CA2339595A1 - Systeme binaire d'expression virale dans des plantes - Google Patents
Systeme binaire d'expression virale dans des plantes Download PDFInfo
- Publication number
- CA2339595A1 CA2339595A1 CA002339595A CA2339595A CA2339595A1 CA 2339595 A1 CA2339595 A1 CA 2339595A1 CA 002339595 A CA002339595 A CA 002339595A CA 2339595 A CA2339595 A CA 2339595A CA 2339595 A1 CA2339595 A1 CA 2339595A1
- Authority
- CA
- Canada
- Prior art keywords
- site
- gene
- viral
- replication
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000014509 gene expression Effects 0.000 title claims abstract description 268
- 230000003612 virological effect Effects 0.000 title claims description 96
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 351
- 230000001105 regulatory effect Effects 0.000 claims abstract description 137
- 108700019146 Transgenes Proteins 0.000 claims abstract description 63
- 241000196324 Embryophyta Species 0.000 claims description 298
- 230000010076 replication Effects 0.000 claims description 169
- 108020004414 DNA Proteins 0.000 claims description 111
- 230000006798 recombination Effects 0.000 claims description 99
- 238000005215 recombination Methods 0.000 claims description 99
- 230000030279 gene silencing Effects 0.000 claims description 96
- 230000009261 transgenic effect Effects 0.000 claims description 87
- 241000700605 Viruses Species 0.000 claims description 78
- 239000012634 fragment Substances 0.000 claims description 78
- 101150036876 cre gene Proteins 0.000 claims description 69
- 102000004169 proteins and genes Human genes 0.000 claims description 68
- 230000029812 viral genome replication Effects 0.000 claims description 63
- 108091026890 Coding region Proteins 0.000 claims description 62
- 101710150114 Protein rep Proteins 0.000 claims description 57
- 101710152114 Replication protein Proteins 0.000 claims description 57
- 108010052160 Site-specific recombinase Proteins 0.000 claims description 54
- 241000702463 Geminiviridae Species 0.000 claims description 47
- 241000702295 Tomato golden mosaic virus Species 0.000 claims description 45
- 238000000034 method Methods 0.000 claims description 45
- 238000013518 transcription Methods 0.000 claims description 45
- 230000035897 transcription Effects 0.000 claims description 45
- 241000702449 African cassava mosaic virus Species 0.000 claims description 36
- 241000709992 Potato virus X Species 0.000 claims description 34
- 238000012226 gene silencing method Methods 0.000 claims description 32
- 230000004913 activation Effects 0.000 claims description 31
- 241001493065 dsRNA viruses Species 0.000 claims description 27
- 108010091086 Recombinases Proteins 0.000 claims description 24
- 230000001965 increasing effect Effects 0.000 claims description 24
- 102000018120 Recombinases Human genes 0.000 claims description 23
- 239000000539 dimer Substances 0.000 claims description 22
- 230000001131 transforming effect Effects 0.000 claims description 18
- 230000001419 dependent effect Effects 0.000 claims description 16
- 108700008625 Reporter Genes Proteins 0.000 claims description 14
- 230000001939 inductive effect Effects 0.000 claims description 13
- 238000011161 development Methods 0.000 claims description 10
- 230000010502 episomal replication Effects 0.000 claims description 10
- 230000000903 blocking effect Effects 0.000 claims description 9
- 230000018109 developmental process Effects 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 238000012250 transgenic expression Methods 0.000 claims description 6
- 241000238631 Hexapoda Species 0.000 claims description 5
- 206010034133 Pathogen resistance Diseases 0.000 claims description 5
- 108050006628 Viral movement proteins Proteins 0.000 claims description 5
- 230000002363 herbicidal effect Effects 0.000 claims description 5
- 239000004009 herbicide Substances 0.000 claims description 5
- LWTDZKXXJRRKDG-KXBFYZLASA-N (-)-phaseollin Chemical compound C1OC2=CC(O)=CC=C2[C@H]2[C@@H]1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-KXBFYZLASA-N 0.000 claims description 4
- 108030001386 Helper-component proteinases Proteins 0.000 claims description 4
- 108010016634 Seed Storage Proteins Proteins 0.000 claims description 4
- 241000701489 Cauliflower mosaic virus Species 0.000 claims description 3
- 108010058731 nopaline synthase Proteins 0.000 claims description 3
- 230000008121 plant development Effects 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 108090000565 Capsid Proteins Proteins 0.000 claims description 2
