CN106860647A - A kind of preparation method and applications of natural source bouvardin - Google Patents
A kind of preparation method and applications of natural source bouvardin Download PDFInfo
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- CN106860647A CN106860647A CN201710250940.XA CN201710250940A CN106860647A CN 106860647 A CN106860647 A CN 106860647A CN 201710250940 A CN201710250940 A CN 201710250940A CN 106860647 A CN106860647 A CN 106860647A
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- bouvardin
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- A—HUMAN NECESSITIES
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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Abstract
The present invention relates to a kind of preparation method and applications of natural source bouvardin, it is prepared from by raw material of hawthorn, red bayberry, wild jujube, orange, walnut, loquat, date, apricot, peach, plum, shaddock, pine nut, the fruit stone of fibert or shell, has a broad antifungal spectrum, inactivating efficacy to virus, fungi, bacterium is notable, safety and stability, with mucous membrane wound healing is promoted, pathological tissues enhancing natural defense function is repaired, can effectively prevent and treat infringement of the pathogenic microorganism to human body.Natural source bouvardin of the present invention can be widely applied to the multiple fields closely related with human habitat such as prevention from suffering from the diseases, anti-ageing health care, skin nursing, amenities, cosmetics, daily chemical product, air sterillization, food, medicine, feed addictive, food additives, deodorant, soil conditioner, the prevention and control of plant diseases, pest control, raising vegetables and crop yield, with extensive value and DEVELOPMENT PROSPECT.
Description
Technical field
The present invention relates to field of antibiotics, and in particular to a kind of preparation method and applications of natural source bouvardin.
Background technology
Antibiotic (antibiotics) is in life by microorganism (including bacterium, fungi, actinomyces) or high animals and plants
It is produced with antipathogen or other active secondary metabolites during work, other living cells can be disturbed to develop work(
The chemical substance of energy.Existing clinical conventional antibiosis have in transgenic engineered bacteria nutrient solution extract and chemically synthesizes
Or semi-synthetic compound.
Current abuse of antibiotics has become a serious social concern.Abuse of antibiotics, can cause flora imbalance.
In the human body of normal human subject, often all contain a certain amount of normal flora, they are the beneficial bacteriums of people's normal activities, ginseng
With the eubolism of the person.Simultaneously in the body of human body, as long as the presence of these profitable strains, other are harmful
Flora is not easy in these place existence.And people, while abuse of antibiotics, antibiotic can not be recognized to the mankind
Beneficial or pernicious bacteria, result is that the normal flora of the person is also killed.So other harmful bacterias will be herein
Breeding, so as to form " superinfection ", this frequently can lead to using other antibiotic ineffectives, and the death rate is very high.Antibiotic
Abuse and misuse, also result in " super infection " that many medicines cannot be treated, and generate " superbacteria " and " super disease
Poison ".
The harm of abuse of antibiotics：
1st, bacterial resistance is induced：Pathogenic microorganism is constantly being made a variation to hide medicine, and antibody-resistant bacterium is also produced therewith.Mesh
Before, almost do not exist resistance phenomenon without a kind of antibacterials.
2nd, human organ is damaged：Antibiotic will also result in human body infringement simultaneously sterilized, influence liver, renal function, stomach and intestine
Road reaction etc..
3rd, suprainfection is caused：Under normal circumstances, the oral cavity of human body, respiratory tract, enteron aisle have bacterium parasitic, post and grow bacterium
Group maintains poised state under mutual antagonism.If long-term use extensive pedigree antibiotic, sensitive bacteria can be killed, and insensitive
Bacterium seizes the opportunity breeding, and not repressed bacterium, fungi and foreign bacteria can also take advantage of a weak point, and induce another infection.
4th, social danger is caused：Abuse of antibiotics may cause the generation of some bacterial resistance phenomenons, the treatment to infecting
Can become very difficult.
Additionally due to long-term abuse of antibiotics, many pathogenic microorganisms very sensitive to antibiotic generate drug resistance,
Magical antibiotic various in the past are made to lose original power.In order to overcome antibiotic shortcoming in itself and avoid because using not
When and the consequence brought, people must not the use of non-confrontational raw element carry out strict limitation, while increasing the new antibiosis of research and development
The dynamics of element, natural source bouvardin is exactly one of antibiotics that this people expect, current each state is all studying and opening
Hair.China is the cradle of herbal medicine, and antibacterial Chinese herbal medicines has once played important function during anti-inflammation, but these
Antibacterial Chinese herbal medicines exploitation established practice modelling, formulation, its technics comparing are complicated, relatively costly, and these antibacterial Chinese herbal medicineses antibacterial
General relative narrower, what is had is only effective to gram-positive bacteria, and what is had is only effective to Gram-negative bacteria, and what is had only has to virus
Effect, before not yet pathogen is determined, it is difficult to accurate medication.
It is free from environmental pollution with to people, animal safety because natural source bouvardin derives from nature, it is difficult to cause anti-
The property of medicine, is easy to the advantages of degrading, therefore research and development safe plant medicine and plant pesticide in natural environment, into
It is the focus of current new type bactericide innovative research.Meanwhile, the bactericide of plant origin is also to excavate a new generation's medicine and agricultural chemicals
One of important channel of primer.
Through retrieval, the features such as there is strongly active and strong penetrability using fruit and nut prepared product, to bacterium, fungi, disease
Poison, black withered grass gemma etc. are respectively provided with obvious killing effect, can be used to prevent and treat cause pathogeny imcrobe infection, while also having regulation
The natural source bouvardin of human immune system's effect has not been reported.
The content of the invention
It is an object of the invention to provide a kind of method that utilization fruit and nut prepare natural source bouvardin.
