CN111366429A - Smear preparation device and smear preparation method - Google Patents

Abstract

The invention is applicable to the technical field of medical equipment, and discloses a smear preparation device and a smear preparation method. The smear preparation device includes a slide loading module, a slide cart and a slide processing module; the slide loading module is used for loading the slides to be processed on the slide cart; the slide cart is used for placing the slides on the slide cart; The slides are transported to the slide processing module; the slide processing module includes a printing module, a sample drop module and a slide push module, and the slide cart transports the slides from the slide loading module They are respectively transported to the printing module, the dripping module and the slide pushing module, the printing module is used to print information on the slide, and the dripping module is used to drip the slide In the sample operation, the slide slide module is used to perform sample smoothing on the glass slide. In the present invention, the slides are transported to each station of the slide processing module by one clamping of the slide cart, and the clamping operation is simple and reliable.

Description

Smear preparation device and smear preparation method

technical field

The invention belongs to the technical field of medical equipment, and particularly relates to a smear preparation device, a smear preparation method and a slide basket drying module.

Background technique

At present, the fully automatic preparation instrument for blood smear or other tissue smear, as a smear preparation device, is generally divided into three important functions: preparation, pushing and staining. In the prior art, some smear preparation devices divide the two functions of smearing and dyeing into two instruments to complete, wherein after the blood smear is pushed, manual participation is required, and the blood smear that has been pushed is transferred. into the stainer. The manual transfer process not only increases labor costs but also increases the risk of blood smear damage, which easily affects the accuracy of the diagnostic results.

SUMMARY OF THE INVENTION

The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, and to provide a smear preparation device, a smear preparation method and a slide basket drying module, which have high reliability for preparing smears and are conducive to ensuring the accuracy of diagnostic results.

The technical scheme of the present invention is: a smear preparation device, comprising a sampling module for taking samples, a slide loading module for moving the slide to a working line, a printing module for printing information on the slide, Dropping module for dropping samples on glass slides, slide pushing module for smoothing samples on glass slides, inversion module for turning the slides over, and staining for staining the slides Modules, the slide loading module, the printing module, the sample drop module, the slide slide module, the inversion module and the dyeing module are arranged along the working line.

Specifically, the smear preparation device further includes a cleaning module for cleaning the glass slide, and the cleaning module is arranged along the working line.

Specifically, the smear preparation device further includes a slide basket drying module for drying the samples on the glass slide, and the slide basket drying module is arranged along the working line.

Specifically, the smear preparation device further includes a slide output module for carrying the slide basket and outputting the slide basket to a set position.

Specifically, the smear preparation device further includes a slide basket drying module for drying the stained slides.

Specifically, the sample dropping module includes a blood dropping needle, and the blood dropping needle is connected to the sampling module; one side of the blood dropping needle is provided with a micro inlet for moving the priority sample to the lower part of the blood dropping needle. sample module.

Specifically, the blood dripping needle includes a needle body and a guide sleeve, and an elastic structure is provided between the needle body and the guide sleeve.

Specifically, the working line includes a first working line and a second working line; the slide loading module, cleaning module, printing module, sample dropping module, slide pushing module and turning module are arranged along the first working line, The turning module is located at the intersection of the first working line and the second working line; the slide basket drying module and the staining module are arranged along the second working line.

Specifically, the first working line is provided with a first linear slide rail, and a slide cart for carrying glass slides is slidably connected to the first linear slide rail; the second working line is provided with a second straight line A slide rail, the second linear slide rail is slidably connected with a manipulator module for clamping and moving glass slides; the first working line and the second working line are vertically intersected or linearly connected.

Specifically, the smear preparation device further includes a sample automatic sampling module, and the sample automatic sampling module is disposed adjacent to the sampling module; the sample automatic sampling module includes a test tube rack feeding part, a sample mixing part part.

Specifically, the slide loading module includes a slide storage sub-module for accommodating slides, a cutter head sub-module for pushing the slides from the slide storage sub-module to the working line, Or the in-position blocking sub-module that is limited by the cutter head sub-module.

Specifically, the slide slide module includes a slide slide cutter head sub-module for smoothing the sample, and a flip module for flipping the slide from flat to upright.

Specifically, the smear preparation device has a control part for controlling the manipulator to grip and move the slides manually inserted into the slide basket drying module to the staining module.

Specifically, the staining module includes a turntable sub-module, a needle-disc sub-module and a staining box; the staining module has a slide entry station and a slide take-out station, the slide entry station, the slide take-out station, The inversion module, the slide basket drying module, and the slide output module are arranged in the same straight line.

Specifically, the slide basket drying module includes a casing, and the casing is provided with a blowing port for blowing out airflow and an air suction port for sucking in airflow.