- 101710163504 Phaseolin Proteins 0.000 claims description 2
- 101710172711 Structural protein Proteins 0.000 claims description 2
- LWTDZKXXJRRKDG-UHFFFAOYSA-N phaseollin Natural products C1OC2=CC(O)=CC=C2C2C1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-UHFFFAOYSA-N 0.000 claims description 2
- 230000003321 amplification Effects 0.000 abstract description 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 75
- 108091093088 Amplicon Proteins 0.000 description 66
- 239000013598 vector Substances 0.000 description 62
- 239000013612 plasmid Substances 0.000 description 57
- 210000001519 tissue Anatomy 0.000 description 50
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 33
- 230000001404 mediated effect Effects 0.000 description 28
- 230000009466 transformation Effects 0.000 description 28
- 101710132601 Capsid protein Proteins 0.000 description 27
- 101710094648 Coat protein Proteins 0.000 description 27
- 101710125418 Major capsid protein Proteins 0.000 description 27
- 101710141454 Nucleoprotein Proteins 0.000 description 27
- 101710083689 Probable capsid protein Proteins 0.000 description 27
- 108700026244 Open Reading Frames Proteins 0.000 description 24
- 239000000047 product Substances 0.000 description 23
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 21
- 239000002585 base Substances 0.000 description 20
- 230000014616 translation Effects 0.000 description 20
- 241000208125 Nicotiana Species 0.000 description 17
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 17
- 238000004519 manufacturing process Methods 0.000 description 17
- 230000000694 effects Effects 0.000 description 16
- 241000589158 Agrobacterium Species 0.000 description 14
- 108091028043 Nucleic acid sequence Proteins 0.000 description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 14
- 210000000349 chromosome Anatomy 0.000 description 14
- 150000007523 nucleic acids Chemical class 0.000 description 14
- 238000013519 translation Methods 0.000 description 14
- 239000002299 complementary DNA Substances 0.000 description 13
- 108020004999 messenger RNA Proteins 0.000 description 13
- 230000003362 replicative effect Effects 0.000 description 12
- 230000002103 transcriptional effect Effects 0.000 description 12
- 241000207746 Nicotiana benthamiana Species 0.000 description 11
- 208000015181 infectious disease Diseases 0.000 description 11
- 239000002245 particle Substances 0.000 description 11
- 238000011144 upstream manufacturing Methods 0.000 description 11
- 101800000653 Helper component proteinase Proteins 0.000 description 10
- 108700009124 Transcription Initiation Site Proteins 0.000 description 10
- 240000008042 Zea mays Species 0.000 description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 10
- 235000005822 corn Nutrition 0.000 description 10
- 239000002773 nucleotide Substances 0.000 description 10
- 125000003729 nucleotide group Chemical group 0.000 description 10
- 239000013603 viral vector Substances 0.000 description 10
- 108700026226 TATA Box Proteins 0.000 description 9
- 238000010367 cloning Methods 0.000 description 9
- 230000002068 genetic effect Effects 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 102000053602 DNA Human genes 0.000 description 8
- 108060001084 Luciferase Proteins 0.000 description 8
- 108020005091 Replication Origin Proteins 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 238000003780 insertion Methods 0.000 description 8
- 230000037431 insertion Effects 0.000 description 8
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 8
- 235000010469 Glycine max Nutrition 0.000 description 7
- 239000005089 Luciferase Substances 0.000 description 7
- 108091081024 Start codon Proteins 0.000 description 7
- 108700025695 Suppressor Genes Proteins 0.000 description 7
- 108700005077 Viral Genes Proteins 0.000 description 7
- 150000001413 amino acids Chemical group 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 230000001627 detrimental effect Effects 0.000 description 7
- 230000000977 initiatory effect Effects 0.000 description 7
- 230000010354 integration Effects 0.000 description 7
- 230000033001 locomotion Effects 0.000 description 7
- 244000068988 Glycine max Species 0.000 description 6
- 108091023045 Untranslated Region Proteins 0.000 description 6
- 230000002759 chromosomal effect Effects 0.000 description 6
- 230000035772 mutation Effects 0.000 description 6
