[go: up one dir, main page]

CA2282735A1 - Amas d'acarbose acb issu d'actinoplanes espece se 50/110 - Google Patents

Amas d'acarbose acb issu d'actinoplanes espece se 50/110 Download PDF

Info

Publication number
CA2282735A1
CA2282735A1 CA002282735A CA2282735A CA2282735A1 CA 2282735 A1 CA2282735 A1 CA 2282735A1 CA 002282735 A CA002282735 A CA 002282735A CA 2282735 A CA2282735 A CA 2282735A CA 2282735 A1 CA2282735 A1 CA 2282735A1
Authority
CA
Canada
Prior art keywords
fragment
dna
plasmid
acarbose
pass
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002282735A
Other languages
English (en)
Inventor
Anneliese Crueger
Heiner Apeler
Werner Schroder
Hermann Pape
Klaus Goeke
Wolfgang Piepersberg
Jurgen Distler
Paz Marta Diaz-Guardamino Uribe
Martin Jarling
Ansgar Stratmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2282735A1 publication Critical patent/CA2282735A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/365—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinoplanes (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des gènes de biosynthèse issus du groupe de gènes acarbose provenant d'actinoplanes espèce SE 50/110, leur isolement à partir d'actinoplanes d'espèce ou de producteurs de pseudo-oligosaccharides. L'invention concerne un procédé d'isolement de ces gènes de biosynthèse, les protéines codées par lesdits gènes, l'expression des protéines dans des souches-hôtes hétérologues et l'utilisation des gènes de biosynthèse d'acarbose pour optimiser le procédé.
CA002282735A 1997-02-28 1998-02-16 Amas d'acarbose acb issu d'actinoplanes espece se 50/110 Abandoned CA2282735A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19708127.4 1997-02-28
DE19708127A DE19708127A1 (de) 1997-02-28 1997-02-28 Acarbose acb-Cluster: Isolierung von weiteren Genen der Acarbose Biosynthese und des Acarbose-Stoffwechsels aus Actinoplanes sp. SE 50/110 sowie deren Verwendung
PCT/EP1998/000862 WO1998038313A1 (fr) 1997-02-28 1998-02-16 Amas d'acarbose acb issu d'actinoplanes espece se 50/110

Publications (1)

Publication Number Publication Date
CA2282735A1 true CA2282735A1 (fr) 1998-09-03

Family

ID=7821816

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002282735A Abandoned CA2282735A1 (fr) 1997-02-28 1998-02-16 Amas d'acarbose acb issu d'actinoplanes espece se 50/110

Country Status (15)

Country Link
EP (1) EP0968294A1 (fr)
KR (1) KR20000075777A (fr)
CN (1) CN1249001A (fr)
AU (1) AU6296898A (fr)
BG (1) BG103672A (fr)
BR (1) BR9807640A (fr)
CA (1) CA2282735A1 (fr)
CZ (1) CZ9903054A3 (fr)
DE (1) DE19708127A1 (fr)
HU (1) HUP0000889A2 (fr)
IL (1) IL131433A0 (fr)
NO (1) NO994164L (fr)
PL (1) PL335369A1 (fr)
WO (1) WO1998038313A1 (fr)
ZA (1) ZA981659B (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130302855A1 (en) * 2010-08-04 2013-11-14 Bayer Intellectual Property Gmbh Genomics of actinoplanes utahensis
CN106167814A (zh) * 2016-08-31 2016-11-30 河北华荣制药有限公司 一种提高阿卡波糖发酵单位的方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0796915A3 (fr) * 1996-03-22 1999-04-14 Bayer Ag Procédé de préparation et utilisation d'acarviosyl-transferase dans la conversion d'homologues d'acarbose et dans la préparation d'homologues d'acarbose
CN106566796B (zh) * 2016-10-28 2020-11-10 上海交通大学 阿卡波糖生产菌Actinoplanes spp.的遗传操作体系
CN114555811B (zh) * 2019-10-16 2025-03-25 拜耳公司 用于改善阿卡波糖形成的方法
CN112592878B (zh) * 2020-12-25 2022-08-26 上海交通大学 增强正调控蛋白基因表达以提高阿卡波糖发酵水平的方法
CN113444670A (zh) * 2021-07-28 2021-09-28 山东鲁抗医药股份有限公司 一种高活性阿卡波糖产生菌的筛选方法和培养方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19507214A1 (de) * 1995-03-02 1996-10-31 Bayer Ag Acarbose-Biosynthesegene aus Actinoplanes sp., Verfahren zu ihrer Isolierung sowie ihre Verwendung
DE19622783A1 (de) * 1996-06-07 1997-12-11 Hoechst Ag Isolierung der Biosynthesegene für Pseudo-Oligosaccharide aus Streptomyces glaucescens GLA.O und ihre Verwendung

