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AU2001229535A1 - Dna polymerase mutant having one or more mutations in the active site - Google Patents

Dna polymerase mutant having one or more mutations in the active site

Info

Publication number
AU2001229535A1
AU2001229535A1 AU2001229535A AU2953501A AU2001229535A1 AU 2001229535 A1 AU2001229535 A1 AU 2001229535A1 AU 2001229535 A AU2001229535 A AU 2001229535A AU 2953501 A AU2953501 A AU 2953501A AU 2001229535 A1 AU2001229535 A1 AU 2001229535A1
Authority
AU
Australia
Prior art keywords
mutations
dna polymerase
active site
polymerase mutant
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001229535A
Other languages
English (en)
Inventor
Lawrence A Loeb
Premal H. Patel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Original Assignee
University of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington filed Critical University of Washington
Publication of AU2001229535A1 publication Critical patent/AU2001229535A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2001229535A 2000-01-14 2001-01-11 Dna polymerase mutant having one or more mutations in the active site Abandoned AU2001229535A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/484,114 US6329178B1 (en) 2000-01-14 2000-01-14 DNA polymerase mutant having one or more mutations in the active site
US09484114 2000-01-14
PCT/US2001/001473 WO2001051621A2 (fr) 2000-01-14 2001-01-11 Adn polymerase mutant comprenant une ou plusieurs mutations dans le site actif

Publications (1)

Publication Number Publication Date
AU2001229535A1 true AU2001229535A1 (en) 2001-07-24

Family

ID=23922797

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001229535A Abandoned AU2001229535A1 (en) 2000-01-14 2001-01-11 Dna polymerase mutant having one or more mutations in the active site

Country Status (5)

Country Link
US (2) US6329178B1 (fr)
EP (1) EP1246924A2 (fr)
JP (1) JP2003519488A (fr)
AU (1) AU2001229535A1 (fr)
WO (1) WO2001051621A2 (fr)

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Also Published As

Publication number Publication date
JP2003519488A (ja) 2003-06-24
US6602695B2 (en) 2003-08-05
EP1246924A2 (fr) 2002-10-09
US6329178B1 (en) 2001-12-11
US20020132249A1 (en) 2002-09-19
WO2001051621A3 (fr) 2002-01-24
WO2001051621A2 (fr) 2001-07-19

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