The technical solution adopted for the present invention to solve the technical problems is：A kind of preparation side of natural source bouvardin
Method, comprises the following steps：
1) by Chinese hawthorn seed, red bayberry nucleocapsid, spina date seed shell, orange core, peach-pit shell, loquat seed, date nucleocapsid, almond shell, peach
Nucleocapsid, plum nucleocapsid, shaddock core, pine nut shell, hazelnut shell are added separately to retort for raw material, using pyrolysis technology, according to
Initial stoste is collected according to the difference of above-mentioned raw material thermal cracking, being divided into three phases is carried out, and is respectively 80~180 DEG C (RF1),
181~380 DEG C (RF2), 381~550 DEG C (RF3) merges the initial stoste that every kind of raw material three phases are collected into respectively
Obtain the initial stoste of merging of every kind of raw material；
2) by step 1) the initial stoste of merging of every kind of raw material that obtains proportionally mixes, and obtains compound initial former
Liquid；
3) by step 2) the compound initial stoste that obtains is stored in storagetank and stands 1-3 month, allows labile element to send out completely
Raw oxidation, polymerization and precipitate, remove suspended material and other solid particles, take middle level supernatant liquid with pumping, add former
Liquid pool, enters fine filtering device through raw water pump again after filtering, then successively through ultrafiltration apparatus, nanofiltration device, high pressure reverse osmosis membrane dress
Put and adjust rate of flow of fluid and operating pressure, adjustment with low pressure reverse osmosis film device, the corresponding concentrated water partial reflux of each device of control
Technological parameter, determines the stoste treating capacity of equipment stable operation for 300L/h, to compound initial stoste low temperature concentration point water, obtains
The transparency liquid of stabilization is natural source bouvardin.
Specifically, the step 2) in every kind of raw material the initial stoste of merging ratio in parts by weight, it is specific as follows：
Chinese hawthorn seed 4-8.5 parts, 4.5-7 parts of red bayberry nucleocapsid, 4-7.5 parts of spina date seed shell, 4-7 parts of orange core, 5-9.5 parts of peach-pit shell, loquat
Core 5-8 parts, 5-5.5 parts of date nucleocapsid, almond shell 5.5-9.5 parts, 6-9 parts of peach-pit shell, 4-6.8 parts of plum nucleocapsid, shaddock core 4-7
Part, 3.5-6 parts, hazelnut shell 3.5-6.5 parts of pine nut shell.
Specifically, the step 3) in obtained natural source bouvardin with phenol and organic acid content meter, wherein phenol
Class is not less than 7.04% in terms of phenol content；Organic acid is not less than 18.61% in terms of acetic acid content, and density is 1.0033；
PH value is 1.5~3.0.
Natural source bouvardin another object of the present invention is to provide preparation is answered as antifungal, bacterial drug
With.
Another object of the present invention is to provide the natural source bouvardin of preparation as the application of antiviral drugs.
Another object of the present invention is to provide application of the natural source bouvardin of preparation in terms of plant growth.
Another object of the present invention is to provide the natural source bouvardin of preparation as the application of feed addictive.
Application of the natural source bouvardin in field of health care products another object of the present invention is to provide preparation.
Application of the natural source bouvardin in cosmetic field another object of the present invention is to provide preparation.
Natural source bouvardin of the invention be from the secondary metabolite of plant extract be in plant synthesize tool
There are antipathogen or other activity, the class material with various structures type.Determined through Instrumental Analysis, its main component is included
Following species composition：Organic acid, terpenes, alkaloids, flavonoids, Antitoxins, steroidal, aldehydes, ketone, alcohols, phenol
Class, lignin, saponin, special acid, polypeptide, polysaccharide, Polyphenols, glycoprotein, ribosome inactivating protein class, furans and pyrrole
Mutter the active substance of plant such as derivative.Material is wherein had to be respectively：Acetic acid, propionic acid, 2- methoxy-phenols, 2- methoxyl groups -4-
Methyl-phenol, phenol, guaiacol, 4- methyl-phenols, 2,6- dimethoxy-phenic acids, Isosorbide-5-Nitrae：3,6- double dehydration-α-D- pyrans
Glucose, 2- methyl -2- cyclopentenones, 3- methyl -2- cyclopentenones and 4- hydroxy 3-methoxybenzene acetone.
Modern scientific research has confirmed：The compositions such as Polyphenols, flavonoids, acetic acid and phenols in mentioned kind composition
It is that there is very strong antifungal, bactericidal and antiviral active substance；For avian influenza virus, first, influenza B virus, B-mode
Hepatitis viruse (HBV), AIDS virus (HIV 1), SARS virus, human respiratory close spore precursor virus (RSV), gastroenteritis disease
Poison, hepatitis viruse, to bacillus, hay bacillus, Escherichia coli, pseudomonad, Gram-negative bacteria, gram-positive bacteria
Deng there is very strong inhibitory action；There is the effect such as anti-infective, antiallergy, anti-oxidant, antihaemorrhagics and blood vessel simultaneously；To blood fat
Protective effect (including their oxidisability)；The antioxidant action that polyphenols has shows as, and they can pass through
The lipid peroxidation reaction of free yl induction is removed to protect blood fat not hindered by oxidation.Evidence suggests polyphenol compound tool
Have it is anti-oxidant, reinforcing vascular wall, promote gastrointestinal disturbances, reduction blood fat fat and increase passive protective physical fitness, and with softening blood vessel,
Prevent artery sclerosis, thrombosis, reduce the effect such as cholesterol and triglyceride；Can also diuresis, hypotensive, suppression bacterium and cancer
Cell growth, and help digest.
