CA2161337A1 - Tampon de lyse directe et detection de la viremie dans le plasma pour vih-1 - Google Patents
Tampon de lyse directe et detection de la viremie dans le plasma pour vih-1Info
- Publication number
- CA2161337A1 CA2161337A1 CA002161337A CA2161337A CA2161337A1 CA 2161337 A1 CA2161337 A1 CA 2161337A1 CA 002161337 A CA002161337 A CA 002161337A CA 2161337 A CA2161337 A CA 2161337A CA 2161337 A1 CA2161337 A1 CA 2161337A1
- Authority
- CA
- Canada
- Prior art keywords
- hiv
- plasma
- pcr
- rna
- assay
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 239000003643 water by type Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5862393A | 1993-05-06 | 1993-05-06 | |
| US08/058,623 | 1993-05-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2161337A1 true CA2161337A1 (fr) | 1994-11-24 |
Family
ID=22017952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002161337A Abandoned CA2161337A1 (fr) | 1993-05-06 | 1994-04-28 | Tampon de lyse directe et detection de la viremie dans le plasma pour vih-1 |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0698082A4 (fr) |
| JP (1) | JPH08510004A (fr) |
| AU (1) | AU6776994A (fr) |
| CA (1) | CA2161337A1 (fr) |
| WO (1) | WO1994026867A1 (fr) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2184270C (fr) * | 1994-03-10 | 1999-08-17 | Daniel Louis Kacian | Methode pour supprimer l'inhibition des reactions via les enzymes, grace a des detergents ioniques |
| FR2763957B1 (fr) * | 1997-05-29 | 2001-09-14 | Bio Merieux | Procede pour isoler un materiel intracellulaire et procede de traitement d'un materiel nucleique |
| US5958677A (en) * | 1997-07-28 | 1999-09-28 | The New York Blood Center, Inc. | Method for purifying viral nucleic acids |
| DE19752961C1 (de) * | 1997-11-28 | 1999-07-15 | Fraunhofer Ges Forschung | Verfahren und Vorrichtung zum Aufschluß biologischer Zellen zur Extraktion und Analyse der Zellinhalte |
| AU4312201A (en) * | 1999-12-10 | 2001-06-18 | Genespan Corporation | Isolation and purification of nucleic acids |
| EP2163652B2 (fr) | 2002-01-28 | 2023-05-31 | Life Technologies Corporation | Production des extraits biologiques bruts utilisant protease, convenant pour la synthèse de cDNA |
| US20050277121A1 (en) | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
| DE102005015005A1 (de) | 2005-04-01 | 2006-10-05 | Qiagen Gmbh | Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe |
| US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
| US8211637B2 (en) | 2008-12-19 | 2012-07-03 | Life Technologies Corporation | Proteinase K inhibitors, methods and compositions therefor |
| CN102575220B (zh) | 2009-09-03 | 2015-09-16 | 贝克顿·迪金森公司 | 用于直接化学裂解的方法和组合物 |
| CN109402240B (zh) * | 2019-01-08 | 2020-08-25 | 圣湘生物科技股份有限公司 | 核酸释放剂、核酸pcr扩增方法和pcr扩增试剂盒 |
| WO2021221109A1 (fr) * | 2020-04-30 | 2021-11-04 | タカラバイオ株式会社 | Procédé de détection de virus à arn |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2791367B2 (ja) * | 1988-04-21 | 1998-08-27 | マイクロプローブ・コーポレーション | 核酸抽出方法 |
| US5182377A (en) * | 1988-09-09 | 1993-01-26 | Hoffmann-La Roche Inc. | Probes for detection of human papillomavirus |
| US5231015A (en) * | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
| EP0488243A1 (fr) * | 1990-11-30 | 1992-06-03 | Sanwa Kagaku Kenkyusho Co., Ltd. | Procédé d'extraction du génome du virus d'un échantillon derivé d'un corps vivant infecté par le virus et procédé de détection du génome |
| US5213796A (en) * | 1991-05-06 | 1993-05-25 | Dana Farber Cancer Institute | Assay for polyomavirus in humans and uses thereof |
| US5175094A (en) * | 1991-08-01 | 1992-12-29 | Becton Dickinson And Company | Increased expression of HBcAg |
-
1994
- 1994-04-28 CA CA002161337A patent/CA2161337A1/fr not_active Abandoned
- 1994-04-28 JP JP6525476A patent/JPH08510004A/ja active Pending
- 1994-04-28 WO PCT/US1994/004676 patent/WO1994026867A1/fr not_active Ceased
- 1994-04-28 EP EP94915927A patent/EP0698082A4/fr not_active Withdrawn
- 1994-04-28 AU AU67769/94A patent/AU6776994A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP0698082A1 (fr) | 1996-02-28 |
| EP0698082A4 (fr) | 1997-12-17 |
| WO1994026867A1 (fr) | 1994-11-24 |
| AU6776994A (en) | 1994-12-12 |
| JPH08510004A (ja) | 1996-10-22 |
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