- 230000004544 DNA amplification Effects 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 108020004682 Single-Stranded DNA Proteins 0.000 description 5
- 241000723792 Tobacco etch virus Species 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- 239000013256 coordination polymer Substances 0.000 description 5
- 239000003623 enhancer Substances 0.000 description 5
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 5
- 239000010931 gold Substances 0.000 description 5
- 229910052737 gold Inorganic materials 0.000 description 5
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 230000009885 systemic effect Effects 0.000 description 5
- 238000000844 transformation Methods 0.000 description 5
- 241000724256 Brome mosaic virus Species 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- 108010051219 Cre recombinase Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 241000219146 Gossypium Species 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- 244000061176 Nicotiana tabacum Species 0.000 description 4
- 101900259239 Potato virus X Coat protein Proteins 0.000 description 4
- 108010076504 Protein Sorting Signals Proteins 0.000 description 4
- 240000003768 Solanum lycopersicum Species 0.000 description 4
- 108091036066 Three prime untranslated region Proteins 0.000 description 4
- 108020000999 Viral RNA Proteins 0.000 description 4
- 208000036142 Viral infection Diseases 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000000692 anti-sense effect Effects 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 230000006690 co-activation Effects 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 230000006801 homologous recombination Effects 0.000 description 4
- 238000002744 homologous recombination Methods 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 229930027917 kanamycin Natural products 0.000 description 4
- 229960000318 kanamycin Drugs 0.000 description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 4
- 229930182823 kanamycin A Natural products 0.000 description 4
- 230000021121 meiosis Effects 0.000 description 4
- 230000008520 organization Effects 0.000 description 4
- 230000002085 persistent effect Effects 0.000 description 4
- 108010001545 phytoene dehydrogenase Proteins 0.000 description 4
- 230000008488 polyadenylation Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000013605 shuttle vector Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000001629 suppression Effects 0.000 description 4
- 230000009385 viral infection Effects 0.000 description 4
- 210000000605 viral structure Anatomy 0.000 description 4
- 230000028604 virus induced gene silencing Effects 0.000 description 4
- KQRMGBSYWRUESQ-UHFFFAOYSA-N (2-chlorophenyl)sulfonylurea Chemical compound NC(=O)NS(=O)(=O)C1=CC=CC=C1Cl KQRMGBSYWRUESQ-UHFFFAOYSA-N 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 3
- 108020005345 3' Untranslated Regions Proteins 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 3
- 101150062179 II gene Proteins 0.000 description 3
- 108091092195 Intron Proteins 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 108020005120 Plant DNA Proteins 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- 108020005038 Terminator Codon Proteins 0.000 description 3
- 241000030538 Thecla Species 0.000 description 3
- 108010067390 Viral Proteins Proteins 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000001072 colon Anatomy 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 230000037433 frameshift Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000017074 necrotic cell death Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 231100000208 phytotoxic Toxicity 0.000 description 3
- 230000000885 phytotoxic effect Effects 0.000 description 3
- 210000000745 plant chromosome Anatomy 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000008707 rearrangement Effects 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 230000010474 transient expression Effects 0.000 description 3
- 230000014621 translational initiation Effects 0.000 description 3
- SNBCLPGEMZEWLU-QXFUBDJGSA-N 2-chloro-n-[[(2r,3s,5r)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methyl]acetamide Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CNC(=O)CCl)[C@@H](O)C1 SNBCLPGEMZEWLU-QXFUBDJGSA-N 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 241000724328 Alfalfa mosaic virus Species 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- 108700003860 Bacterial Genes Proteins 0.000 description 2
- 241000723655 Cowpea mosaic virus Species 0.000 description 2