Natural source bouvardin of the present invention is as follows as the application of fungicidal agents：
1) with deionized water dilute 1-5 times, pH value be 3.5~5, for various fungies, bacterial tinea pedis, the ringworm of the foot,
The dermatophytosis such as the tinea manuum, jock itch, ringworm of the body and women's colpomycosis；Infant and pregnant and lying-in women's swelling of buttock, dermatitis, skin
Tinea, itch, prickly heat, bite by mosquitos, vulva infection；And infant because excrement, urine, acid, alkali or growth of microorganism cause it is red
It is swollen to stimulate, diaper rash, with inhibiting bacteria and diminishing inflammation, sapor-removing efficacy.
2) with deionized water dilute 5-10 times, pH value be 4~6, for long-term bed patient due to stress point concentrate, grow
The stasis and immunity degradation of phase and the virus and bacterium infection that cause, and for bedsore and hemorrhoid, anal inflammation, anus
Door abscess, archorrhagia, can effective relief of symptoms.
3) 1-5 times is diluted with deionized water, pH value is 3.5~5, the sterilization, medical treatment for skin and mucosa are sterilized, family
In the room of front yard, office space sterilization, public health sterilization preventing and treating, refuse depot sterilization taste removal, animal particularly poultry disease it is pre-
The field such as anti-, food antiseptic and seasoning, fruit freshness preserving has application value, promotes and exploitation prospect is wide.
4) 2-6 times is diluted with deionized water, pH value is 3.5~5.5, can be used for the bacterium raising autoimmunity that prevents virus diseases
Oral health-care product, with significantly improving autoimmunity effect.
Natural source bouvardin scope of restraining fungi of the present invention is wide, to clinical common pathogenic bacteria such as Staphylococcus aureus
Bacterium, shigella dysenteriae, typhoid bacillus, proteus, aerobacteria, gonococcus, Klebsiella pneumoniae, pseudomonas aeruginosa and Escherichia coli
It is stronger etc. inhibitory action.Can also substantially suppress and kill Trichophyton rubrum, trichophyton gypseum, disconnected trichophyta, Epidermophvton
Tinea bacterium, Microsporum gypseum, microsporum lanosum；Also have to Candida albicans, sporotrichum schenckii and Fonsecaea pedrosor
There is obvious inhibiting effect, and effect compares without significant difference with chemobiotic.
The present invention using multistage UF membrane low temperature concentration extraction natural source bouvardin, it is to avoid high temperature is extracted to planting
The destruction of effective ingredient in thing, contained weak acid component, the inactivating efficacy not only for virus, fungi, bacterium is notable, weak acid component
Can easily be decomposed by human body, it is safe and harmless, and body immunity can be effectively improved, and also composition is clearly, the Genes For Plant Tolerance of preparation
The stable performance of raw element.
The present invention can rapidly be killed and be caused mould using the natural source bouvardin of multistage UF membrane low temperature concentration extraction
The staphylococcus aureus of various diseases such as bacterium property vaginitis, tinea pedis, infant thrush and Candida albicans.To various vaginas
Inflammation, skin disease, eczema, neurodermatitis, the tinea manuum, onychomycosis, curing psoriasis curative effect are obvious.
The present invention has reinforcing using the beverage that the natural source bouvardin of multistage UF membrane low temperature concentration extraction is made
Stomach, activating cell function, removal body fat and pre- anti-aging, hepatopathy, disease of skin, diabetes etc. have good result；Add
It is added in food and drink, with very strong scavenging activity, its SOD activity value is very high, internal active acid element is can remove, to what
The active acid element of the generations such as fatigue, smoking, satiety stress reaction, has good scavenging action, and plant addition natural source
The food of thing antibiotic obtains health care and prophylactic effect；Making an addition to has softening skin surface horny in cosmetics, kills
The good effects such as bacterium sterilization, skin care；Food antiseptic, fresh-keeping, storage etc. are played for food preservative and food additive agent
Aspect also has a wide range of applications；For wound mouthful, with healing of wound, anti-inflammatory, analgesic, antipruritic and detumescence effect.Additionally, day
Right source bouvardin has adjustment effect to plant growth, can suppress to hinder plant using natural source bouvardin in soil
The breeding of the microbiology class of growth, and the insects such as knot nematodes can be killed；Fertilizer, management of weeds can also be manufactured, promote plant life
Additive of the long, repellent as insect pest and feed stripped etc..
The invention has the advantages that：Natural source bouvardin has a broad antifungal spectrum of the present invention, to virus, very
Bacterium, bacterium inactivating efficacy significantly, safety and stability integrates treatment, prevention, health care, not only protects in bactericidal antiphlogistic simultaneously
Skin acid environment is not damaged, and can strengthen self purification, with mucous membrane wound healing is promoted, repairs pathological tissues enhancing
Natural defense function, can effectively prevent and treat infringement of the pathogenic microorganism to human body.Therefore, the present invention can be widely applied to
Prevention from suffering from the diseases, anti-ageing health care, skin nursing, amenities, cosmetics, daily chemical product, air sterillization, food, medicine, feed add
Plus agent, food additives, deodorant, soil conditioner, the prevention and control of plant diseases, pest control, raising vegetables and crop yield etc. and human lives
The closely related multiple fields of environment, with extensive value and DEVELOPMENT PROSPECT.
Brief description of the drawings
Fig. 1 is the process chart that the present invention prepares natural source bouvardin.
Fig. 2 is the GC-MS total ion current figures of the nanofiltration device initial outlet concentrated water of sample 1 in embodiment 1.
Fig. 3 is the GC-MS total ion current figures of the low pressure reverse osmosis device of sample 2 outlet fresh water in embodiment 1.
