CN110692850A - Application of clostridium ethanolate protein in poultry feed - Google Patents
Application of clostridium ethanolate protein in poultry feed Download PDFInfo
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 71
- 241000193403 Clostridium Species 0.000 title claims abstract description 67
- HHFAWKCIHAUFRX-UHFFFAOYSA-N ethoxide Chemical compound CC[O-] HHFAWKCIHAUFRX-UHFFFAOYSA-N 0.000 title claims abstract description 52
- 244000144977 poultry Species 0.000 title claims abstract description 40
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- 239000004455 soybean meal Substances 0.000 claims abstract description 11
- 238000011282 treatment Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 241001656809 Clostridium autoethanogenum Species 0.000 claims abstract description 7
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- 239000000203 mixture Substances 0.000 claims description 10
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 9
- 235000019743 Choline chloride Nutrition 0.000 claims description 9
- 239000004470 DL Methionine Substances 0.000 claims description 9
- 240000008042 Zea mays Species 0.000 claims description 9
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- 229910052791 calcium Inorganic materials 0.000 claims description 9
- 229960003178 choline chloride Drugs 0.000 claims description 9
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 9
- 235000005822 corn Nutrition 0.000 claims description 9
- 235000013312 flour Nutrition 0.000 claims description 9
- FFEARJCKVFRZRR-UHFFFAOYSA-N methionine Chemical compound CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 claims description 9
- 235000006109 methionine Nutrition 0.000 claims description 9
- 229930182817 methionine Natural products 0.000 claims description 9
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- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Birds (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to an application of clostridium ethanolate protein in poultry feed, belonging to the field of feed, and the application of the clostridium ethanolate protein in the poultry feed provided by the invention comprises the following steps: performing liquid fermentation culture by using Clostridium ethanolicum (Clostridium autoethanogenum) as a fermentation strain and CO-containing gas as a raw material, centrifuging thallus in the fermentation liquor of the liquid fermentation, and performing primary drying treatment to obtain Clostridium ethanolicum protein: the application of the clostridium ethanolate protein in the poultry feed provided by the embodiment of the invention can obviously improve the growth performance of poultry and obtain higher feed conversion ratio; because the using amount of the soybean meal is reduced, the production cost is reduced, and meanwhile, the dependence degree on foreign imported soybeans is reduced, the clostridium ethanolate protein in the invention patent can be used as a substitute of soybean meal type protein raw materials in poultry feed.
Description
Technical Field
The invention relates to a microbial single cell protein, in particular to an application of clostridium ethanolate protein in poultry feed.
Background
As the first breeding country in the world, the grain concentrated feed accounts for more than 50 parts of grain resources every year, the demand of the feed resources is continuously increased along with the continuous development of animal husbandry, particularly, the protein resources which can be used as the feed are very lack, the price is continuously increased, the dependence of protein raw materials such as bean pulp, fish meal and the like on foreign imports is more than 80 parts, and the gap is continuously enlarged, so that the 'food competition between people and livestock' becomes one of the most main factors influencing the food safety in China. The searching of protein feed resources which can be developed and utilized becomes the key point of future breeding production. The clostridium ethanolate protein is a single-cell protein, the protein content of the clostridium ethanolate protein reaches more than 80 parts, the amino acid is balanced, the clostridium ethanolate protein is rich in various micronutrients, and the clostridium ethanolate protein basically does not contain anti-nutritional factors and animal-derived components.
In the poultry breeding industry, the broiler chickens have the characteristics of high growth speed, strong disease resistance, early marketing time and the like, and representative broiler chicken variety Aijia (AA) broiler chickens are selected as research objects. The feed is required to have higher nutrient concentration and protein energy ratio so as to maintain the growth and development requirements of the broiler chickens; in the broiler breeding process, feeds with different formulas are fed according to different growth stages of broilers respectively so as to meet the growth and development requirements of the broilers, and an effective adding mode and a proper adding amount are provided for a novel single-cell protein raw material namely clostridium ethanolate protein in the feed.
Disclosure of Invention
In view of the above problems, the present invention has been developed to provide a use of clostridium autoethanolate protein in poultry feed that overcomes, or at least partially solves, the above problems.