- LMKYZBGVKHTLTN-NKWVEPMBSA-N D-nopaline Chemical compound NC(=N)NCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O LMKYZBGVKHTLTN-NKWVEPMBSA-N 0.000 description 2
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 2
- 241000450599 DNA viruses Species 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 108091029795 Intergenic region Proteins 0.000 description 2
- 241000726306 Irus Species 0.000 description 2
- 241000702489 Maize streak virus Species 0.000 description 2
- 108700001094 Plant Genes Proteins 0.000 description 2
- 108010059820 Polygalacturonase Proteins 0.000 description 2
- 241000710078 Potyvirus Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 241000710155 Turnip yellow mosaic virus Species 0.000 description 2
- 108020005202 Viral DNA Proteins 0.000 description 2
- 241000702661 Wound tumor virus Species 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 230000007541 cellular toxicity Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 101150087654 chrnd gene Proteins 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000005860 defense response to virus Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000006353 environmental stress Effects 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 231100000221 frame shift mutation induction Toxicity 0.000 description 2
- 230000004345 fruit ripening Effects 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- -1 light Substances 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 210000002706 plastid Anatomy 0.000 description 2
- 230000001124 posttranscriptional effect Effects 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000005070 ripening Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000009758 senescence Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 210000000352 storage cell Anatomy 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000007919 viral pathogenicity Effects 0.000 description 2
- GEWDNTWNSAZUDX-WQMVXFAESA-N (-)-methyl jasmonate Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-WQMVXFAESA-N 0.000 description 1
- NUMXHEUHHRTBQT-AATRIKPKSA-N 2,4-dimethoxy-1-[(e)-2-nitroethenyl]benzene Chemical compound COC1=CC=C(\C=C\[N+]([O-])=O)C(OC)=C1 NUMXHEUHHRTBQT-AATRIKPKSA-N 0.000 description 1
- 101150110188 30 gene Proteins 0.000 description 1
- 244000105975 Antidesma platyphyllum Species 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 101100107610 Arabidopsis thaliana ABCF4 gene Proteins 0.000 description 1
- 241000020089 Atacta Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000702325 Beet curly top virus Species 0.000 description 1
- 241000702451 Begomovirus Species 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 101000713368 Bovine immunodeficiency virus (strain R29) Protein Tat Proteins 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 101800000592 Capsid protein 3 Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 108090000994 Catalytic RNA Proteins 0.000 description 1
- 102000053642 Catalytic RNA Human genes 0.000 description 1
- 241000736839 Chara Species 0.000 description 1
- 241000272161 Charadriiformes Species 0.000 description 1
- 241000251204 Chimaeridae Species 0.000 description 1
- 241001414720 Cicadellidae Species 0.000 description 1
- 108091062157 Cis-regulatory element Proteins 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 101710190853 Cruciferin Proteins 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 241000702461 Curtovirus Species 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
- 102000016607 Diphtheria Toxin Human genes 0.000 description 1
- 108010053187 Diphtheria Toxin Proteins 0.000 description 1
- 101150071673 E6 gene Proteins 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 108010087894 Fatty acid desaturases Proteins 0.000 description 1
- 102000009114 Fatty acid desaturases Human genes 0.000 description 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
- 101150066002 GFP gene Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 235000009438 Gossypium Nutrition 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- VWFWJPJMAFRNET-MQWMPURMSA-N IACI Chemical compound C1=CC=C2C([C@@H](O)[C@@H]3CC4CCN3C[C@@H]4CC)=CC=NC2=C1NC(=O)C1=CC([125I])=C(N=[N+]=[N-])C=C1O VWFWJPJMAFRNET-MQWMPURMSA-N 0.000 description 1
- 108010054278 Lac Repressors Proteins 0.000 description 1
- 241000272168 Laridae Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
- 241000702459 Mastrevirus Species 0.000 description 1
- 241000940612 Medina Species 0.000 description 1