Specific embodiment
The following is specific embodiment of the invention, technical scheme is described further, but it is of the invention
Protection domain is not limited to these embodiments.It is every to be included in the present invention without departing substantially from the change of present inventive concept or equivalent substitute
Protection domain within.
Example 1
By Chinese hawthorn seed, red bayberry nucleocapsid, spina date seed shell, orange core, peach-pit shell, loquat seed, date nucleocapsid, almond shell, peach-pit
Shell, plum nucleocapsid, shaddock core, pine nut shell, the fruit stone of hazelnut shell, shell are added separately to retort for raw material, using hot tearing
Solution technology, initial stoste is collected according to the difference of above-mentioned raw material thermal cracking, and being divided into three phases is carried out, and is respectively 80~180
DEG C (RF1), 181~380 DEG C (RF2), 381~550 DEG C (RF3), the initial stoste that these three stages are collected into merges.
The initial stoste of merging that will be obtained according to above raw material again is in proportion：Chinese hawthorn seed 6.5, red bayberry nucleocapsid 6, spina date seed
Shell 5, orange core 4, peach-pit shell 7.5, loquat seed 7, date nucleocapsid 5.5, almond shell 6, peach-pit shell 6, plum nucleocapsid 4, shaddock core 5,
Pine nut shell 4, hazelnut shell 3.5 are mixed to get compound initial stoste；And compound initial stoste is stored in storagetank, 1-3 month is stood,
The effect such as allow labile element to aoxidize completely, be polymerized and precipitate, remove suspended material and other solid particles, taken with pumping
Middle level supernatant liquid, adds stoste pond, enters fine filtering device through raw water pump again after filtering, then successively through ultrafiltration apparatus, nanofiltration
Device, high pressure reverse osmosis membrane apparatus and low pressure reverse osmosis film device, control the corresponding concentrated water partial reflux of each device, adjust fluid
Flow velocity and operating pressure, adjusting process parameter, the stoste treating capacity for determining equipment stable operation is 300L/h.Membrane separation process is used
Low temperature is concentrated, and can to greatest extent retain the integrality of various biomass bodies.The present invention is using multistage film separation system to compound
Initial stoste carries out classification treatment, final to realize that the transparency liquid stablized is i.e. to compound initial stoste low temperature concentration point water
It is natural source bouvardin of the invention.Process chart is as shown in Figure 1.
Ultrafiltration apparatus, nanofiltration device, high pressure reverse osmosis unit and low pressure reverse osmosis device, adjusting process ginseng are opened successively
Number, when the stoste treating capacity for determining equipment stable operation is 300L/h, the process data of correspondence each several part is shown in Table 1.
The technological parameter of each device of correspondence when the stoste treating capacity of table 1 is 300L/h
Separating effect to films at different levels is investigated, using hierarchical pattern, respectively to nanofiltration device and reverse osmosis membrane apparatus
Operation of driving is carried out, nanofiltration device dope rate of discharge has been adjusted to without reflux state, respectively to nanofiltration device without circulation
Initial outlet concentrated water (sample 1) and low pressure reverse osmosis device export fresh water (sample 2) sampling and carry out GC-MS analyses, verify nanofiltration
The validity of film and reverse osmosis membrane.
Result and discussion：
GC-MS analyzes measurement result
Sample 1 and sample 2 is separately sampled after pretreatment measures, determines according to picture library retrieval and manual analysis spectrogram
Main component in sample, and percentage composition is determined according to area normalization method, the total ion current figure of sample is shown in Fig. 2 and Fig. 3, correspondence master
Composition percentage composition is wanted to be shown in Table 2 and table 3.
The main component of 2 sample of table 1 and content
The main component of 3 sample of table 2 and content
As can be known from Table 2, larger molecular organicses and small organic molecule are contained simultaneously in nanofiltration device outlet concentrated water, it is and first
Beginning stoste analysis result is consistent, illustrates do not have significant change through the liquid component after ultra-filtration and separation.Table 2 compares with table 3,
Result shows that be free of macromolecular compound after being separated through low pressure reverse osmosis device in fresh water, the content of micromolecular compound is also big
It is big to reduce, illustrate that the mixed liquor that the covering device can be effectively to initial stoste carries out concentration and a kind of isolated day of the invention
Right source bouvardin.
Example 2
By Chinese hawthorn seed, red bayberry nucleocapsid, spina date seed shell, orange core, peach-pit shell, loquat seed, date nucleocapsid, almond shell, peach-pit
Shell, plum nucleocapsid, shaddock core, pine nut shell, the fruit stone of hazelnut shell, shell are added separately to retort for raw material, using hot tearing
Solution technology, initial stoste is collected according to the difference of above-mentioned raw material thermal cracking, and being divided into three phases is carried out, and is respectively 80~180
DEG C (RF1), 181~380 DEG C (RF2), 381~550 DEG C (RF3), the initial stoste that these three stages are collected into merges.
