CN106135298A - Radix Carpesii extract and its preparation method and application - Google Patents

Abstract

The invention discloses Tianmingjing extract, its preparation method and application. The Tianmingjing fungicide of the present invention is safe to the environment, and the active compound in the Tianmingjing fungicide is a naturally occurring substance, derived from nature, and has formed its inherent pathway of participating in energy and material cycle metabolism during the long-term evolution process , so there is no residue in the environment or on plants, and it will not cause bioaccumulation; it is relatively safe for non-target organisms; the mechanism of action on plant pathogens is different from conventional pesticides, and pathogens are not easy to develop resistance to them after use. Resistance to conventional fungicides also has a delaying effect.

Description

Tianmingjing extract and its preparation method and application

technical field

The invention relates to plant extracts and applications thereof, in particular to Tianmingjing extracts, preparation methods and applications thereof.

Background technique

Since the 1940s, chemical pesticides have dominated the pesticide market due to their variety, quick effect, convenient use, and low price. However, due to people's irrational use of chemical pesticides and the inherent shortcomings of pesticides. As a result, problems such as environmental pollution, human and animal poisoning, and pesticide residues have occurred in the use of chemical pesticides. With the improvement of people's quality of life and the enhancement of environmental awareness, the development of bio-pesticides that are safe for humans and animals, non-polluting, low-toxic, and low-residue has become a new hot spot in the field of pesticide research. As a part of biologically rational pesticides, the development and research of botanical pesticides has always been one of the hot topics in the research of new pesticides. Botanical pesticides are derived from nature and can be degraded in nature. Generally, they do not pollute the environment and agricultural products, and are less likely to accumulate toxicity in the environment and the human body. They are relatively safe for humans and livestock, have little damage to natural enemies of pests, and have low toxicity, The characteristics of low residue can maintain the high quality of agricultural products, so botanical pesticides have a broad market.

In recent years, many scholars at home and abroad have investigated and studied the antibacterial and bactericidal activities of some plant extracts. Some studies have shown that plant-derived antibacterial (killing) substances can act on multiple structures of bacterial cells, and the risk of microbial resistance is small.

Botanical fungicides have the advantages of broad spectrum of action, no obvious vertebrate toxicity or phytotoxicity, rapid environmental degradation, abundant resources, and renewable, so the development and application of botanical fungicides have very broad prospects.

Contents of the invention

In order to solve the deficiencies of the prior art, the present invention provides Tianmingjing extract and its preparation method and application.

The technical solution of the present invention is: Tianmingjing extract, which is obtained after extracting the roots, stems, leaves and/or seeds of Tianmingjing.

The preparation method of Tianmingjing extract comprises the following steps: step 1, crushing dried Tianmingjing roots, stems, leaves and/or seeds, adding 80% to 95% ethanol for extraction, collecting the filtrate to obtain Tianmingjing The crude extract; step 2, the crude extract of Tianmingjing is extracted with ethyl acetate to obtain the antibacterial extract of Tianmingjing.

Further improvements of the present invention include:

The step 1 is repeated for several times until the color of the extract does not change, the filtrates are combined to obtain an extract, and the extract is concentrated into a dilute extract to obtain a crude extract.

In the step 2, the crude extract of Tianmingjing is extracted with ethyl acetate, and the ester extract is depigmented with a macroporous adsorption resin, and then purified with ethyl acetate.

The present invention further provides the application of Tianmingjing extract in the preparation of medicines for preventing and treating Botrytis cinerea, tomato leaf mold, tomato early blight, cucumber wilt, potato early blight and/or capsicum phytophthora.

The present invention also provides a preparation method of a bactericidal microemulsion containing Tianmingjing extract. In a reaction kettle, first dissolve Tianmingjing extract and pesticide adjuvant in ethyl acetate solvent, and heat Under the condition of high-speed stirring, add the obtained solvent into distilled water, and keep stirring for 1 hour to obtain Tianmingjing bactericidal microemulsion.

The bactericidal microemulsion containing Tianmingjing extract prepared according to the above method.

The bactericidal microemulsion containing Tianmingjing extract comprises: 1-3 parts of Tianmingjing extract, 1-2 parts of solvent, 3-5 parts of water, and 1-2 parts of pesticide adjuvant according to weight percentage.

Compared with the fungicides commonly used in the prior art, the present invention has the following characteristics:

1. Environmental safety The active compounds in Tianmingjing fungicides are naturally occurring substances, which are derived from nature. During the long-term evolution process, they have formed their inherent metabolic pathways to participate in energy and material circulation, so the application is compatible with the environment or On plants, no residues, will not cause bioconcentration:

2. Relatively safe to non-target organisms;

3. The mechanism of action on plant pathogenic bacteria is different from that of conventional pesticides. After use, pathogenic bacteria are not easy to develop resistance to it, and it can also delay the resistance of pathogenic bacteria to conventional fungicides.

The Tianmingjing fungicide of the present invention is safe to the environment, and the active compound in the Tianmingjing fungicide is a naturally occurring substance, derived from nature, and has formed its inherent pathway of participating in energy and material cycle metabolism during the long-term evolution process , so when applied in the environment or on plants, there is no residue and will not cause bioaccumulation; it is relatively safe to non-target organisms; the mechanism of action on plant pathogenic bacteria is different from conventional pesticides, and pathogenic bacteria are not easy to develop resistance to it after use. Resistance to conventional fungicides also has a delaying effect.

detailed description

The present invention will be described in detail below in conjunction with the embodiments.

Example 1: Preparation of Tianmingjing Antibacterial Active Extract:

Take 100kg of Tianming refined dry sample, add 70% ethanol for reflux extraction twice, add 800kg 70% ethanol for the first time, add 600kg 70% ethanol for the second time, filter, combine the extracts, reclaim the ethanol from the extracts and concentrate to Dilute extract with a relative density of 1.05, extract the thin extract with ethyl acetate to obtain an ethyl acetate phase, pass the ethyl acetate phase through D101 macroporous adsorption resin, and first elute with 30% ethanol until it is colorless. The % ethanol eluate was discarded, and then eluted with 85% ethanol until it was colorless, the 85% ethanol eluate was collected, the ethanol was recovered and concentrated into a thick paste with a relative density of 1.25, and dried in vacuum to obtain Tianmingjing Antibacterial extract (according to lactone, the mass fraction is not less than 60%).

Embodiment 2: the preparation of Tianmingjing bactericidal microemulsion:

Add 2 parts of Tianmingjing’s antibacterial and refined extract to the adjusted stirring reaction kettle, dissolve 1 part of the pesticide adjuvant Termul 5030 with 2 parts of ethyl acetate, stir at a high speed at a temperature of 40°C to 50°C, and add deionized 3 parts of water, stirred at constant temperature for 1 hour, and 40% Tianmingjing bactericidal microemulsion was obtained.

Embodiment 3: Determination of Antibacterial Activity of Tianming Fine Microemulsion

Dilute 0.02 mL of Tianming fine microemulsion prepared in Example 2 to 10 mL with melted PDA medium, mix well and pour it into a petri dish to prepare a plate with bacteria. Test the pathogenic bacteria with plate culture in advance for 5-7 days, use a sterilized hole puncher to make a bacterium cake (diameter is 4mm) on the bacterium colony that has been cultivated, then buckle the bacterium cake upside down in the center of the mixed medicine flat plate, and repeat each treatment 3 times. Set an equal amount of sterilized water as a control. The inoculated strain plate was placed in a constant temperature incubator (25±1°C) for cultivation, and the colony diameter was measured for 72 hours by the cross method, and the inhibition rate was calculated according to the following formula. The results are shown in Table 1.

Colony growth diameter = colony diameter -4

Table 1 Antibacterial activity of Tianming microemulsion

Note: the data in the table is the average value of three repetitions, and different letters after the data in the same column indicate significant differences between the data (P<0.05, Duncan's new multiple range method); "-" indicates that no antibacterial activity was detected.

The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments, and what described in the above-mentioned embodiments and the description only illustrates the principles of the present invention, and the present invention will also have other functions without departing from the spirit and scope of the present invention. Variations and improvements are possible, which fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.

Claims (8)

1. Tianmingjing extract, which is characterized in that it is prepared after extracting the roots, stems, leaves and/or seeds of Tianmingjing. 2. The preparation method of Tianmingjing extract is characterized in that it comprises the following steps: Step 1, the dried Tianmingjing roots, stems, leaves and/or seeds are pulverized, and 80% to 95% of ethanol is added to extract, The filtrate is collected to obtain the crude extract of Tianmingjing; step 2, the crude extract of Tianmingjing is extracted with ethyl acetate, and the antibacterial extract of Tianmingjing is obtained. 3. the preparation method of Tianmingjing extract according to claim 2, is characterized in that, described step 1 repeatedly extracts repeatedly to the extract solution color and no longer changes, merges filtrate to obtain extract, and this extract Concentrated into dilute extract, get the name of fine crude extract. 4. the preparation method of Tianmingjing extract according to claim 2, is characterized in that, in described step 2, Tianmingjing crude extract extracts ester extract through macroporous adsorption resin to remove pigment after ethyl acetate extraction , and then purified by ethyl acetate. 5. Application of Tianmingjing extract in the preparation of medicines for preventing and treating Botrytis cinerea, tomato leaf mold, tomato early blight, cucumber wilt, potato early blight and/or capsicum phytophthora. 6. The preparation method of the bactericidal microemulsion containing the Tianmingjing extract is characterized in that, in the reaction kettle, the Tianmingjing extract and the pesticide adjuvant are dissolved in ethyl acetate solvent and heated at 40°C to 50°C Under the condition of high-speed stirring, add the obtained solvent into distilled water, and keep stirring for 1 hour to obtain Tianmingjing bactericidal microemulsion. 7. The bactericidal microemulsion containing Tianmingjing extract is characterized in that it is prepared according to the method of claim 6. 8. The bactericidal microemulsion containing Tianmingjing extract is characterized in that, according to weight percentage, it includes: 1-3 parts of Tianmingjing extract, 1-2 parts of solvent, 3-5 parts of water, and 1-3 parts of pesticide adjuvant 2 servings.