CA3207970A1

CA3207970A1 - Herbicidal composition containing carotenoid synthesis inhibitor and use thereof

Info

Publication number: CA3207970A1
Application number: CA3207970A
Authority: CA
Inventors: Feng Qiu; Yong Yin; Xiaojuan LUO; Shuangyang LI; Xiaowei Liu; Ling LIANG
Original assignee: Individual
Current assignee: Lier Cropscience Co Ltd
Priority date: 2021-02-10
Filing date: 2022-02-07
Publication date: 2022-08-18
Also published as: CN114903052A; WO2022171061A1; BR112023016011A2

Abstract

Disclosed are a herbicidal composition containing a carotenoid synthesis inhibitor and the use thereof. The herbicidal composition comprises herbicidally active components, wherein the herbicidally active components comprise an active component A, an active component B and an active component C, and the active component A is atrazine or ametryn, the active component B is topramezone, and the active component C is glufosinate or a salt thereof, and the mass ratio of the active components A, B and C is (2-16):(0.05-3.2):(15-40).

Description

HERBICIDAL COMPOSITION CONTAINING CAROTENOID SYNTHESIS
INHIBITOR AND USE THEREOF
Technical Field The present application relates to the field of pesticides, in particular to a herbicidal composition comprising a carotenoid synthesis inhibitor and the use thereof.
Background Art China is a vast country with a large area of widely distributed non-cultivated lands and orchards. There are numerous types of weeds, which are seriously harmful. At present, various types of herbicides are widely used in agricultural weed control.
However, due to the great differences in the environments, the regional dosage levels and application habits across the country, there are still many difficulties in weed control in non-cultivated lands and orchards. Herbicides are the main means for weed control in modern agriculture. At present, herbicides comprising a plurality of herbicidal ingredients have been developed. With the use of existing herbicides in large areas, many weeds have developed resistance to the existing commercially available herbicides, resulting in a decrease in the efficacies of the herbicides.
According to the report of the International Herbicide Resistance Action Committee (HRAC), glyphosate-resistant Erigeron canadensis appeared as early as 2006, and glyphosate-resistant Eleusine indica appeared in 2010. Therefore, continued use of existing herbicides not only aggravates pesticide resistance, making it impossible to control weeds effectively, but also aggravates environmental pollution.
On the other hand, non-cultivated lands and orchards distribute throughout all regions in the country, which are quite different in environment. In many regions, there is a demand for weeding in early spring before cultivation and in late autumn after cultivation. However, the common commercially available herbicides for non-cultivated lands and orchards are greatly affected by temperature and humidity, and their efficacies are extremely reduced at low temperatures or under drought conditions, making it difficult to work effectively. Moreover, weed control in non-cultivated lands and orchards is mainly targeted at elder weeds, which are difficult to control, especially elder gramineous weeds, which seriously affect the weed control effect in non-cultivated lands and orchards.
Technical Problem The present application provides a herbicidal composition, which has an excellent effect on comprehensive control of resistant weeds and elder weeds under special environmental conditions such as low temperatures or drought, and can be widely used for weed control in special environments such as non-cultivated lands and orchards.
Technical Solution The present application provides a herbicidal composition comprising herbicidally active ingredients, wherein the herbicidally active ingredients comprise atrazine or ametryn (active ingredient A), topramezone (active ingredient B), and glufosinate or a salt thereof (active ingredient C), wherein in the herbicidal composition, the mass ratio of the three ingredients, namely, atrazine or ametryn, to topramezone, to glufosinate or a salt thereof, is (2-16):(0.05-3.2):(15-40).
According to the present application, the herbicidally active ingredients consist of three species, namely, atrazine or ametryn (active ingredient A), topramezone (active ingredient B), and glufosinate or a salt thereof (active ingredient C).
According to the present application, in the herbicidal composition, the mass ratio of atrazine or ametryn, topramezone, and glufosinate or a salt thereof is (3-12):(0.075-

2.5):(20-35). According to some embodiments, the mass ratio of atrazine or ametryn, topramezone, and glufosinate or a salt thereof is (4-9):(0.1-1.5):(25-30).
According to the present application, the mass percentage of the herbicidally active ingredients is 10%-80%, based on the total mass of the herbicidal composition.
In some embodiments, the mass percentage of the herbicidally active ingredients is 30%-60%, based on the total mass of the herbicidal composition.
According to the present application, the herbicidal composition further comprises formulants for pesticides, for example, the auxiliary material can be any one or more of solvents, emulsifiers, dispersants, stabilizers, antifreeze agents and wetting agents.
According to the present application, the herbicidal composition is any one of aqueous suspensions, oil suspensions, wettable powders and water dispersible granules.
The present application further provides a use of the herbicidal composition to control weeds in non-cultivated lands or orchards. The weeds can be annual or perennial weeds.
In some embodiments, the herbicidal composition is applied in an amount of 300-g(a.i.)/ha.
According to the present application, the herbicidal composition is applied at a soil ambient humidity of 30%-85%, such as 30%-40%, 55%-65%, or 80%-85%.

According to the present application, the herbicidal composition is applied at an ambient temperature of 10 C-30 C, such as 10 C-15 C, or 25 C-30 C.
Advantageous Effects The herbicidal composition according to the present application exhibits significantly synergistic effects by combining herbicidal ingredients used for controlling low-aged weeds in a crop field with glufosinate in a specific ratio. The present herbicidal composition is capable of effectively controlling various malignant weeds and elder weeds commonly found in non-cultivated lands and orchards. In addition, the present composition can effectively overcome the influences of external environments such as low temperatures and drought, maintain good weed control effects, significantly broaden the weed control spectrum, significantly reduce the dosage of each active ingredient, and can be widely applied. In addition, the combination of topramezone, ametryn and glufosinate shows a more excellent effect, and can effectively control elder weeds and resistant weeds in a non-crop field, such as various elder grass and broadleaf weeds at 10 leaf-stage or more, and can also effectively control annual and perennial persistent weeds.
Description of Drawings Fig. 1 shows target damage symptoms and growth inhibition in the indoor determination of activity against resistant Eleusine indica in Test Example 3, 30 days after treating the resistant Eleusine indica with Comparative Examples and herbicidally active ingredient single formulations respectively, from left to right, blank control (water), Comparative Example G13, Comparative Example G14, Comparative Example G15, topramezone single formulation 60 g(a.i.)/ha, atrazine single formulation 1200 g(a.i.)/ha, and glufosinate single formulation 600 g(a.i.)/ha.
Fig. 2 shows target damage symptoms and growth inhibition in the indoor determination of activity against resistant Eleusine indica in Test Example 3, 30 days after treating the resistant Eleusine indica, from left to right, Comparative Example Gl, Comparative Example G2, Comparative Example G7, Comparative Example G4, Comparative Example G5, Comparative Example G6, Comparative Example G10, and Comparative Example G11, respectively.
Fig. 3 shows target damage symptoms and growth inhibition in the indoor determination of activity against resistant Eleusine indica in Test Example 3, 30 days after treating the resistant Eleusine indica, from left to right, Example G15, Example G16, Example G17, Example G3, Example G5, Example G7, Example G9, and Example G11, respectively.

3 Fig. 4 shows target damage symptoms and growth inhibition in the indoor determination of activity against resistant Eleusine indica in Test Example 3, 30 days after treating the resistant Eleusine indica, from left to right, Example G1, Example G13, Example G14, Example G18, Example G20, Example G22, Example G24, and Example G26, respectively.
Embodiments of the Invention The specific embodiments of the present application are described in detail hereinafter.
The present application provides a herbicidal composition comprising herbicidally active ingredients, wherein the herbicidally active ingredients comprise a photosystem II inhibitor (active ingredient A), a carotenoid synthesis inhibitor (active ingredient B), and glufosinate or a salt thereof (active ingredient C), wherein in the herbicidal composition, the photosystem II inhibitor (active ingredient A), the carotenoid synthesis inhibitor (active ingredient B), and glufosinate or a salt thereof (active ingredient C).
Glufosinate or a salt thereof is a non-conductive contact herbicide. It controls weeds by inhibition of glutamine synthetase, leads to nitrogen metabolism disorder in plants and toxicity due to nitrogen accumulation, and in turn hinders chloroplast synthesis and photosynthesis, eventually leading to the death of the plants. Glufosinate is characterized by low toxicity, high safety, broad weed control spectrum, high activity, fast weed control and the like. Moisture, temperature and illumination have great influence on the activity of glufosinate. At low temperatures (at or below 15 C), glufosinate is less effective. The efficacy of glufosinate is reduced when the soil moisture is very low. In the present application, glufosinate or a salt thereof can be all stereoisomers of glufosinate and mixtures thereof Specific examples of glufosinate or a salt thereof include glufosinate in the narrow sense, i.e., D,L-2-amino-4-[hydroxy(methyl)phosphinyl]butyric acid, and can be racemate of D-glufosinate and L-glufosinate, or can be L-glufosinate, wherein L-glufosinate has herbicidally activity.
Examples of the salt of glufosinate include at least one of glufosinate ammonium salt, glufosinate sodium salt, glufosinate potassium salt, glufosinate monoethanolamine salt or glufosinate dimethylamine salt, and the like. In some embodiments, glufosinate and the salt thereof may be commercially available, under the tradenames such as BastaTM
and LibertyTM. In some embodiments, the glufosinate is racemate of D-glufosinate and L-glufosinate, wherein the mass percentage of L-glufosinate is 50% of the glufosinate.
In the herbicidal composition according to the present application, the mass of active ingredient A is on the basis of the mass of L-glufosinate.

4 The photosystem II inhibitor is selected from at least one of atrazine and ametryn.
Ametryn, a selective systemic conductive herbicide, is a typical photosynthesis inhibitor. It is known that it can be used to control annual weeds in crop fields for growing sugarcane, corn, soybean, potato, pea, carrot and the like. However, when ametryn is used alone, the spraying effect is usually better when the soil is wet, and its efficacy will be reduced when the soil is dry.
Atrazine is a systemic selective pre-emergence and post-emergence herbicide.
This herbicide is mainly absorbed by roots, with little absorption by stems and leaves. It is quickly delivered to meristems and leaves of plants, interfering with photosynthesis and killing weeds. It is generally used to control broadleaf weeds and gramineous weeds in crop fields for growing corn, sorghum, sugarcane and the like, and its efficacy is seriously affected by drought and rainfall.
In the herbicidal composition according to the present application, the carotenoid synthesis inhibitor can be topramezone.
Topramezone, whose chemical name is [3-(4,5-dihydro-3-isoxazoly1)-4-methylsulfony1-2-methylbenzene]5-hydroxy-1-methyl-1H-pyrazol-4-yl)methanone, is a broad-spectrum post-emergence herbicide known in the field, which can effectively control annual gramineous weeds and broadleaf weeds in cornfields. However, topramezone has poor weed control effect on elder weeds when applied alone. In the present application, topramezone compound known in the field can be selected as a herbicidally active ingredient of the present application, and it can be in various crystal forms or morphologies, as long as it has herbicidally activity.
In addition to the herbicidally active ingredients, the herbicidal composition according to the present may further comprise additional active ingredients and/or formulants for pesticides. In some embodiments, the mass percentage of the herbicidally active ingredients is 6%-85%, based on the total mass of the herbicidal composition.
In some embodiments, the mass percentage of the herbicidally active ingredients is 30%-60%, based on the total mass of the herbicidal composition. The herbicidal composition may be in the form of any one of aqueous suspensions, oil suspensions, wettable powders, water dispersible granules and microcapsule-suspended suspension.
In some embodiments, the active ingredient A in the herbicidal composition is atrazine or ametryn, and the active ingredient B is topramezone. The mass ratio of active ingredient A, active ingredient B and active ingredient C is (2-16): (0.05-3.2):(15-40), for example, the mass ratio is (3-12):(0.075-2.5):(20-35), and as an example, the mass ratio is (4-9):(0.1-1.5):(25-30). In some embodiments, the herbicidal composition is wettable powders or water dispersible granules. Based on the total mass of the herbicidal composition, the mass percentage of the herbicidally active ingredients is 11%-79%, such as 11%-70%, 31%-71%, and 31%-59%. In other embodiments, the herbicidal composition is an aqueous suspension or an oil suspension. The mass percentage of the herbicidally active ingredients is 11%-55%, such as 31%-47%, or 31%-39%, based on the total mass of the herbicidal composition.
In some embodiments, the herbicidal composition according to the present application comprises herbicidal active ingredients, the herbicidal active ingredients comprising ametryn (ingredient A), topramezone (ingredient B), and glufosinate or a salt thereof (ingredient C), co-formulated in a specific ratio. In some embodiments, the mass ratio of ingredients A, B and C is (2-16): (0.05-3.2): (15-40). In other embodiments, the mass ratio of ingredients A, B and C is (3-12): (0.075-2.5): (15-35), for example, (3-12):
(0.075-2.5): (20-35), (4-9): (0.1-1.5): (25-30), or (2-4): (0.05-0.1): (15-25). In other embodiments, the mass ratio of ingredients A, B and C is (2-4): (0.075-0.1):
15, (2-4):
(0.075-0.1): 20, or (3-5): (0.05-0.1): 25. In other embodiments, the mass ratio of ingredients A, B and C is 5: (0.1-1.2): (25-30).
In the present application, the mass ratio of ingredients A, B and C
represents the mass ratio of the three ingredients. The range of the corresponding ratios includes all mass ratios that fall within the ratio range of the present application after being multiplied or divided by any coefficient. For example, 90:1:320 divided by a coefficient of 8 equals 11.25: 0.125: 40, which falls within the range of (2-16): (0.05-3.2): (15-40).
Not limited to the listed examples, other mass ratios of the three ingredients fall within the protection scope of the present application, as long as the mass ratio obtained by multiplying or dividing said other mass ratio by any coefficient is within the ratio range of the present application. In addition, those skilled in the field would understand that it is also acceptable to the present application if a certain mass ratio falls within the maximum mass ratio range, and the range of the mass ratio obtained by dividing the maximum mass ratio by a coefficient also falls within a smaller mass ratio range. For example, 8:3.2:40 falls within the range of (2-16): (0.05-3.2): (15-40). The mass ratio 8:3.2:40 reduced by 2 times proportionally (that is 4:1.6:20) also falls within the range of (3-12): (0.075-2.5): (15-35) recited in the present application.
The herbicidal composition provided in the present application can be used to control weed. Therefore, the present application also provides a use of the herbicidal composition in weed control; or further provides a method for controlling weeds. In some embodiments, the use or the method for controlling weeds refers to applying the herbicidal composition to non-cultivated lands or orchards, so that it contacts with weeds. Of course, those skilled in the art would understand that the herbicidal composition according to the present application can also be applied to other possible environments other than non-cultivated lands or orchards, such as woodlands and the like. In the present application, orchards, also called fruit orchard, refer to fields for planting fruit trees, including but not limited to the field for planting citrus, apple, pear, peach, plum, prune, apricot and the like. Non-cultivated lands refer to areas where no crops are planted, including but not limited to areas in front of and behind houses;
railways, highways, warehouses, forest fire prevention facility, ditchsides, roadsides and the like.
The herbicidal composition according to the present application is effectively applied in an amount of 300-900 g(a.i.)/ha, for example 350-800 g(a.i.)/ha, 400-700 g(a.i.)/ha, or 750 g(a.i.)/ha. In some embodiments, the herbicidal composition can be applied by application means known in the field. For example, the herbicidal composition is added to a solvent to formulate a spray solution and sprayed against weeds. In some embodiments, the herbicidal composition is sprayed on the stems and leaves of weeds.
The herbicidal composition according to the present application is applied at a soil humidity of 30%-85%, such as 30%-40%, 55%-65%, or 80%-85%. In some embodiments, the herbicidal composition still maintains good herbicidal effect especially under drought conditions, for example, at an ambient humidity of 30%-40%.
The herbicidal composition can be applied at an ambient temperature of 10 C-30 C, such as 10 C-15 C, or 25 C-30 C. In some embodiments, the herbicidal composition still maintains good herbicidal effect especially under low temperature conditions, for example, at an ambient temperature of 10 C-15 C.
In the present application, "herbicidally active ingredient" includes substances with the following functions: after contacting with undesired or unwanted weeds, such substances can inhibit or prevent the germination or growth of seeds of such weeds, or inhibit or prevent the continued growth of weeds that have already grown, or destroy the growth and metabolism pathways of weeds, so that they cannot continue to grow or even die. In some embodiments, the weeds targeted by the herbicidal composition according to the present application comprise gramineous weeds and broadleaf weeds, including annual or perennial weeds. In some specific embodiments, the weeds targeted by the herbicidal composition include, but are not limited to, Agropyron mongolicum, Dactyloctenium aegyptium, Panicum repens, Agrostis clavata, Brachiaria eruciformis, Leptochloa fusca, Leymus mollis, Cleistogenes serotina, Agropyron cristatum, Zizania latifolia, Chloris virgata, Zoysia japonica, Physochlaina physaloides, Elsholtzia densa, Ammannia multiflora, Nertera sinensis, Scleromitrion verticillatum, Lithospermum erythrorhizon, Glechoma longituba, Cynanchum chinense, Trigonella foenum-graecum, Saussurea amara, Lycopus lucidus, Thlaspi arvense, Persicaria orientalis, Elymus kamoji, Paspalum distichum, Reynoutria japonica, Carduus crispus, Aegilops tauschii, Fagopyrum tataricum, Puccinellia distans, Miscanthus sacchariflorus, Euphorbia heterophylla, Heteropappus hispidus, Eleusine indica, Zinnia elegans, Geranium carolinianum or Pseudosclerochloa kengiana. In some embodiments, the weeds targeted by the herbicidal composition are resistant weeds, such as glufosinate-resistant weeds, glyphosate-resistant weeds, such as resistant Eleusine indica.
The technical solutions of the present application will be clearly and completely described below in conjunction with the examples of the present application.
Apparently, the described examples are merely some of the examples of the present application, other than all of them.
The formulants for the wettable powders in the Examples and Comparative Examples of the present application include: naphthalene sulfonates, sodium lauryl sulfate, lignossulfonates, silicon dioxide, and kaolin.
The formulants for the aqueous suspensions in the Examples and Comparative Examples of the present application include: lignosulfonates, sodium lauryl sulfate, xanthan gum, ethylene glycol, organosilicones, and water.
The formulants for the oil suspensions in the Examples and Comparative Examples of the present application include: vegetable oils and derivatives thereof (methyl oleate), calcium dodecylbenzenesulfonate, castor oil polyoxyethylene ether, polycarboxylates, alkylphenol polyoxyethylene ether, and organic bentonite.
The types of formulants used in the same formulation type in each of the Examples and Comparative Examples of the present application are the same, and the ratios of the formulants are also the same.
The active ingredients of the herbicidal compositions of the Examples and Comparative Examples of the present application are shown in Table 1-1 and Table 1-2.
Table 1-1 List of the Ingredients in the Herbicidal Compositions of Examples 1-and Comparative Examples 1-14 Formulation type and active Other active mass p active ingredient A active ingredient B ingredient ingredient ercentage No. C
of active ingredients Atrazine Ametryn Topramezone Tembotrione lsooxadiazon Glufosinate 54%
Example 1 2 0.05 15 wettable powder 70%
Example 2 2.5 3.2 15 wettable powder 35%
Example 3 16 0.05 15 aqueous suspension 33%
Example 4 15 3.2 15 aqueous suspension 38%
Example 5 2 0.05 40 aqueous suspension 43%
Example 6 2 3.2 38 wettable powder 36%
Example 7 16 0.05 40 aqueous suspension 56%
Example 8 16 3.2 39 wettable powder 43%
Example 9 12 2.5 35 wettable powder 37%
Example 10 12 1.2 22 aqueous suspension 55%
Example 11 3 0.075 20 wettable powder 67%
Example 12 5 0.7 25 wettable powder Example 13 6 1 25 31%