The initial stoste of merging that will be obtained according to above raw material again is in proportion：Chinese hawthorn seed 4, red bayberry nucleocapsid 4.5, spina date seed
Shell 7.5, orange core 5, peach-pit shell 9.5, loquat seed 8, date nucleocapsid 5.3, almond shell 9.5, peach-pit shell 8, plum nucleocapsid 5, shaddock
Core 7, pine nut shell 6, hazelnut shell 6.5 are mixed to get compound initial stoste；And compound initial stoste is stored in storagetank, stand 1-3
Individual month, the effect such as allow labile element to aoxidize completely, be polymerized and precipitate, remove suspended material and other solid particles, use
Pumping takes middle level supernatant liquid, adds stoste pond, enters fine filtering device through raw water pump again after filtering, then successively through ultrafiltration dress
Put, nanofiltration device, high pressure reverse osmosis membrane apparatus and low pressure reverse osmosis film device, control the corresponding concentrated water partial reflux of each device,
Adjustment rate of flow of fluid and operating pressure, adjusting process parameter, the stoste treating capacity for determining equipment stable operation is 300L/h.Film point
Concentrated using low temperature from method, can to greatest extent retain the integrality of various biomass bodies.The present invention utilizes Multistage Membranes segregative line
System carries out classification treatment to being combined initial stoste, final to realize that is stablized is saturating to compound initial stoste low temperature concentration point water
Prescribed liquid is natural source bouvardin of the invention.
Embodiment 3
By Chinese hawthorn seed, red bayberry nucleocapsid, spina date seed shell, orange core, peach-pit shell, loquat seed, date nucleocapsid, almond shell, peach-pit
Shell, plum nucleocapsid, shaddock core, pine nut shell, the fruit stone of hazelnut shell, shell are added separately to retort for raw material, using hot tearing
Solution technology, initial stoste is collected according to the difference of above-mentioned raw material thermal cracking, and being divided into three phases is carried out, and is respectively 80~180
DEG C (RF1), 181~380 DEG C (RF2), 381~550 DEG C (RF3), the initial stoste that these three stages are collected into merges.
The initial stoste of merging that will be obtained according to above raw material again is in proportion：Chinese hawthorn seed 8.5, red bayberry nucleocapsid 7, spina date seed
Shell 4, orange core 7, peach-pit shell 5, loquat seed 5, date nucleocapsid 5, almond shell 5.5, peach-pit shell 9, plum nucleocapsid 6.8, shaddock core 4,
Pine nut shell 3.5, hazelnut shell 5 are mixed to get compound initial stoste；And compound initial stoste is stored in storagetank, 1-3 month is stood,
The effect such as allow labile element to aoxidize completely, be polymerized and precipitate, remove suspended material and other solid particles, taken with pumping
Middle level supernatant liquid, adds stoste pond, enters fine filtering device through raw water pump again after filtering, then successively through ultrafiltration apparatus, nanofiltration
Device, high pressure reverse osmosis membrane apparatus and low pressure reverse osmosis film device, control the corresponding concentrated water partial reflux of each device, adjust fluid
Flow velocity and operating pressure, adjusting process parameter, the stoste treating capacity for determining equipment stable operation is 300L/h.Membrane separation process is used
Low temperature is concentrated, and can to greatest extent retain the integrality of various biomass bodies.The present invention is using multistage film separation system to compound
Initial stoste carries out classification treatment, final to realize that the transparency liquid stablized is i.e. to compound initial stoste low temperature concentration point water
It is natural source bouvardin of the invention.
Embodiment 4
The measure of acetic acid contained by natural source bouvardin composition of the present invention：
1. instrument and reagent
Instrument：Shimadzu LG-1200 high performance liquid chromatographs, 975 type UV-detectors, C---R6A data processors.
Reagent：In addition to acetic acid is chromatographically pure, it is pure that other reagents are analysis；Silica GF254 is given birth to for Haiyang Chemical Plant, Qingdao
Produce.
2. chromatographic condition：
Analytical column：YMC---DDS---AQ (150mm × 6mm, 5u), 20ul proportional valves, Detection wavelength 225nm, mobile phase
0.2mol/L ammonium acetates --- methyl alcohol (85: 15), flow velocity 1.0ml/min, decay 6, chart speed cm/min, 0.02AuFs.
3. test and result：
It is accurate to weigh during 0.1 gram of acetic acid reference substance puts 100ml volumetric flasks, plus ethanol is diluted to scale, take respectively its 0.5,
1.0,1.5,2.0,2.5, in 3.0ml to 10ml volumetric flasks, plus ethanol is diluted to scale, is operated by above-mentioned chromatographic condition,
With peak area as ordinate, linear regression is carried out by abscissa of concentration, obtaining regression equation is：
Y=35.94x+0.393；R=0.9994
Result shows：Acetic acid concentration is in good linear relationship in the range of 0.05~0.30mg/ml
Sample treatment：Natural source bouvardin 5ml of the present invention is taken, in putting separatory funnel, 10,5,5ml is added diethyl ether successively
Extract, merge extract solution, be evaporated to dryness in water-bath, residue ethanol dissolves, and lysate is put in 100ml volumetric flasks, plus ethanol is extremely
Scale, it is standby.
The preparation of reference substance solution：It is accurate to weigh during acetic acid reference substance 0.1g puts 100ml volumetric flasks, plus ethanol is diluted to quarter
Degree, takes during this liquid 2.0ml puts 10ml volumetric flasks, plus ethanol is diluted to scale, standby.
3 batches, the sample of assay Example 1-3, is determined by above-mentioned chromatographic condition, calculates content, and phenol is with mg/ml
The percentage composition of meter is 18.61%, 18.83%, 18.85%.
4. recovery test：
The natural source bouvardin preparation 3.6g of known content is taken, accurately weighed, totally 6 parts, difference is accurate to add phenol
Contrast solution (7.0531mg/ml-1) 1ml, puts in 250ml round-bottomed flasks, and add water 150ml, volatile oil determination apparatus is connected, according to confession
Method treatment is determined under test product preparation, and average recovery rate is that 99.58%, RSD is 0.17%.
Embodiment 5
The measure of natural source bouvardin ingredient phenol of the present invention：
1st, instrument and material
GC-122 gas chromatographs, phenol, Tianjin light is produced into chemical reagent Co., Ltd, natural source bouvardin
The analysis of (self-control) naphthalene, ethyl acetate and anhydrous sodium sulfate is pure.