- 101710140999 Metallocarboxypeptidase inhibitor Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 101000713102 Mus musculus C-C motif chemokine 1 Proteins 0.000 description 1
- 101710202365 Napin Proteins 0.000 description 1
- 101100293593 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) nar-1 gene Proteins 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 108010047956 Nucleosomes Proteins 0.000 description 1
- 101710089395 Oleosin Proteins 0.000 description 1
- 101710091688 Patatin Proteins 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 108010081996 Photosystem I Protein Complex Proteins 0.000 description 1
- 108010060806 Photosystem II Protein Complex Proteins 0.000 description 1
- 108020005089 Plant RNA Proteins 0.000 description 1
- 101100271190 Plasmodium falciparum (isolate 3D7) ATAT gene Proteins 0.000 description 1
- 241000053208 Porcellio laevis Species 0.000 description 1
- 241000710007 Potexvirus Species 0.000 description 1
- 241000549435 Pria Species 0.000 description 1
- 101710127370 Probable head completion protein 1 Proteins 0.000 description 1
- 102100022985 Protein arginine N-methyltransferase 1 Human genes 0.000 description 1
- 108020004412 RNA 3' Polyadenylation Signals Proteins 0.000 description 1
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
- 241000702632 Rice dwarf virus Species 0.000 description 1
- 101100068078 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GCN4 gene Proteins 0.000 description 1
- 102100035254 Sodium- and chloride-dependent GABA transporter 3 Human genes 0.000 description 1
- 101710104417 Sodium- and chloride-dependent GABA transporter 3 Proteins 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 108091027544 Subgenomic mRNA Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 241000723848 Tobamovirus Species 0.000 description 1
- 101000623262 Trypanosoma brucei brucei Uncharacterized 22 kDa protein in aldolase locus Proteins 0.000 description 1
- 101710196023 Vicilin Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 229920000180 alkyd Polymers 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- 150000008331 benzenesulfonamides Chemical class 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- GPRBEKHLDVQUJE-VINNURBNSA-N cefotaxime Chemical compound N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C(O)=O)=O)C(=O)/C(=N/OC)C1=CSC(N)=N1 GPRBEKHLDVQUJE-VINNURBNSA-N 0.000 description 1
- 230000000453 cell autonomous effect Effects 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000001055 chewing effect Effects 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229940088530 claforan Drugs 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229930191339 dianthin Natural products 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000005712 elicitor Substances 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 239000002895 emetic Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012458 free base Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000010437 gem Substances 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 101150054900 gus gene Proteins 0.000 description 1
- 235000009424 haa Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 238000013101 initial test Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000014634 leaf senescence Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- GEWDNTWNSAZUDX-UHFFFAOYSA-N methyl 7-epi-jasmonate Natural products CCC=CCC1C(CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-UHFFFAOYSA-N 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 230000000952 necrogenic effect Effects 0.000 description 1
- 210000001623 nucleosome Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000000063 preceeding effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000019525 primary metabolic process Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 101150075980 psbA gene Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000012882 rooting medium Substances 0.000 description 1
- 230000024053 secondary metabolic process Effects 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 102000023888 sequence-specific DNA binding proteins Human genes 0.000 description 1
- 108091008420 sequence-specific DNA binding proteins Proteins 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8217—Gene switch
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8237—Externally regulated expression systems
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8237—Externally regulated expression systems
