CN100381058C - A seed treatment agent for promoting seed germination - Google Patents

Abstract

The invention discloses a seed treatment agent for promoting seed germination. The present invention mainly contains gibberellin, naphthalene acetic acid, zeatin and carbendazim, and the rest is water; in addition, the present invention can also contain three kinds of water extracts of tamarisk root or water extract of Haloxylon root or water extract of Nitraria root One of the plant root extracts. The present invention overcomes the problems of difficult germination and large dosage of Cistanche plant seeds, uses the seed treatment agent of the present invention to significantly promote the germination of Cistanche plant seeds, and can increase its germination rate to more than 75%. , Haloxylon root water extract and Nitraria root water extract are all natural products extracted from plants, which are safe to use and environmentally friendly. The preparation method of the seed treatment agent of the invention is simple and widely used.

Description

A seed treatment agent for promoting seed germination

technical field

The invention relates to a seed treatment agent, in particular to a seed treatment agent for promoting seed germination.

Background technique

Cistanche deserticola, commonly known as Dayun, is a genus of Liedangaceae. Cistanche deserticola is a perennial high-level parasitic herb that cannot perform photosynthesis to produce organic matter. It mainly parasitizes on the roots of Haloxylon, Tamarix and other plants to absorb water and nutrients from the host. . In my country, Cistanche is mainly distributed in saline-alkali land, dry river ditches or Gobi Desert in Inner Mongolia, Shaanxi, Gansu, Ningxia and Xinjiang. In my country, there are mainly 6 species of Cistanche saline, Cistanche, Cistanche tuberosa, Lanzhou Cistanche, Cistanche cistanche, and Cistanche minicola, and one variety of Cistanche alba. Among them, Cistanche tuberosa is the main source plant of the domestic traditional Chinese medicine Cistanche.

Cistanche is a traditional Chinese herbal medicine. It has the functions of tonifying the kidney, nourishing the essence, strengthening the tendons and strengthening the marrow. It is mainly used to treat the weakness of the lower part and dry blood in old age. Taking it for a long time can strengthen the body. In recent years, it is often used as an anti-aging and anti-cancer drug. With the continuous improvement of living standards, people's self-care awareness is enhanced, and the health care products made of Chinese herbal medicine Cistanche are favored by people. There is more and more demand for Cistanche, which has caused the extinction of wild Cistanche resources Non-renewable mining has destroyed non-renewable resources and the ecological environment, but it still cannot meet people's needs. Artificial cultivation of Cistanche is the main way to solve this problem, and the patent application "a method for inoculating the stem of Tamarix cistanche" (patent application number is: 200610087305.6) just provides a kind of method of artificial cultivation of Cistanche tuberosa. But to cultivate Cistanche deserticola at first needs high-quality Cistanche deserticola seeds as propagation material, due to the parasitic characteristics of Cistanche deserticola, its seeds are difficult to germinate under the condition of no host root (Li Tianran, 1996; Guo Yuhai et al., 2005; Niu Dongling et al., 2006), this It is very difficult to determine the seeding amount before sowing, which has led to a particularly large seeding amount of Cistanche deserticola at present, sometimes as many as 180 seeds are placed in one hole. The price, taking Cistanche tuberosum as an example, is now as high as 5,000 yuan per kilogram of Cistanche tuberosum seeds. To solve these problems, there is an urgent need for a method that can effectively promote the germination of Cistanche plant seeds. It is found through retrieval that the patent application "Cotton Rooting and Seedling Strengthening Agent" (application number: 03152893.7) discloses a combination of naphthaleneacetic acid and indolebutyric acid , carbendazim, ammonium hydroxide, sodium hydroxide and water are used to promote cotton rooting; It is a seed treatment agent for promoting seed germination, which is composed of amycin, indole acetic acid, naphthyloxyacetic acid, paclobutrazol, etc. But so far, there is no discovery about the seed treatment agent that can promote the seeds of Cistanche genus plants composed of gibberellin, naphthaleneacetic acid, zeatin and carbendazim.

Contents of the invention

The object of the present invention is to provide a seed treatment agent for seed germination which has easy-to-obtain raw materials, simple production process, low cost, high efficiency, low toxicity and environmental friendliness.

The object of the invention is achieved through the following technical solutions:

A seed treatment agent for promoting seed germination, containing gibberellin, naphthaleneacetic acid, zeatin and carbendazim.

The composition and weight percentage of above-mentioned seed treatment agent are:

Gibberellin 0.0001%～0.1%;

Naphthaleneacetic acid 0.0001%～0.1%;

Zeatin 0.0001%～0.1%;

Carbendazim 0.1% to 2%;

The rest is water.

