至溶液凝胶化成为半透明状丝素凝胶，丝素蛋白凝胶的含水率控制在98％－70％范围内，将制成的丝素蛋白凝胶匀质乳化成霜膏状。根据需要在制备步骤中可适量加入其他的化妆品基质成分和辅助成分。由于本发明中的丝素凝胶既有营养添加剂作用、又有保湿的作用、还起到基质的作用，所以用本发明可生产出高品质的天然美容护肤制品。The invention discloses a preparation method of silk gel cream for skin care. The preparation step comprises: adding water-soluble sericin or water-soluble sericin and silk peptide as a gel accelerator to silk fibroin aqueous solution After uniform mixing, the solution is left standing at 0-90°C until the solution gels into a translucent silk fibroin gel, and the water content of the silk fibroin gel is controlled within the range of 98%-70%. The prepared silk fibroin gel is homogeneously emulsified into a cream. According to needs, other cosmetic base components and auxiliary components can be added in an appropriate amount in the preparation step. Because the silk fibroin gel in the present invention not only has the function of nutrition additive, but also has the function of moisturizing and matrix, so the present invention can be used to produce high-quality natural beauty and skin care products.

Description

The preparation method of skin care silk protein gel cream

Technical field

The present invention relates to a kind of preparation method that is used for beauty and skin care, repairs the fibroin albumen gel cream of skin.

Background technology

Along with growth in the living standard, people more and more pay attention to beauty and skin care, and are also more and more higher to the quality requirements of cosmetics and skincare product.Contain the cosmetics and skincare product of the active skull cap components that extracts in the biologies such as collagen protein, fibroin albumen, seaweed extract thing, Radix Ginseng leaching liquid, not only protect skin, also have the function of conditioning skin, promotion skin metabolism, enjoy favor.Silkworm silk and human body skin have good affinity, and ventilative suction is described as the fiber queen since ancient times.Fibroin albumen is to extract the native protein that obtains from silkworm silk, is made up of 18 seed amino acids.Studies show that fibroin albumen has the biological activity that promotes growth to most mammal cells such as fibroblast, epidermis cell, epithelial cell, chondrocytes, so fibroin albumen is applied in the medical materials such as various cosmetics and skincare products and artificial skin.

Usually fibroin albumen only plays the effect of nourishing additive agent, and does not bring into play moisture-keeping functions in cosmetics and skincare product, does not also have the function of substrate, that is to say that the effect of fibroin albumen is not fully exerted.Its reason is because fibroin albumen is the form adding with silk fibroin protein solution, silk peptide solution, water solublity silk peptide powder, so it only can bring into play the effect of nourishing additive agent.

Summary of the invention

The objective of the invention is to develop a kind of preparation method of skin care silk protein protein gel cream.

The technical problem to be solved in the present invention be existing fibroin albumen in cosmetics and skincare product, fibroin albumen can only use as nourishing additive agent, preserves moisture and problem that the substrate effect is not fully exerted.

Among the present invention, fibroin albumen is made the silk extract gel of high-moisture percentage after, make beauty and skin care goods, the skin repair goods of cream form again.Silk extract gel existing nourishing additive agent effect in these goods, again have the effect of preserving moisture, also play the effect of substrate, given full play to the multiple efficacies of fibroin albumen, can reduce the use of other synthesis materials, obtain high-quality natural beauty and skin care goods.

Main Ingredients and Appearance silk extract gel among the present invention has such characteristic: the silk extract gel that 1 gram fibroin albumen is made can contain up to the moisture about 90 grams, and very high water-retaining property is arranged.Contain high-molecular weight fibroin albumen in the silk extract gel, also can dissociate low-molecular-weight fibroin albumen polypeptide and aminoacid can also increase low-molecular-weight fibroin albumen polypeptide and amino acid whose content as required in preparation process.Low molecular fibroin albumen polypeptide, aminoacid and moisture can directly infiltrate skin, nourishes rough skin cell.High molecular fibroin albumen and skin have good affinity, can form the microenvironment of skin care at skin surface.Fibroin albumen promptly has hydrophilic, and lipotropy is also arranged.Hydrophilic makes the fibroin albumen gel can contain large quantity of moisture, for skin keeps the skin wet.Lipotropy makes the fibroin albumen gel also have the soft facial unnecessary greasy effect of removing except nourishes rough skin.