aqueous suspension 38%
Example 14 5 1.2 25 wettable powder 52%
Example 15 5 0.3 30 wettable powder 55%
Example 16 5.5 0.7 30 wettable powder 49%
Example 17 7 1 30 wettable powder 33%
Example 18 9 1.5 30 aqueous suspension 26%
Example 19 2 0.05 15 aqueous suspension 18%
Example 20 2.5 3.2 15 aqueous suspension 37%
Example 21 16 0.05 15 aqueous suspension 58%
Example 22 15 3.2 15 wettable powder 46%
Example 23 2 0.05 40 wettable powder 38%
Example 24 2 3.2 38 aqueous suspension 39%
Example 25 16 0.05 40 aqueous suspension 33%
Example 26 16 3.2 39 aqueous suspension 50%
Example 27 12 2.5 35 wettable powder 35%
Example 28 12 1.2 22 wettable powder 35%
Example 29 4 0.3 25 aqueous suspension 43%
Example 30 5 0.7 25 wettable powder 49%
Example 31 6 1 25 wettable powder 31%
Example 32 5 1.2 26 aqueous suspension 61%
Example 33 5 0.3 30 wettable powder 42%
Example 34 5.5 0.7 30 wettable powder 38%
Example 35 7 1 30 aqueous suspension 72%
Example 36 9 1.5 30 wettable powder 39%
Comparative 20 3.5 10 aqueous Example 1 suspension 50%
Comparative 1 0.03 50 wettable Example 2 powder 44%
Comparative 20 3.5 10 wettable Example 3 powder 62%
Comparative 1 0.03 50 wettable Example 4 powder 42%
Comparative Pyroxasulfone wettable Example 5 8 powder Comparative Pyroxasulfone 42%
6.2 1.5 25 Example 6 8 wettable powder 33%
Comparative 0.7 25 Prometryn

5 aqueous Example 7 suspension 35%
Comparative 25 Mesotrione 0.7 wettable Example 8 powder Mesotrione 37%
Comparative 0.7+Prometryn aqueous Example 9 suspension 38%
Comparative aqueous Example 10 suspension 40%
Comparative aqueous Example 11 suspension 33%
Comparative aqueous Example 12 suspension Comparative 42% oil Example 13 suspension Comparative 38% oil Example 14 suspension Table 1-2 List of the Ingredients in the Herbicidal Compositions of Examples Gl-G29 and Comparative Examples Gl-G15 Ingredient C (parts Ingredient A Ingredient B (parts Other active No. by weight) (parts by weight) by weight) Formulation type ingredient Glufosinate Ametryn Topramezone 0.1 / 35%
aqueous Example G1 30 4 suspension 8.6 / 55%
aqueous Example G2 40.5 5.4 suspension Example G3 37.57 40 0.13 /
77% wettable powder Example G4 30 32 6.4 /
68% wettable powder 0.1 / 56%
aqueous Example G5 50 4 suspension 4.5 / 48%
aqueous Example G6 39.3 3.1 suspension Example G7 42.5 27.2 0.1 /
70% wettable powder Example G8 45 28.8 5.8 /
58% wettable powder 0.05 / 40%
aqueous Example G9 40 2.05 suspension 2.2 / 32%
aqueous Example G10 27.6 1.4 suspension Example G11 40 16 0.05 / 58%
wettable powder Example G12 40 16 3.2 / 59%
wettable powder 0.2 / 35%
aqueous Example G13 40 6 suspension 0.2 / 33%
aqueous Example G14 8.4 2.5 suspension Example G15 20 9 0.6 / 41%
wettable powder Example G16 20 12 1.5 / 38%
wettable powder 0.8 / 44%
aqueous Example G17 15 6 suspension 0.5 / 40%
aqueous Example G18 31.6 7.1 suspension Example G19 50 10 0.6 / 57%
wettable powder Example G20 25 5 0.5 / 46%
wettable powder 0.7 / 32%
aqueous Example G21 25 5 suspension 1.8 / 39%
aqueous Example G22 44.5 8.9 suspension Example G23 25 5 1.2 / 58%
wettable powder Example G24 30 5 0.3 / 40%
wettable powder 0.5 / 36%
aqueous Example G25 30 5 suspension 0.9 / 45%
aqueous Example G26 39 6.5 suspension Example G27 30 5 1 / 36%
wettable powder Example G28 42 7 1.7 / 52%
wettable powder Example G29 10 1.7 0.5 / 50%
wettable powder Comparative 3.5 / 36%
aqueous Example G1 suspension Comparative 0.03 / 31%
aqueous Example G2 suspension Comparative 1 / 35%
aqueous Example G3 suspension Comparative 0.03 / 52%
wettable powder Example G4 Comparative 3.5 / 54%
wettable powder Example G5 Comparative 0.03 / 63%
wettable powder Example G6 Comparative Pyroxasulfone 25 6 1 40%
wettable powder Example G7 8 Comparative 54%
aqueous 50 / 1 Atrazine 3.5 Example G8 suspension Comparative 33%
aqueous 25 / 0.7 Atrazine 5 Example G9 suspension Comparative 25 / 0.7 Prometryn 5 35% wettable powder Example G10 Atrazine Comparative 38%
aqueous 25 / / 5+Mesotrione Example G11 suspension 0.7 Prometryn Comparative 32%
aqueous 25 / / 5+Mesotrione Example G12 suspension 0.7 Comparative 31%
aqueous Example G13 suspension Comparative 35%
aqueous Example G14 suspension Comparative 50 15 / / 55%
wettable powder Example G15 Test Example 1 Test for Determining Toxicity of Herbicidal Compositions against Weeds Raw Materials for Test: 95% glufosinate (technical material); 97% topramezone (technical material); 98% atrazine (technical material); 98% ametryn (technical material).
Plant Materials for Test: Agropyron mongolicum, Dactyloctenium aegyptium, Panicum repens, Agrostis clavata, Brachiaria eruciformis, Leptochloa fusca, Leymus mollis, Cleisto genes serotina, Agropyron crista turn, Zizania latifolia, Chloris virgata, Zoysia japonica, Physochlaina physaloides, Elsholtzia densa, Ammannia multiflora, Nertera sinensis, Scleromitrion verticillatum, Lithospermum erythrorhizon, Glechoma longituba, Cynanchum chinense, Trigonella foenum, Saussurea amara, Lyco pus lucidus, and Thlaspi aryense. The seeds or underground bulbs or aboveground rhizome of these weeds are collected from the fields by the researchers from Lier Cropscience Co., Ltd., Sichuan, China, and stored in a storehouse for further use.
Preparation of Formulations: Each of the technical materials is weighted. As for topramezone, ametryn and atrazine, the mother liquor of individual single herbicide is formulated by adding topramezone, ametryn or atrazine to acetone and, after complete dissolution, diluting the resultant solution with a 0.1% aqueous Tween 80 solution. As for glufosinate, the mother liquor of single herbicide is formulated by directly adding glufosinate to a 0.1% aqueous Tween 80 solution. A series of ratios are set according to the compounding purpose. 8-13 concentration gradients are set for each ratio. The specific ratios are shown in Tables 2 to 13.
Control Solution: 0.1% aqueous Tween 80 solution Test Method: As used is the method for treating stems and leaves of a potted plant (NY/T 1155.4-2006): filling a specified amount of dried soil into a plastic pot, wherein the plastic pot has a height of 17 cm and a diameter of 20.5 cm. For Panicum repens, Zizania latifolia, Nertera sinensis, and Glechoma longituba, planting 15-20 rhizomes or ramets thereof in the plastic pot, and then covering them with 1-2 cm thick fine soil.
As for other target weeds, sowing 20-30 seeds of the target weed into the plastic pot, covering them with 0.5-1 cm thick fine soil; placing the plants in a greenhouse for cultivation; ensuring that the soil in each pot has a relative humidity of 75%
after sowing by absorbing water from the bottom; placing the plants in a greenhouse for cultivation, and supplementing water as desired according to the humidity of the soil so as to ensure that the weeds can grow normally. Other conditions are the same.
When weeds grow to 10-12 leaf-stage, they are transplanted in a density of 10 plants per pot for further use. After the relative humidity of the soil decreases to 40%, water is supplemented based on a daily measurement of the weight, and the humidity of the soil is maintained at 40%. Stems and leaves are then treated by spraying, and each treatment is repeated 4 times. A treatment without any herbicide is considered as a control.
Application Method: stem-leaf spray treatment. The spray applies Model 3WPSH-500D spray tower for bioassay (manufactured by Nanjing Agricultural Mechanization Research Institute of the Chinese Ministry of Agriculture) with a disc diameter of 50 cm, a rotation speed of the main shaft of 6 rpm, an aperture diameter of the nozzle of 0.3 mm, a spray pressure of 0.3 M Pa, a diameter of the sprayed droplet of 100 pm, and a nozzle flow rate of 90 mL/min. After treatment, the test materials are cultured in a greenhouse at a temperature of 20 C (during the day) to 15 C (at night) and an air humidity of 60-70%. The growth of the target weeds is observed regularly.
After 30 days, the damage symptoms and growth inhibition of the targets are visually observed, and the fresh weight of the aboveground parts are weighed. The survival rate of the target weed is used to evaluate the toxicity of the herbicide on the target weed.
Investigation Method: On day 30 after the treatment, the damage symptoms and growth inhibition of the targets are visually observed, the fresh weight of the aboveground parts are weighed, and the weed survival rate (%) are calculated.
According to the data obtained from the investigation, statistical analysis is carried out by Excel processing software.
fresh weight of weeds treated with herbicide weed survival rate (%) = X 100 fresh weight of weeds treated with blank control The combined effect is evaluated by Colby's method:
X * Y Z
Ea = .

wherein:
X is the weed survival rate for herbicide A when the dosage is P;
Y is the weed survival rate for herbicide B when the dosage is Q;
Z is the weed survival rate for herbicide C when the dosage is W;
Ea is the theoretical weed survival rate for herbicide (A+B+C) when the dosage is (P+Q+W);
E is the actual weed survival rate for herbicide (A+B+C) when the dosage is (P+Q+W);
if Eo-E > 10%, the combined effect is a synergistic effect; if Eo-E <-10%, the combined effect is an antagonistic effect; and if the value of Eo-E is between -10% and 10%, the combined effect is an additive effect.
Table 2. Determination of Indoor Toxicity of Compound of Glufosinate, Atrazine and Topramezone against Agropyron mongolicum and Physochlaina physaloides C+A+B Agropyron mongol/cum Physochlaina physaloides No. dosage Theoretical g(a=i=)/mu Actual Weed Theoretical Actual Weed Weed Survival Rate Weed Survival E.- E(%) Survival Rate Eo- E(%) Survival E(%) Rate E0(%) E(%) Rate E0(%)

6 90.24 91.47 9 84.63 86.46 12 75.21 77.57 15 64.26 65.46 C: Glufosinate 18 53.74 55.68 21 48.29 47.23 24 42.86 38.78 30 29.55 27.23 0.6 95.47 96.23 1.2 92.36 91.34 1.8 87.27 88.67 2.4 86.82 85.87 A: Atrazine 3 83.45 82.78 5.4 80.33 79.22

7.2 77.48 76.48 9.6 75.29 73.21 12 63.17 71.57 0.018 98.63 97.45 0.03 95.36 94.37 0.045 93.46 91.26 0.06 89.58 88.53 0.18 86.48 87.43 0.3 84.26 85.48 = B
0.42 80.74 81.51 Topramezone 0.6 78.53 77.87 0.72 76.24 75.38 0.9 74.52 72.92 1.5 65.39 66.47 1.92 53.78 54.38 2.1 43.86 48.22 1. 51.11 74.54 23.42 52.25 74.53 22.28 9+1.2+0.03 2. 9+1.2+0.045 49.53 73.05 23.53 49.42 72.07 22.65 3. 9+1.2+0.06 45.54 70.02 24.48 46.93 69.91 22.98 4. 9+1.2+0.9 38.80 58.25 19.44 39.53 57.59 18.06 5. 9+1.2+1.5 37.52 51.11 13.59 40.01 52.49 12.48 6. 9+1.2+1.92 28.48 42.04 13.55 30.35 42.95 12.60 7. 9+1.8+0.03 47.29 70.43 23.14 50.83 72.35 21.52