The above-mentioned seed treatment agent may also contain the water extract of Tamarix root, or Haloxylon root water extract, or one of the three kinds of water extracts in the root water extract of Nitraria japonicus.

The above-mentioned seed treatment agent can be composed of gibberellin, naphthaleneacetic acid, zeatin, carbendazim and tamarisk root water extract, and the rest is water.

The composition of above-mentioned seed treatment agent and percentage by weight thereof can be:

Gibberellin 0.0001%～0.1%;

Naphthaleneacetic acid 0.0001%～0.1%;

Zeatin 0.0001%～0.1%;

Carbendazim 0.1% to 2%;

Tamarix root water extract 0.001% to 2%;

The rest is water.

The above-mentioned seed treatment agent can be composed of gibberellin, naphthaleneacetic acid, zeatin, carbendazim and Haloxylon root water extract, and the rest is water.

The composition of above-mentioned seed treatment agent and percentage by weight thereof can also be:

Gibberellin 0.0001%～0.1%;

Naphthaleneacetic acid 0.0001%～0.1%;

Zeatin 0.0001%～0.1%;

Carbendazim 0.1% to 2%;

Haloxylon root water extract 0.001% ~ 2%;

The rest is water.

The above-mentioned seed treatment agent can be composed of gibberellin, naphthaleneacetic acid, zeatin, carbendazim and the root water extract of Nitraria japonicus, and the rest is water.

The composition of above-mentioned seed treatment agent and percentage by weight thereof also can be:

Gibberellin 0.0001%～0.1%;

Naphthaleneacetic acid 0.0001%～0.1%;

Zeatin 0.0001%～0.1%;

Carbendazim 0.1% to 2%;

Water extract of the root of the white thorn 0.001% ~ 2%;

The rest is water.

The present invention also provides the application of the above-mentioned seed treatment agent in promoting the germination of the seeds of the succulent plants.

The raw materials used in the present invention are introduced as follows:

Gibberellin:

English name: gibberellin

Molecular formula: C ₁₉ H ₂₂ O ₆ ; molecular weight: 346.37

Its structural formula:

Gibberellin GA ₃ is the most widely used plant growth regulator in agriculture, forestry and horticulture in China. The physiological effects of gibberellin GA ₃ mainly include: changing the ratio of female and male flowers in some crops, inducing parthenocarpy, accelerating fruit growth, and promoting fruit setting; breaking seed dormancy, early seed germination, accelerating stem elongation and the growth of some crops Bolting; expand leaf area, accelerate young shoot growth, facilitate the accumulation of metabolites in the phloem, activate cambium; inhibit maturation and aging, control lateral bud dormancy and tuber formation.

Physical properties: Pure gibberellin is generally a colorless crystalline powder, easily soluble in ethanol and other alcohols, acetone, glacial acetic acid, and pH 6.2 phosphate buffer, but insoluble in water, benzene, and chloroform. Generally speaking, gibberellin is relatively stable at low temperature and moderately acidic conditions.

Naphthaleneacetic acid

English name: a-naphthylacetic acid; NAA; Celmone; Alphaspra; Rootone; Fruitofix; Parmone; Phyomone

Molecular formula: C ₁₂ H ₁₀ O ₂ ; molecular weight: 186.2;

Its structural formula:

It is a broad-spectrum plant growth regulator. It has the characteristics and physiological functions of endogenous auxin indole acetic acid, such as promoting cell division and expansion, inducing the formation of adventitious roots, increasing fruit setting, and preventing flower and fruit drop.

Physical and chemical properties: the pure product is white crystal, the industrial product is yellowish brown, insoluble in water. It is often processed into soluble sodium salt or potassium salt, and then used as a solution. Its detailed introduction is described in detail in the 323rd to 324th pages of the "Chemical Product Handbook" Agrochemicals. This book was printed for the 3rd time in September, 2000 by the Chemical Industry Press, edited by Sha Jiajun.

Zeatin

The zeatin described in the present invention is also a kind of cytokinin, which belongs to growth regulating substance, can promote cell division and cell enlargement, and break seed dormancy. Its chemical formula is: C ₁₀ H ₁₃ N ₅ O, its molecular weight is 219.25, and its structural formula is:

Features: A cytokinin, and a rare component in ribonucleic acid (RNA). Off-white or yellow powder, melting point is 207-208°C. Zeatin can be purchased from the market.