The embodiment of the invention provides an application of clostridium ethanolate protein in poultry feed, wherein the preparation method of the clostridium ethanolate protein comprises the following steps:
clostridium ethanolicum (Clostridium autoethanogenum) is used as a fermentation strain, CO-containing gas is used as a raw material, liquid fermentation culture is carried out, and thalli in fermentation liquor of the liquid fermentation are subjected to centrifugation and primary drying treatment to obtain Clostridium ethanolicum protein.
Further, the Clostridium autoethanolicum (Clostridium autoethanogenum, deposit No. DSM10061, deposit No. german collection of strains, DSMZ.
Further, the nutritional composition of the clostridium ethanolate protein comprises the following components in parts by weight: more than or equal to 80 parts of crude protein, less than or equal to 7 parts of crude ash, less than or equal to 12 parts of water and less than or equal to 1 part of ammonium salt.
Further, in the application, the preparation method of the poultry feed comprises the following steps: and uniformly mixing the clostridium ethanolate protein, the mixture and water to obtain mixed slurry, preparing the mixed slurry into granules, and performing secondary drying treatment to obtain the poultry feed.
Further, the mixture comprises at least one of the following: corn flour, bean pulp, soybean oil, calcium hydrophosphate, stone powder, choline chloride, premix and DL-methionine.
Further, the particle size of the particles is 2-4 mm.
Further, the drying of the pelleted feed does not result in a poultry feed moisture of greater than 10%.
Further, the poultry feed comprises the following components in parts by weight: 2-4 parts of clostridium ethanolate protein, 47.21-61.22 parts of corn flour, 26.1-42.78 parts of soybean meal, 4.2-6.1 parts of soybean oil, 1.36-1.43 parts of calcium hydrophosphate, 1.08-1.28 parts of stone powder, 0.2 part of choline chloride, 1 part of premix and 0.10-0.16 part of DL-methionine.
One or more technical solutions in the embodiments of the present invention have at least the following technical effects or advantages:
1. the clostridium ethanolate protein provided by the embodiment of the invention can be used as a substitute of protein raw materials in poultry feed, and has no obvious adverse effect on growth performance.
Detailed Description
The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.
Throughout the specification, unless otherwise specifically noted, terms used herein should be understood as having meanings as commonly used in the art. Accordingly, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. If there is a conflict, the present specification will control.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention are commercially available or can be prepared by existing methods.
The embodiment provides an application of clostridium ethanolate protein in poultry feed, wherein a preparation method of the clostridium ethanolate protein comprises the following steps:
performing liquid fermentation culture by using clostridium ethanolate (clostridium autoethanogenum) as a fermentation strain and using CO-containing gas as a raw material, and centrifuging and drying thalli in fermentation liquor of the liquid fermentation for the first time to obtain clostridium ethanolate protein;
in this example, clostridium ethanolate (clostridium autoethanogenum) is deposited under DSM10061 and has a deposit address of german collection of strains, DSMZ;
in the liquid fermentation culture, the concentration of clostridium ethanolate is 20-25g/L, and the introduction amount of CO gas is 8000-10000m3/h；
The centrifugal rotating speed is 4000-4500r/min, and the centrifugal feeding flow is 4-6m3H; the cell concentration after centrifugation is 120-150 g/L.
The primary drying temperature is 160-180 ℃ of air inlet temperature, the air outlet temperature is 75-85 ℃, instantaneous drying is carried out, the drying time is short, and the evaporation capacity of the drying tower is 5000 kg/h.
The clostridium ethanolate protein has high crude protein content (more than or equal to 80 percent), good amino acid balance, the proportion of amino acid in the crude protein is up to more than 90 percent, wherein the lysine content is more than or equal to 7.5 percent, the clostridium ethanolate protein is easy to digest and absorb, the clostridium ethanolate protein is used as a substitute of a protein raw material in poultry feed, has good substitution effect on conventional protein raw materials such as bean pulp and the like, can reduce the import of a large amount of raw materials such as the bean pulp and the like, and has positive effect on solving the problem of food competition with people.