8. 9+1.8+0.045 45.48 69.03 23.55 47.30 69.96 22.66

9. 9+1.8+0.06 41.07 66.16 25.09 45.47 67.87 22.40

10. 9+1.8+0.9 35.98 55.04 19.06 38.33 55.90 17.58

11. 9+1.8+1.5 35.33 48.29

12.97 38.72 50.96 12.24 12. 9+1.8+1.92 26.38 39.72

13.34 29.70 41.69 11.99 13. 9+2.4+0.03 46.65 70.07 23.42 48.74 70.06 21.32

14. 9+2.4+0.045 46.67 68.67 22.00 44.73 67.75 23.02

15. 9+2.4+0.06 41.94 65.82 23.88 43.08 65.73 22.64

16. 9+2.4+0.9 35.56 54.75 19.20 37.53 54.14 16.61

17. 9+2.4+1.5 36.01 48.05 12.04 37.54 49.35 11.81

18. 9+2.4+1.92 26.42 39.52 13.09 28.72 40.37 11.65

19. 9+5.4+0.03 51.72 64.83 13.11 54.12 64.64 10.52

20. 9+5.4+0.045 44.74 63.54 18.79 45.64 62.51 16.87

21. 9+5.4+0.06 41.49 60.90 19.41 43.63 60.64 17.01

22. 9+5.4+0.9 32.21 50.66 18.45 32.43 49.95 17.52

23. 9+5.4+1.5 32.52 44.45 11.93 34.25 45.53 11.28

24. 9+5.4+1.92 23.96 36.56 12.60 23.83 37.25 13.42

25. 9+7.2+0.03 49.58 62.53 12.95 49.70 62.40 12.70

26. 9+7.2+0.045 50.01 61.28 11.27 46.66 60.35 13.68

27. 9+7.2+0.06 46.00 58.74 12.73 46.21 58.54 12.33

28. 9+7.2+0.9 36.14 48.86 12.72 35.46 48.22 12.76

29. 9+7.2+1.5 30.00 42.88 12.88 32.68 43.95 11.27

30. 9+7.2+1.92 20.97 35.26 14.30 24.42 35.96 11.54

31. 9+9.6+0.03 46.93 60.76 13.83 46.93 59.73 12.81

32. 9+9.6+0.045 45.79 59.55 13.76 47.22 57.77 10.55

33. 9+9.6+0.06 43.92 57.08 13.16 45.53 56.04 10.50

34. 9+9.6+0.9 34.11 47.48 13.38 33.30 46.16 12.85

35. 9+9.6+1.5 27.76 41.67 13.91 29.84 42.07 12.23

36. 9+9.6+1.92 20.64 34.27 13.62 20.84 34.42 13.58

37. 12+1.2+0.03 47.83 66.24 18.42 49.49 66.86 17.37

38. 12+1.2+0.045 47.28 64.92 17.64 47.01 64.66 17.65

39. 12+1.2+0.06 44.72 62.23 17.51 46.32 62.73 16.41

40. 12+1.2+0.9 31.73 51.76 20.04 34.26 51.67 17.41

41. 12+1.2+1.5 31.61 45.42 13.81 34.88 47.10 12.21

42. 12+1.2+1.92 23.01 37.36 14.34 24.23 38.53 14.29

43. 12+1.8+0.03 45.65 62.59 16.94 49.76 64.91 15.15

44. 12+1.8+0.045 37.55 61.34 23.79 40.27 62.77 22.50

45. 12+1.8+0.06 34.41 58.80 24.39 38.66 60.89 22.23

46. 12+1.8+0.9 29.62 48.91 19.29 33.49 50.16 16.67

47. 12+1.8+1.5 24.07 42.92 18.85 28.60 45.72 17.12

48. 12+1.8+1.92 21.59 35.30 13.71 25.04 37.40 12.36

49. 12+2.4+0.03 44.85 62.27 17.41 46.77 62.86 16.09

50. 12+2.4+0.045 36.56 61.03 24.46 39.17 60.79 21.62

51. 12+2.4+0.06 34.67 58.49 23.83 37.17 58.97 21.80

52. 12+2.4+0.9 28.70 48.66 19.96 30.76 48.57 17.81

53. 12+2.4+1.5 23.09 42.70 19.61 27.82 44.28 16.46

54. 12+2.4+1.92 21.22 35.12 13.90 24.23 36.22 11.99

55. 12+5.4+0.03 43.94 57.61 13.67 45.60 57.99 12.40

56. 12+5.4+0.045 37.78 56.46 18.69 38.45 56.08 17.63

57. 12+5.4+0.06 36.01 54.12 18.11 36.80 54.40 17.60

58. 12+5.4+0.9 27.80 45.02 17.22 28.06 44.81 16.75

59. 12+5.4+1.5 21.88 39.51 17.62 23.58 40.85 17.27

60. 12+5.4+1.92 19.65 32.49 12.84 22.62 33.42 10.79

61. 12+7.2+0.03 41.98 55.57 13.59 44.21 55.99 11.78

62. 12+7.2+0.045 35.05 54.46 19.41 37.09 54.14 17.05

63. 12+7.2+0.06 32.83 52.20 19.37 34.61 52.52 17.91

64. 12+7.2+0.9 24.07 43.42 19.36 24.73 43.26 18.53

65. 12+7.2+1.5 18.33 38.10 19.77 22.18 39.43 17.25

66. 12+7.2+1.92 18.26 31.34 13.08 21.13 32.26 11.13

67. 12+9.6+0.03 41.97 54.00 12.03 41.26 53.59 12.33

68. 12+9.6+0.045 40.01 52.92 12.92 40.01 51.83 11.81

69. 12+9.6+0.06 38.10 50.73 12.63 39.48 50.28 10.79

70. 12+9.6+0.9 29.35 42.20 12.85 29.32 41.41 12.09

71. 12+9.6+1.5 22.81 37.03 14.22 25.22 37.75 12.53

72. 12+9.6+1.92 17.30 30.45 13.15 19.46 30.88 11.42

73. 15+1.2+0.03 42.99 56.60 13.61 44.26 56.42 12.17

74. 15+1.2+0.045 42.62 55.47 12.85 43.53 54.57 11.04

75. 15+1.2+0.06 40.30 53.17 12.87 42.18 52.93 10.75

76. 15+1.2+0.9 30.91 44.23 13.32 30.80 43.60 12.80

77. 15+1.2+1.5 24.95 38.81 13.85 28.56 39.74 11.18

78. 15+1.2+1.92 17.29 31.92 14.63 20.49 32.51 12.02

79. 15+1.8+0.03 39.44 53.48 14.04 42.84 54.78 11.94

80. 15+1.8+0.045 33.38 52.41 19.03 34.83 52.97 18.14

81. 15+1.8+0.06 31.40 50.24 18.83 33.20 51.39 18.19

82. 15+1.8+0.9 22.54 41.79 19.25 23.93 42.33 18.39

83. 15+1.8+1.5 16.35 36.67 20.32 20.83 38.58 17.75

84. 15+1.8+1.92 16.51 30.16 13.65 19.82 31.56 11.74

85. 15+2.4+0.03 40.50 53.20 12.70 42.08 53.05 10.96

86. 15+2.4+0.045 33.25 52.14 18.89 34.50 51.30 16.80

87. 15+2.4+0.06 25.86 49.98 24.12 28.26 49.76 21.50

88. 15+2.4+0.9 18.40 41.58 23.18 18.71 40.99 22.28

89. 15+2.4+1.5 17.51 36.48 18.97 20.54 37.36 16.83

90. 15+2.4+1.92 16.64 30.00 13.36 19.81 30.57 10.76

91. 15+5.4+0.03 35.64 49.22 13.59 38.62 48.94 10.32

92. 15+5.4+0.045 28.03 48.24 20.21 29.46 47.33 17.86

93. 15+5.4+0.06 22.07 46.24 24.17 24.08 45.91 21.83

94. 15+5.4+0.9 13.67 38.47 24.80 15.56 37.81 22.25

95. 15+5.4+1.5 14.20 33.75 19.56 17.60 34.47 16.87

96. 15+5.4+1.92 14.42 27.76 13.34 16.68 28.20 11.52

97. 15+7.2+0.03 34.25 47.48 13.23 35.34 47.25 11.90

98. 15+7.2+0.045 27.39 46.53 19.14 28.67 45.69 17.02

99. 15+7.2+0.06 25.96 44.60 18.64 26.97 44.32 17.35

100. 15+7.2+0.9 18.19 37.10 18.91 19.59 36.51 16.92

101. 15+7.2+1.5 14.26 32.56 18.29 17.16 33.28 16.12

102. 15+7.2+1.92 13.24 26.78 13.53 15.02 27.22 12.20

103. 15+9.6+0.03 33.19 46.14 12.95 33.95 45.23 11.27

104. 15+9.6+0.045 32.45 45.22 12.77 31.60 43.73 12.13

105. 15+9.6+0.06 30.56 43.34 12.78 31.25 42.43 11.18

106. 15+9.6+0.9 23.47 36.05 12.58 23.89 34.95 11.06

107. 15+9.6+1.5 19.43 31.64 12.20 20.26 31.85 11.60

108. 15+9.6+1.92 11.87 26.02 14.15 14.03 26.06 12.03

109. 18+1.2+0.03 34.09 47.33 13.24 37.10 47.99 10.90

110. 18+1.2+0.045 32.12 46.39 14.27 34.30 46.41 12.11

111. 18+1.2+0.06 31.37 44.46 13.10 33.36 45.02 11.66

112. 18+1.2+0.9 22.79 36.99 14.20 25.20 37.09 11.89

113. 18+1.2+1.5 18.22 32.46 14.24 21.67 33.81 12.14

114. 18+1.2+1.92 12.98 26.69 13.71 16.95 27.66 10.70

115. 18+1.8+0.03 30.47 44.72 14.25 34.96 46.59 11.64

116. 18+1.8+0.045 24.00 43.83 19.83 26.69 45.06 18.37

117. 18+1.8+0.06 22.42 42.01 19.60 27.11 43.71 16.60

118. 18+1.8+0.9 16.07 34.95 18.88 18.45 36.00 17.55

119. 18+1.8+1.5 11.74 30.67 18.93 15.11 32.82 17.71

120. 18+1.8+1.92 13.40 25.22 11.82 15.92 26.85 10.93

121. 18+2.4+0.03 31.88 44.49 12.61 34.22 45.12 10.90

122. 18+2.4+0.045 24.67 43.61 18.94 26.65 43.63 16.99

123. 18+2.4+0.06 17.50 41.80 24.29 20.19 42.33 22.13

124. 18+2.4+0.9 11.38 34.77 23.39 13.06 34.86 21.81

125. 18+2.4+1.5 10.94 30.51 19.57 13.11 31.78 18.67

126. 18+2.4+1.92 5.42 25.09 19.67 7.85 26.00 18.16

127. 18+5.4+0.03 27.84 41.17 13.33 29.05 41.63 12.58

128. 18+5.4+0.045 21.24 40.35 19.11 22.81 40.25 17.44

129. 18+5.4+0.06 13.91 38.67 24.76 16.11 39.05 22.94

130. 18+5.4+0.9 8.52 32.17 23.65 10.56 32.16 21.60

131. 18+5.4+1.5 9.70 28.23 18.53 11.98 29.32 17.34

132. 18+5.4+1.92 4.00 23.22 19.21 6.97 23.99 17.01

133. 18+7.2+0.03 26.43 39.71 13.27 29.67 40.19 10.52

134. 18+7.2+0.045 18.87 38.91 20.05 20.96 38.86 17.90

135. 18+7.2+0.06 17.42 37.30 19.88 19.48 37.70 18.22

136. 18+7.2+0.9 11.36 31.03 19.67 14.03 31.05 17.02

137. 18+7.2+1.5 8.04 27.23 19.19 11.12 28.31 17.19

138. 18+7.2+1.92 3.82 22.39 18.57 6.00 23.16 17.16

139. 18+9.6+0.03 25.38 38.58 13.20 28.04 38.47 10.43

140. 18+9.6+0.045 24.93 37.81 12.89 26.27 37.20 10.93

141. 18+9.6+0.06 17.28 36.24 18.97 19.11 36.09 16.98

142. 18+9.6+0.9 11.17 30.15 18.99 12.83 29.72 16.89

143. 18+9.6+1.5 7.87 26.46 18.58 9.21 27.10 17.89

144. 18+9.6+1.92 2.61 21.76 19.15 5.14 22.17 17.03

145. 21+1.2+0.03 28.89 42.53 13.64 29.28 40.71 11.43

146. 21+1.2+0.045 28.26 41.68 13.43 27.39 39.37 11.98

147. 21+1.2+0.06 27.03 39.95 12.92 25.96 38.19 12.23

148. 21+1.2+0.9 19.92 33.24 13.32 19.50 31.46 11.96

149. 21+1.2+1.5 15.64 29.16 13.52 15.68 28.68 12.99

150. 21+1.2+1.92 10.27 23.99 13.71 11.30 23.46 12.16

151. 21+1.8+0.03 26.56 40.19 13.63 27.80 39.52 11.72

152. 21+1.8+0.045 20.72 39.39 18.66 22.61 38.22 15.61

153. 21+1.8+0.06 18.43 37.75 19.32 20.33 37.08 16.74

154. 21+1.8+0.9 12.22 31.40 19.18 13.69 30.54 16.85

155. 21+1.8+1.5 7.68 27.56 19.88 9.12 27.84 18.72

156. 21+1.8+1.92 8.74 22.66 13.92 10.14 22.77 12.64

157. 21+2.4+0.03 25.30 39.98 14.68 26.64 38.27 11.63

158. 21+2.4+0.045 21.39 39.18 17.80 20.90 37.01 16.11

159. 21+2.4+0.06 18.50 37.56 19.06 19.12 35.90 16.78

160. 21+2.4+0.9 12.25 31.24 18.99 13.37 29.57 16.20

161. 21+2.4+1.5 7.38 27.42 20.04 8.79 26.96 18.17

162. 21+2.4+1.92 3.04 22.55 19.51 4.18 22.05 17.87

163. 21+5.4+0.03 23.45 36.99 13.54 24.01 35.31 11.30

164. 21+5.4+0.045 17.86 36.25 18.39 17.58 34.15 16.56

165. 21+5.4+0.06 15.38 34.75 19.37 15.72 33.12 17.40

166. 21+5.4+0.9 4.69 28.91 24.21 5.74 27.28 21.54

167. 21+5.4+1.5 5.89 25.37 19.47 8.14 24.87 16.73

168. 21+5.4+1.92 2.45 20.86 18.41 3.53 20.35 16.81

169. 21+7.2+0.03 21.48 35.68 14.20 22.15 34.09 11.94

170. 21+7.2+0.045 15.39 34.97 19.58 14.84 32.96 18.12

171. 21+7.2+0.06 13.80 33.52 19.72 13.30 31.98 18.67

172. 21+7.2+0.9 3.16 27.88 24.72 4.39 26.34 21.95

173. 21+7.2+1.5 4.52 24.47 19.95 6.61 24.01 17.40

174. 21+7.2+1.92 0.71 20.12 19.42 2.27 19.64 17.37

175. 21+9.6+0.03 21.61 34.67 13.06 20.40 32.63 12.23

176. 21+9.6+0.045 20.25 33.98 13.73 19.74 31.56 11.82

177. 21+9.6+0.06 13.33 32.57 19.24 14.13 30.61 16.48

178. 21+9.6+0.9 7.45 27.09 19.64 8.55 25.21 16.67

179. 21+9.6+1.5 5.09 23.77 18.69 6.08 22.98 16.90

180. 21+9.6+1.92 1.63 19.55 17.93 1.79 18.80 17.01

181. 24+1.2+0.03 23.87 37.75 13.88 21.57 33.43 11.86

182. 24+1.2+0.045 23.98 37.00 13.01 21.12 32.33 11.20

183. 24+1.2+0.06 22.29 35.46 13.17 19.69 31.36 11.67

184. 24+1.2+0.9 16.53 29.50 12.96 13.66 25.83 12.17

185. 24+1.2+1.5 13.13 25.88 12.76 12.66 23.54 10.89

186. 24+1.2+1.92 8.61 21.29 12.68 7.69 19.26 11.57

187. 24+1.8+0.03 23.68 35.67 11.98 20.08 32.45 12.37

188. 24+1.8+0.045 22.57 34.96 12.39 20.24 31.38 11.15

189. 24+1.8+0.06 20.60 33.51 12.91 18.51 30.44 11.94

190. 24+1.8+0.9 14.52 27.87 13.36 12.13 25.07 12.95

191. 24+1.8+1.5 10.48 24.46 13.98 10.77 22.86 12.09

192. 24+1.8+1.92 7.24 20.12 12.87 7.59 18.70 11.11

193. 24+2.4+0.03 22.54 35.48 12.94 19.71 31.43 11.72

194. 24+2.4+0.045 21.70 34.78 13.07 20.11 30.39 10.28

195. 24+2.4+0.06 13.97 33.33 19.37 11.75 29.48 17.73

196. 24+2.4+0.9 8.71 27.73 19.02 6.34 24.28 17.94

197. 24+2.4+1.5 6.48 24.33 17.85 6.02 22.13 16.11

198. 24+2.4+1.92 1.89 20.01 18.12 2.12 18.11 15.99

199. 24+5.4+0.03 18.91 32.83 13.93 17.57 28.99 11.42

200. 24+5.4+0.045 19.38 32.18 12.80 16.48 28.04 11.56

201. 24+5.4+0.06 12.58 30.84 18.27 11.25 27.20 15.94

202. 24+5.4+0.9 1.57 25.66 24.09 0.38 22.40 22.02

203. 24+5.4+1.5 3.68 22.51 18.83 3.71 20.42 16.71

204. 24+5.4+1.92 0.24 18.52 18.28 0.25 16.71 16.46

205. 24+7.2+0.03 19.28 31.67 12.39 16.96 27.99 11.03

206. 24+7.2+0.045 19.19 31.04 11.85 14.57 27.07 12.50

207. 24+7.2+0.06 12.07 29.75 17.68 10.11 26.26 16.14

208. 24+7.2+0.9 2.17 24.75 22.58 0.51 21.63 21.12

209. 24+7.2+1.5 2.64 21.71 19.08 2.09 19.71 17.62

210. 24+7.2+1.92 0.76 17.86 17.10 0.77 16.13 15.36

211. 24+9.6+0.03 19.47 30.77 11.30 14.52 26.79 12.27

212. 24+9.6+0.045 17.82 30.16 12.33 14.81 25.91 11.10

213. 24+9.6+0.06 11.21 28.91 17.70 8.70 25.13 16.43

214. 24+9.6+0.9 6.38 24.05 17.66 4.88 20.70 15.82

215. 24+9.6+1.5 1.83 21.10 19.27 1.75 18.87 17.12

216. 24+9.6+1.92 0.68 17.35 16.67 0.33 15.44 15.11

217. 6+12+2.1 16.07 25.00 8.93 24.79 31.57 6.77

218. 6+12+0.018 52.04 56.22 4.18 56.43 63.80 7.37

219. 30+0.6+0.018 19.18 27.82 8.65 17.72 25.54 7.81

220. 30+0.6+2.1 4.23 12.37 8.14 5.12 12.64 7.52

221. 30+12+0.018 9.46 18.41 8.95 10.89 18.99 8.11

222. 15+3+0.18 22.28 46.37 24.09 23.85 47.38 23.53

223. 15+3+0.3 21.28 45.18 23.91 23.51 46.32 22.81

224. 15+3+0.42 18.61 43.30 24.68 20.70 44.17 23.47

225. 15+3+0.6 18.16 42.11 23.95 18.14 42.20 24.06

226. 15+3+0.72 17.63 40.88 23.26 17.90 40.85 22.95

227. 18+3+0.18 16.62 38.78 22.16 16.20 40.30 24.10

228. 18+3+0.3 12.78 37.79 25.01 14.95 39.40 24.45

229. 18+3+0.42 11.48 36.21 24.73 13.73 37.57 23.84

230. 18+3+0.6 12.13 35.22 23.09 13.34 35.89 22.55

231. 18+3+0.72 10.15 34.19 24.04 10.25 34.74 24.50 According to the results from the indoor toxicity test shown in Table 2, it can be seen that the herbicidal compositions comprising the three herbicidally active ingredients in a specific ratio provided by the present application show a good synergistic effect, in particular, show good effect against both Agropyron mongolicum and Physochlaina physaloides. It can be seen from the distribution of Eo-E(%) in Table 2 that, the combined effect produced by the compound of glufosinate, atrazine, and topramezone does not increase linearly, but shows synergistic or additive effects within the ranges of designed ratios in the test. Moreover, the applicant surprisingly found that, as compared with other ratios, when the mass ratio of glufosinate: atrazine:
topramezone is in the range of 15-40:2-16:0.05-3.2, the synergistic effect is significantly superior.
Referring to the test results of No. 217-221, it can be clearly seen that when the compounding ratio of the three active ingredients is not within the range of 15-40:2-16:0.05-3.2, there is merely an additive effect on Agropyron mongolicum and Physochlaina physaloides.
Table 3. Determination of Indoor Toxicity of Compound of Glufosinate, Ametryn and Topramezone against Dactyloctenium aegyptium and Elsholtzia densa No. C+A+B Dactyloctenhim aegypthim Elsholtzia densa Dosage g (a .i.)/mu Heoretica I