Aspects such as cream shape skin repair health product after these characteristics of silk extract gel make it can be applied in beautifying face minor operations such as face mask cream, skin cream, speckle dispelling play good effect.For example: be applied on the face with skim when the silk extract gel face mask cream uses, and keeping certain moistening time on the face, play the effect of skin nutrition and additional moisture of skin during beginning, back gel when silk extract gel moisture progressively evaporates shrinks, and plays the effect of Firm and the little wrinkle of elimination.The silk extract gel face mask cream that does not add the fat added material is very easy to water and cleans, and cleans the bright and clean delicacy of rear face skin, cleaning is smooth.The silk extract gel face mask cream is to be applied on the face with skim in addition, and is easy to use, do not influence activity again, so very suitable emphasis skin care lacks the working clan of time again.Skin after the beautifying face minor operations such as speckle dispelling needs careful care, and the silk extract gel skin repair health care cream of natural quality is coated on the injured skin can take good care of skin well, promotes skin growth, makes the delicacy more naturally that becomes of skin after the recovery.

Technical scheme of the present invention has following four kinds

First kind of technical scheme may further comprise the steps:

(a) in the silk fibroin water solution of 10-200g/L, adding water-soluble silk glue albumen 0-30g/L, leaves standstill solution behind the uniform mixing as gel promoter under 0-90 ℃ of condition, changes into to solution gel to be translucent silk extract gel;

(b) by adjusting the concentration of silk fibroin water solution, the moisture content of fibroin albumen gel is controlled in the 98%-70% scope; Or air-dry at normal temperatures or the above-mentioned translucent silk extract gel that is prepared at 40-80 ℃ of following transpiring moisture, reduce moisture content to the 98%-70% scope;

(c) the even matter of the fibroin albumen gel of making is emulsified into the cream shape, in this process, can adds amount of water, to adjust the denseness of cream;

(d) can when the preparation silk extract gel, add an amount of antiseptic as required.

Second kind of technical scheme may further comprise the steps:

(a) in the silk fibroin water solution of 10-200g/L, add silk polypeplide 0-50g/L (molecular weight is below 2000), add water-soluble silk glue albumen 0-30g/L as gel promoter, behind the uniform mixing solution is left standstill under 0-90 ℃ of condition, change into translucent gel to solution gel;

(b) concentration of adjustment silk fibroin water solution is controlled in the 98%-70% scope moisture content of fibroin albumen gel; Or air-dry at normal temperatures or the above-mentioned translucent silk extract gel that is prepared at 40-80 ℃ of following transpiring moisture, reduce moisture content to the 98%-70% scope;

(c) the even matter of the fibroin albumen gel of making is emulsified into the cream shape.In this process, can add amount of water, to adjust the denseness of cream;

The third technical scheme may further comprise the steps:

(a) in the silk fibroin water solution of 10-200g/L, add silk polypeplide 0-50g/L (molecular weight is below 2000), after adding other cosmetic base composition and auxiliary element in right amount, add water-soluble silk glue albumen 0-30g/L as gel promoter, behind the uniform mixing solution is left standstill under 0-90 ℃ of condition, change into to solution gel and be translucent gel;

(b) concentration of adjustment silk fibroin water solution is controlled in the 98%-70% scope moisture content of fibroin albumen gel; Or air-dry at normal temperatures or the above-mentioned translucent silk extract gel that is prepared at 40-80 ℃ of following transpiring moisture, reduce moisture content to the 98%-70% scope:

The 4th kind of technical scheme may further comprise the steps:

(a) in the gel that fibroin albumen that fibroin albumen gel that first kind of technical scheme obtains and second kind of technical scheme obtain and silk polypeplide form, add other cosmetic base composition and auxiliary element after, even matter is emulsified into the white paste of certain denseness.

The specific embodiment

The invention will be further described below in conjunction with embodiment, but the present invention is not confined to given embodiment.

Embodiment one: the silk extract gel face mask cream

Family's Bombyx bombycis is through shelling cocoon, going pupa to obtain cocoon layer.Use 5g/L Na ₂CO ₃The concise cocoon layer of aqueous solution 2 times, each 30 minutes kinds, 98 ℃ of concise temperature, bath raio 1: 100.Concise back is fully clean with distilled water, and natural drying obtains fibroin fiber.Use 1000g/L CaCl ₂Aqueous solution dissolving fibroin fiber, 98 ℃ of solution temperatures, bath raio 1: 25,10 minutes time.The solution that obtains after the dissolving is poured in the cellulose dialyzer (molecular components 12,000～14,000), and dialysis is 3 in flowing water.Solution after the dialysis is air-dry concentrated, obtain silk fibroin water solution.In the silk fibroin water solution of 50g/L, add water-soluble silk glue albumen 5g/L, leave standstill under 60 ℃ of conditions, become translucent gel to solution, perhaps the even matter of gel is emulsified into the cream shape.Can when the preparation silk extract gel, add an amount of antiseptic as required.