The present invention also provides a glass slide basket drying module, which includes a casing, and the casing is provided with a blowing port for blowing out airflow and an air suction port for sucking in airflow.

The present invention also provides a method for preparing a smear, comprising the following steps: laying the glass slide flat and moving the glass slide along the working line; during the movement of the glass slide along the working line, cleaning the glass slide through a cleaning module. Clean, then print the sample information on the glass slide through the printing module, drop the sample collected from the sampling module on the glass slide through the sample drop module, and then smooth the sample on the glass slide through the slide push module, Then, the glass slide is turned from the flat state to the upright state by the turning module, and the turned glass slide is dyed by the staining module.

Specifically, in the process of inverting the glass slide from a flat state to an upright state, the flipping module flips the glass slide to tilt and stays for a set period of time.

Specifically, after the sample is printed on the glass slide and before the sample is dropped on the glass slide, the sample information printed on the glass slide is checked by the detection module.

In the smear preparation device, smear preparation method and slide basket drying module provided by the present invention, the slide loading module, printing module, sample dropping module, slide pushing module, turning module and dyeing module are arranged along the working line, The smear preparation device has a compact structure, and has high smear preparation efficiency and high reliability of the smear preparation, which is beneficial to ensure the accuracy of the diagnosis results.

Description of drawings

In order to illustrate the technical solutions in the embodiments of the present invention more clearly, the following briefly introduces the drawings required in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained from these drawings without any creative effort.

Fig. 1 is the three-dimensional assembly schematic diagram of the smear preparation device provided by the embodiment of the present invention;

Fig. 2 is the three-dimensional assembly schematic diagram of the smear preparation device provided by the embodiment of the present invention;

Fig. 3 is the arrangement schematic diagram of each station in the working line of the smear preparation device provided by the embodiment of the present invention;

4 is a schematic diagram of a three-dimensional assembly of a slide loading module in a smear preparation device provided by an embodiment of the present invention;

5 is a schematic plan view of a slide-pulling module in a smear preparation device provided by an embodiment of the present invention;

Fig. 6 is the three-dimensional assembly schematic diagram of the push-piece module in the smear preparation device provided by the embodiment of the present invention;

7 is a schematic diagram of a three-dimensional assembly of a slide basket drying module in a smear preparation device provided in an embodiment of the present invention;

8 is a schematic three-dimensional assembly diagram of a second slide rail and a manipulator module in a smear preparation device provided by an embodiment of the present invention;

9 is a schematic diagram of a three-dimensional assembly of a dyeing module in a smear preparation device provided by an embodiment of the present invention;

10 is a schematic diagram of a three-dimensional assembly of a middle slide basket drying module provided by an embodiment of the present invention;

FIG. 11 is a schematic three-dimensional assembly diagram of a middle slide output module provided by an embodiment of the present invention.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

It should be noted that when an element is referred to as being "fixed to" or "disposed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or intervening elements may also be present.

It should also be noted that the azimuth terms such as left, right, upper and lower in this embodiment are only relative concepts or refer to the normal use state of the product, and should not be regarded as limiting.

As shown in FIGS. 1 to 6 , a smear preparation device provided in an embodiment of the present invention can be used for smear preparation of samples such as blood and body fluids. The above-mentioned smear preparation device includes a sampling module 1 for taking samples, a slide loading module 3 for moving the slides to the working line, a printing module 4 for printing information on the slides, and a sample droplet on the slide. A drop sample module 5 for a glass slide, a slide slide module 6 for smoothing the sample on the glass slide, an inversion module 64 for turning the glass slide over (not shown in the figure), and a slide slide module 64 for flipping the glass slide The dyeing module 9 for dyeing, the slide loading module 3, the printing module 4, the sample drop module 5, the slide slide module 6, the inversion module 64 and the dyeing module 9 are arranged along the working line, and the structure is compact, and the smear is compact. The preparation efficiency is high, and the reliability of preparing the smear is high, which is beneficial to ensure the accuracy of the diagnosis result. There are various stations on the working line. The sampling module 1 is the sampling station, the slide loading module 3 is the glass loading station, the printing module 4 is the printing station, and the sample dropping module 5 is the printing station. The blood dripping station, the film pushing module 6 is the film pushing station, the turning module 64 is the turning station, and the dyeing module 9 is the dyeing station. It should be noted that, the glass slides in this embodiment are not made of glass material, but other suitable materials can also be used, which all belong to the protection scope of the present invention.