Actual Weed Heoretica I Actual Weed Weed Survival Rate Weed Survival E.- E(%) Survival Rate Eo- E(%) Survival Rate E(%) Rate E0(%) E(%) E0(%) C:G I uf osi na te 6 87.82 90.59 77.63 86.17 66.23 79.91 57.91 72.61 54.28 64.22 48.94 54.99 44.77 41.92 28.68 35.62 97.24 95.66 0.6 93.69 91.91 1.2 91.30 87.89 1.8 86.22 85.97 2.4 A:Ametryn 82.08 81.39 80.29 80.02 5.4 76.25 77.51 7.2 72.97 74.98 9.6 67.20 72.81 99.98 99.21 0.018 98.98 98.97 0.03 97.29 96.98 0.045 93.69 93.50 0.06 88.93 91.00 0.18 B:
81.43 88.53 Topramezone 0.3 76.91 81.70 0.42 74.70 77.70 0.6 71.41 71.52 0.72 68.4 68.78 0.9 60.29 64.88 1.5 54.35 57.97 1.92 39.87 46.78 2.1 1. 47.39 71.99 24.60 55.57 78.38 22.81 9+1.2+0.03 2. 47.83 70.76 22.93 54.33 76.81 22.48 9+1.2+0.045 3. 43.21 68.14 24.93 52.02 74.05 22.03 9+1.2+0.06 4. 32.13 49.75 17.62 36.23 54.47 18.24 9+1.2+0.9 5. 29.81 43.85 14.04 39.01 51.38 12.37 9+1.2+1.5 6. 25.42 39.53 14.11 33.26 45.91 12.65 9+1.2+1.92 7. 47.23 70.15 22.92 52.39 74.95 22.56 9+1.8+0.03 8. 44.51 68.96 24.45 49.94 73.45 23.51 9+1.8+0.045 9. 42.21 66.40 24.19 48.89 70.81 21.92 9+1.8+0.06 10. 29.20 48.48 19.28 33.67 52.09 18.42 9+1.8+0.9 11. 28.99 42.73 13.74 37.15 49.14 11.99 9+1.8+1.5 12. 24.36 38.52 14.16 31.29 43.90 12.61 9+1.8+1.92 13. 43.11 66.25 23.14 50.41 73.32 22.91 9+2.4+0.03 14. 42.81 65.12 22.31 49.72 71.84 22.12 9+2.4+0.045 15. 38.41 62.71 24.30 47.10 69.27 22.17 9+2.4+0.06 16. 26.16 45.78 19.62 32.35 50.95 18.60 9+2.4+0.9 17. 27.88 40.35 12.47 34.72 48.06 13.34 9+2.4+1.5 18. 22.74 36.38 13.64 29.87 42.94 13.07 9+2.4+1.92 19. 48.59 61.69 13.10 55.93 68.24 12.31 9+5.4+0.03 20. 43.00 60.64 17.64 48.53 66.87 18.34 9+5.4+0.045 21. 41.14 58.40 17.26 46.15 64.47 18.32 9+5.4+0.06 22. 25.23 42.63 17.40 29.92 47.43 17.51 9+5.4+0.9 23. 25.24 37.58 12.34 32.49 44.74 12.25 9+5.4+1.5 24. 21.44 33.88 12.44 27.14 39.97 12.83 9+5.4+1.92 25. 46.05 58.59 12.54 52.39 66.10 13.71 9+7.2+0.03 26. 45.23 57.59 12.36 50.88 64.77 13.89 9+7.2+0.045 27. 42.83 55.46 12.63 48.21 62.45 14.24 9+7.2+0.06 28. 27.76 40.49 12.73 32.84 45.94 13.10 9+7.2+0.9 29. 23.27 35.69 12.42 30.18 43.33 13.15 9+7.2+1.5 30. 18.05 32.17 14.12 25.72 38.72 13.00 9+7.2+1.92 31. 42.29 56.07 13.78 52.22 63.94 11.72 9+9.6+0.03 32. 41.35 55.11 13.76 49.86 62.66 12.80 9+9.6+0.045 33. 40.23 53.07 12.84 47.97 60.41 12.44 9+9.6+0.06 34. 25.10 38.75 13.65 32.31 44.44 12.13 9+9.6+0.9 35. 20.74 34.15 13.41 30.21 41.92 11.71 9+9.6+1.5 36. 16.86 30.79 13.93 26.05 37.45 11.40 9+9.6+1.92 37. 43.95 61.42 17.47 55.04 72.69 17.65 12+1.2+0.03 38. 43.98 60.37 16.39 54.40 71.23 16.83 12+1.2+0.045 39. 40.68 58.14 17.46 52.83 68.67 15.84 12+1.2+0.06 40. 24.57 42.44 17.87 33.11 50.52 17.41 12+1.2+0.9 41. 22.57 37.41 14.84 34.30 47.65 13.35 12+1.2+1.5 42. 19.87 33.72 13.85 28.72 42.58 13.86 12+1.2+1.92 43. 42.71 59.85 17.14 52.51 69.51 17.00 12+1.8+0.03 44. 35.80 58.83 23.03 47.59 68.11 20.52 12+1.8+0.045 45. 32.80 56.65 23.85 44.29 65.67 21.38 12+1.8+0.06 46. 22.56 41.36 18.80 30.95 48.31 17.36 12+1.8+0.9 47. 17.12 36.46 19.34 28.85 45.57 16.72 12+1.8+1.5 48. 18.16 32.86 14.70 28.91 40.71 11.80 12+1.8+1.92 49. 37.92 56.52 18.60 50.89 67.99 17.10 12+2.4+0.03 50. 31.35 55.56 24.21 43.51 66.62 23.11 12+2.4+0.045 51. 29.11 53.50 24.39 41.50 64.23 22.73 12+2.4+0.06 52. 20.65 39.06 18.41 30.24 47.25 17.01 12+2.4+0.9 53. 15.75 34.43 18.68 27.89 44.57 16.68 12+2.4+1.5 54. 16.89 31.04 14.15 28.27 39.82 11.55 12+2.4+1.92 55. 39.42 52.63 13.21 51.01 63.29 12.28 12+5.4+0.03 56. 33.00 51.73 18.73 45.96 62.01 16.05 12+5.4+0.045 57. 31.37 49.82 18.45 43.39 59.79 16.40 12+5.4+0.06 58. 19.54 36.37 16.83 27.20 43.98 16.78 12+5.4+0.9 59. 13.85 32.06 18.21 24.32 41.49 17.17 12+5.4+1.5 60. 15.20 28.90 13.70 22.82 37.07 14.25 12+5.4+1.92 61. 36.04 49.99 13.95 48.31 61.30 12.99 12+7.2+0.03 62. 29.79 49.13 19.34 43.11 60.07 16.96 12+7.2+0.045 63. 28.34 47.31 18.97 40.10 57.91 17.81 12+7.2+0.06 64. 16.48 34.54 18.06 24.99 42.60 17.61 12+7.2+0.9 65. 11.50 30.45 18.95 22.28 40.19 17.91 12+7.2+1.5 66. 15.23 27.45 12.22 24.25 35.91 11.66 12+7.2+1.92 67. 36.06 47.84 11.78 46.91 59.30 12.39 12+9.6+0.03 68. 34.18 47.02 12.84 45.98 58.11 12.13 12+9.6+0.045 69. 32.60 45.28 12.68 42.78 56.02 13.24 12+9.6+0.06 70. 20.39 33.06 12.67 29.01 41.21 12.20 12+9.6+0.9 71. 14.83 29.14 14.31 26.40 38.87 12.47 12+9.6+1.5 72. 12.38 26.27 13.89 21.50 34.73 13.23 12+9.6+1.92 73. 40.05 53.70 13.65 52.83 66.05 13.22 15+1.2+0.03 74. 38.99 52.79 13.80 52.18 64.72 12.54 15+1.2+0.045 75. 37.33 50.83 13.50 49.27 62.40 13.13 15+1.2+0.06 76. 23.56 37.11 13.55 33.39 45.90 12.51 15+1.2+0.9 77. 18.95 32.71 13.76 31.22 43.30 12.08 15+1.2+1.5 78. 15.68 29.49 13.81 27.23 38.69 11.46 15+1.2+1.92 79. 38.16 52.33 14.17 50.07 63.16 13.09 15+1.8+0.03 80. 33.58 51.44 17.86 44.58 61.89 17.31 15+1.8+0.045 81. 31.83 49.54 17.71 43.28 59.67 16.39 15+1.8+0.06 82. 17.18 36.16 18.98 27.50 43.89 16.39 15+1.8+0.9 83. 13.50 31.88 18.38 24.71 41.40 16.69 15+1.8+1.5 84. 15.80 28.74 12.94 23.45 36.99 13.54 15+1.8+1.92 85. 36.23 49.42 13.19 48.79 61.78 12.99 15+2.4+0.03 86. 30.62 48.58 17.96 43.74 60.54 16.80 15+2.4+0.045 87. 22.38 46.78 24.40 36.73 58.37 21.64 15+2.4+0.06 88. 10.25 34.15 23.90 21.01 42.93 21.92 15+2.4+0.9 89. 11.65 30.10 18.45 22.81 40.50 17.69 15+2.4+1.5 90. 14.72 27.14 12.42 23.38 36.19 12.81 15+2.4+1.92 91. 32.64 46.02 13.38 45.01 57.50 12.49 15+5.4+0.03 92. 27.15 45.24 18.09 38.77 56.35 17.58 15+5.4+0.045 93. 19.54 43.56 24.02 31.72 54.33 22.61 15+5.4+0.06 94. 7.28 31.80 24.52 16.56 39.96 23.40 15+5.4+0.9 95. 8.36 28.03 19.67 20.77 37.70 16.93 15+5.4+1.5 96. 11.13 25.27 14.14 21.25 33.68 12.43 15+5.4+1.92 97. 29.71 43.71 14.00 43.54 55.70 12.16 15+7.2+0.03 98. 24.60 42.96 18.36 38.79 54.58 15.79 15+7.2+0.045 99. 22.98 41.37 18.39 35.22 52.62 17.40 15+7.2+0.06 100. 10.83 30.20 19.37 21.41 38.71 17.30 15+7.2+0.9 101. 8.39 26.62 18.23 18.88 36.51 17.63 15+7.2+1.5 102. 10.74 24.00 13.26 20.92 32.63 11.71 15+7.2+1.92 103. 28.15 41.83 13.68 42.13 53.88 11.75 15+9.6+0.03 104. 28.37 41.11 12.74 39.94 52.80 12.86 15+9.6+0.045 105. 26.65 39.59 12.94 37.75 50.90 13.15 15+9.6+0.06 106. 16.03 28.90 12.87 23.68 37.45 13.77 15+9.6+0.9 107. 12.91 25.48 12.57 21.94 35.32 13.38 15+9.6+1.5 108. 8.63 22.97 14.34 18.82 31.56 12.74 15+9.6+1.92 109. 36.43 50.34 13.91 45.69 58.42 12.73 18+1.2+0.03 110. 35.50 49.48 13.98 45.50 57.24 11.74 18+1.2+0.045 111. 34.28 47.65 13.37 42.71 55.19 12.48 18+1.2+0.06 112. 20.56 34.78 14.22 27.88 40.60 12.72 18+1.2+0.9 113. 16.65 30.66 14.01 26.26 38.30 12.04 18+1.2+1.5 114. 14.32 27.64 13.32 22.33 34.22 11.89 18+1.2+1.92 115. 35.10 49.05 13.95 44.76 55.86 11.10 18+1.8+0.03 116. 30.76 48.21 17.45 38.82 54.74 15.92 18+1.8+0.045 117. 27.74 46.43 18.69 36.59 52.77 16.18 18+1.8+0.06 118. 15.03 33.90 18.87 21.79 38.82 17.03 18+1.8+0.9 119. 10.93 29.88 18.95 19.49 36.62 17.13 18+1.8+1.5 120. 14.95 26.93 11.98 20.86 32.72 11.86 18+1.8+1.92 121. 33.92 46.32 12.40 42.15 54.64 12.49 18+2.4+0.03 122. 26.19 45.53 19.34 36.41 53.54 17.13 18+2.4+0.045 123. 19.33 43.85 24.52 28.98 51.62 22.64 18+2.4+0.06 124. 7.66 32.01 24.35 15.55 37.97 22.42 18+2.4+0.9 125. 11.37 28.22 16.85 19.01 35.82 16.81 18+2.4+1.5 126. 7.33 25.44 18.11 15.61 32.01 16.40 18+2.4+1.92 127. 28.87 43.14 14.27 38.21 50.86 12.65 18+5.4+0.03 128. 23.78 42.40 18.62 33.53 49.84 16.31 18+5.4+0.045 129. 16.08 40.83 24.75 26.01 48.05 22.04 18+5.4+0.06 130. 5.13 29.81 24.68 14.20 35.35 21.15 18+5.4+0.9 131. 7.83 26.28 18.45 15.46 33.34 17.88 18+5.4+1.5 132. 5.31 23.69 18.38 13.59 29.79 16.20 18+5.4+1.92 133. 27.75 40.97 13.22 36.51 49.26 12.75 18+7.2+0.03 134. 22.34 40.27 17.93 33.13 48.27 15.14 18+7.2+0.045 135. 20.45 38.78 18.33 30.29 46.54 16.25 18+7.2+0.06 136. 10.12 28.31 18.19 18.00 34.24 16.24 18+7.2+0.9 137. 6.57 24.95 18.38 14.98 32.30 17.32 18+7.2+1.5 138. 4.36 22.49 18.13 13.34 28.86 15.52 18+7.2+1.92 139. 25.83 39.20 13.37 34.98 47.66 12.68 18+9.6+0.03 140. 25.10 38.53 13.43 34.42 46.70 12.28 18+9.6+0.045 141. 18.95 37.11 18.16 29.34 45.02 15.68 18+9.6+0.06 142. 8.12 27.09 18.97 16.16 33.12 16.96 18+9.6+0.9 143. 6.79 23.88 17.09 14.39 31.24 16.85 18+9.6+1.5 144. 4.29 21.53 17.24 11.94 27.91 15.97 18+9.6+1.92 145. 32.40 45.38 12.98 36.94 50.02 13.08 21+1.2+0.03 146. 30.81 44.61 13.80 36.31 49.01 12.70 21+1.2+0.045 147. 29.68 42.96 13.28 33.62 47.26 13.64 21+1.2+0.06 148. 17.38 31.36 13.98 20.92 34.76 13.84 21+1.2+0.9 149. 13.74 27.64 13.90 18.46 32.79 14.33 21+1.2+1.5 150. 11.11 24.92 13.81 16.22 29.30 13.08 21+1.2+1.92 151. 29.75 44.23 14.48 33.96 47.83 13.87 21+1.8+0.03 152. 24.93 43.47 18.54 30.92 46.87 15.95 21+1.8+0.045 153. 23.47 41.86 18.39 27.52 45.19 17.67 21+1.8+0.06 154. 10.90 30.56 19.66 15.84 33.24 17.40 21+1.8+0.9 155. 9.09 26.94 17.85 15.38 31.36 15.98 21+1.8+1.5 156. 9.65 24.28 14.63 15.78 28.02 12.24 21+1.8+1.92 157. 27.71 41.77 14.06 34.28 46.79 12.51 21+2.4+0.03 158. 22.89 41.05 18.16 29.58 45.85 16.27 21+2.4+0.045 159. 21.71 39.53 17.82 27.66 44.20 16.54 21+2.4+0.06 160. 10.38 28.86 18.48 17.49 32.52 15.03 21+2.4+0.9 161. 7.94 25.44 17.50 13.13 30.67 17.54 21+2.4+1.5 162. 3.59 22.93 19.34 11.19 27.41 16.22 21+2.4+1.92 163. 24.93 38.89 13.96 31.63 43.55 11.92 21+5.4+0.03 164. 18.95 38.23 19.28 25.59 42.67 17.08 21+5.4+0.045 165. 18.01 36.81 18.80 25.32 41.14 15.82 21+5.4+0.06 166. 3.30 26.88 23.58 8.56 30.27 21.71 21+5.4+0.9 167. 5.29 23.69 18.40 12.55 28.55 16.00 21+5.4+1.5 168. 3.76 21.36 17.60 8.80 25.51 16.71 21+5.4+1.92 169. 22.98 36.94 13.96 29.28 42.18 12.90 21+7.2+0.03 170. 19.88 36.31 16.43 24.82 41.34 16.52 21+7.2+0.045 171. 17.26 34.96 17.70 23.05 39.85 16.80 21+7.2+0.06 172. 1.28 25.52 24.24 8.14 29.32 21.18 21+7.2+0.9 173. 4.17 22.50 18.33 10.58 27.65 17.07 21+7.2+1.5 174. 3.20 20.28 17.08 8.11 24.71 16.60 21+7.2+1.92 175. 21.48 35.35 13.87 27.42 40.81 13.39 21+9.6+0.03 176. 20.70 34.74 14.04 26.38 39.99 13.61 21+9.6+0.045 177. 15.10 33.46 18.36 21.91 38.55 16.64 21+9.6+0.06 178. 6.17 24.43 18.26 11.18 28.36 17.18 21+9.6+0.9 179. 3.92 21.53 17.61 10.21 26.75 16.54 21+9.6+1.5 180. 1.91 19.41 17.50 6.12 23.90 17.78 21+9.6+1.92 181. 27.31 41.52 14.21 25.41 38.13 12.72 24+1.2+0.03 182. 26.79 40.81 14.02 24.98 37.37 12.39 24+1.2+0.045 183. 25.69 39.30 13.61 22.34 36.02 13.68 24+1.2+0.06 184. 15.30 28.69 13.39 14.36 26.50 12.14 24+1.2+0.9 185. 11.98 25.29 13.31 12.47 25.00 12.53 24+1.2+1.5 186. 10.35 22.80 12.45 9.35 22.34 12.99 24+1.2+1.92 187. 28.35 40.46 12.11 23.53 36.46 12.93 24+1.8+0.03 188. 26.73 39.77 13.04 23.50 35.73 12.23 24+1.8+0.045 189. 25.25 38.30 13.05 20.78 34.45 13.67 24+1.8+0.06 190. 14.85 27.96 13.11 13.18 25.34 12.16 24+1.8+0.9 191. 11.25 24.64 13.39 11.10 23.90 12.80 24+1.8+1.5 192. 8.26 22.22 13.96 8.35 21.36 13.01 24+1.8+1.92 193. 24.78 38.21 13.43 22.94 35.67 12.73 24+2.4+0.03 194. 24.15 37.55 13.40 21.77 34.95 13.18 24+2.4+0.045 195. 17.64 36.16 18.52 17.18 33.70 16.52 24+2.4+0.06 196. 7.27 26.40 19.13 7.93 24.79 16.86 24+2.4+0.9 197. 4.77 23.27 18.50 6.76 23.38 16.62 24+2.4+1.5 198. 2.31 20.98 18.67 5.30 20.89 15.59 24+2.4+1.92 199. 21.23 35.58 14.35 20.66 33.20 12.54 24+5.4+0.03 200. 22.24 34.97 12.73 20.17 32.53 12.36 24+5.4+0.045 201. 14.46 33.68 19.22 14.94 31.36 16.42 24+5.4+0.06 202. 0.76 24.59 23.83 2.30 23.07 20.77 24+5.4+0.9 203. 3.64 21.67 18.03 6.06 21.76 15.70 24+5.4+1.5 204. 2.87 19.54 16.67 3.17 19.45 16.28 24+5.4+1.92 205. 21.02 33.79 12.77 18.51 32.16 13.65 24+7.2+0.03 206. 21.15 33.21 12.06 19.05 31.51 12.46 24+7.2+0.045 207. 13.05 31.98 18.93 14.10 30.38 16.28 24+7.2+0.06 208. 0.17 23.35 23.18 1.50 22.35 20.85 24+7.2+0.9 209. 2.56 20.58 18.02 5.09 21.08 15.99 24+7.2+1.5 210. 1.43 18.55 17.12 2.31 18.84 16.53 24+7.2+1.92 211. 19.70 32.34 12.64 17.27 31.11 13.84 24+9.6+0.03 212. 19.83 31.78 11.95 17.63 30.48 12.85 24+9.6+0.045 213. 13.89 30.61 16.72 12.11 29.39 17.28 24+9.6+0.06 214. 6.00 22.35 16.35 5.85 21.62 15.77 24+9.6+0.9 215. 2.76 19.70 16.94 4.45 20.39 15.94 24+9.6+1.5 216. 1.28 17.76 16.48 1.91 18.22 16.31 24+9.6+1.92 217. 15.60 23.53 7.93 21.13 30.86 9.73 6+12+2.1 218. 49.74 59.00 9.26 56.18 65.44 9.26 6+12+0.018 219 18.57 27.88 9.31 24.73 33.80 9.07 30+0.6+0.018 220. 4.52 11.12 6.60 8.64 15.94 7.30 30+0.6+2.1 221. 9.43 19.27 9.84 15.94 25.73 9.79 30+12+0.018 222. 19.16 42.27 23.11 29.69 53.78 24.09 15+3+0.18 223. 14.40 38.71 24.31 28.22 52.32 24.10 15+3+0.3 224. 13.00 36.56 23.56 22.99 48.28 25.29 15+3+0.42 225. 12.67 35.51 22.84 21.95 45.92 23.97 15+3+0.6 226. 10.01 33.94 23.93 18.04 42.27 24.23 15+3+0.72 227. 16.38 39.62 23.24 23.12 47.56 24.44 18+3+0.18 228. 11.85 36.28 24.43 22.76 46.27 23.51 18+3+0.3 229. 10.90 34.27 23.37 18.53 42.70 24.17 18+3+0.42 230. 10.19 33.28 23.09 17.32 40.61 23.29 18+3+0.6 231. 7.07 31.82 24.75 13.90 37.38 23.48 18+3+0.72 According to the results from the indoor toxicity test shown in Table 3, it can be seen that the herbicidal compositions comprising the three herbicidally active ingredients in a specific ratio provided by the present application show a good synergistic effect, and in particular show good effect on both Dactyloctenium aegyptium and Elsholtzia densa.
It can be seen from the distribution of Eo-E(%) in Table 3 that, the compounding herbicides of glufosinate, ametryn, and topramezone show synergistic or additive effect within the ranges of experimental design ratios. Moreover, the applicant surprisingly found that, as compared with other ratios, when the mass ratio of glufosinate:
ametryn:
topramezone is in the range of 15-40:2-16:0.05-3.2, the synergistic effect is significantly superior. Referring to the test results of No. 217-221 in Table 3, it can be clearly seen that when the compounding ratio of the three active ingredients is not within the range of 15-40:2-16:0.05-3.2, there is merely an additive effect on Dactyloctenium aegyptium and Elsholtzia densa.