This prescription is simple, but what obtain is high-quality natural silk fibroin face mask cream, can give full play to distinctive nourishing effect of fibroin albumen and moisture-keeping efficacy, to skin of face have that tangible benefit is wet, the effect of skin care, reduce wrinkle, cleaning.

Embodiment two |, silk extract gel anti-ageing face mask cream

Family's Bombyx bombycis is through shelling cocoon, going pupa to obtain cocoon layer.Use 5g/L Na ₂CO ₃The concise cocoon layer of aqueous solution 2 times, each 30 minutes kinds, 98 ℃ of concise temperature, bath raio 1: 100.Concise back is fully clean with distilled water, and natural drying obtains fibroin fiber.With mol ratio is 1: 8: 2 calcium chloride, water, dissolve with ethanol solution fibroin fiber, 70 ℃ of solution temperatures, bath raio 1: 25,10 minutes time.The solution that obtains after the dissolving is poured in the cellulose dialyzer (molecular components 12,000～14,000), and dialysis is 3 in flowing water.Solution after the dialysis is air-dry concentrated, obtain silk fibroin water solution.In the 60g/L silk fibroin water solution, add water-soluble silk glue protein 10 g/L, add the silk polypeplide 10g/L of molecular weight below 2000, leave standstill under 70 ℃ of conditions, become translucent gel to solution.Gel with other cosmetics auxiliary element glycerol, vitamin E acetic acid fat, foreign Citrus essential oil, essence and antiseptic, even matter emulsifying, is made into anti-ageing face mask cream.

Concrete proportioning is:

Constituent mass mark/%

Silk extract gel 60.0

Glycerol 2.0

Vitamin E acetic acid fat 0.5

Ocean Citrus essential oil 1.0

Antiseptic is an amount of

Essence is an amount of

Deionized water adds to 100.0

Antiseptic can be benzoic acid, sorbic acid, Salicylate of acids antiseptic etc., the parabens of antiseptic phenols (first, second, third, butyl ester), nipalgin, parachlorometaxylenol etc., 2 of amide-type, 4,4 '-three chloros-carbanilide, the alkyl trimethyl ammonium chloride of quaternary ammonium salt, alkyl bromination quinoline, hexadecylpyridinium chloride, the ethanol of alcohols antiseptic etc., the methyl dibromo glutaronitrile of composite anti-corrosion agent and phenoxyethanol, two imidazolidinyl urea (GERMALL II) and iodine can be selected wherein one or more for use.

Embodiment three: silk extract gel nourishes facial cream

Family's Bombyx bombycis is through shelling cocoon, going pupa to obtain cocoon layer.With the concise cocoon layer of 0.4% neutral soap solution 2 times, each 1 hour, 98 ℃ of concise temperature, bath raio 1: 100.Concise back is fully clean with distilled water, and natural drying obtains fibroin fiber.With lithium bromide, ethanol, water with the composite 3 yuan of solvents of 45: 53: 2 weight ratio, heating for dissolving fibroin fiber under 70-100 ℃ of temperature.Bath raio 1: 50.The solution that obtains after the dissolving is poured in the cellulose dialyzer (molecular components 12,000～14,000), and dialysis is 3 in flowing water.Solution after the dialysis is air-dry concentrated, obtain silk fibroin water solution.In the silk fibroin water solution of 80g/L, add propylene glycol 30g/L, leave standstill under 50 ℃ of conditions, become translucent gel to solution.With gel with other cosmetic base composition stearic acid, Cera Flava, squalane,, hexadecanol, Vinlub 73, auxiliary element lanolin derivative, essence and the even matter emulsifying of antiseptic, be made into the nourishing facial cream.Concrete proportioning is:

Constituent mass mark/%

Silk extract gel 51.0

Stearic acid 5.0

Cera Flava 2.0

Hexadecanol 6.0

Squalane 5.0

Vinlub 73 3.0

Lanolin derivative 2.0

Essence is an amount of

Antiseptic is an amount of

Deionized water adds to 100.0

It is identical with embodiment one that antiseptic is selected for use.