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130302855A1 (en) * 2010-08-04 2013-11-14 Bayer Intellectual Property Gmbh Genomics of actinoplanes utahensis
US9719064B2 (en) 2010-08-04 2017-08-01 Bayer Intellectual Property Gmbh Genomics of actinoplanes utahensis
CN106167814A (zh) * 2016-08-31 2016-11-30 河北华荣制药有限公司 一种提高阿卡波糖发酵单位的方法
CN106167814B (zh) * 2016-08-31 2019-08-09 河北华荣制药有限公司 一种提高阿卡波糖发酵单位的方法

Also Published As

Publication number Publication date
HUP0000889A2 (en) 2000-07-28
NO994164D0 (no) 1999-08-27
KR20000075777A (ko) 2000-12-26
EP0968294A1 (fr) 2000-01-05
DE19708127A1 (de) 1998-09-03
AU6296898A (en) 1998-09-18
BG103672A (en) 2000-05-31
NO994164L (no) 1999-08-27
IL131433A0 (en) 2001-01-28
ZA981659B (en) 1998-09-10
WO1998038313A1 (fr) 1998-09-03
CN1249001A (zh) 2000-03-29
PL335369A1 (en) 2000-04-25
CZ9903054A3 (cs) 1999-11-17
BR9807640A (pt) 2000-03-21

Similar Documents

Publication Publication Date Title
van Wezel et al. Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3 (2) is controlled by malR, a member of the lacI–galR family of regulatory genes
CN110305881B (zh) 一种聚酮类化合物neoenterocins的生物合成基因簇及其应用
Li et al. polR, a pathway-specific transcriptional regulatory gene, positively controls polyoxin biosynthesis in Streptomyces cacaoi subsp. asoensis
Suzuki et al. Regulation of lankamycin biosynthesis in Streptomyces rochei by two SARP genes, srrY and srrZ
CN111117942B (zh) 一种产林可霉素的基因工程菌及其构建方法和应用
ES2276427T3 (es) Aislamiento de los genes de biosintesis de seudo-oligosacaridos de streptomyces glaucescens gla.o y su uso.
CA2282735A1 (fr) Amas d'acarbose acb issu d'actinoplanes espece se 50/110
Barrasa et al. The aminonucleoside antibiotic A201A is inactivated by a phosphotransferase activity from Streptomyces capreolus NRRL 3817, the producing organism: isolation and molecular characterization of the relevant encoding gene and its DNA flanking regions
TW201406951A (zh) 用於製造重組１１－去－Ｏ－甲基托馬黴素（１１－ｄｅ－Ｏ－ｍｅｔｈｙｌｔｏｍａｙｍｙｃｉｎ）之方法
LAUER et al. A putative enolpyruvyl transferase gene involved in nikkomycin biosynthesis
EP4467651A1 (fr) Procédé de régulation et de contrôle d'un composé flavonoïde synthétique hétérologue et son utilisation
AU706116B2 (en) Acarbose biosynthesis genes from Actinoplanes sp., process for the isolation thereof and the use thereof
HK1203970A1 (en) New actinomycete integrative and conjugative element from actinoplanes sp.se50/110 as plasmid for genetic transformation of related actinobacteria
MXPA97002141A (es) Procedimiento para la produccion asi como para eluso de acarviosil-transferasa en la transformacion de homologos de acarbosa en acarbosa, para la produccion de homologos de acarbosa
Tan et al. Increasing demeclocycline production in Streptomyces aureofaciens by manipulating the expression of a novel SARP family regulator and its genes
EP1443113A1 (fr) Procede d'obtention d'indolocarbazoles au moyen de genes biosynthetiques de rebecamicine
KR20000070723A (ko) 클라불란산 생산이 증가된 미생물
CA2200421A1 (fr) Methode pour preparer l'acarviosyl-transferase et l'utiliser dans la conversion des homologues de l'acarbose en acarbose et la preparation d'homologues de l'acarbose
CN115247179B (zh) 一种聚酮化合物骨架及其后修饰物的生物合成基因簇及其应用
Li et al. Identification and application of a strong bidirectional acmN2p promoter from actinomycin D-producing streptomycetes
US7608448B2 (en) Method and cloning vectors utilizing intergeneric conjugation for manipulation of actinomycetes biosynthesis genes
MXPA99007728A (en) Acarbose (acb) cluster from actinoplanes
EP2247740B1 (fr) Gènes codant pour la voie biosynthétique pour la production d'etnangien
CN116656582A (zh) 产泰乐菌素类似物的工程菌及其构建方法与应用
見﨑裕也 Functional analysis of transcriptiopnal regulators for secondary metabolites production in Actinomycetes

Legal Events

Date Code Title Description
FZDE Discontinued