Test Example 2 Efficacy Test 1. Efficacy Test in Non-Cultivated Field Test Method: The test is carried out in the wasteland of Zitong Country (J
iangyou, Sichuan Province, China). The application is conducted on a sunny day with a wind speed of 0.4 m/sec, a minimum temperature of 23.2 C, a maximum temperature of 34.4 C, and an air relative humidity of about 82%. There is no rain for 24 hours after the application. The temperature is 25 C-35 C and the soil relative humidity is 267%
during the observation after application. For each test, two test plots with uniform distribution of targets are selected, one plot for elder weeds and the other plot for small-aged weeds. No other herbicides are applied before and after the test. The weeds are stable in species and evenly distributed. Each treatment is repeated for 4 times. A region of 30 m2 is sampled from each small block. Upon application, the herbicidal composition is diluted with water to a spray volume of 40 L per mu, and a blank control is formulated by replacing the herbicidal composition with water. Randomized block permutation is applied. The equipment for spraying is a Model AGROLEX HD400 electric sprayer available from AGROLEX PTE Ltd. (Singapore). The spraying is conducted by the stem-leaf spray method. Other conditions for treating each region are completely identical except that the herbicides are applied according to the above scheme.
Main Targets: Elymus kamoji at 7-10 leaf-stage, Paspalum distichum with 3-5 branches, Reynoutria japonica at 8 leaf-stage, Carduus crispus at 5-7 leaf-stage, Elymus kamoji with 10-15 tillers, Paspalum distichum with 8- branches covering the ground, Reynoutria japonica with 40-50 cm height, and Carduus crispus with 50-cm height.
Evaluation Method: 35 days after spraying the herbicide, the weeds in the treated region are identified. 4 points are randomly sampled (the area of the sampling square is 1 m2) from each region during the investigation. The fresh weight of the aboveground parts of the target weeds within the sampling square is weighed, and the control efficacy on basis of fresh weight is calculated according to the following equation.
control efficacy on basis of fresh weight (%) fresh weight of weeds treated with blank control ¨ fresh weight of weeds treated with herbicide =
______________________________________________________________________________ fresh weight of weeds treated with blank control x 1.1 Field Efficacy Test of Herbicides (Glufosinate, Atrazine and Topramezone) Tested Herbicides: the ternary compounded herbicides of glufosinate, topramezone and atrazine in different ratios prepared according to Examples 1-18; and the binary or ternary compounded herbicides of glufosinate, topramezone and atrazine and the like in different combinations and ratios according to Comparative Example 1, Comparative Example 2, Comparative Example 6, Comparative Examples 7-9, Comparative Example 11, Comparative Example 12 and Comparative Example 14.
Control Herbicides:
200 g/L aqueous glufosinate solution (commercially available under the trade name of Besulton);
30% topramezone suspension (commercially available under the trade name of Baowei);
38% atrazine suspension (commercially available under the trade name of Guochang).
Table 4 Field Control Efficacy (%) of Different Compounded Compositions of Glufosinate, Atrazine and Topramezone, 35 Days after Application Small-aged Weeds Elder Weeds Paspalum Reynoutr Effective distichum Herbicide Elymus Paspalum Reynoutria Carduus Elymus ia Carduus Dosage with 8-12 Name kamoji at distichum japonica at crispus at kamoji japonica crispus with g(a.i.)/ha branches 7-10 leaf- with 3-5 8 leaf- 5-7 leaf-with 10-15 with 40- 50-100 cm covering stage branches stage stage tillers 50 cm height the height ground Glufosi nate 600 75.20 65.30 71.41 73.67 65.71 54.86 63.73 66.91 Atrazine 1200 62.56 64.62 62.24 61.34 47.33 42.23 47.28 46.08 Topramezone 60 52.81 53.5 63.09 64.63 35.13 39.37 48.34 42.24 Example 1 550 97.20 96.80 96.66 96.29 94.41 91.89 92.20 94.80 Example 2 550 91.05 91.00 91.81 91.62 87.18 85.11 86.05 88.00 Example 3 550 91.01 91.60 91.19 90.27 86.74 86.78 85.01 87.60 Example 4 550 90.85 89.95 91.60 91.30 85.60 85.51 85.85 85.95 Example 5 550 91.43 91.49 90.26 91.08 86.49 84.80 87.43 84.49 Example 6 550 89.96 91.32 89.27 91.66 86.92 85.48 85.96 85.32 Example 7 550 91.80 91.76 91.87 90.96 86.23 87.24 84.80 85.76 Example 8 550 93.42 94.87 94.70 94.16 89.86 89.10 90.42 90.67 Example 9 550 93.35 94.98 93.43 95.04 88.94 89.03 90.93 89.79 Example 10 550 93.93 94.19 92.86 93.95 89.63 90.14 89.77 90.25 Example 11 550 96.12 96.86 95.94 96.81 94.45 92.42 92.12 94.66 Example 12 550 96.77 96.27 98.23 97.98 92.06 92.28 92.77 93.27 Example 13 550 96.69 96.45 97.15 96.72 93.88 93.24 92.99 92.45 Example 14 550 96.46 96.59 95.88 96.34 93.42 92.60 92.46 93.59 Example 15 550 96.28 96.99 96.50 97.01 93.89 91.86 92.28 93.99 Example 16 550 96.39 95.85 96.17 96.78 93.75 92.32 92.39 92.85 Example 17 550 95.96 96.83 96.51 97.47 94.52 91.94 91.96 93.83 Example 18 550 97.03 96.07 97.74 95.86 94.93 93.47 93.03 92.07 Comparative 550 88.46 87.71 87.17 88.26 70.78 71.67 70.17 70.55 Example 1 Comparative 550 89.38 88.65 86.35 86.67 75.78 75.06 72.35 75.18 Example 2 Comparative 550 89.17 89.35 90.04 90.21 82.39 79.06 81.22 80.75 Example 6 Comparative 550 86.98 87.91 87.79 86.51 74.69 71.23 73.69 72.81 Example 7 Comparative 550 85.07 83.37 85.79 82.20 73.35 72.64 74.07 70.37 Example 8 Comparative 550 84.75 85.21 86.42 85.74 75.27 78.31 74.32 75.21 Example 9 Comparative 550 79.26 77.81 78.37 77.95 41.77 45.61 40.28 42.54 Example 11 Comparative 550 82.49 84.38 82.66 81.62 72.45 73.35 74.14 70.58 Example 12 Comparative 550 80.97 81.41 83.55 82.19 73.58 72.14 73.27 72.81 Example 14 It can be seen from the test results of the Examples and Comparative Examples as shown in Table 4 that, the ternary compounded herbicidal compositions provided by the Examples of the present application have good control efficacy against small-aged weeds and elder weeds in the environments such as non-cultivated lands, wherein the control efficacy on basis of fresh weight against elder weeds is obviously better than that of single formulations and binary compounds of individual active ingredients.
Moreover, it can be seen from the data comparison of the Examples and the Comparative Examples that, the herbicidal compositions with compounded ratios of the active ingredients provided by the present application have significantly better effects as compared with compositions with other compounded ratios in the Comparative Examples.
1.2 Field Efficacy Test of Herbicides (Glufosinate, Ametryn and Topramezone) Tested Herbicides: the ternary compounded herbicides of glufosinate, topramezone and ametryn in different ratios prepared according to Examples 19-36; and the binary or ternary compound herbicides of glufosinate, topramezone and ametryn and the like in different combinations and ratios according to Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 7, Comparative Example 9, Comparative Example 10, Comparative Example 12 and Comparative Example 13.