Embodiment four: silk extract gel skin repair health-care cream

Obtain silk fibroin water solution with the method among the embodiment one to three.In the silk fibroin water solution of 40g/L, add ethanol 30g/L then, leave standstill under 60 ℃ of conditions, become the translucent gels of moisture content about 90% to solution.Gel with the even matter emulsifying of other cosmetic base composition vaseline, auxiliary element ethylene glycol, basic fibroblast growth factor (BFGF), vitamin E acetic acid fat and antiseptic, is made into the skin repair health-care cream.Concrete proportioning is:

Constituent mass mark/%

Silk extract gel 86.4

Vaseline 8.0

Ethylene glycol 5.0

Basic fibroblast growth factor (BFGF) 0.1

Vitamin E acetic acid fat 0.5

Antiseptic is an amount of

It is identical with embodiment one that antiseptic is selected for use.

Embodiment five: the silk extract gel nutritional emulsions

Obtain silk fibroin water solution with embodiment one or embodiment two or embodiment three methods.Then in the 100g/L silk fibroin water solution, add silk polypeplide 10g/L (molecular weight is below 2000), add water-soluble silk glue protein 10 g/L as gel promoter, behind the uniform mixing solution is left standstill under 60 ℃ of conditions, change into translucent gel to solution gel.Gel with the even matter emulsifying of other cosmetic base composition hexadecanol, white oil, fatty alcohol-polyoxyethylene ether, glyceryl monostearate, sodium lauryl sulphate, auxiliary element glycerol, ginsenoside, essence and antiseptic, is made into the nourishing facial cream.Concrete proportioning is:

Constituent mass mark/%

Silk extract gel 20.0

Hexadecanol 2.0

White oil 8.5

Fatty alcohol-polyoxyethylene ether 0.6

Glyceryl monostearate 3.0

Sodium lauryl sulphate 0.6

The ginsenoside 0.1

Glycerol 5.0

Essence is an amount of

Antiseptic is an amount of

Deionized water adds to 100.0

It is identical with embodiment one that antiseptic is selected for use.

Embodiment six: the silk extract gel nourishing pack

Obtain silk fibroin water solution with embodiment one or embodiment two or embodiment three methods.Then in the 50g/L silk fibroin water solution, add silk polypeplide 10g/L (molecular weight is below 2000), add auxiliary element sodium alginate 5g/L, propylene glycol 20g/L, antiseptic is an amount of, behind the uniform mixing solution is left standstill under 20 ℃ of conditions, change into to solution gel to be translucent gel.Also the even matter of gel can be emulsified into the cream shape, add deionized water in right amount and regulate denseness.

It is identical with embodiment one that antiseptic is selected for use.

Water-soluble silk glue albumen among the present invention can replace with water-soluble chitosan as gel promoter; Also can be with the water soluble organic substance ethanol that can be used for cosmetics that skin is not had the destructive stimulus effect, trimethylammonium second inner salt, Polyethylene Glycol, ethylene glycol, propylene glycol, butanediol, polyvinyl pyrrolidone, and one or more replacements in the derivant, the use amount of gel promoter is 0-100g/L.

Matrix components of the present invention can also be palmitic acid, cardanol, oleyl alcohol, silicone oil and derivant thereof.

Auxiliary element of the present invention can also be that surfactant, lecithin, soap, pigment, Oleum menthae, Oleum lavandula angustifolia, Oleum Rosae Rugosae, Michelia leap oil, lactic acid and lactic acid are received, pyrrolidone sodium carboxylate, basic fibroblast growth factor (BFGF), fruit acid, enzyme (SOD) vitamin (A, B1, B2, B3, B6, C, D, F).

Claims

1, a preparation method of skin care silk fibroin gel cream, is characterized in that it comprises the following steps:

(a) In 10-200g/L silk fibroin aqueous solution (molecular weight 10,000-350,000), add water-soluble sericin 0-30g/L as a gel accelerator, after uniform mixing, put the solution at 0-90 Stand at ℃ until the solution gels into translucent silk fibroin gel;

(b) By adjusting the concentration of the silk fibroin aqueous solution, the water content of the silk fibroin gel is controlled within the range of 98%-70%; or the translucent silk fibroin gel prepared above is air-dried at room temperature or Evaporate water at 40-80°C to reduce the moisture content to 98%-70%;

(c) Homogenously emulsifying the prepared silk fibroin gel into a cream-like paste, adding an appropriate amount of water during this process to adjust the consistency of the cream;

(d) An appropriate amount of preservative can be added when preparing the silk fibroin gel as required.

2. The preparation method of skin care silk fibroin gel cream according to claim 1, characterized in that the silk fibroin gel is homogeneously emulsified into a cream with a certain consistency after adding other cosmetic matrix components and auxiliary components. paste.