Carbendazim:

English name: Carbendazin; MBC; Bavistin(BASF); Delsene(Du Pont)

Molecular formula: C ₉ H ₉ N ₃ O ₂ , molecular weight: 191.12,

Its structural formula:

Chemical name: methyl benzimidazol-2-ylcarbamate

Physical and chemical properties: The pure product is white crystalline powder, sublimation begins at 216-217°C, melting point is 307-312°C, specific gravity is about 1.45, almost insoluble in water, soluble in inorganic acids and organic acids such as acetic acid, and forms corresponding salts.

Uses: Carbendazim belongs to the class of benzimidazoles. It is a broad-spectrum systemic fungicide with high efficiency and low toxicity. It can control wheat scab, rice blast, cotton blight, anthracnose, fusarium wilt, and rape sclerotia Disease, sugarcane black spot, sugar beet brown spot, peanut leaf spot, melon powdery mildew, rot, apple black spot, pear black spot, etc. Its detailed introduction is on pages 195-197 of the book "Handbook of Chemical Products" (Agrochemicals Third Edition). This book was printed for the third time in September 2000 by the Chemical Industry Press, edited by Sha Jiajun.

The preparation method of tamarisk root water extract among the present invention:

Adding liquid nitrogen to fresh tamarisk root to freeze-dry and grind it into powder, then immersing in water for 24 hours, and then filtering, the obtained filtrate is freeze-dried by a freeze dryer, and the obtained residue is the water extract of tamarisk root.

The preparation method of Haloxylon root water extract among the present invention:

The fresh Haloxylon root was freeze-dried and pulverized by adding liquid nitrogen, then soaked in water for 24 hours, and then filtered, and the obtained filtrate was freeze-dried by a freeze dryer, and the obtained residue was the aqueous extract of Haloxylon root.

In the present invention, the preparation method of the root water extract of Radix japonica:

Add liquid nitrogen to freeze-dry the fresh root of Nitraria japonicus, then immerse in water for 24 hours, and then filter, the obtained filtrate is freeze-dried with a freeze dryer, and the obtained residue is the water extract of the root of Nitraria japonicus.

The selected components of the seed treatment agent of the present invention, such as gibberellin, naphthaleneacetic acid, zeatin and carbendazim, are all known commercial products. The extraction methods of several root water extracts are also relatively simple.

The preparation method of the present invention is also a conventional method, that is, adding each component into a container according to a certain proportion, then adding water to the required capacity and then stirring evenly.

The method of using the seed treatment agent of the present invention: directly immerse the seeds of the Cistanche plant in the solution of the seed treatment agent, and after a period of time, the seeds can be sown after the seeds begin to show white.

The application of the invention can increase the germination rate of Cistanche tuberosa seeds to more than 75%, reduce the amount of seeds by more than 10 times, and can greatly increase the inoculation rate of Cistanche tuberoses.

The seed treatment agent containing different plant root water extracts of the present invention is suitable for different species in the genus Cistanche:

The seed treatment agent containing tamarisk root water extract of the present invention is mainly used for Cistanche tuberosa.

The seed treatment agent containing Haloxylon root water extract of the present invention is mainly used for Cistanche deserticola.

The seed treatment agent containing the root water extract of the white thorn root of the present invention is mainly used for growing Cistanche saline.

The advantages and beneficial effects of the present invention are: (1) the present invention greatly improves the germination rate of Cistanche seeds, the germination rate of Cistanche seeds treated by the present invention can reach more than 75%; (2) the present invention greatly reduces the amount of seeds used and can reduce Seed consumption is more than 10 times, can greatly improve the inoculation rate of Cistanche tubulosa simultaneously; (3) production method of the present invention is relatively simple wherein gibberellin, naphthalene acetic acid, zeatin and carbendazim all can go to the market and buy, tamarisk root water The extraction method of extract, Haloxylon root water extract, and Nitraria root water extract is simple, and then each component can be mixed evenly; (4) the present invention has low toxicity to humans and animals, and is environmentally friendly, and the composition of the present invention except a small amount of In addition to the hormone, it is the plant root water extract, so it is non-toxic or low-toxic; (5) the method of the present invention is simple and easy to popularize.

Detailed ways

The source of raw materials used in the following examples:

Gibberellin: Beijing Chemical Reagent Company;

Naphthaleneacetic acid: Beijing Chemical Reagent Company;

Zeatin: Beijing Chemical Reagent Company;

Carbendazim: Lianyungang Yabang Jintun Pesticide Co., Ltd.

Sterile water: Distilled water is made by autoclaving.