Control Herbicides:
200 g/L glufosinate aqueous solutions (commercially available under the trade name of Besulton);
30% topramezone suspension concentrate (commercially available under the trade name of Baowei);
80% ametryn wettable powder (commercially available under the trade name of J
inte).
Table 5 Field Control Efficacy (%) of Different Compounded Compositions of Glufosinate, Ametryn and Topramezone, 35 Days after Application Small-aged Weeds Large-aged Weeds Paspalum Effective Elymus Herbicide Paspalum Carduus Elymus distichum Reynoutria Carduus Dosage kamoji at Reynoutria Name distichum crispus at kamoji with 8-12 japonica crispus with g(a.i.)/ha 7-10 japonica at with 3-5 5-7 leaf- with 10-branches with 40-50 50-100 cm leaf- 8 leaf-stage branches stage 15 tillers covering cm height height stage the ground Glufosinate 600 75.87 66.04 71.53 74.00 66.40 55.86 63.85 67.82 Ametryn 1200 64.14 58.81 60.80 64.83 42.22 42.91 48.71 45.85 Topramezone 60 53.65 44.19 33.82 54.71 25.76 32.85 19.83 37.07 Example 19 550 98.87 99.73 98.11 98.16 96.17 94.31 94.37 96.73 Example 20 550 92.18 93.26 92.84 92.50 89.89 87.38 88.18 89.26 Example 21 550 93.17 93.04 93.36 91.93 88.87 88.13 87.17 89.04 Example 22 550 92.52 92.61 93.87 93.55 88.48 87.72 88.52 89.61 Example 23 550 93.86 93.11 93.49 93.19 87.94 88.27 89.86 88.11 Example 24 550 92.41 92.77 92.68 93.20 89.91 87.63 88.41 87.77 Example 25 550 93.06 93.17 93.09 92.53 88.13 88.85 89.06 88.17 Example 26 550 95.71 96.67 96.18 96.12 91.97 90.33 91.71 92.17 Example 27 550 95.53 96.53 95.69 96.30 92.11 90.70 92.71 91.54 Example 28 550 95.58 96.61 94.62 95.90 92.76 91.80 92.48 92.64 Example 29 550 98.82 98.82 98.34 98.42 96.87 94.98 94.82 96.02 Example 30 550 99.82 98.23 99.91 99.40 96.17 94.32 95.82 96.23 Example 31 550 98.88 98.72 98.81 98.58 96.44 95.22 94.88 95.72 Example 32 550 99.22 98.84 98.10 99.81 96.52 94.85 94.22 96.84 Example 33 550 97.83 98.39 98.56 99.19 96.05 93.73 93.83 95.39 Example 34 550 99.73 97.59 98.26 99.69 96.12 94.10 95.73 96.59 Example 35 550 97.79 98.78 98.25 99.16 95.90 93.16 93.79 95.78 Example 36 550 99.55 99.15 99.20 98.87 96.33 95.41 95.55 95.15 Comparative 550 91.12 91.43 90.85 91.42 72.75 71.08 72.15 73.63 Example 3 Comparative 550 90.12 89.35 91.04 90.68 71.80 72.16 75.35 74.47 Example 4 Comparative 550 91.09 90.07 91.21 92.85 88.55 87.73 86.09 88.07 Example 5 Comparative 550 86.98 87.91 87.79 86.51 74.69 71.23 73.69 72.81 Example 7 Comparative 550 85.39 85.43 86.88 86.59 74.88 77.66 78.39 75.43 Example 9 Comparative 550 80.26 81.96 80.11 77.25 41.49 41.02 45.17 42.96 Example 10 Comparative 550 82.77 84.05 82.54 81.85 71.77 72.82 74.81 70.67 Example 12 Comparative 550 83.55 80.19 83.19 81.20 74.05 73.23 72.21 73.51 Example 13 It can be seen from the test results of the Examples and Comparative Examples as shown in Table 5 that, all the ternary compounded herbicidal compositions provided by the Examples of the present application have good control efficacy against small-aged weeds such as Elymus kamoji at 7-10 leaf-stage, Paspalum distichum with 3-5 branches, Reynoutria japonica at 8 leaf-stage, Carduus crispus at 5-7 leaf-stage, and elder weeds such as Elymus kamoji with 10-15 tillers, Paspalum distichum with 8-12 branches covering the ground, Reynoutria japonica with 40-50 cm height, and Carduus crispus with 50-100 cm height in environments such as non-cultivated lands, wherein the control efficacy on basis of fresh weight against elder weeds is significantly better than that of single formulations and binary compounds of individual herbicidally active ingredients.
2. Effect of Temperature on Efficacy Main Targets: Aegilops tauschii with 10-15 tillers and Fagopyrum tataricum at leaf-stage.
Test Method: As used is the method for treating stems and leaves of an indoor potted plant. Filling a specified amount of soil into a plastic pot with a height of 10 cm and a diameter of 15 cm, and spot sowing specified amount of seeds of Aegilops tauschii and Fagopyrum tataricum in the pots respectively, 25-30 seeds in each pot;
cultivating in an artificial climate chamber, thinning out the seedlings after emergence, and merely retaining 10 plants in each pot; when the weeds grow to the desired stage for test, treating them by spraying using Model 3WP-2000 mobile spraying tower (each treatment is repeated for 8 times); after treatment, dividing the treated plants into two groups to continue cultivation, wherein one group is placed in an artificial climate chamber at 30 C (daytime) to 25 C (night), and the other group is placed in an artificial climate chamber at 15 C (daytime) to 10 C (night). The illumination intensity is 0 (night) to 7500 LX (daytime). The soil relative humidity for both test groups is 60-70%
during the test.
Evaluation Method: The control efficacy of each treatment against weeds is regularly observed. After 30 days, the fresh weight of the aboveground parts of weeds after each treatment is weighed, and the control efficacy of the herbicide on the target weed is calculated according to the following equation.
control efficacy on basis of fresh weight (%) fresh weight of weeds treated with blank control ¨ fresh weight of weeds treated with herbicide _______________________________________________________________________________ _ 100 fresh weight of weeds treated with blank control x 2.1 Effect of Temperature on Indoor Efficacy of Herbicides (Glufosinate, Atrazine and Topramezone) Tested Herbicides: the ternary compounded herbicides of glufosinate, topramezone and atrazine in different ratios prepared according to Examples 1-18; and the binary or ternary compounded herbicides of glufosinate, topramezone and atrazine and the like in different combinations and ratios according to Comparative Example 1, Comparative Example 2, Comparative Example 6, Comparative Examples 7-9, Comparative Example 11, Comparative Example 12 and Comparative Example 14.
Control Herbicides:
200 g/L glufosinate aqueous solutions (commercially available under the trade name of Besulton);
30% topramezone suspension concentrate (commercially available under the trade name of Baowei);
38% atrazine suspension concentrate (commercially available under the trade name of Guochang).
Table 6 Indoor Control Efficacy (%) of Different Compounded Compositions of Glufosinate, Atrazine and Topramezone, 30 Days after Application 30 C (daytime) to 25 C (night) 15 C (daytime) to 10 C (night) Effective Dosage Herbicide Name Fagopyrum Fagopyrum g(a.i.)/ha Aegllops tauschii Aegllops tauschll tataricum at 10 tataricum at 10 with 10-15 tillers with 10-15 tillers leaf-stage leaf-stage Glufosinate 600 79.58 82.1 60.95 63.39 Atrazine 1200 64.43 62.72 49.56 47.94 Topramezone 60 23.19 38.67 17.07 32.24 Example 1 550 96.45 96.27 94.44 92.74 Example 2 550 90.77 89.84 87.59 85.23 Example 3 550 90.11 90.71 88.27 84.12 Example 4 550 89.83 90.51 87.73 86.59 Example 5 550 89.22 90.61 85.64 85.68 Example 6 550 89.98 89.08 87.14 85.43 Example 7 550 90.17 91.36 86.91 87.02 Example 8 550 93.13 94.47 91.45 88.95 Example 9 550 92.87 93.61 89.64 90.23 Example 10 550 93.17 92.94 90.74 89.14 Example 11 550 97.26 96.37 92.06 92.64 Example 12 550 96.32 97.17 95.24 92.82 Example 13 550 96.06 97.41 95.11 93.32 Example 14 550 96.69 96.06 94.06 93.95 Example 15 550 96.83 96.49 94.76 92.23 Example 16 550 96.72 97.37 93.44 92.31 Example 17 550 96.51 96.17 94.26 92.56 Example 18 550 96.51 96.89 92.29 93.34 Comparative 550 87.47 86.63 73.95 71.54 Example 1 Comparative 550 87.53 82.78 74.25 74.43 Example 2 Comparative 550 89.02 88.97 75.66 77.19 Example 6 Comparative 550 86.97 87.61 73.21 75.47 Example 7 Comparative 550 86.33 87.28 78.51 78.41 Example 8 Comparative 550 86.36 87.13 74.64 74.66 Example 9 Comparative 550 76.23 74.20 55.79 52.81 Example 11 Comparative 550 83.65 81.61 69.17 69.14 Example 12 Comparative 550 81.37 82.56 68.28 72.37 Example 14 2.2 Indoor Efficacy Test of Herbicides (Glufosinate, Ametryn and Topramezone) Tested Herbicides: the ternary compounded herbicides of glufosinate, topramezone and ametryn in different ratios prepared according to Examples 19-36; and the binary or ternary compounded herbicides of glufosinate, topramezone and ametryn and the like in different combinations and ratios according to Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 7, Comparative Example 9, Comparative Example 10, Comparative Example 12 and Comparative Example 13.
Control Herbicides:
200 g/L glufosinate aqueous solutions (commercially available under the trade name of Besulton);
30% topramezone suspension concentrate (commercially available under the trade name of Baowei);
80% ametryn wettable powder (commercially available under the trade name of J
inte).
Table 7 Indoor Control Efficacy (%) of Different Compounded Compositions of Glufosinate, Ametryn and Topramezone against Aegilops tauschii and Fagopyrum tataricum, 30 Days after Application Effective Dosage 30 C (daytime) to 25 C (night) 15 C
(daytime) to 10 C (night) Herbicide Name Fagopyrum Fagopyrum Aegllops tauschii Aegllops tauschii g(a.i.)/ha tataricum at 10 tataricum at 10 with 10-15 tillers with 10-15 tillers leaf-stage leaf-stage Glufosinate 600 80.09 82.35 61.24 63.92 Ametryn 1200 64.18 63.53 47.53 46.30 Topramezone 60 23.72 39.63 17.89 33.22 Example 19 550 98.82 98.75 95.70 95.96 Example 20 550 91.98 92.69 89.96 88.21 Example 21 550 93.15 93.04 89.75 87.08 Example 22 550 93.77 91.84 89.44 87.79 Example 23 550 92.86 93.15 88.32 89.60 Example 24 550 92.46 92.80 89.93 88.32 Example 25 550 92.70 93.14 89.44 89.84 Example 26 550 94.65 95.92 92.51 91.60 Example 27 550 96.45 96.85 92.00 92.15 Example 28 550 95.01 96.86 92.39 91.40 Example 29 550 98.68 99.77 94.31 95.03 Example 30 550 98.97 99.46 96.86 95.30 Example 31 550 97.09 98.82 96.55 94.62 Example 32 550 98.39 99.68 95.18 95.48 Example 33 550 99.20 98.81 96.04 94.99 Example 34 550 98.69 99.08 94.95 95.49 Example 35 550 98.05 98.92 95.46 94.33 Example 36 550 98.23 99.08 94.28 95.42 Comparative 550 87.16 87.34 74.43 75.86 Example 3 Comparative 550 87.71 87.94 75.27 71.20 Example 4 Comparative 550 92.73 93.67 83.32 81.91 Example 5 Comparative 550 86.95 87.38 72.78 74.66 Example 7 Comparative 550 85.65 86.72 73.82 74.35 Example 9 Comparative 550 78.38 79.22 67.03 66.13 Example 10 Comparative 550 82.67 81.47 68.25 68.67 Example 12 Comparative 550 83.29 81.82 69.51 70.65 Example 13 According to the data in Tables 6-7, it can be seen that the herbicidal compositions provided by the Examples of the present application can effectively control malignant weeds even under low temperature conditions. As compared with the same dosage of the single formulations of each active ingredient and the same dosage of the binary compounds of the active ingredients, the present herbicidal compositions show significantly better resistance to low temperature. Moreover, the efficacies achieved by the ternary compounded herbicides are comparable to those achieved by quaternary compounded herbicides. Under the conditions of 30 C (in daytime) to 25 C (at night), as compared with single formulations of each herbicidally active ingredient and compounds in the Comparative Examples, the herbicidal compositions according to the present application have significantly better control efficacy.
3. Effect of Humidity on Efficacy Main Targets: Panicum repens with 30-40 cm height, Persicaria orientalis with 50 cm height Test Method: The test is carried out in different plots of wasteland in Wuwei County, Anhui Province, China, from J une to July. The test field was loamy, flat and medium dry. The soil has a relative humidity of about 40% before application. No other herbicides are applied before and after the test. The weeds are stable in species and evenly distributed. The test field is sheltered from rain, and divided into two halves.
One half is artificially irrigated until the soil is completely wet, and the other half is remained dry (with a relative humidity of about 40%). Tests are designed for two different soil humidities, respectively. If irrigation occurs, water droplets naturally drop and evaporate to dry. The herbicide is applied when no water droplets are maintained on the leaves of the weeds. Each treatment is repeated for 4 times. A region of 30 m2 is sampled from each small block. Upon application, the herbicidal composition is diluted with water to a spray volume of 40 L per mu, and a blank control is formulated by replacing the herbicidal composition with water. Randomized block permutation is applied. The equipment for spraying is a Model AGROLEX HD400 electric sprayer available from AGROLEX PTE Ltd. (Singapore). The spraying is conducted by the stem-leaf spray method. Other conditions for treating each region are completely identical except that the herbicides are applied according to the above scheme.
Evaluation Method: The control efficacy of each treatment against weeds is regularly observed. After 30 days, the fresh weight of the aboveground parts of weeds after each treatment is weighed, and the control efficacy on the target weed is calculated according to the following equation.
control efficacy on basis of fresh weight (%) fresh weight of weeds treated with blank control ¨ fresh weight of weeds treated with herbicide =
______________________________________________________________________________ fresh weight of weeds treated with blank control x 3.1 Field Efficacy Test of Herbicides (Glufosinate, Atrazine and Topramezone) Tested Herbicides: the ternary compounded herbicides of glufosinate, topramezone and atrazine in different ratios prepared according to Examples 1-18; and the binary or ternary compounded herbicides of glufosinate, topramezone and atrazine and the like in different combinations and ratios according to Comparative Example 1, Comparative Example 2, Comparative Example 6, Comparative Examples 7-9, Comparative Example 11, Comparative Example 12 and Comparative Example 14.
Control Herbicides:
200 g/L glufosinate aqueous solutions(commercially available under the trade name of Besulton);
30% topramezone suspension concentrate (commercially available under the trade name of Baowei);
38% atrazine suspension concentrate (commercially available under the trade name of Guochang).
Table 8 Field Control Efficacy (%) of Different Compounded Compositions of Glufosinate, Atrazine and Topramezone, 30 Days after Application Wet Dry Effective Herbicide Name Dosage Persicana Pan icum rep ens Persicana Pan/cum repens with g(a.i.)/ha orientalis with 30- with 30-40 cm orientalis with 30-30-40 cm height 50 cm height height 50 cm height Glufosinate 600 82.05 79.62 63.74 63.16 Atrazine 1600 74.58 75.35 52.21 56.81 Topramezone 60 62.59 59.05 50.41 48.31 Example 1 550 96.26 97.28 92.41 93.13 Example 2 550 90.17 91.23 86.49 86.07 Example 3 550 90.54 90.57 86.77 85.9 Example 4 550 90.29 90.58 85.04 83.81 Example 5 550 90.12 91.06 87.98 86.61 Example 6 550 89.85 89.22 86.21 87.43 Example 7 550 91.1 90.93 86.21 86.11 Example 8 550 94.09 95.47 90.37 90.48 Example 9 550 94.94 93.89 89.52 90.19 Example 10 550 94.23 94.17 90.07 89.07 Example 11 550 96.49 96.04 91.97 90.62 Example 12 550 97.77 96.07 93.74 93.97 Example 13 550 96.96 95.67 90.69 92.73 Example 14 550 96.25 96.68 92.04 93.45 Example 15 550 96.02 97.06 92.64 93.19 Example 16 550 97.37 98.21 92.96 92.29 Example 17 550 96.76 95.98 92.78 93.68 Example 18 550 97.08 96.26 92.74 93.26 Comparative 550 83.18 86.46 72.74 73.19 Example 1 Comparative 550 84.96 86.42 73.47 71.99 Example 2 Comparative 550 86.57 85.44 76.08 77.21 Example 6 Comparative 550 80.71 81.61 66.78 67.41 Example 7 Comparative 550 86.78 85.74 74.09 71.14 Example 8 Comparative 550 85.32 84.71 63.65 65.38 Example 9 Comparative 550 73.89 75.74 55.18 62.05 Example 11 Comparative 550 78.01 79.30 65.01 63.98 Example 12 Comparative 550 75.6 78.42 60.98 63.94 Example 14 3.2 Field Efficacy Test of Herbicides (Glufosinate, Ametryn and Topramezone) Tested Herbicides: the ternary compounded herbicides of glufosinate, topramezone and ametryn in different ratios prepared according to Examples 19-36; and the binary or ternary compounded herbicides of glufosinate, topramezone and ametryn and the like in different combinations and ratios according to Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 7, Comparative Example 9, Comparative Example 10, Comparative Example 12 and Comparative Example 13.
Control Herbicides:
200 g/L aqueous glufosinate aqueous solutions (commercially available under the trade name of Besulton);
30% topramezone suspension concentrate (commercially available under the trade name of Baowei);
80% ametryn wettable powder (commercially available under the trade name of J
inte).
Table 9 Field Control Efficacy (%) of Compounded Compositions of Glufosinate, Ametryn and Topramezone against Panicum repens and Persicaria orientalis, 30 Days after Application Wet Dry Effective Herbicide Name Dosage Pan icum rep ens Persicana Pan icum rep ens Persicana g(a.i.)/ha with 30-40 cm orientalis with 30- with 30-40 cm orientalis with 30-height 50 cm height height 50 cm height Glufosinate 600 82.34 80.16 63.91 63.69 Ametryn 1600 77.23 75.16 54.24 56.45 Topramezone 60 63.57 59.75 50.41 48.83 Example 19 550 99.29 98.37 95.73 94.90 Example 20 550 92.77 93.79 89.32 89.28 Example 21 550 92.41 91.69 88.39 89.24 Example 22 550 92.97 93.94 88.83 89.74 Example 23 550 93.14 93.32 89.85 89.37 Example 24 550 92.68 93.39 89.57 89.50 Example 25 550 93.83 93.10 88.70 89.04 Example 26 550 95.44 97.04 93.85 90.02 Example 27 550 96.65 96.73 91.17 92.06 Example 28 550 96.16 95.83 91.67 91.15 Example 29 550 99.52 98.36 95.23 94.33 Example 30 550 99.65 99.39 95.57 96.54 Example 31 550 98.79 98.48 94.64 95.61 Example 32 550 99.21 98.54 95.98 95.30 Example 33 550 98.34 99.82 94.43 95.57 Example 34 550 99.61 99.51 95.80 96.34 Example 35 550 98.07 99.78 94.35 95.19 Example 36 550 99.07 98.11 94.56 95.41 Comparative 550 86.70 82.89 75.23 74.22 Example 3 Comparative 550 87.59 85.38 75.83 74.11 Example 4 Comparative 550 92.91 91.2 75.48 76.62 Example 5 Comparative 550 79.96 81.55 65.96 66.68 Example 7 Comparative 550 84.75 83.88 63.01 65.17 Example 9 Comparative 550 72.42 74.64 56.67 63.63 Example 10 Comparative 550 77.69 78.87 64.97 63.32 Example 12 Comparative 550 75.80 79.17 61.87 61.91 Example 13 According to the above test data, it can be seen that the herbicidal compositions provided by the present application have excellent control efficacy on various weeds, and can effectively control malignant weeds under both wet and dry conditions.
In order to further demonstrate and illustrate the weed control efficacy of the herbicidal compositions according to the present application, Examples Gl- G29 and Comparative Examples G1-G15 are provided as follows.
Test Example 3 Demonstration of Efficacy Test 1. Efficacy Test in Non-Cultivated Field Tested Herbicides: the herbicidal compositions of Examples G1-G29; and the binary or ternary compounded herbicides of glufosinate, topramezone, atrazine, ametryn, prometryn and the like in different combinations and ratios of Comparative Examples G1-G15 formulated according to Tables 1-2.
Control Herbicides:
200 g/L glufosinate aqueous solutions (commercially available under the trade name of Besulton);
30% topramezone suspension concentrate (commercially available under the trade name of Baowei);
80% ametryn wettable powder (commercially available under the trade name of j inte).
Main Target: Puccinellia distans at 5-leaf stage, Miscanthus sacchariflorus at 8-leaf stage, Euphorbia heterophylla at 6-leaf stage, Heteropappus hispidus at 5-leaf stage, Puccinellia distans at tillering stage, Miscanthus sacchariflorus at jointing stage, Euphorbia heterophylla at 12-leaf stage, and Heteropappus hispidus at bolting stage.
Test Method: The test is carried out in Yuhuang Town (Mianyang, Sichuan Province, China) in late j uly. The application is conducted on a cloudy day with a wind speed of 0.5 m/sec, a minimum temperature of 26.3 C, a maximum temperature of 36.8 C, and an air relative humidity of about 83%. There is no rain for 24 hours after application.
The temperature is 25 C-38 C and the soil relative humidity is 255% during the observation after application. Two test plots with uniform distribution of targets are selected, one plot for elder weeds and the other plot for small-aged weeds.
The two test plots are yellow loam and flat with uniform fertility. No other herbicides are applied before and after the test. The weeds are stable in species and evenly distributed. In the test, the herbicidal compositions of Examples G1-G29 are applied in an amount of 550 g (a.i.)/ha, and the herbicidal compositions of Comparative Examples G1-G15 are applied in an amount of 550 g (a.i.)/ha. Each treatment is repeated for 4 times. A region of 30 m2 is sampled from each small block. Upon application, the herbicidal composition is diluted with water to a spray volume of 40 L per mu, and a blank control is formulated by replacing the herbicidal composition with water. Randomized block permutation is applied. The equipment for spraying is a Model AGROLEX HD400 electric sprayer available from AGROLEX PTE Ltd. (Singapore). The spraying is conducted by the stem-leaf spray method. Other conditions for treating each region are completely identical except that the herbicides are applied according to the above scheme.
Evaluation Method: 35 days after spraying the herbicides, the weeds in the treated regions are identified. 4 points are randomly sampled (the area of the sampling square is 1 m2) from each small block during the investigation. The fresh weight of the aboveground parts of the target weeds within the sampling square are weighed, and the control efficacy on basis of fresh weight is calculated according to the following equation. The results are shown in Table 10.
control efficacy on basis of fresh weight (%) fresh weight of weeds treated with blank control ¨ fresh weight of weeds treated with herbicide =
______________________________________________________________________________ fresh weight of weeds treated with blank control x Table 10 Field Control Efficacy (%) of Herbicidal Compositions of Examples G1-G29 and Comparative Examples G1-G15, 35 Days after Application Small-aged Weeds Elder Weeds Effective Herbicide Miscanthus Miscanthus Euphorbia Dosage Puccinellia Euphorbia Heteropappus Puccinellia Heteropappus Name sacchanflorus sacchanfiorus heterophylla g(a.i.)/ha distans at 5-leaf heterophylla at hispidus at 5-distans at hispidus at 5-at 8-leaf at jointing at 12-leaf stage 6-leaf stage leaf stage tillering stage leaf stage stage stage stage Glufosinate C 600 70.98 73.43 75.09 72.64 65.11 66.34 65.24 65.47 Topramezone 56.12 47.78 55.33 19.03 27.93 60 53.67 27.80 28.16 B
Ametryn A 1200 60.87 63.32 67.98 62.53 45.00 46.23 45.13 45.36 Example G1 550 96.23 97.68 97.34 98.89 94.36 93.59 93.49 94.72 Example G2 550 91.89 94.34 94.00 93.55 89.02 91.25 89.15 91.38 Example G3 550 92.45 94.90 93.56 94.11 91.58 89.81 90.71 89.94 Example G4 550 93.67 95.12 94.78 95.33 90.80 91.03 90.93 91.16 Example G5 550 93.89 95.34 94.00 94.55 90.02 89.25 90.15 90.38 Example G6 550 93.45 94.90 93.56 93.11 91.58 90.81 89.71 89.94 Example G7 550 93.98 94.43 93.09 93.64 88.11 91.34 90.24 90.47 Example G8 550 94.87 93.32 93.98 93.53 89.00 90.23 89.13 89.36 Example G9 550 93.44 93.89 92.55 92.10 87.57 89.80 91.70 90.93 Example G10 550 94.45 93.90 94.56 93.11 93.58 89.81 92.71 91.94 Example G11 550 94.23 93.00 92.66 92.61 91.15 90.71 89.51 90.21 Example G12 550 95.98 96.75 95.41 95.36 90.90 90.46 91.26 91.96 Example G13 550 97.32 98.09 99.75 96.70 94.24 93.80 93.60 92.37 Example G14 550 98.09 97.86 98.52 97.47 94.01 93.57 93.37 94.07 Example G15 550 95.87 94.64 95.30 94.25 90.79 91.35 92.15 92.85 Example G16 550 94.98 96.75 95.41 96.36 92.90 91.46 93.26 92.96 Example G17 550 95.44 94.21 95.87 95.82 91.36 91.92 92.72 92.42 Example G18 550 96.87 98.64 97.30 97.25 92.79 93.35 93.15 94.85 Example G19 550 95.78 97.55 99.21 96.16 92.70 91.26 94.06 94.76 Example G20 550 96.98 99.75 98.41 98.36 91.90 93.46 92.26 93.96 Example G21 550 97.79 99.81 98.47 99.35 93.12 95.01 93.91 94.71 Example G22 550 98.34 99.36 98.02 99.90 93.67 95.56 94.46 95.26 Example G23 550 96.45 96.47 98.13 97.01 92.78 91.67 93.57 93.37 Example G24 550 98.98 99.00 97.66 99.54 94.31 96.20 95.10 95.9 Example G25 550 99.31 99.33 97.99 99.87 94.64 96.53 95.43 96.23 Example G26 550 97.17 99.19 98.85 99.73 92.50 94.39 93.29 94.09 Example G27 550 98.69 99.71 98.37 99.25 94.02 95.91 94.81 95.61 Example G28 550 98.76 96.78 98.44 97.32 92.09 93.98 93.88 92.68 Example G29 550 97.32 96.34 98.00 96.88 93.65 92.54 93.44 93.24 Comparative 89.01 90.67 91.01 71.66 67.46 550 90.78 70.11 68.76 Example G1 Comparative 91.21 90.87 91.21 71.86 70.66 550 92.98 69.31 70.96 Example G2 Comparative 84.68 84.34 82.68 71.33 72.13 550 83.45 70.78 73.43 Example G3 Comparative 91.68 90.34 89.68 69.33 76.13 550 92.45 71.78 74.43 Example G4 Comparative 89.68 88.34 90.68 72.33 71.13 550 90.45 70.78 71.43 Example G5 Comparative 90.13 89.79 90.13 73.78 72.58 550 91.90 72.23 72.88 Example G6 Comparative 92.90 90.56 93.90 88.55 90.35 550 93.67 87.00 88.65 Example G7 Comparative 91.89 89.55 91.89 75.54 78.34 550 90.66 78.99 76.64 Example G8 Comparative 93.10 92.76 93.10 86.75 85.55 550 94.87 85.20 85.85 Example G9 Comparative 87.68 88.34 82.68 79.33 75.13 550 86.45 78.78 75.43 Example G10 Comparative 89.46 89.12 87.66 77.45 76.35 550 88.01 79.67 73.95 Example G11 Comparative 84.34 84.00 83.54 78.33 75.23 550 87.89 79.55 74.83 Example G12 Comparative 86.35 88.01 86.55 71.34 70.24 550 84.90 69.56 70.84 Example G13 Comparative 82.67 85.33 81.87 46.66 50.56 550 87.22 45.88 53.16 Example G14 Comparative 83.78 84.44 83.98 68.77 73.67 550 88.33 72.99 74.27 Example G15 As shown in Table 10, the test results show that the herbicidal compositions provided by the Examples of the present application based on the specific ratios of glufosinate, ametryn, and topramezone have significantly better control efficacy 35 days after application against weeds such as Puccinellia distans at 5-leaf stage, Miscanthus sacchariflorus at 8-leaf stage, Euphorbia heterophylla at 6-leaf stage, Heteropappus hispidus at 5-leaf stage, and the like, as compared with the single formulation of individual ingredients and binary and other multi-ingredient compounds.

