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BR112016029178A2 - composições e métodos para a expressão de rnas guia de crispr usando o promotor h1 - Google Patents

composições e métodos para a expressão de rnas guia de crispr usando o promotor h1

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Publication number
BR112016029178A2
BR112016029178A2 BR112016029178A BR112016029178A BR112016029178A2 BR 112016029178 A2 BR112016029178 A2 BR 112016029178A2 BR 112016029178 A BR112016029178 A BR 112016029178A BR 112016029178 A BR112016029178 A BR 112016029178A BR 112016029178 A2 BR112016029178 A2 BR 112016029178A2
Authority
BR
Brazil
Prior art keywords
promoter
compositions
methods
expression
crispr
Prior art date
Application number
BR112016029178A
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English (en)
Inventor
Zack Donald
Ranganathan Vinod
Original Assignee
Univ Johns Hopkins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Johns Hopkins filed Critical Univ Johns Hopkins
Publication of BR112016029178A2 publication Critical patent/BR112016029178A2/pt

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    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
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Abstract

a matéria presentemente refere-se a composições e métodos para a expressão de rnas guia de crispr usando-se o promotor h1. em particular, são providos composições e métodos para o uso do promotor h1 para expressar rna guia de crispr (grna) com especificidade alterada no nucleotídeo 5', bem como o uso da sequência de promotor h1 como um promotor bidirecional para expressar a nuclease de cas9 e o grna simultaneamente. são também providos composições e métodos para a expressão e a regulação de expressão de grna in vivo através do uso de ribozimas de rna e de aptazimas reguladas.
BR112016029178A 2014-06-16 2015-06-16 composições e métodos para a expressão de rnas guia de crispr usando o promotor h1 BR112016029178A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462012802P 2014-06-16 2014-06-16
PCT/US2015/035964 WO2015195621A1 (en) 2014-06-16 2015-06-16 Compositions and methods for the expression of crispr guide rnas using the h1 promoter

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BR112016029178A2 true BR112016029178A2 (pt) 2017-10-17

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US (5) US9907863B2 (pt)
EP (2) EP3674408A1 (pt)
JP (2) JP6930834B2 (pt)
KR (1) KR102649341B1 (pt)
CN (2) CN106852157B (pt)
AU (2) AU2015277369B2 (pt)
BR (1) BR112016029178A2 (pt)
CA (1) CA2952697A1 (pt)
CL (1) CL2016003247A1 (pt)
DK (1) DK3155101T3 (pt)
EA (1) EA039693B1 (pt)
ES (1) ES2788426T3 (pt)
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