2nd, method and result
Chromatographic condition chromatographic column is polyethylene glycol (PEG) -20M, 120 DEG C of column temperature；Gasification room temperature is 240 DEG C；Detector；
Flame ionization ditector, temperature is 280 DEG C, flow velocity 1.5ml/min-1；H2 flow velocitys 30ml/min-1；Air velocity 350ml/
min-1。
Correction factor is determined and takes naphthalene 100mg, accurately weighed, plus ethyl acetate is made solution of every 1ml containing 100mg, as
Inner mark solution.It is another to take phenol reference substance 40mg, it is accurately weighed, plus ethyl acetate is made solution of every 1ml containing phenol 4g, as right
According to product solution, precision measures reference substance solution and each 1ml of inner mark solution, and in putting 10ml measuring bottles, plus ethyl acetate is to scale, shakes
1 μ l of even, smart absorption inject gas chromatograph, and continuous sample introduction 5 times is calculated compared with positive divisor by average peak area.
The preparation of need testing solution takes sample natural source bouvardin preparation, accurately weighed, takes about 8g, accurately weighed,
Put in 250ml round-bottomed flasks, add water 150ml, shakes up, connect volatile oil determination apparatus, being added water from analyzer upper end makes full of scale
Part, adds ethyl acetate 4ml connection reflux condensing tubes, is heated to reflux 4h, lets cool, and liquid in volatile oil determination apparatus is moved to
In separatory funnel, condenser pipe is washed with a small amount of ethyl acetate, be incorporated to separatory funnel, point take ethyl acetate layer, with being covered with anhydrous sulphur
The sintered glass funnel filtration of sour sodium 0.6g, filtrate is put in 10ml measuring bottles, and molten device is washed with a small amount of ethyl acetate, and washing lotion is incorporated to together
In one bottle, precision adds 1ml inner mark solutions, plus ethyl acetate is to scale, shakes up, obtain final product, and precision draws need testing solution 1ml,
Injection gas chromatograph, determines.
Linear relationship investigates precision and weighs phenol reference substance 220mg, and in putting 25ml measuring bottles, plus ethyl acetate dissolves and dilutes
To scale, then respectively precision pipette 1,3,5,7, during 9ml puts 100ml measuring bottles, plus ethyl acetate is diluted to scale, shakes up, as
Reference substance solution, draws above-mentioned reference substance solution 1ml respectively, injects gas chromatograph, determines peak area, and concentration is carried out with phenol
It is abscissa, peak area is ordinate, makes standard curve, obtains the side's of recurrence section：C=56.12x+0.435；R=0.9999, table
Bright phenol linear relationship in 5~50ug/ml concentration ranges is good.
Precision test draws the μ l of reference substance solution 1 and repeats sample introduction 5 times, determines peak area, RSD=0.2% (n=5).
Reappearance test is taken with batch 5 parts of sample, is prepared by under test sample preparation respectively, surveys phenol content, RSD=
0.3% (n=5).
Blank control test prepares empty preparation in prescription ratio and technique, and blank is obtained with test liquid preparation method
Solution, according to the lower method test of assay, takes reference substance, sample and each 1 μ l injections gas chromatograph of placebo solution, note
Chromatogram is recorded, as a result negative sample solution does not disturb the assay of phenol.
Stability test is taken with portion need testing solution, is determined peak area, and is calculated respectively at 0,2,4,6,8,12h sample introductions
RSD=0.9%, shows that sample liquid is good in 12h internal stabilities.
Recovery test takes the natural source bouvardin preparation 3.6g that second knows content, accurately weighed, totally 6 parts, difference essence
Close addition phenol contrast solution (7mg/ml-1) 1ml, puts in 250ml round-bottomed flasks, and add water 150ml, connects volatile oil determination apparatus,
Determined according to method treatment under test sample preparation, average recovery rate is that 99.76%, RSD is 0.27%.
3 batches, the sample of assay Example 1-3, is determined by above-mentioned chromatographic condition, calculates content, and phenol is with mg/ml
The percentage composition of meter is 7.04%, 7.15%, 7.18%.
Embodiment 6：Natural source bouvardin of the present invention as foliar fertilizer administration, the administration result table on strawberry
Bright, the natural source bouvardin knapsack sprayer after 200 times of dilution is sprayed in Strawberry Leaves, harvests total amount and number is equal
It is dramatically increased, pol is also significantly improved.
Embodiment 7：Natural source bouvardin of the present invention is used for the prevention and control of plant diseases, pest control, and natural source bouvardin is diluted into 50
Pest control is sprayed on crop after times, is soaked seed with this kind of dilution, irrigation before seeding is carried out with 500 times of dilutions can replace native bacterium
Disappear, the agricultural chemicals such as Bravo is implemented without pesticide control.
Embodiment 8：Natural source bouvardin of the present invention is used for cosmetics and dilutes 30 times afterwards with softening skin surface angle
The good effects such as matter, sterilizing, skin care；There is the functions such as cleaning skin and sterilization, sterilization, anti-inflammatory simultaneously.
Embodiment 9：Natural source bouvardin of the present invention dilutes 15 times and is used to festering afterwards skin anti-inflammation and sterilization and quickly to be repaiied
Fester again face, can rapidly play a part of anti-inflammation and sterilization, and the face of festering be able to will form a scab healing in 1~2 day.
Embodiment 10：Natural source bouvardin of the present invention dilutes 10 times has body odour position, two to three days for body afterwards
Interior, body odour is just disappeared.