The herbicidal compositions provided by the Examples of the present application show significantly better control efficacy 35 days after application against Puccinellia distans at ti I leri ng stage, Miscanthus sacchariflorus at jointing stage, Euphorbia heterophylla at 12-leaf stage and Heteropappus hispidus at bolting stage as compared with the single formulations of individual ingredients and binary and other multi-ingredient compounds. In addition, the herbicidal compositions provided by the Examples of the present application show a comparable level of control efficacy as the quaternary compound of Comparative Example G7.
The test results in Table 10 further demonstrate that the herbicidal compositions according to the present application with glufosinate, ametryn and topramezone at a mass ratio of 15-40:2-16:0.05-3.2 have significantly synergistic effect. In addition, not only the herbicidal spectrum is broadened, but also the control efficacy against elder weeds in actual use is improved.
2. Demonstration of Efficacy Test in Low Temperature Environment Tested Herbicides: the herbicidal compositions of Examples G1-G29; and the binary or ternary compounded herbicides of glufosinate, topramezone, atrazine, ametryn, prometryn and the like in different combinations and ratios of Comparative Examples G1- G15 are formulated according to Tables 1-2.
Control Herbicides:
200 g/L glufosinate aqueous solution(commercially available under the trade name of Besulton);
30% topramezone suspension concentrate (commercially available under the trade name of Baowei);
80% ametryn wettable powder (commercially available under the trade name of j inte).
Main Target: Pseudosclerochloa kengiana with 4 tillers and Geranium carolinianum at 10-leaf stage.
Test Method: As used is the method for treating stems and leaves of an indoor potted plant. Filling a specified amount of soil into a plastic pot, wherein the plastic pot has a height of 10 cm and a diameter of 15 cm, and spot sowing a specified amount of seeds of Pseudosclerochloa kengiana and Geranium carolinianum in the pots respectively, 25 seeds in each pot; cultivating in an artificial climate chamber, removing redundant plants after emergence so as to retain 10 plants in each pot; when the weeds grow to the desired stage for test, treating them by spraying using Model 3WP-2000 mobile spraying tower (Nozzle Model TP6501E, pressure of air compressor: 3 kg/cm2, flow rate: 390 ml/min) (each treatment is repeated for 8 times); after treatment, dividing the treated plants into two groups, wherein one group is placed in an artificial climate chamber at 30 C (daytime) to 25 C (night), and the other group is placed in an artificial climate chamber at 15 C (daytime) to 10 C (night). The illumination intensity is 0 LX
(night) to 7500 LX (daytime). The soil relative humidity for both test groups is 60-70%
during the test.
Evaluation Method: The control efficacy of each treatment against weeds is regularly observed. After 30 days, the fresh weight of the aboveground parts of weeds after each treatment is weighed, and the control efficacy on the target weed is calculated according to the following equation. The results are shown in Table 11.
control efficacy on basis of fresh weight (%) fresh weight of weeds treated with blank control - fresh weight of weeds treated with herbicide = x100 fresh weight of weeds treated with blank control Table 11 Indoor Control Efficacy (%) of Herbicidal Compositions of Examples G1-G29 and Comparative Examples G1-G15, 30 Days after Application Effective 30 C (daytime) to 25 C (night) 15 C (daytime) to 10 C (night) Herbicide Dosage Pseudosclerochloa Geranium Pseudosclerochloa Geranium Name g(a.i.)/ha kengiana carolinianum kengiana carolinianum Glufosinate C 600 78.39 80.51 55.06 62.67 Topramezone 60 18.08 34.20 16.77 29.36 B
Ametryn A 1200 62.28 56.40 50.95 43.56 Example G1 550 98.82 97.94 95.72 96.92 Example G2 550 93.49 93.61 92.39 90.58 Example G3 550 94.05 94.17 92.96 91.14 Example G4 550 93.28 94.40 90.21 92.36 Example G5 550 94.49 94.62 92.41 91.58 Example G6 550 94.06 93.18 91.98 92.14 Example G7 550 93.59 93.71 91.52 92.67 Example G8 550 94.49 94.61 92.43 92.56 Example G9 550 92.05 93.17 93.97 92.13 Example G10 550 93.07 94.19 92.00 91.14 Example G11 550 94.71 93.83 93.67 92.77 Example G12 550 95.46 96.58 94.41 93.52 Example G13 550 98.79 98.91 96.72 95.86 Example G14 550 97.56 98.68 95.50 96.63 Example G15 550 95.34 96.46 94.28 93.41 Example G16 550 95.47 95.59 95.43 94.52 Example G17 550 94.92 95.05 94.88 94.98 Example G18 550 98.34 97.46 95.28 93.41 Example G19 550 98.27 98.39 94.23 95.32 Example G20 550 99.49 99.61 95.47 95.52 Example G21 550 99.56 99.68 95.56 96.57 Example G22 550 98.10 97.22 97.33 96.12 Example G23 550 99.19 99.31 98.15 96.23 Example G24 550 99.74 99.86 94.74 95.76 Example G25 550 98.57 99.19 96.07 95.09 Example G26 550 98.93 99.05 95.46 96.95 Example G27 550 98.46 98.58 95.32 95.47 Example G28 550 99.50 99.62 96.47 94.54 Example G29 550 99.05 99.18 95.02 94.10 Comparative 550 88.55 90.67 79.36 80.67 Example G1 Comparative 550 89.72 87.84 82.57 80.87 Example G2 Comparative 550 86.18 90.30 81.01 79.34 Example G3 Comparative 550 89.19 87.31 82.03 80.34 Example G4 Comparative 550 88.20 90.32 83.05 81.34 Example G5 Comparative 550 89.66 89.78 81.53 82.79 Example G6 Comparative 550 91.44 92.56 83.31 84.56 Example G7 Comparative 550 89.42 89.54 79.28 80.55 Example G8 Comparative 550 92.63 93.75 88.50 89.76 Example G9 Comparative 550 88.23 89.35 73.13 72.34 Example G10 Comparative 87.90 85.02 77.79 74.01 Example G11 Comparative 86.78 85.90 75.67 72.89 Example G12 Comparative 83.76 85.88 69.62 71.90 Example G13 Comparative 550 73.10 74.22 51.98 53.22 Example G14 Comparative 81.22 82.34 67.12 68.33 Example G15 The test results are shown in Table 11. The test results demonstrate that, under the condition of 30 C (daytime) to 25 C (night), as compared with single formulations of individual herbicidal active ingredients and Comparative Examples G1-G15, the control efficacy 30 days after application of the Examples of the present application is significantly better than the single formulations; slightly better than Comparative Example G1-G6 and G8-G15; and comparative to Comparative Example G7.
Under the low temperature condition of 15 C (daytime) to 10 C (night), as compared with single formulations of individual herbicidal active ingredients and Comparative Examples G1-G15, the control efficacy 30 days after application of the Examples Gl-G29 of the present application is significantly better than Comparative Example G1-G15. Specifically, Comparative Examples G1-G6 differ from Examples G1-G29 in the ratios of the herbicidal active ingredients. The efficacies of Comparative Examples G1-G6 are significantly inferior to the efficacies of Examples G1-G29. Although Comparative Example G7 comprises pyroxasulfone in addition to the three ingredients, namely glufosinate, topramezone and amytryn, the efficacy thereof decreases at low temperatures. The herbicidal compositions provided in the present application are significantly more effective when used at low temperatures, thus further demonstrating that the herbicidal compositions of the present application are effective against a wide range of weeds at low temperatures.
3. Effect of Humidity on Efficacy Test Herbicides: the herbicidal compositions of Examples G1-G29; and the binary or ternary compounded herbicides of glufosinate, topramezone, atrazine, ametryn, prometryn and the like in different combinations and ratios of Comparative Examples G1- G15 are formulated according to Table 1-2.
Control Herbicides:
200 g/L glufosinate aqueous solution(commercially available under the trade name of Besulton);
30% topramezone suspension concentrate (commercially available under the trade name of Baowei);
80% ametryn wettable powder (commercially available under the trade name of J
inte).
Main Target: glufosinate-resistant Eleusine indica with 10-15 tillers, Zinnia elegans at 10-12-leaf stage.
Test Method: The test is carried out in a flower and wood Field in Boluo County (Huizhou, Guangdong Province, China) in September. The application is conducted on a sunny day with a wind speed of 0.4 m/sec, a minimum temperature of 22 C, a maximum temperature of 33 C. There is no rain for 24 hours after application.
The temperature is 20 C-35 C and the air relative humidity is 70%-80% during the observation after application. The test field was loamy, flat and medium dry.
The soil relative humidity is about 42%. The weeds in the test plots are stable in species, and evenly distributed. Due to the use of single formulation of glufosinate year by year, Eleusine indica has become resistant to glufosinate. The test field is sheltered from rain before application, and divided into two halves. One half is artificially irrigated until the soil is completely wet, and the other half is remained dry (a soil relative humidity of about 42% as determined by a soil detector). Tests are designed for two different soil humidities, respectively. If irrigation occurs, water droplets naturally drop and are evaporated to dry. The herbicides are applied when no water droplets are maintained on the leaves of the weeds. Each treatment is repeated for 4 times. A region of 30 m2 is sampled from each small block. Upon application, the herbicidal composition is diluted with water to a spray volume of 40 L per mu, and a blank control is formulated by replacing the herbicidal composition with water. Randomized block permutation is applied. The equipment for spraying is a Model AGROLEX HD400 electric sprayer available from AGROLEX PTE Ltd. (Singapore). The spraying is conducted by the stem-leaf spray method. Other conditions for treating each region are completely identical except that the herbicides are applied according to the above scheme. In the test, the wetted region has a soil relative humidity of about 70%-80% 2 days after application, and a soil relative humidity of about 60%-70% 3-7 days after application.
Evaluation Method: The control efficacy of each treatment against weeds is regularly observed. After 30 days, the fresh weight of the aboveground parts of weeds after each treatment is weighed, and the control efficacy of the herbicide on the target weed is calculated according to the following equation.
control efficacy on basis of fresh weight (%) fresh weight of weeds treated with blank control ¨ fresh weight of weeds treated with herbicide =
______________________________________________________________________________ fresh weight of weeds treated with blank control x Table 12 Field Control Efficacy (%) of Herbicidal Compositions of Examples G1-G29 and Comparative Examples G1-G15, 30 Days after Application 1Herbicide Name Effective Dosage Wet Dry g(a.i.)/ha resistant Eleusine Zinnia elegans resistant Eleusine Zinnia elegans id/ca id/ca Glufosinate C 600 50.34 79.63 23.32 61.21 Topramezone B 60 54.54 45.82 45.99 33.88 Ametryn A 1200 73.32 74.60 53.19 55.07 Example G1 550 96.02 97.35 94.74 94.68 Example G2 550 91.58 92.91 91.40 90.34 Example G3 550 92.14 92.47 91.96 90.91 Example G4 550 93.12 92.45 92.19 91.14 Example G5 550 93.32 92.65 92.40 90.35 Example G6 550 94.56 93.89 92.97 90.92 Example G7 550 94.89 92.22 93.50 91.45 Example G8 550 93.12 94.45 91.40 92.36 Example G9 550 93.23 92.56 92.96 90.91 Example G10 550 93.22 93.55 91.98 91.93 Example G11 550 93.12 94.45 92.86 93.82 Example G12 550 95.67 96.00 93.54 93.49 Example G13 550 97.41 98.75 95.28 96.23 Example G14 550 99.52 98.85 96.57 95.53 Example G15 550 95.36 96.32 94.50 93.84 Example G16 550 95.87 95.21 94.14 92.09 Example G17 550 96.43 95.77 94.31 92.26 Example G18 550 97.88 98.22 95.75 96.71 Example G19 550 97.28 97.62 96.14 96.10 Example G20 550 97.23 96.57 95.10 95.07 Example G21 550 98.47 96.82 96.00 95.96 Example G22 550 98.22 96.57 96.12 95.08 Example G23 550 96.31 97.64 95.20 94.16 Example G24 550 99.46 97.81 96.76 95.73 Example G25 550 99.19 98.54 96.09 94.05 Example G26 550 99.05 98.40 96.94 95.91 Example G27 550 98.58 97.92 95.46 94.43 Example G28 550 96.62 97.96 94.52 95.47 Example G29 600 97.18 97.02 95.07 95.51 Comparative Example 550 81.88 82.55 68.36 67.31 Comparative Example 550 80.44 83.12 68.51 69.45 Comparative Example 550 79.20 84.52 66.96 70.89 Comparative Example 550 78.81 80.49 69.97 71.91 Comparative Example 550 79.92 81.6 66.99 69.93 Comparative Example 550 78.69 82.36 63.47 71.41 Comparative Example 550 83.27 83.94 75.25 73.2 Comparative Example 550 81.54 82.22 73.22 71.16 Comparative Example 550 91.53 90.86 88.44 87.38 Comparative Example 550 76.57 80.23 65.06 63.01 Comparative Example 550 74.02 75.35 64.85 65.8 Comparative Example 550 79.3 79.63 63.73 64.67 Comparative Example 550 39.98 71.31 26.69 61.63 Comparative Example 550 68.22 72.55 50.05 61.99 Comparative Example 550 45.34 74.67 28.18 62.12 According to the test results shown in Table 12, under wet conditions, as compared with single formulations of individual herbicidal active ingredients and Comparative Examples G1-G15, the control efficacies 30 days after application of the herbicidal compositions of Examples G1-G29 are significantly better than the single formulations;
significantly better than Comparative Examples G1-G6 and G8-G15; and better than Comparative Example G7. Under dry conditions (a soil relative humidity of about 42%), as compared with single formulations of individual herbicidal active ingredients and Comparative Examples G1-G27, the control efficacies 30 days after application of Examples G1-G29 are significantly better than the single formulations; and better than Comparative Examples G1-G15, thus further demonstrating that the herbicidal compositions provided by the Examples of the present application provide superior weed control under dry conditions, and are particularly effective against glufosinate-resistant weeds.
4. Indoor Activity Determination against Resistant Eleusine indica Tested Herbicides: some herbicidal compositions of Examples G1-G29; and some compounded herbicides of glufosinate, topramezone, atrazine, ametryn, prometryn and the like in different combinations and ratios of Comparative Examples Gl- G15 are formulated according to Tables 1-2.
Control Herbicides:
200 g/L glufosinate aqueous solution (commercially available under the trade name of Besulton);
30% topramezone suspension concentrate (commercially available under the trade name of Baowei);
80% ametryn wettable powder (commercially available under the trade name of j inte).
Plant Materials for Test: glufosinate-resistant Eleusine indica, which are weed seeds collected from the field in Boluo County (Huizhou, Guangdong, glufosinate blank treated small block) by the researchers in Lier Crop Science Co., Ltd.
Sichuan, China, and stored in a storehouse.
Test Method: As used is the method for treating stems and leaves of a potted plant (NY/T 1155.4-2006). Filling a specified amount of soil into a plastic pot, wherein the plastic pot has a height of 16.2 cm and a diameter of 18.5 cm; sowing 25-30 seeds of the target weed into the plastic pot, covering them with 0.5-1 cm thick fine soil, and then cultivating in a greenhouse. After emergence, the seedlings are thinned out, merely retaining 10 plants in each pot. When growing to 10 to 12-leaf stage, the weeds are treated by spraying stems and leaves (each treatment is repeated for 4 times).
The treatment without any herbicide is considered as the control.
Application Method: stem-leaf spray treatment. The spray applies Model 3WPSH-500D spray tower for bioassay (manufactured by Nanjing Agricultural Mechanization Research Institute of the Chinese Ministry of Agriculture) with a disc diameter of 50 cm, a rotation speed of the main shaft of 6 rpm, an aperture diameter of the nozzle of 0.3 mm, a spray pressure of 0.3 MPa, a diameter of the sprayed droplet of 100 pm, and a nozzle flow rate of 90 mL/min. After treatment, the test materials are cultured in an artificial climate chamber at a temperature of 30 C (during the day) to 25 C
(at night) and an air humidity of 70-80%. The growth of the target weeds is observed regularly.
Evaluation Method: The control efficacy of each treatment against weeds is regularly observed. After 30 days, the fresh weight of the aboveground parts of weeds after each treatment is weighed, and the control efficacy of the herbicide on the target weed is calculated according to the following equation. The results are shown in Table control efficacy on basis of fresh weight (%) fresh weight of weeds treated with blank control ¨ fresh weight of weeds treated with herbicide _______________________________________________________________________________ _ 100 fresh weight of weeds treated with blank control x Table 13 Control Efficacy (%) of Some Herbicidal Compositions of Examples G1-G29 and Comparative Examples G1-G15 against Resistant Eleusine indica, 30 Days after Application Effective Dosage Control Efficacy On Basis Of Fresh Herbicide Name g(a.i.)/ha Weight of Resistant Eleusine Mdica Glufosinate C 600 47.75 Topramezone B 60 80.23 Ametryn A 1200 76.44 Example G1 550 99.85 Example G3 550 91.63 Example G5 550 92.47 Example G7 550 93.43 Example G9 550 92.26 Example G11 550 93.14 Example G13 550 100.00 Example G14 550 100.00 Example G15 550 95.24 Example G16 550 94.73 Example G17 550 95.26 Example G18 550 98.78 Example G20 550 99.21 Example G22 550 100.00 Example G24 550 100.00 Example G26 550 100.00 Comparative 550 86.32 Example G1 Comparative 550 85.47 Example G2 Comparative 550 85.36 Example G4 Comparative 550 84.59 Example G5 Comparative 550 85.25 Example G6 Comparative 550 86.89 Example G7 Comparative 550 83.11 Example G10 Comparative 550 83.64 Example G11 Comparative 550 67.37 Example G13 Comparative 550 79.21 Example G14 Comparative 550 71.21 Example G15 According to the test results shown in Table 13, glufosinate is less effective against the tested resistant Eleusine indica. The herbicide compositions provided by the Examples of the present application are significantly effective against resistant Eleusine indica than the single formulations and the Comparative Examples listed in Table 13.
It can be seen that the herbicidal compositions provided by the Examples of the present application have more superior weed control efficacy against resistant Eleusine indica.
According to the above Examples, it can be seen that the herbicidal compositions provided in the present application, which are formulated by combining the three active ingredients in specific ratios, can effectively control the elder weeds and malignant weeds. In particular, under low temperature and dry conditions, they can still maintain significantly excellent weed control efficacy. They can be applied to stubborn weeds in non-cultivated lands or orchards, and are less affected by the limitations of geographical and climatic conditions. Moreover, good weed control efficacy can be achieved by combining merely three herbicidally active ingredients, and ternary compounded herbicides which are in compliance with the "Measures on the Management of Pesticide Registration" can be provided. It is of great significance of popularization and agricultural practical value.
The herbicidal compositions provided in the embodiments of the present application and the use thereof have been described in detail above. The illustrations for the above embodiments are only intended to help understand the technical solutions and core concepts of the present application. Those skilled in the art should understand that the technical solutions described in the foregoing embodiments can be modified, or part of the technical features therein can be equivalently replaced, provided that these modifications or replacements do not make the essence of corresponding technical solutions depart from the scope of the technical solutions of the various embodiments of the present application.