In this embodiment, the nutritional composition of the clostridium ethanolate protein comprises, in parts by weight: more than or equal to 80 parts of crude protein, less than or equal to 7 parts of crude ash, less than or equal to 12 parts of water and less than or equal to 1 part of ammonium salt.
In this example, in the application, the preparation method of the poultry feed comprises the following steps: and uniformly mixing the clostridium ethanolate protein, the mixture and water to obtain mixed slurry, preparing the mixed slurry into granules, and performing secondary drying treatment to obtain the poultry feed.
In this embodiment, the mixture includes at least one of the following: corn flour, bean pulp, soybean oil, calcium hydrophosphate, stone powder, choline chloride, premix and DL-methionine.
In this example, the particle size is 2 to 4 mm.
In this embodiment, the pellet feed is dried by natural air drying.
In the embodiment, the poultry feed comprises the following components in parts by weight: 2-4 parts of clostridium ethanolate protein, 47.21-61.22 parts of corn flour, 26.1-42.78 parts of soybean meal, 4.2-6.1 parts of soybean oil, 1.36-1.43 parts of calcium hydrophosphate, 1.08-1.28 parts of stone powder, 0.2 part of choline chloride, 1 part of premix and 0.10-0.16 part of DL-methionine.
Storage conditions of the poultry feed: storing in normal temperature environment.
The use of the clostridium ethanolate proteins of the present application in poultry feed will be described in detail below with reference to specific examples.
Example 1
The poultry feed comprises the following components in parts by weight: 2 parts of clostridium ethanolate protein, 50.34 parts of corn flour, 38.39 parts of soybean meal, 4.94 parts of soybean oil, 1.43 parts of calcium hydrophosphate, 1.24 parts of stone powder, 0.3 part of salt, 0.2 part of choline chloride, 1 part of premix and 0.16 part of DL-methionine.
The preparation method of the poultry feed comprises the following steps: adding clostridium ethanolate protein into the mixture, mixing uniformly, modifying, and making into granules with the particle size of 2.0-4.0mm by using an F-26(II) type granulator to obtain the poultry feed.
Example 2
This example differs from example 1 in that:
the poultry feed comprises the following components in parts by weight: 4 parts of clostridium ethanolate protein, 53.44 parts of corn flour, 34.01 parts of soybean meal, 4.2 parts of soybean oil, 1.42 parts of calcium hydrophosphate, 1.28 parts of stone powder, 0.3 part of salt, 0.2 part of choline chloride, 1 part of premix and 0.15 part of DL-methionine.
Comparative example 1
No Clostridium ethanolicum protein was added to this comparative example.
The feed formula comprises the following components in parts by weight: 47.21 parts of corn flour, 42.78 parts of soybean meal, 0 part of clostridium ethanolate protein, 5.71 parts of soybean oil, 1.43 parts of calcium hydrophosphate, 1.22 parts of stone powder, 0.3 part of salt, 0.2 part of choline chloride, 1 part of premix and 0.15 part of DL-methionine.
Mixing the above feed formulas, adjusting quality, and granulating with F-26(II) type granulator to obtain granule with particle size of 2.0-4.0 mm.
Experimental example 1
1. Materials and methods
The test was divided into 3 treatment groups, D1 (basal control group ration), D2 (basal formula +2 parts clostridium ethanolate protein), D3 (basal formula +4 parts clostridium ethanolate protein).
The breeding test was conducted in a test base at the south of the academy of agricultural sciences of China, and 324 representative broiler chicks (half of the male and the female) with Aijia meat were selected from poultry, purchased from Beijing Huadu broiler company. The broiler chicken nutritional requirement standard is prepared according to American NRC (1994), and adopts corn-soybean meal type basic ration. The positive control group D1 was fed a basal diet and the test groups (D2 and D3) were fed a diet supplemented with 2 and 4 parts of clostridia protein powder to the basal diet. Adopts double-layer cage culture, and can feed and drink water freely. Temperature in the chicken house: 33-34 ℃ after 1-3 days of age, reducing the temperature by 3 ℃ every week to 23-25 ℃ and maintaining the temperature within the temperature range, and timely adjusting the temperature according to the state of the chickens. The whole period (1-42 days old) is 24 h. Cleaning dung once every 5 days in the early stage of feeding, cleaning dung once every 3-4 days in the later stage, and carrying out spray disinfection once for cleaning dung for chicken each time. At the age of 7 days, carrying out nasal drip and eye drop immunization on the newcastle disease and infectious bronchitis combined live vaccine; at the age of 14 days, the vaccine for infectious bursal disease is used for drinking water immunization. Animals were observed daily for health including mental status, appetite and fecal status and the number of deaths recorded.