Specifically, the smear preparation device further includes a cleaning module (not shown in the figure) for cleaning the glass slide, the cleaning module is arranged along the working line, and the cleaning module is the cleaning station. In the step before the dripping module 5 performs blood dripping, for example, before printing the sample information, the glass slide can be cleaned by the cleaning module, for example, cleaning can be performed by blowing air.

Specifically, as shown in FIGS. 1 to 3 , the smear preparation device further includes a slide basket drying module 8 for drying the samples on the glass slide, and the slide basket drying module 8 can be used for drying samples such as blood films. . The slide basket drying module 8 is arranged along the working line, and the slide basket drying module 8 is the slide drying station, so that the blood sample can be dried in time.

Specifically, as shown in FIGS. 1 to 3 , the smear preparation device further includes a slide output module 10 for carrying a slide basket and outputting the slide basket to a set position. The slide output module 10 It is the slide output station.

Specifically, as shown in FIGS. 1 to 3 , the smear preparation device further includes a slide basket drying module for drying the stained slides. The slide basket drying module 12 is the slide basket drying module. Station.

Specifically, as shown in FIGS. 1 to 3 , the sample dropping module 5 includes a blood dropping needle 501 connected to the sampling module 1 ; one side of the blood dropping needle 501 is provided with a Move the priority sample to the micro injection module 2 below the blood drop needle 501 . The micro-injection module 2 can move the test tube put in by the operator directly in the direction of the blood drop needle 501, and at the same time, the blood drop needle 501 can also move in the direction of the test tube put in by the operator, because there is no need to draw blood through the sampling module 1 , which can reduce the need for blood samples, thereby enabling trace and priority injection. Moreover, the same blood drop needle 501 can be shared for batch injection and micro injection and priority injection.

Specifically, as shown in FIGS. 1 to 3 , the blood-dropping needle 501 includes a needle body and a guide sleeve, and an elastic structure is arranged between the needle body and the guide sleeve to prevent the blood-dropping needle 501 from rigidly hitting the glass. piece.

Specifically, as shown in FIGS. 1 to 6 , the working line includes a first working line 101 and a second working line 102 ; the slide loading module 3 , the cleaning module, the printing module 4 , the sample dropping module 5 , the pushing The slide module 6 and the inversion module 64 are arranged along the first working line 101, and the inversion module 64 is located at the intersection of the first working line 101 and the second working line 102; the slide basket drying module 8, dyeing The modules 9 are arranged along the second working line 102, and the first working line 101 and the second working line 102 are vertically intersected or arranged in a straight line or intersected at other suitable angles.

Specifically, as shown in FIGS. 1 to 11 , the first working line 101 is provided with a first linear slide rail 111 , and a slide cart for carrying glass slides is slidably connected to the first linear slide rail 111 . ; The second working line 102 is provided with a second linear slide rail 112, and the second linear slide rail 112 is slidably connected with a manipulator module 113 for clamping and moving glass slides; the first working line 101 is connected to the The second working lines 102 are vertically intersected or arranged in a straight line. For example, in this embodiment, the first working line 101 and the second working line 102 are arranged in a "T" shape, and the inversion module 64 is located at the intersection of the first working line 101 and the second working line 102 , wherein The slide basket drying module 8 and the staining module 9 are located on the left and right sides of the flipping module 64 respectively, and the slide loading module 3, cleaning module, printing module 4, sample dropping module 5, and slide pushing module 6 are located on the same side of the flipping module 64. .

Specifically, as shown in FIG. 1 to FIG. 11 , the smear preparation device further includes a sample automatic sampling module 114 , the sample automatic sampling module 114 is disposed adjacent to the sampling module 1 , and the sample automatic sampling module 114 can send the test tube rack to the sampling module 1; the sample automatic sampling module 114 includes a test tube rack feeding part and a sample mixing part.

Specifically, as shown in FIGS. 1 to 11 , the slide loading module 3 serves as a slide storage and distribution module, which includes a slide storage sub-module 31 for accommodating slides, The slide storage sub-module 31 is pushed to the cutter head sub-module 33 of the working line, and the in-position blocking sub-module 32 for limiting the position of the slide or cutter head sub-module 33 .

Specifically, as shown in FIGS. 1 to 11 , the slide pushing module 6 includes a slide pushing blade sub-module 61 for smoothing the sample, and an inversion module 64 for turning the slide from flat to upright.