- C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/12011—Geminiviridae
- C12N2750/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'invention concerne des systèmes d'expression de transgènes dans des plantes. Ces systèmes sont composés de deux éléments, individuellement héréditaires, intégrés dans les chromosomes. Ces systèmes sont utiles pour obtenir l'expression régulée de gènes étrangers par amplification génique dans le tissu de la plante.
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10155898P | 1998-09-23 | 1998-09-23 | |
| US60/101,558 | 1998-09-23 | ||
| US13008699P | 1999-04-20 | 1999-04-20 | |
| US69/130,086 | 1999-04-20 | ||
| US15025599P | 1999-08-23 | 1999-08-23 | |
| US60/150,255 | 1999-08-23 | ||
| PCT/US1999/021989 WO2000017365A2 (fr) | 1998-09-23 | 1999-09-22 | Systeme binaire d'expression virale dans des plantes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2339595A1 true CA2339595A1 (fr) | 2000-03-30 |
Family
ID=27379204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002339595A Abandoned CA2339595A1 (fr) | 1998-09-23 | 1999-09-22 | Systeme binaire d'expression virale dans des plantes |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1115870A2 (fr) |
| CA (1) | CA2339595A1 (fr) |
| HU (1) | HUP0104697A3 (fr) |
| NZ (1) | NZ510594A (fr) |
| WO (1) | WO2000017365A2 (fr) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6632980B1 (en) * | 1997-10-24 | 2003-10-14 | E. I. Du Pont De Nemours And Company | Binary viral expression system in plants |
| AU2001243939B2 (en) * | 2000-03-28 | 2006-06-15 | Queensland University Of Technology | A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences |
| DE60131075T2 (de) | 2000-03-28 | 2008-07-31 | Queensland University Of Technology, Brisbane | Durch freisetzung in geschlossener, zirkulärer form aus einer grösseren nukleotidsequenz charakterisiertes konstrukt, welches die ortsspezifische und/oder entwicklungsspezifische, regulierte expression selektierter, genetischer sequenzen erlaubt |
| US7468475B2 (en) | 2000-06-16 | 2008-12-23 | Schmuelling Thomas | Method for modifying plant morphology, biochemistry and physiology |
| DE10049587A1 (de) | 2000-10-06 | 2002-05-02 | Icon Genetics Ag | Vektorsystem für Pflanzen |
| US7560622B2 (en) | 2000-10-06 | 2009-07-14 | Pioneer Hi-Bred International, Inc. | Methods and compositions relating to the generation of partially transgenic organisms |
| DE10061150A1 (de) | 2000-12-08 | 2002-06-13 | Icon Genetics Ag | Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen |
| DE10102389A1 (de) | 2001-01-19 | 2002-08-01 | Icon Genetics Ag | Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen |
| DE10109354A1 (de) * | 2001-02-27 | 2002-09-05 | Icon Genetics Ag | Rekombinante virale Schaltersysteme |
| DE10114209A1 (de) | 2001-03-23 | 2002-12-05 | Icon Genetics Ag | Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren |
| DE10115507A1 (de) | 2001-03-29 | 2002-10-10 | Icon Genetics Ag | Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus |
| DE10121283B4 (de) * | 2001-04-30 | 2011-08-11 | Icon Genetics GmbH, 80333 | Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen |
| DE10132780A1 (de) | 2001-07-06 | 2003-01-16 | Icon Genetics Ag | Plastidäre Genexpression über autonom replizierende Vektoren |
| DE10143238A1 (de) | 2001-09-04 | 2003-03-20 | Icon Genetics Ag | Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente |
| DE10143237A1 (de) | 2001-09-04 | 2003-03-20 | Icon Genetics Ag | Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente) |
| US7238854B2 (en) | 2002-04-11 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Method of controlling site-specific recombination |
| AU2003247696A1 (en) | 2002-07-01 | 2004-01-19 | E.I. Du Pont De Nemours And Company | Method of controlling gene silencing using site-specific recombination |
| PL2121935T3 (pl) | 2006-09-25 | 2011-12-30 | Schmuelling Thomas | Transkrypcyjne represory szlaków sygnałowych cytokinin i ich zastosowanie |
| EP2272862B1 (fr) | 2009-07-10 | 2012-03-21 | Freie Universität Berlin | Rock2 et rock3, deux nouvelles variantes de gain de fonction des récepteurs de cytokine AHK2 et AHK3 |
| EP2418283A1 (fr) | 2010-08-07 | 2012-02-15 | Nomad Bioscience GmbH | Procédé de transfection de plantes |
| EP2584042A1 (fr) | 2011-10-17 | 2013-04-24 | Nomad Bioscience GmbH | Production, stockage et utilisation d'enzymes dégradant la paroi cellulaire |
| EP2647715A1 (fr) | 2012-04-03 | 2013-10-09 | Nomad Bioscience GmbH | Agrobacterium pour transfection transitoire de plantes entières |