2. Main measuring index and statistical method
The growth performance indexes including body weight, daily feed intake, feed-meat ratio and death rate are mainly measured. The test data are statistically analyzed by one way ANOVA in SAS8.0, the mean value comparison among the treatments is subjected to significance test by Duncan's multiple comparison, the result is expressed by the mean value +/-standard deviation, and P < 0.05 is used as a significance judgment standard.
3. Results and analysis
3.1 Effect of Clostridium ethanolicum proteins on broiler feed intake
As can be seen from Table 1, the differences in daily food intake of the treatment groups at 1-22 days old, 22-24 days old and 1-42 days old were not significant (P > 0.05), indicating that the addition of Clostridium ethanolate protein in the daily ration did not adversely affect the food intake.
3.2 Effect of Clostridium ethanolicum proteins on broiler weight and daily gain
As can be seen from Table 1, the initial weight difference of the broilers between treatments is not significant (P > 0.05), the daily weight difference of treatment groups at 22-42 days of age is not significant (P > 0.05), the daily weight gains of the broilers at 1-21 days of age are significantly higher than D1(P < 0.05), wherein the difference between D2 and D3 is not significant (P > 0.05); the daily gain of the 1-42 days of age is significantly higher in the D2 and D3 groups than in the D1 group, and the difference between the D2 and D3 is not significant. The result shows that the weight gain effect of the group which adds 2 parts of clostridium autoethanolicum protein powder in the daily ration is optimal.
3.3 Effect of Clostridium ethanolicum proteins on feed conversion ratio of broiler chickens
As can be seen from Table 1, the F/G difference between the treatment groups at 1-21 days of age, 22-42 days of age and 1-42 days of age was significant (P < 0.05), with the broiler chickens at 1-21 days of age F/G, the D2 and D3 groups being significantly lower than the D1 group (P < 0.05), with no significant difference between the D2 and D3 groups (P > 0.05); broiler chickens F/G, D2 and D3 at 22-42 days of age and 1-42 days of age were significantly lower than D1(P < 0.05), with no significant difference between D2 and D3 (P > 0.05). The results show that the addition of 2 parts of clostridium ethanolate protein in the daily ration can effectively reduce the addition of soybean meal in the daily ration, and has no obvious adverse effect on the feed conversion ratio.
TABLE 1 Effect of different levels of Clostridium ethanolicum protein in diets on broiler growth Performance
In the research of the invention, the clostridium ethanolate protein in the daily ration replaces the soybean meal in the daily ration of the broiler chicken, so that the daily feed intake of the broiler chicken is not obviously influenced; compared with a control group, the daily gain of the broiler chickens of the clostridium ethanolate protein groups of 2 parts and 4 parts is obviously increased, and the feed-meat ratio is obviously reduced. Therefore, the clostridium ethanolate protein can be used for replacing conventional raw materials such as soybean meal and the like in the poultry feed, and 2 parts of clostridium ethanolate protein is most suitable for being added into the broiler feed in consideration of comprehensive cost factors.
Finally, it should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (8)
1. The application of clostridium ethanolate protein in poultry feed is characterized in that the preparation method of the clostridium ethanolate protein comprises the following steps:
clostridium ethanolicum (Clostridium autoethanogenum) is used as a fermentation strain, CO-containing gas is used as a raw material, liquid fermentation culture is carried out, and thalli in fermentation liquor of the liquid fermentation are subjected to centrifugation and primary drying treatment to obtain Clostridium ethanolicum protein.
2. The use of a Clostridium ethanolate protein in poultry feed according to claim 1, wherein Clostridium ethanolate (Clostridium autoethanogenum) is deposited under DSM10061 with the deposit address of the german collection of cultures, DSMZ.