Specifically, as shown in FIG. 1 to FIG. 11 , the smear preparation device has a control part for controlling the manipulator to grip and move the slides manually inserted into the slide basket drying module 8 to the staining module 9 . When it is only necessary to stain the glass slides that have dripped blood samples, the glass slides that have dripped blood samples can be directly placed at the slide basket drying module 8, and the control part can make the manipulator clamp at the slide basket drying module 8. The slide is taken and sent to staining module 9 . The control member may be a button or the like. The slide basket drying module 8 has a first accommodating groove 81 and a second accommodating groove 82 for accommodating the slides coated with the sample, and drying airflow can flow out from the inside of the first accommodating groove 81 and the second accommodating groove 82 to dry the sample . The second accommodating groove 82 can be used for placing the manually inserted glass slides. Through the control component, the robotic arm module 113 can clamp the glass slides in the second accommodating groove 82 and place them into the staining station. The first accommodating groove 81 and the second accommodating groove 82 correspond to the first drying station and the second drying station, respectively.

Specifically, as shown in FIGS. 1 to 11 , the staining module 9 includes a turntable sub-module, a needle-disc sub-module and a staining box; the staining module 9 has a slide entry station 901 (staining station 1) and a slide Take out station 902 (staining station 4), the slide entry station 901, the slide take-out station 902, the inversion module 64, the slide basket drying module 8, and the slide output module 10 are arranged along the same straight line, so that , a manipulator that can move linearly can move the slide to each corresponding station.

Specifically, as shown in FIG. 1 to FIG. 11 , the slide basket drying module 12 includes a housing 120, and the housing 120 is provided with a blowing port 1201 for blowing out airflow and an air suction port 1202 for sucking airflow, so that , a better convection effect can be formed, which is beneficial to the rapid drying of the slides in the slide basket 11 . The housing 120 may have two air flow channels, namely, an air outlet channel and an air intake channel. An air outlet fan may be provided at the air outlet channel, and an air intake fan may be provided at the air intake channel. Alternatively, there may be only one circulation channel in the housing 120, and the two ends of the circulation channel are the air outlet 1201 and the air suction port 1202 respectively.

In this embodiment, as shown in Fig. 1 to Fig. 11 , all the working positions can be designed as two working lines intersecting vertically, including: the first working line 101 from the right side to the left side of the smear preparation device (the pusher working line), the second working line 102 (dyeing and output working line) perpendicularly intersecting with the pusher working line. There are loading, printing, blood dripping, pushing and flipping work stations from left to right on the slide pushing work line, and slide output, blood film drying, and blood film unfolding detection are distributed from front to back on the staining and output work line. , Turn over, staining station 1 (slides in), staining station 4 (slides out). The two station lines intersect at the inversion station, and the slide placement state (from horizontal to vertical) is switched in the inversion station. This layout can ensure that the whole machine can always keep the slides in an accurate position control state under the premise that the whole machine can meet the realization of various functions, and minimize the number of clamping times of the slides, which solves the problem of the slides during the operation of the instrument. card failure. At the same time, because the workstations are designed on two concentrated workstation lines, it also plays an important role in reducing the volume of the whole machine.

In the station design of the working line, considering that the printing is easily affected by the quality of the external input slides, and there are many failures, the blood dripping station design of the sample is arranged after the printing station. This solution can effectively avoid the need to re-collect the sample and drip blood due to the failure of the sample due to a small fault in printing, especially for a small amount of samples, such a design is necessary. At the same time, in order to reduce the influence of free foreign matter on the glass slide surface on the printing quality, a special cleaning station is designed between the slide storage and distribution module and the slide information printing module 4 . A special cleaning module is used to clean the free foreign objects on the surface of the glass slides, so that when the glass slides are printed, there are no free foreign objects on the surface of the printing area, and the failure of printing due to the influence of the surface quality of the glass slides is minimized.

In this embodiment, the automatic sample injection module 114 is mainly composed of an injection sub-module and a sampling sub-module, and can automatically perform sample collection and processing on the closed test tubes continuously or discontinuously arranged on the test tube rack. The structure of the sample injection module and the sampling sub-module is relatively independent, and can be used in different application environments (such as using an automatic blood smear preparation device alone or using a fully automatic blood smear preparation device in a fully automatic assembly line). It is very convenient to install and remove, but it does not affect the functional integrity of the sampling sub-module. The automatic blood smear preparation instrument can be quickly configured to different scenarios, such as independent or high-speed, automatic re-examination functions.

The micro-injection module 2 can also be used as an open injection module. When the sample volume is relatively small, or after the pusher is started, it is necessary to queue up to process a certain sample first, then the micro-injection module 2 can be used. The micro injection module 2 can automatically collect and process a single sample put into the test tube adapter. It may include sample feeding, sample unloading functions. In the micro-injection module 2, the sampling needle used for sample collection and the blood dropping needle 501 used in the sample dropping module 5 use the same needle, which can minimize the requirement for sample volume; at the same time, because the same needle is used, the sample The transportation distance is short, which can effectively reduce the deformation effect of the sample on the visible cells of the sample during transportation in the instrument.