| EP3097783B1 (fr) | 2015-05-26 | 2019-11-13 | Nomad Bioscience GmbH | Colicine m pour le contrôle de ehec |
| EP3372091A1 (fr) | 2017-03-07 | 2018-09-12 | Nomad Bioscience GmbH | Procédé de réduction de la contamination d'un objet par clostridium |
| EP3378485A1 (fr) | 2017-03-24 | 2018-09-26 | Nomad Bioscience GmbH | Bactériocines pour le contrôle de salmonella enterica |
| EP3752185A1 (fr) | 2018-02-15 | 2020-12-23 | Icon Genetics GmbH | Composition immunogène et vaccin pour générer une réponse immunitaire à un norovirus |
| ES2953232T3 (es) | 2019-03-12 | 2023-11-08 | Icon Genetics Gmbh | Partículas similares a norovirus con estabilidad mejorada |
| BR112021024197A2 (pt) | 2019-06-06 | 2022-04-26 | Nomad Bioscience Gmbh | Klebicinas para o controle de klebsiella |
| CA3189366A1 (fr) | 2020-07-16 | 2022-01-20 | Nomad Bioscience Gmbh | Produits pour consommation orale a teneur reduite en sucre |
| EP4248987A1 (fr) | 2022-03-21 | 2023-09-27 | Nomad Bioscience GmbH | Bactériocines chimériques et procédé de lutte contre les pseudomonas |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3686633T2 (de) * | 1985-10-25 | 1993-04-15 | David Matthew Bisaro | Pflanzenvektoren. |
| NL9001711A (nl) * | 1989-10-03 | 1991-05-01 | Clovis Matton N V | Genetische manipulaties met recombinant dna, dat van rna virus afgeleide sequenties omvat. |
| CA2156720A1 (fr) * | 1993-02-26 | 1994-09-01 | Jean C. Kridl | Systeme d'expression de gene a base de geminivirus |
| GB9405768D0 (en) * | 1994-03-23 | 1994-05-11 | Univ Leicester | Viral replicon |
| GB9626858D0 (en) * | 1996-12-24 | 1997-02-12 | Gatsby Plant Science Innovatio | Transcriptional regulation in plants |
| GB9703146D0 (en) * | 1997-02-14 | 1997-04-02 | Innes John Centre Innov Ltd | Methods and means for gene silencing in transgenic plants |
| NZ504510A (en) * | 1997-11-18 | 2002-10-25 | Pioneer Hi Bred Int | Methods and compositions for increasing efficiency of excision of a viral replicon from T-DNA that is transferred to a plant by agroinfection |
-
1999
- 1999-09-22 NZ NZ510594A patent/NZ510594A/xx unknown
- 1999-09-22 HU HU0104697A patent/HUP0104697A3/hu unknown
- 1999-09-22 WO PCT/US1999/021989 patent/WO2000017365A2/fr not_active Ceased
- 1999-09-22 CA CA002339595A patent/CA2339595A1/fr not_active Abandoned
- 1999-09-22 EP EP99969445A patent/EP1115870A2/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| HUP0104697A2 (hu) | 2002-03-28 |
| NZ510594A (en) | 2002-09-27 |
| WO2000017365A2 (fr) | 2000-03-30 |
| WO2000017365A8 (fr) | 2000-10-12 |
| EP1115870A2 (fr) | 2001-07-18 |
| HUP0104697A3 (en) | 2003-12-29 |
| WO2000017365A3 (fr) | 2000-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6632980B1 (en) | Binary viral expression system in plants | |
| CA2339595A1 (fr) | Systeme binaire d'expression virale dans des plantes | |
| US6077992A (en) | Binary viral expression system in plants | |
| ES2267175T3 (es) | Procedcimiento y medios para bloqueo genetico en las plantas transgenicas. | |
| AU731330B2 (en) | Binary viral expression system in plants | |
| JP2000515748A (ja) | 植物細胞における導入遺伝子の発現可変性を減少させる方法 | |
| JP2004536573A (ja) | 植物における遺伝子発現を調節するための組換えウイルススイッチ | |
| KR100477413B1 (ko) | 진핵세포로 전달된 외래 dna의 개선된 통합방법 | |
| WO2000056895A1 (fr) | Promoteur de la virose (cotton leaf curl virus clcuv) d'un cotonnier | |
| US7238854B2 (en) | Method of controlling site-specific recombination | |
| US7267979B2 (en) | Method of controlling gene silencing using site specific recombination | |
| AU2010211450B2 (en) | Plant transformation using DNA minicircles | |
| JP4797141B2 (ja) | 選択された遺伝子配列の部位特異的発現および／または発生上調節される発現を可能にする、より大きいヌクレオチド配列から閉環状で放出することができる構築物 | |
| WO2011031370A1 (fr) | Amélioration de la reproduction de la tolérance à la chaleur chez des plantes | |
| CN101113452A (zh) | 植物花特异性启动子及其应用 | |
| MXPA01002374A (en) | Binary viral expression system in plants | |
| AU1441000A (en) | Binary viral expression system in plants | |
| Yuan et al. | Crop plants transformation methods | |
| Lee | New Binary Ti Vectors with Co-directional Replicons for Agrobacterium Tumefaciens-mediated Transformation of Higher Plants | |
| MXPA00003537A (en) | Binary viral expression system in plants | |
| Hegedus | 10 pORE Modular Vectors for Plant Transformation | |
| MXPA99007512A (en) | Methods and means for gene silencing in transgenic plants | |
| AU2001243939A1 (en) | A construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |