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AU2001229535A1 - Dna polymerase mutant having one or more mutations in the active site - Google Patents

Dna polymerase mutant having one or more mutations in the active site

Info

Publication number
AU2001229535A1
AU2001229535A1 AU2001229535A AU2953501A AU2001229535A1 AU 2001229535 A1 AU2001229535 A1 AU 2001229535A1 AU 2001229535 A AU2001229535 A AU 2001229535A AU 2953501 A AU2953501 A AU 2953501A AU 2001229535 A1 AU2001229535 A1 AU 2001229535A1
Authority
AU
Australia
Prior art keywords
mutations
dna polymerase
active site
polymerase mutant
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001229535A
Inventor
Lawrence A Loeb
Premal H. Patel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Original Assignee
University of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington filed Critical University of Washington
Publication of AU2001229535A1 publication Critical patent/AU2001229535A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2001229535A 2000-01-14 2001-01-11 Dna polymerase mutant having one or more mutations in the active site Abandoned AU2001229535A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/484,114 US6329178B1 (en) 2000-01-14 2000-01-14 DNA polymerase mutant having one or more mutations in the active site
US09484114 2000-01-14
PCT/US2001/001473 WO2001051621A2 (en) 2000-01-14 2001-01-11 Dna polymerase mutant having one or more mutations in the active site

Publications (1)

Publication Number Publication Date
AU2001229535A1 true AU2001229535A1 (en) 2001-07-24

Family

ID=23922797

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001229535A Abandoned AU2001229535A1 (en) 2000-01-14 2001-01-11 Dna polymerase mutant having one or more mutations in the active site

Country Status (5)