As shown in Figures 1 to 4, the slide loading module 3 (slide storage and distribution module) is mainly composed of a slide box module 31, a cutter head sub-module 33, and an in-position blocking sub-module 32. The stacked glass slides in a horizontal state are automatically loaded into the slide slide cart of the slide slide module 6 one by one by the slide storage and distribution module as required by the sample. The slide box can be designed with a relatively independent structure, but it is different from the easily disassembled structure. Under normal working conditions, the slide box is locked by a special locking mechanism. By locking, the relative positional accuracy of the slide cassette and the slide distribution guide rail can be reliably ensured, and the slide cassette with an easily disassembled structure can be prevented from shaking due to external interference, resulting in failure of slide distribution. In the process of troubleshooting and instrument maintenance, the operator can easily remove the slide box by opening the special locking mechanism to achieve the purpose of independent disassembly.

The printing module 4 can use the thermal transfer method to print the information preset by the user (may be serial number, time, type, etc.) on the frosted area of the glass slide to identify the glass slide. The ribbon and the slide can be static and the print head moves relative to the ribbon to achieve absolute stillness of the ribbon and the slide, which can avoid the breakage of the ribbon due to the movement of the ribbon and the slide during printing to the greatest extent possible.

The sample dropping module 5 is mainly divided into a sample suction sub-module and a blood dropping sub-module. The blood-dropping needle 501 adopts a guide sheath with an elastic structure. When the blood drop needle 501 moves downward and touches the glass slide, when the blood drop needle 501 is restricted from moving downward and stops, because of the elastic structural design of the guide sleeve, the fixed bracket of the blood drop needle 501 can continue to move downward. A small buffer distance. In this way, when the blood dropping needle 501 drips blood, the bottom end surface of the blood dropping needle 501 can always be in contact with the glass slide surface without very precise position control, so as to realize the consistency of the shape of the blood drop on the glass slide surface.

As shown in FIG. 1 to FIG. 11 , the blade pushing module 6 is mainly divided into a blade pushing cart sub-module 62 , a blade pushing cutter head sub-module 61 , a blade cleaning sub-module 63 , and a turning module 64 . Including: slide clamping, slide parameter control, slide unloading, slide flipping, and blade cleaning functions. The slide-pulling cart sub-module 62 utilizes the power of its own motion to realize the fixing and loosening of the slide on the cart. And through one clamping, the five actions of loading, printing, dripping blood, pushing and flipping the slide are completed, which can effectively prevent the slide from being damaged and reduce the failure during the transportation of the slide. The flipping module 64 adopts a single-piece flipping structure, which realizes simple structure and reliable flipping. During the flipping process, the glass slide is flipped at a small angle first, and then flipped to the vertical position after waiting for the preset time of the system. This can effectively solve the problem of bleeding at the place where the blood film drips after the film is pushed because the blood film is too thick.

As shown in FIGS. 1 to 11 , the manipulator module 113 is used as the main carrying mechanism of the dyeing and output work line, and realizes the slide output, blood film drying, blood film unfolding detection, flipping, dyeing process when the slide is placed vertically. Transfer between position 1 (slides in), staining station 4 (slides out). Slide output station, slide drying station (blood film drying station), slide pushing station (blood film development detection station), turning station, dyeing station 4, dyeing station 1 these six stations The linear distribution design enables the manipulator to simply complete all station actions in the linear direction. The slide basket drying module 8 can also be called a blood film drying module, to realize the function of drying the blood film of the slides completed by pushing the slides. When the user needs to perform single staining, manually put the slide completed by pushing the slide into the blood film drying module. The slide basket drying module 8 not only serves as a blood film drying module in the whole machine structure, but also provides an interface for the unstained glass slides input from the outside. On the premise of not reducing the function of the instrument, the structure of the whole machine is simplified and the whole machine is realized. streamline. At the same time, the module controls the relative humidity of the drying medium, the drying temperature, and the flow rate of the drying medium through design, which greatly improves the drying speed. In case of the problem that the blood film cannot be completely dried. In addition, this module proposes an air blow drying method in which the drying airflow flows along the direction of the sample (blood film), which can ensure that the cell shape of the blood film does not change under the drying of the micro airflow, and solves the problem of other products in the air drying process. The resulting cell deformation gives wrong diagnostic conclusions.

The dyeing module 9 dyes the slides completed by pushing the slides according to the dyeing process pre-selected by the user. Mainly divided into: turntable sub-module, needle plate sub-module and blocking sub-module 32, dyeing box. The turntable sub-module, together with the staining box, provides the required place for isolating the stained slides and completes the staining process. The needle plate sub-module carries all the adding and discharging needles, and all the needles move up and down with the needle plate, and are inserted into or lifted out of the dyeing box together to realize the addition and discharge of the dye solution. In the dyeing module 9, there are at least one or more blocking sub-modules 32, and the blocking sub-modules 32 realize the switching of the dyeing box from the previous dyeing station to the next dyeing station. The dyeing module 9 adopts a disc structure in structure, which realizes a compact structure. The needle plate is used together with all the liquid addition and discharge needles to go up and down as a whole, which avoids the problem that each dyeing station needs a set of power mechanisms for the liquid addition and liquid discharge needles. The dyeing box uses the friction between the dyeing box and the turntable on the turntable to transfer together with the turntable. The blocking mechanism is used to control the time point of the dyeing box transfer, which realizes the user-defined dyeing process.

As shown in FIGS. 1 to 11 , the slide output module 10 is mainly composed of a slide basket loading sub-module 115 , a slide basket feeding sub-module 116 , a slide drying sub-module (the slide basket drying module 12 ) and the slides Basket unloading sub-module 117, including loading, slide basket transport, slide storage, and slide basket drying functions. The slide basket drying module 12 adopts the method of blowing hot air to dry the slides, relies on heating to evaporate the water on the surface of the slides, and takes away the moisture through the flowing air, so as to achieve the drying effect. The area of the air outlet 1201 is large, and multiple glass slides can be placed at one time, thereby improving work efficiency. Meanwhile, the slide basket drying module 12 is designed with an air outlet 1201 and an air suction outlet 1202 , so that the passage of the slide basket is located between the air passage of the air outlet 1201 and the air inlet 1202 . When the slide basket loaded with slides arrives at the designated drying position, the slide basket drying module 12 blows out strong wind of constant temperature and constant speed from the air outlet 1201 , and at the same time, the starting flow rate from the air suction port 1202 is much larger than that of the air outlet 1201 . The fan draws in air, creating effective convection at the slide basket. At the same time, with the hollow design around the glass slide basket, the drying time is greatly reduced under the double influence of temperature and air convection.

As shown in FIG. 1 and FIG. 10 , the present embodiment further provides a slide basket drying module 12, which includes a casing 120, and the casing 120 is provided with a blowing port 1201 for blowing out airflow and a suction port for inhaling airflow. The tuyere 1202. In this way, a better convection effect can be formed, which facilitates rapid drying of the slides in the slide basket. The air outlet 1201 may face the top of the slide basket, and the air suction port 1202 may face the side wall of the slide basket. The housing 120 may have two air flow channels, namely, an air outlet channel and an air intake channel. At least one air outlet fan may be provided at the air outlet channel, and at least one air intake fan may be provided at the air intake channel. Alternatively, there may be only one circulation channel in the housing 120, and the two ends of the circulation channel are the air outlet 1201 and the air suction port 1202 respectively. The slide basket drying module 12 can make the slide basket channel right between the air duct of the air outlet 1201 and the air suction port 1202 . When the slide basket loaded with slides arrives at the designated drying station, the slide basket drying module 12 blows out strong wind of constant temperature and constant speed from the air outlet 1201 , and at the same time, the starting flow rate from the air suction port 1202 is much larger than the flow rate of the air outlet 1201 The fan sucks the air and forms an effective convection at the slide basket. At the same time, with the hollow design around the glass slide basket, the drying time is greatly reduced under the double influence of temperature and air convection.

As shown in FIGS. 1 to 11 , an embodiment of the present invention also provides a method for preparing a smear, which can be applied to the above-mentioned device for preparing a smear. The method for preparing a smear includes the following steps: a sampling module 1 , a glass slide are arranged and arranged The working lines of loading module 3, printing module 4, sample dropping module 5, slide pushing module 6, flipping module 64 and staining module 9, lay the slide flat and move the slide along the working line. During the movement of the working line, the glass slide is cleaned by the cleaning module, then the sample information is printed on the glass slide by the printing module 4, and then the sample collected from the sampling module 1 is dropped on the glass slide by the sample dropping module 5. , and then use the slide pushing module 6 to smooth the sample of the glass slide, and then use the inversion module 64 to turn the glass slide from a flat state to an upright state, and use the staining module 9 to dye the flipped glass slide.

Specifically, in the process of inverting the glass slide from the flat state to the upright state, the flipping module 64 flips the glass slide to tilt and stays for a set period of time, so that the sample is dried to a certain degree and then turns to the upright state, so as to ensure Diagnostic accuracy.

Specifically, after the sample is printed on the glass slide and before the sample is dropped on the glass slide, the sample information printed on the glass slide is checked by the detection module.

In a specific application, the front end of the smear preparation device can be the sample automatic sampling module 114, where the user puts the sample to be tested, or the sample is automatically transferred in by other workstation devices (eg, a cascade assembly line). In this module, functions including test tube injection, test tube presence or absence detection, barcode scanning, sample mixing, and puncture suction are completed. For special samples (such as body fluids, etc.) or open-capped samples, the micro/open sampling module can be used to aspirate the sample, which can ensure that slide staining can also be performed in the case of a relatively small sample volume. At the same time, the sample automatic sampling module 114 and the micro/open sampling module respectively transmit the collected sample information to the drop sampling module 5 for processing, so as to extract the sample characteristics related to the slides for subsequent slides.

The right side of the smear preparation device can be the slide storage and distribution module, where the user loads stacks of horizontal slides, and the slide storage and distribution module automatically loads the slides one by one according to the sample requirements. The pusher trolley of the pusher module 6 is fixed on the trolley in a flat state. In the slide storage and distribution module, a special slide cleaning station is designed, and a special cleaning module is used to clean the free foreign matter on the surface of the slide, so that when the slide is printed, there is no free foreign matter on the surface of the printing area. Minimize print failures that are affected by the quality of the slide surface. Afterwards, the slides are gradually moved to the left side of the smear preparation device on the slide slide carriage of the slide slide slide module 6, together with the slide carriage. In this embodiment, the trolley first arrives at the printing position, and cooperates with the slide information printing module 4 to complete the printing of the sample information; after confirming that the printing action is successfully completed, it arrives at the blood dripping station; cooperates with the dripping module 5 to automatically inject the sample into the sample module 114 or the sample collected by the micro/open injection module is dropped onto the glass slide; when it reaches the slide position, such as the characteristics of the sample extracted by the drop sample module 5, the slide slide parameters are adjusted intelligently, so that the sample is smeared on the glass slide in the best state. Then, the slide is finally flipped from the horizontal state to the vertical state by the flipping module 64 of the slide-pushing module 6 .

The manipulator module 113 on the left side of the smear preparation device runs through the front and rear directions of the entire instrument, connecting the flip position, blood film drying position, staining station 1, staining station 4, and slide output position of the slide in a vertical state. The manipulator module 113 transfers the finished slide slide to the blood film drying module at the flip position of the slide pushing module 6, and the sample is fully dried in the blood film drying module to ensure that the blood film will not fall off in the subsequent staining process. After a fixed period of time, the manipulator module 113 transfers the glass slides after drying of the samples into the staining box in the station 1 of the staining module 9, and each glass slide corresponds to a staining box. In the staining box, the slides are stained according to the staining process set by the user (the staining process can be customized by the user within a certain range). . The stained slides together with the staining cassette are transferred from staining station 1 to staining station 4 in staining module 9 . The robotic arm module 113 takes out the dyed slides from the dyeing box from the dyeing station 4, and the dyeing box automatically enters the next dyeing cycle.

The manipulator module 113 transfers the slides from the dyeing station 4 of the dyeing module 9 to the slide basket 11 which is sealed at the bottom and hollowed out around the slide output module 10, and is arranged one by one with gaps. The slide output module 10 counts the slides that have been loaded into the slide basket. When the slide basket 11 is full or all the slides are pushed and dyed, the slide output module 10 transports the slide basket 11 to the slide output. At the drying mechanism of the slide basket drying module 12 of the module 10, the drying mechanism blows a strong wind of constant temperature and constant speed to dry the stained slides. After a fixed period of strong air drying, all slides were fully dried. The slide output module 10 pushes the slides together with the slide basket 11 to the unloading stage of the slide output module 10, and at this time, the whole process of pushing the slides and staining is completed.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements or improvements made within the spirit and principles of the present invention, such as Adjusting the order of bits, or merging or decomposing the number of stations, and adjusting the process accordingly should be included within the protection scope of the present invention.