3. A preparation method for skin care silk fibroin gel cream, characterized in that it comprises the following steps:

(a) In 10-200g/L silk fibroin aqueous solution (molecular weight 10,000-350,000), add silk fibroin peptide 0-50g/L (molecular weight below 2000), add water-soluble sericin 0-30g/L as Gel accelerator, after mixing evenly, let the solution stand at 0-90°C until the solution gels into a translucent gel;

(b) adjust the concentration of the silk fibroin aqueous solution so that the water content of the silk fibroin gel is controlled within the range of 98%-70%; or air-dry the translucent silk fibroin gel prepared above at room temperature or Evaporate water at 40-80°C and reduce the moisture content to 98%-70% range;

(c) homogeneously emulsifying the prepared silk fibroin gel into a creamy paste. During this process, an appropriate amount of water can be added to adjust the consistency of the cream;

4. The preparation method of skin care silk fibroin gel cream according to claim 3, characterized in that after adding other cosmetic matrix components and auxiliary components into the gel formed by silk fibroin and silk peptide, The substance is emulsified into a cream-like substance with a certain consistency.

5. A preparation method for skin care silk fibroin gel cream, characterized in that it comprises the following steps:

(a) In 10-200g/L silk fibroin aqueous solution (molecular weight 10,000-350,000), add silk fibroin peptide 0-50g/L (molecular weight below 2000), and add other cosmetic base ingredients and auxiliary ingredients in an appropriate amount, Add water-soluble sericin 0-30g/L as a gel accelerator, mix evenly and let the solution stand at 0-90°C until the solution gels into a translucent gel;

(b) adjust the concentration of the silk fibroin aqueous solution so that the water content of the silk fibroin gel is controlled within the range of 98%-70%; or air-dry the translucent silk fibroin gel prepared above at room temperature or Evaporate water at 40-80°C and reduce the moisture content to 98%-70%;

(c) homogeneously emulsifying the prepared silk fibroin gel into a cream. Add water during this process to adjust the consistency of the cream;

6. The preparation method of the skin care silk fibroin gel cream according to claim 1, 3 or 5, characterized in that the preservatives can be acid preservatives such as benzoic acid, sorbic acid, salicylate, phenolic preservatives parabens (A, B, C, butyl), p-chloro-m-xylenol, amides, 2,4,4'-trichloro-N-carbanilide, quaternary ammonium salts Alkyl trimethyl ammonium chloride, alkyl quinoline bromide, cetyl pyridinium chloride, ethanol for alcohol preservatives, methyl dibromoglutaronitrile and phenoxyethanol for compound preservatives, bis One or more of imidazolidinyl urea (GERMALL II) and iodine can be selected.

7. The preparation method of the skin care silk fibroin gel cream according to claim 2, 4 or 5, characterized in that other matrix components including stearic acid, palmitic acid, myristyl alcohol, oleyl alcohol, beeswax, horn Squalane, lanolin and its derivatives, cetyl alcohol, polyoxyethylene monolaurate, sodium alginate, petrolatum, white oil, fatty alcohol polyoxyethylene ether, glyceryl monostearate, lauryl sulfate Sodium, silicone oil and its derivatives, propylene glycol, auxiliary ingredients include surfactants, lecithin, soap, fragrance, pigment, peppermint oil, lavender oil, rose oil, brandy leaf oil, glycerin, propylene glycol, 1,3-butanediol , polyethylene glycol, lactic acid and sodium lactate, sodium pyrrolidone carboxylate, vitamin E acetate, citrus essential oil, ethylene glycol, basic fibroblast growth factor (BFGF), fruit acid, enzyme (SOD) ginsenoside, Seaweed extract, vitamins (A, B1, B2, B3, B6, C, D, F) preservatives, fragrance.

8. The preparation method of skin care silk fibroin gel box cream according to claim 1, 3 or 5, characterized in that the preparation method of silk fibroin solution is: use commonly used calcium chloride solution, lithium bromide solution, or chloride It is obtained by dissolving silk fibroin in a mixed solution of calcium, water and ethanol, followed by dialysis and concentration.

9. The method for preparing skin care silk fibroin gel cream according to claim 1, 3 or 5, characterized in that water-soluble sericin is also a protein extracted from silk.

10. The preparation method of skin care silk fibroin gel cream according to claim 1, 3 or 5, characterized in that water-soluble sericin can be replaced by water-soluble chitosan as a gel accelerator; One or more of the water-soluble organic substances ethanol, betaine, polyethylene glycol, ethylene glycol, propylene glycol, butylene glycol, polyvinylpyrrolidone, and their derivatives that can be used in cosmetics without harmful irritating effects Instead, the amount of the gel accelerator used is 0-100g/L.