Preparation of tamarisk root water extract: Fresh tamarix root was added with liquid nitrogen freeze-dried ground powder, soaked in water for 24 hours, filtered, and the filtrate was freeze-dried by a freeze dryer, and the obtained residue was the water extract of tamarisk root.

Preparation of water extract of Haloxylon root: Fresh Haloxylon root added with liquid nitrogen freeze-dried ground powder soaked in water for 24 hours, filtered, and the filtrate was freeze-dried by a freeze dryer, and the obtained residue was Haloxylon root water extract.

The preparation of the water extract of the root of the white thorn: the fresh root of the white thorn is added with liquid nitrogen and freeze-dried and ground into water for 24 hours, filtered, and the filtrate is freeze-dried by a freeze dryer, and the residue obtained is the water extract of the root of the white thorn.

Preparation Example 1

Add 0.001 g of Tamarix root water extract, 0.0001 g of gibberellin, 0.0001 g of naphthalene acetic acid, 0.0001 g of zeatin, and 0.1 g of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare a seed treatment Agent 1.

Preparation Example 2

Add 0.5 grams of Tamarix root water extract, 0.001 grams of gibberellin, 0.01 grams of naphthalene acetic acid, 0.01 grams of zeatin, and 1 gram of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare a seed treatment Agent 2.

Preparation Example 3

Add 2 grams of Tamarix root water extract, 0.01 grams of gibberellin, 0.1 grams of naphthaleneacetic acid, 0.1 grams of zeatin, and 2 grams of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare seed treatment Agent 3.

Preparation Example 4

Add 0.1g of Tamarix root water extract, 0.0001g of gibberellin, 0.01g of naphthaleneacetic acid, 0.01g of zeatin, and 1g of carbendazim, add water to 100ml, mix, homogenize in a homogenizer, and prepare a seed treatment Agent 4.

Preparation Example 5

Add 0.001 g of Haloxylon root water extract, 0.0001 g of gibberellin, 0.0001 g of naphthalene acetic acid, 0.0001 g of zeatin, and 0.1 g of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare seeds Treatment agent 5.

Preparation Example 6

Add 0.5 grams of Haloxylon root water extract, 0.001 grams of gibberellin, 0.01 grams of naphthaleneacetic acid, 0.01 grams of zeatin, and 1 gram of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare seeds Treatment agent 6.

Preparation Example 7

Add 2 grams of Haloxylon root water extract, 0.01 grams of gibberellin, 0.1 grams of naphthalene acetic acid, 0.1 grams of zeatin, and 2 grams of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare seeds Treatment agent 7.

Preparation Example 8

Add 0.1 g of Haloxylon root water extract, 0.0001 g of gibberellin, 0.001 g of naphthaleneacetic acid, 0.01 g of zeatin, and 1 g of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare seeds Treatment agent 8.

Preparation Example 9

Add 0.001 g of water extract of the root of the white thorn, 0.0001 g of gibberellin, 0.0001 g of naphthalene acetic acid, 0.0001 g of zeatin, and 0.1 g of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare seeds Treatment agent 9.

Preparation Example 10

Add 0.5 g of water extract of Radix nitrabilis root, 0.001 g of gibberellin, 0.01 g of naphthaleneacetic acid, 0.01 g of zeatin, and 1 g of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare seeds Treatment agent 10.

Preparation Example 11

Add water to 100 ml, mix 2 grams of water extract of the root of Nitraria radix, 0.01 grams of gibberellin, 0.1 grams of naphthaleneacetic acid, 0.1 grams of zeatin, and 2 grams of carbendazim, mix them, homogenize them in a homogenizer, and prepare them into seeds Treatment agent 11.

Preparation Example 12

Add 0.1 g of water extract of the root of Nitraria japonica, 0.0001 g of gibberellin, 0.001 g of naphthaleneacetic acid, 0.01 g of zeatin, and 1 g of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare seeds Treatment agent 12.

Preparation Example 13

Add 0.0001 g of gibberellin, 0.001 g of naphthaleneacetic acid, 0.01 g of zeatin and 1 g of carbendazim to 100 ml of water, mix, homogenize in a homogenizer, and prepare seed treatment agent 13.

Preparation Example 14

Add 0.001 g of gibberellin, 0.01 g of naphthaleneacetic acid, 0.0001 g of zeatin and 1 g of carbendazim to 100 ml of water, mix, homogenize in a homogenizer, and prepare seed treatment agent 14.

Preparation Example 15

Add 0.01 g of gibberellin, 0.1 g of naphthalene acetic acid, 0.001 g of zeatin and 1 g of carbendazim to 100 ml of water, mix, homogenize in a homogenizer, and prepare seed treatment agent 15.

Preparation Example 16

Add 0.0001 g of gibberellin, 0.001 g of naphthalene acetic acid, 0.1 g of zeatin and 1 g of carbendazim, add water to 100 ml, mix, homogenize in a homogenizer, and prepare seed treatment agent 16.

Experimental Example 1

Disinfect 100 Cistanche cistanche seeds with 75% alcohol for 0.5 minutes, and then sterilize with 1% sodium hypochlorite for 15 minutes. Air dry and set aside. Take another petri dish with a diameter of 90 cm, put 5 layers of filter paper in the dish, add 10 grams of seed treatment agent 1, or seed treatment agent 2, or seed treatment agent 3, or seed treatment agent 4, and treat it with water as a blank control. Put the sterilized seeds evenly on the filter paper, seal it with a parafilm, put it in a 25°C cultivation room for cultivation, and count the germination rate after two weeks. Four replicates were set up for each treatment (results are shown in Table 1).

Experimental example 2

Disinfect 100 Cistanche seeds with 75% alcohol for 0.5 minutes, and then sterilize with 1% sodium hypochlorite for 15 minutes. Air dry and set aside. Another petri dish with a diameter of 90 cm was taken, and 5 layers of filter paper were placed in the dish, and 10 grams of seed treatment agent 5, or seed treatment agent 6, or seed treatment agent 7, or seed treatment agent 8 were added, and treated with water as a blank control. Put the sterilized seeds evenly on the filter paper, seal it with a parafilm, put it in a 25°C cultivation room for cultivation, and count the germination rate after two weeks. Four replicates were set up for each treatment (results are shown in Table 1).

Experimental Example 3

Disinfect 100 raw Cistanche seeds with 75% alcohol for 0.5 minutes, and then sterilize with 1% sodium hypochlorite for 15 minutes. Air dry and set aside. Get another petri dish with a diameter of 90 cm, put 5 layers of filter paper in the dish, add 10 grams of seed treatment agent 9, or seed treatment agent 10, or seed treatment agent 11, or seed treatment agent 12, and treat it with water as a blank control. Put the sterilized seeds evenly on the filter paper, seal it with a parafilm, put it in a 25°C cultivation room for cultivation, and count the germination rate after two weeks. Four replicates were set up for each treatment (results are shown in Table 1).

Table 1 Seed treatment machine treatment Cistanche seed germination test results

Note: germination rate = number of germinated seeds/total number of tested seeds * 100%

The result shows (see table 1), the seed treatment agent that contains different plant root water extracts that preparation example 1-12 of the present invention prepares can significantly promote Cistanche plant seed germination, preparation embodiment 13-16 preparation contains gibberellin , naphthalene acetic acid, zeatin, and carbendazim can effectively promote the germination of Cistanche seeds, while the seeds treated with water (blank control) did not germinate, indicating that water treatment has basically no effect.

Claims (5)

1. a seed treatment agent for promoting the germination of cistanche seeds, its composition and weight percentage are: Gibberellin 0.0001%～0.1%; Naphthaleneacetic acid 0.0001%～0.1%; Zeatin 0.0001%～0.1%; Carbendazim 0.1% to 2%; Tamarix root water extract, or Haloxylon root water extract, or Nitraria root water extract 0.001% to 2%; The rest is water. 2. according to the described seed treatment agent of claim 1, it is characterized in that its composition and percentage by weight are: Gibberellin 0.0001%～0.1%; Naphthaleneacetic acid 0.0001～0.1%; Zeatin 0.0001%～0.1%; Carbendazim 0.1% to 2%; Tamarix root water extract 0.001% to 2%; The rest is water. 3. according to the described seed treatment agent of claim 1, it is characterized in that its composition and percentage by weight are: Gibberellin 0.0001%～0.1%; Naphthaleneacetic acid 0.0001%～0.1%; Zeatin 0.0001%～0.1%; Carbendazim 0.1% to 2%; Haloxylon root water extract 0.001% ~ 2%; The rest is water. 4. according to the described seed treatment agent of claim 1, it is characterized in that its composition and percentage by weight are: Gibberellin 0.0001～0.1%; Naphthaleneacetic acid 0.0001%～0.1%; Zeatin 0.0001%～0.1%; Carbendazim 0.1% to 2%; Water extract of the root of the white thorn 0.001% ~ 2%; The rest is water. 5. The application of the seed treatment agent described in any one of claims 1 to 4 on the germination of succulents.