BA
BA uses filter paper enzyme and solid dilution method.Using gentamicin as control, natural source Genes For Plant Tolerance is determined
Raw element is to Escherichia coli, proteus, bacillus megaterium, aerobacteria, staphylococcus aureus and Corynebacterium Pekinese
Bactericidal action；It is control with benzene azoles mould, determines natural source bouvardin to black-koji mould, Penicillium notatum, green trichoderma bacterium, Kang Shi
Trichoderma and the bactericidal action of Candida albicans.Bactericidal effect to bacterium is as shown in table 4.To the bactericidal effect such as table 5 of mould
It is shown.
The natural source bouvardin of table 4 is compareed with gentamicin sterilization
Note：Data are sterilized loop diameter in table, and unit is mm, is 3 average values of parallel test；Level of signifiance P ＜
0.05。
The natural source bouvardin of table 5 is compareed with the sterilization of benzene azoles mould
Note：Data are sterilized loop diameter in table, and unit is mm, is 3 average values of parallel test；Level of signifiance P ＜
0.05。
Make sterilized control according to upper table 4 and gentamicin, table 5 and benzene azoles mould, natural source bouvardin to supplying
Control sample is significantly better than between trying bacterium, the killing effect of mould and compareing.Therefore, natural source plant antibiotic, with
The aspects such as medicine, food, amenities, cosmetics, cultivation, biological pesticide have the extensive value for developing.
Skin Irritation Test:
1. animal is tested：Healthy rabbits 6, each 3 of male and female, body weight 2.5kg, skin is intact.
2. test：The 24h before experiment, the hair of family's rabbit back backbone both sides is removed, unhairing scope 4cm × 4cm, and next day will
Natural source bouvardin of the present invention is directly dripped in area on the side unhairing intact skin of 3cm × 3cm；Opposite side is with originally
Water is compareed.Respectively at 1h, 24h and 48h observation local skin reaction after experiment.
3. experimental result：1 rabbit has slight erythema, and 5 rabbit are without oedema formation, nonirritant reaction, without skin
Skin allergic reaction.
Toxicity test：
The toxicity research that rat is injected has been carried out to the natural source bouvardin, it is as a result as follows：
Sample extraction and setting：Rat 60 is taken at random, the dosage of rat is calculated by kg body weight.
One group 30, rat is injected this product and is respectively equivalent to 50 times of clinical people's consumption (0.5ml), 25 times, 5 times, often
1 dosage is adjusted by body weight within 18 days, medicine is put daily 1 time；Medicine is put altogether 72 times (72 days).
Two groups 30,20 times that this product is respectively equivalent to clinical human's consumption, 10 times, 3 times, every 18 days are injected to rat
1 dosage is adjusted by body weight, daily injection 1 time；Co-injection 72 times (72 days).
Observe the general status of rat：To each group rat before with injection, in and stop injection after 24 hours, 20 days
Survey body weight, routine of having a blood test, ALT (GPT), BUN and stool and urine routine；Stop injection 24 hours put to death 50% animal, core, lung,
Liver, spleen, stomach, intestine and small intestine, brain, lymphonodi mesenterici；Remaining animal stops injection and all puts to death for 20 days, as above does pathologic finding.
Obtain a result：Each group rat general status and outward appearance are normal during usually situation and body weight are tested, before and after injection
Its activity, feed, hair color, body weight, the equal no significant difference of stool and urine, body weight are significantly increased, and also indifference is compared with control group
It is different.Illustrate the product on the general status and body weight of animal without influence.
Blood routine examination：Injection 36 days, 72 days, stop injection 24 hours and stop injection 20 days after each dosage group rat it is red
Cell, white blood cell count(WBC) and classification, hemoglobin, reticulocyte count are compared with control group, and there are no significant for difference.
ALT and BUN is checked：Each detection time point, each dosage group ALT, BUN compare with control group, and there are no significant for difference.
Pathologic finding：The rat that injection 72 days and stopping injection being put to death for 20 days, dissection is visually observed and is showed no exception.It is all
The organ specimens sample of censorship, compares through pathologic finding with control group, and there are no significant for difference.
According to the product toxicity in animals experimental data result, show natural source bouvardin be it is a kind of it is nontoxic, without pair
Effect and the invention of safety.
Killing effect of the natural source bouvardin of the present invention to hepatitis B surface antigen (HBsAg) is determined using SPRIA methods：
Take 0.1ml hepatitis B surface antigen (HBsAg) positive serum (RPHA methods 1：2048) it is added on 0.4ml various concentrations this hairs
Bright bouvardin, effect 2 points, 5 points, after 10 minutes immediately plus nertralizer act on 30 minutes terminate experiment.The positive is set simultaneously (no
Plus bouvardin and add physiological saline) it is and negative (be not added with hepatitis B surface antigen (HBsAg) positive serum and plus physiological saline).
Be judged to effectively with P/N value≤2.1 feminine gender, >=2.1 for the positive be judged to it is invalid.
Result of the test shows that 50~100% concentration, effect has sterilization to imitate for 5~10 minutes to hepatitis B surface antigen (HBsAg)
Really.
Stability test shows：It is right after natural source bouvardin preparation stoste of the present invention is deposited 90 days through 37 DEG C of constant temperature
The killing action of Candida albicans is without influence.