3. The use of clostridium autoethanolate protein in a poultry feed according to claim 1, wherein the nutritional composition of the clostridium autoethanolate protein comprises, in parts by weight: more than or equal to 80 parts of crude protein, less than or equal to 7 parts of crude ash, less than or equal to 12 parts of water and less than or equal to 1 part of ammonium salt.
4. The use of clostridium autoethanolate protein in a poultry feed according to claim 1, wherein the poultry feed is prepared by the following method: and uniformly mixing the clostridium ethanolate protein, the mixture and water to obtain a mixed material, preparing the mixed material into granules, and drying to obtain the poultry feed.
5. The use of clostridial ethanolate protein in a feed for birds according to any one of claim 4, wherein the mix includes at least one of: corn flour, bean pulp, soybean oil, calcium hydrophosphate, stone powder, choline chloride, premix and DL-methionine.
6. The use of clostridial ethanolate protein in a poultry feed according to claim 4, wherein the particle size is 2-4 mm.
7. The use of a clostridium autoethanolate protein in a poultry feed according to claim 4, wherein the moisture content of the poultry feed dried after pelleting is not higher than 10%.
8. The use of clostridial ethanolate protein in a poultry feed according to claim 4, wherein the poultry feed comprises, in parts by weight: 2-4 parts of clostridium ethanolate protein, 47.21-61.22 parts of corn flour, 26.1-42.78 parts of soybean meal, 4.2-6.1 parts of soybean oil, 1.36-1.43 parts of calcium hydrophosphate, 1.08-1.28 parts of stone powder, 0.2 part of choline chloride, 1 part of premix and 0.10-0.16 part of DL-methionine.
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| CN201910957660.1A CN110692850A (en) | 2019-10-09 | 2019-10-09 | Application of clostridium ethanolate protein in poultry feed |
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Cited By (5)
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| CN111820323A (en) * | 2020-06-10 | 2020-10-27 | 北京首钢朗泽新能源科技有限公司 | Application of clostridium ethanolate protein as high apparent digestibility protein in feed |
| CN112056466A (en) * | 2020-09-10 | 2020-12-11 | 北京首钢朗泽新能源科技有限公司 | Application of clostridium ethanolate protein in pet feed |
| CN114304392A (en) * | 2021-12-16 | 2022-04-12 | 贵州金泽新能源科技有限公司 | A kind of feed, feed additive and preparation method and system thereof |
| CN117882806A (en) * | 2024-03-05 | 2024-04-16 | 江苏三仪动物营养科技有限公司 | A low-protein diet for broiler chickens and its application |
| CN118044569A (en) * | 2024-03-05 | 2024-05-17 | 江苏三仪动物营养科技有限公司 | A premix for energy saving and emission reduction instead of soybean meal and its application in animal breeding |
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| US20050266050A1 (en) * | 2002-09-18 | 2005-12-01 | Smith Stephen R | Antimicrobial composition and method for use |
| CN106689786A (en) * | 2016-12-21 | 2017-05-24 | 辽宁禾丰牧业股份有限公司 | Compound feed for promoting development of muscular stomach of broiler and preparation method of compound feed |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111820323A (en) * | 2020-06-10 | 2020-10-27 | 北京首钢朗泽新能源科技有限公司 | Application of clostridium ethanolate protein as high apparent digestibility protein in feed |
| CN112056466A (en) * | 2020-09-10 | 2020-12-11 | 北京首钢朗泽新能源科技有限公司 | Application of clostridium ethanolate protein in pet feed |
| CN114304392A (en) * | 2021-12-16 | 2022-04-12 | 贵州金泽新能源科技有限公司 | A kind of feed, feed additive and preparation method and system thereof |
| CN117882806A (en) * | 2024-03-05 | 2024-04-16 | 江苏三仪动物营养科技有限公司 | A low-protein diet for broiler chickens and its application |
| CN118044569A (en) * | 2024-03-05 | 2024-05-17 | 江苏三仪动物营养科技有限公司 | A premix for energy saving and emission reduction instead of soybean meal and its application in animal breeding |
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Application publication date: 20200117 |