Claims (16)

1. A smear preparation device, comprising: a slide loading module, a slide cart and a slide processing module; the slide loading module is used for loading the slides to be processed on the slide cart The slide cart is used to transport the slides to the slide processing module; the slide processing module includes a printing module, a sample drop module and a slide push module, and the slide cart transports the slides The slides are transported from the slide loading module to the printing module, the sample drop module and the slide push module respectively, and the printing module is used to print information on the slide, and the drop sample The module is used to perform a drop sample operation on the glass slide, and the slide slide module is used to perform sample smearing on the glass slide. 2 . The smear preparation device according to claim 1 , wherein when loading the glass slides, the slide cart clamps the glass slides loaded thereon to complete the fixing of the glass slides; 2 . Before flipping the slides, the slide cart releases the slides thereon for flipping. 3. The smear preparation device according to claim 1 or 2, characterized in that, the smear preparation device further comprises a cleaning module, and the cleaning module is used for cleaning free foreign matter on the surface of the glass slide, The free foreign matter on the surface of the glass slide is caused by friction when the adjacent glass slides slide relative to each other when the glass slide loading module loads the glass slide; the glass slide loading module loads the glass slide to the Film cart. 4 . The smear preparation device according to claim 3 , wherein the slide cart transports the slides from the cleaning module to the printing module along a first direction, and the printing module for printing information on the glass slide after the cleaning module cleans the surface of the glass slide. 5. The smear preparation device according to any one of claims 1 to 4, wherein the smear preparation device further comprises a detection module, and the detection module checks the sample information printed on the glass slide to confirm After the sample information is successfully printed, the slide cart transports the fixed glass slides to the sample dropping module to perform drop sample processing on the glass slides fixed on the slide slide cart. 6. The smear preparation device according to any one of claims 1 to 5, wherein the slide processing module further comprises an inversion module, and the slide cart moves the slide from the slide push module It is transported to the inversion module in a linear direction, and the inversion module is used to invert the glass slide. 7 . The smear preparation device according to claim 6 , wherein the smear preparation device further comprises a slide rail, on which the slide rail is slidably connected with the smear pusher, and the smear pusher is in the The slides are transported to the printing module, the sample dropping module, the slide pushing module and the turning module respectively by sliding on the sliding rail. 8. The smear preparation device according to any one of claims 6 to 7, characterized in that, the smear preparation device further comprises a staining module, a slide basket drying module and a robotic arm module, wherein the robotic arm module The glass slides are transported from the inversion module to the staining module, where the staining module is used to stain the glass slides, and the robotic arm module moves the stained glass slides to a glass slide basket at a predetermined position, The slides together with the slide basket are also transported to the slide basket drying module for drying the stained slides in the slide basket. 9. The smear preparation device according to any one of claims 6 to 7, wherein the smear preparation device further comprises a staining module, a manipulator module and a slide output module, and the manipulator module converts the glass slide The slides are transported from the inversion module to the staining module for staining. The slide output module includes a slide basket loading submodule, a slide basket feeding submodule, and a slide basket unloading submodule. The slide basket loading sub-module is used to load empty slide baskets, the slide basket feeding sub-module is used to transport the empty slide baskets to a predetermined position, and the manipulator module moves the stained slides to the predetermined position In the slide basket, the slide basket unloading sub-module is used to transport the slide basket loaded with slides to an unloading station for unloading. 10. A smear preparation method, characterized in that, comprising: Fix the slides to be processed on the slide cart in a horizontal state; The slides are transported to the printing position, the blood dripping position and the pushing position respectively by the slide cart, so as to print information on the slides on the slide cart at the printing position, and push the slides on the blood dripping station. The glass slides on the slide car are subjected to a sample dropping operation, and the samples on the slide slide car are smeared at the slide push position. 11. The method for preparing a smear according to claim 10, wherein the glass slide to be processed is fixed on the slide cart in a horizontal state, comprising: Before the slide cart transports the slides, the slide cart loads the slides thereon and clamps them; After performing sample smoothing on the slides on the slide cart at the slide slide position, the method further includes: transporting the slide cart to the flip position, which releases the slides thereon; Invert the slides on the slide cart to be vertical. 12. The method for preparing a smear according to any one of claims 10 to 11, wherein the information printing on the glass slide on the printing position comprises: Information is printed on the slide after surface cleaning of the slide. 13. The method for preparing a smear according to any one of claims 10 to 12, characterized in that, performing a dripping operation on the glass slide on the blood dripping station, comprising: After the sample information is printed on the slides, drop samples are processed on the slides fixed on the slide cart. 14. The method for preparing a smear according to claim 13, characterized in that, after the sample information is printed on the glass slide, a drop sample processing is performed on the glass slide fixed on the slide cart, comprising: The sample information printed on the glass slide is checked, and after it is confirmed that the sample information is successfully printed, the sample drop operation is performed on the glass slide. 15. The smear preparation method according to any one of claims 10 to 14, wherein the smear preparation method further comprises: One slide at a time is transported from the push position to the flip position by the slide cart to flip the slide on the flip position. 16. The smear preparation method according to claim 15, wherein the smear preparation method further comprises: When turning over the one glass slide, during the process of turning the one glass slide from the horizontal state to the vertical state, the one glass slide is turned over to the inclined state and stays in the inclined state for a preset period of time.

Images