Claims (9)
1. a kind of preparation method of natural source bouvardin, it is characterised in that comprise the following steps：
1) by Chinese hawthorn seed, red bayberry nucleocapsid, spina date seed shell, orange core, peach-pit shell, loquat seed, date nucleocapsid, almond shell, peach-pit shell,
Plum nucleocapsid, shaddock core, pine nut shell, hazelnut shell are added separately to retort for raw material, using pyrolysis technology, according to above-mentioned
The difference of raw material thermal cracking collects initial stoste, and being divided into three phases is carried out, be respectively 80~180 DEG C, 181~380 DEG C,
381~550 DEG C, by the initial stoste that every kind of raw material three phases are collected into merge respectively every kind of raw material merging it is initial
Stoste；
2) by step 1) the initial stoste of merging of every kind of raw material that obtains proportionally mixes, obtains being combined initial stoste；
3) by step 2) the compound initial stoste that obtains is stored in storagetank and stands 1-3 month, allows labile element that oxygen occurs completely
Change, polymerization and precipitate, remove suspended material and other solid particles, take middle level supernatant liquid with pumping, add stoste pond,
Enter fine filtering device through raw water pump again after filtering, then successively through ultrafiltration apparatus, nanofiltration device, high pressure reverse osmosis membrane apparatus and low
Pressure reverse osmosis membrane apparatus, control the corresponding concentrated water partial reflux of each device, adjust rate of flow of fluid and operating pressure, adjusting process ginseng
Number, determines the stoste treating capacity of equipment stable operation for 300L/h, to compound initial stoste low temperature concentration point water, is stablized
Transparency liquid is natural source bouvardin.
2. the preparation method of natural source bouvardin as claimed in claim 1, it is characterised in that the step 2) in it is every kind of
The ratio of the initial stoste of merging of raw material is in parts by weight, specific as follows：Chinese hawthorn seed 4-8.5 parts, 4.5-7 parts of red bayberry nucleocapsid,
4-7.5 parts of spina date seed shell, 4-7 parts of orange core, 5-9.5 parts of peach-pit shell, loquat seed 5-8 parts, 5-5.5 parts of date nucleocapsid, almond shell
5.5-9.5 parts, 6-9 parts of peach-pit shell, 4-6.8 parts of plum nucleocapsid, 4-7 parts of shaddock core, 3.5-6 parts of pine nut shell, hazelnut shell 3.5-6.5
Part.
3. the preparation method of natural source bouvardin as claimed in claim 1, it is characterised in that the step 3) in be obtained
Natural source bouvardin with phenol and organic acid content meter, wherein phenols is not less than 7.04% in terms of phenol content；It is organic
Acids is not less than 18.61% in terms of acetic acid content, and density is 1.0033；PH value is 1.5~3.0.
4. the natural source bouvardin for being prepared by the use of any described preparation methods of claim 1-3 is used as antifungal, bacterium
The application of medicine.
5. natural source bouvardin the answering in anti-virus aspect for being prepared using any described preparation methods of claim 1-3
With.
6. using the natural source bouvardin of any described preparation method preparations of claim 1-3 in terms of plant growth
Using.
7. the natural source bouvardin for being prepared by the use of any described preparation methods of claim 1-3 is used as feed addictive
Using.
8. natural source bouvardin the answering in field of health care products for being prepared using any described preparation methods of claim 1-3
With.
9. natural source bouvardin the answering in cosmetic field for being prepared using any described preparation methods of claim 1-3
With.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108617709A (en) * | 2018-05-25 | 2018-10-09 | 东营润益生物科技有限公司 | A kind of preparation of novel pure plant based bacteriostat and application process |
| CN108926612A (en) * | 2018-06-05 | 2018-12-04 | 天津果核生物科技有限公司 | A kind of preparation method of bouvardin |
| CN114680141A (en) * | 2020-12-30 | 2022-07-01 | 山东步长神州制药有限公司 | Use of a natural composition as a disinfectant in blocking the transmission of viruses through packaging surfaces |
| CN115105551A (en) * | 2022-06-30 | 2022-09-27 | 任永飞 | Plant type medicine for inhibiting novel coronavirus and preparation method thereof |
| CN115252693A (en) * | 2022-06-06 | 2022-11-01 | 任永飞 | Medicine of plant type inhibitory transmission virus HIV-1 and HSV-2 and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1883275A (en) * | 2006-06-02 | 2006-12-27 | 杨先锋 | A botanical antibiotic and application thereof |
-
2017
- 2017-04-18 CN CN201710250940.XA patent/CN106860647A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1883275A (en) * | 2006-06-02 | 2006-12-27 | 杨先锋 | A botanical antibiotic and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 乔玉彦等: "膜浓缩木醋液的工艺研究", 《河南化工》 * |
| 尉芹等: "核桃壳木醋液的制取、成分分析及抑菌试验", 《农业工程学报》 * |
| 蔡玉英等: "瓦松栓对大鼠的阴栓试验", 《卫生毒理学杂志》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108617709A (en) * | 2018-05-25 | 2018-10-09 | 东营润益生物科技有限公司 | A kind of preparation of novel pure plant based bacteriostat and application process |
| CN108926612A (en) * | 2018-06-05 | 2018-12-04 | 天津果核生物科技有限公司 | A kind of preparation method of bouvardin |
| CN108926612B (en) * | 2018-06-05 | 2021-02-26 | 果核(天津)生物医药科技有限公司 | Preparation method of plant antibiotic |
| CN114680141A (en) * | 2020-12-30 | 2022-07-01 | 山东步长神州制药有限公司 | Use of a natural composition as a disinfectant in blocking the transmission of viruses through packaging surfaces |
| CN115252693A (en) * | 2022-06-06 | 2022-11-01 | 任永飞 | Medicine of plant type inhibitory transmission virus HIV-1 and HSV-2 and preparation method thereof |
| CN115105551A (en) * | 2022-06-30 | 2022-09-27 | 任永飞 | Plant type medicine for inhibiting novel coronavirus and preparation method thereof |
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