Claims

What is claimed is:

1. A herbicidal composition comprising herbicidally active ingredients, wherein the herbicidally active ingredients comprise active ingredient A, active ingredient B and active ingredient C, wherein active ingredient A is atrazine or ametryn, active ingredient B is topramezone, and active ingredient C is glufosinate or a salt thereof, and in the herbicidal composition, the mass ratio of the herbicidally active ingredients A, B and C is (2-16):(0.05-3.2):(15-40).

2. The herbicidal composition according to claim 1, wherein the herbicidally active ingredients consist of active ingredient A, active ingredient B and active ingredient C, and the mass ratio of the herbicidally active ingredients A, B and C is (2-16):(0.05-3.2):(15-40).

3. The herbicidal composition according to claim 1 or 2, wherein the herbicidal composition, the mass ratio of the herbicidally active ingredients A, B and C
is (3-12):(0.075-2.5):(20-35).

4. The herbicidal composition according to claim 1 or 2, wherein in the herbicidal composition, the mass ratio of the herbicidally active ingredients A, B and C
is (4-9):(0.1-1.5):(25-30).

5. The herbicidal composition according to claim 1 or 2, wherein the mass percentage of the herbicidally active ingredients is 10%-80%, based on the total mass of the herbicidal composition.

6. The herbicidal composition according to claim 5, wherein the mass percentage of the herbicidally active ingredients is 30%-60%, based on the total mass of the herbicidal composition.

7. The herbicidal composition according to claim 1 or 2, wherein the herbicidal composition further comprises formulants for pesticides.

8. Use of the herbicidal composition according to any one of claims 1-7, wherein the herbicidal composition is used to control weeds in non-cultivated lands or orchards.

9. The use according to claim 8, wherein the weeds are annual or perennial weeds.

10. The use according to claim 8, wherein the herbicidal composition is applied in an amount of 300-900 g(a.i.)/ha.

11. The use according to claim 8, wherein the herbicidal composition is applied at a soil humidity of 30%-85%.

12. The use according to claim 8, wherein the herbicidal composition is applied at an ambient temperature of 10 C-30 C.