Country Link
US (2) US6329178B1 (en)
EP (1) EP1246924A2 (en)
JP (1) JP2003519488A (en)
AU (1) AU2001229535A1 (en)
WO (1) WO2001051621A2 (en)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US20020102577A1 (en) * 2000-07-31 2002-08-01 Raillard Sun Ai Nucleotide incorporating enzymes
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US6958213B2 (en) * 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7262012B2 (en) 2002-05-17 2007-08-28 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
JP2006504438A (en) * 2002-11-01 2006-02-09 エヴォジェニクス・ピーティーワイ・リミテッド Mutagenesis method using ribavirin and / or RNA replicase
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
DE102004005885A1 (en) * 2004-02-05 2005-10-13 Rheinische Friedrich-Wilhelms-Universität Bonn Mutated DNA polymerases with increased mismatch discrimination
GB0420500D0 (en) * 2004-09-15 2004-10-20 Univ Newcastle DNA polymerases
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
EP1907584A2 (en) * 2005-06-28 2008-04-09 Agencourt Personal Genomics Corporation Methods of producing and sequencing modified polynucleotides
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
DK2171088T3 (en) * 2007-06-19 2016-01-25 Stratos Genomics Inc Nucleic acid sequencing in a high yield by expansion
US8026091B2 (en) 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods
JP5596554B2 (en) * 2007-11-28 2014-09-24 エフ．ホフマン−ラ ロシュ アーゲー Mutant DNA polymerase with improved pyrophosphate degradation activated polymerization (PAP) capability
EP3101124B1 (en) 2008-11-03 2019-06-19 Kapa Biosystems, Inc. Modified type a dna polymerases
WO2010088557A1 (en) 2009-01-29 2010-08-05 Stratos Genomics Inc. High throughput nucleic acid sequencing by expansion and related methods
EP2582802B1 (en) 2010-06-18 2016-01-13 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
CA2802302C (en) 2010-06-18 2016-04-26 F. Hoffman-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
WO2011157438A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increasesed 3'-mismatch discrimination
EP2582808B1 (en) 2010-06-18 2015-04-22 Roche Diagniostics GmbH Dna polymerases with increased 3'-mismatch discrimination
EP2582804B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
JP5926247B2 (en) 2010-06-18 2016-05-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ DNA polymerase with increased 3 'terminal mismatch discrimination
JP5876477B2 (en) 2010-06-18 2016-03-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ DNA polymerase with increased 3 'terminal mismatch discrimination
EP2582803B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
EP2675897B1 (en) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
ES2561885T3 (en) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag Enhanced activity DNA polymerases
US9090883B2 (en) 2011-07-28 2015-07-28 Roche Molecular Systems, Inc. DNA polymerases with improved activity
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
LT2742151T (en) 2011-08-10 2018-02-12 Life Technologies Corporation POLYMEROSE COMPOSITION
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
CN103987844B (en) 2011-12-08 2016-01-20 霍夫曼-拉罗奇有限公司 Have and improve active archaeal dna polymerase
WO2013083262A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
WO2013083264A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
EP3789502A1 (en) 2012-05-02 2021-03-10 Ibis Biosciences, Inc. Dna sequencing
ES2683707T3 (en) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. DNA sequencing
WO2013166304A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Dna sequencing
US9212392B2 (en) 2012-09-25 2015-12-15 Exact Sciences Corporation Normalization of polymerase activity
WO2014152937A1 (en) 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
US10428379B2 (en) 2013-03-15 2019-10-01 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US20150051088A1 (en) 2013-08-19 2015-02-19 Abbott Molecular Inc. Next-generation sequencing libraries
US9932623B2 (en) 2013-08-19 2018-04-03 Abbott Molecular Inc. Nucleotide analogs
CA2930595C (en) 2013-11-14 2023-09-19 Integrated Dna Technologies, Inc. Dna polymerase mutants having enhanced template discrimination activity
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
CN107532124B (en) 2015-03-27 2022-08-09 精密科学公司 Detection of esophageal disorders
CN108350485A (en) 2015-10-30 2018-07-31 精密科学发展有限责任公司 The multiplex amplification detection assay of plasma dna and separation and detection
WO2017192221A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by analysis of methylated dna
JP2019528323A (en) 2016-08-31 2019-10-10 バイオアトラ、エルエルシー Conditionally active polypeptide and method for producing the same
WO2018140329A1 (en) 2017-01-24 2018-08-02 Tsavachidou Dimitra Methods for constructing copies of nucleic acid molecules
CN110475875B (en) 2017-01-27 2024-06-25 精密科学公司 Detection of colon neoplasia by analysis of methylated DNA
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
CN111556900A (en) 2017-12-22 2020-08-18 赛默飞世尔科技波罗的海封闭股份公司 Polymerase chain reaction compositions including amines
WO2019180137A1 (en) 2018-03-21 2019-09-26 F. Hoffmann-La Roche Ag Dna polymerases for efficient and effective incorporation of methylated-dntps
CN112639089B (en) 2018-09-03 2024-02-09 深圳华大生命科学研究院 Recombinant KOD polymerase
CN112703248B (en) 2018-09-13 2025-03-25 豪夫迈·罗氏有限公司 Mutant DNA polymerase with improved strand displacement ability
EP3874033A4 (en) 2018-10-29 2022-08-03 Codexis, Inc. Engineered dna polymerase variants
WO2020092830A1 (en) 2018-10-31 2020-05-07 Illumina, Inc. Polymerases, compositions, and methods of use
JP7527208B2 (en) 2018-12-05 2024-08-02 イラミーナ インコーポレーテッド Polymerases, compositions, and methods of use
BR112021013020A2 (en) 2018-12-31 2021-09-14 Abbott Molecular Inc. IMPROVED AMPLIFICATION OF NUCLEIC ACIDS
CN114651070A (en) 2019-09-16 2022-06-21 精密科学公司 Structure and temperature dependent flap endonuclease substrates
WO2021252603A1 (en) * 2020-06-10 2021-12-16 Rhodx, Inc. Methods for identifying modified bases in a polynucleotide
WO2022194244A1 (en) * 2021-03-19 2022-09-22 Mgi Tech Co., Ltd. Polymerases for efficient incorporation of nucleotides with 3'-phosphate and other 3'-terminators
US20220403450A1 (en) 2021-06-03 2022-12-22 Illumina Software, Inc. Systems and methods for sequencing nucleotides using two optical channels
CN118056130A (en) 2021-07-01 2024-05-17 因美纳有限公司 Device with horizontal nanochannels for nanopore sequencing
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
KR20240049214A (en) 2021-09-01 2024-04-16 일루미나, 인코포레이티드 Amplitude modulation for accelerated base calling
WO2023049682A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Sequencing polynucleotides using nanopores
US12455277B2 (en) 2021-09-30 2025-10-28 Illumina, Inc. Isolation of cells in a nanopore sensor array
WO2023107622A1 (en) 2021-12-10 2023-06-15 Illumina, Inc. Parallel sample and index sequencing
EP4493721A1 (en) 2022-03-15 2025-01-22 Illumina, Inc. Parallel sample and index sequencing
US20230357307A1 (en) 2022-05-04 2023-11-09 Illumina, Inc. Cleavable cyclic loop nucleotides for nanopore sequencing
WO2025061942A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Sequencing error identification and correction
WO2025061922A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Methods for sequencing
WO2025064405A1 (en) 2023-09-22 2025-03-27 Illumina, Inc. Macromolecule blocks for nanopore sequencing
WO2025072861A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Cleavable cyclic loop nucleotides for nanopore sequencing
WO2025159980A1 (en) 2024-01-23 2025-07-31 Illumina, Inc. Ratchet-chelators as unique barcodes for nanopore sequencing
WO2025184183A1 (en) 2024-03-01 2025-09-04 Illumina, Inc. Designer peptide cyclic loops for nanopore sequencing
WO2025183909A1 (en) 2024-03-01 2025-09-04 Illumina, Inc. Ribose-mediated cyclic loop opening for nanopore sequencing
WO2025190902A1 (en) 2024-03-13 2025-09-18 Illumina, Inc. Improving base calling quality scores
WO2025245014A1 (en) 2024-05-24 2025-11-27 Illumina, Inc. Nuclease assisted nanopore sequencing

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6358727B1 (en) * 1993-11-08 2002-03-19 Aventis Pasteur Limited Haemophilus transferrin receptor genes
DK0655506T3 (en) 1994-10-17 1997-03-10 Harvard College DNA polymerase with modified nucleotide binding site for DNA sequence analysis
CZ293215B6 (en) 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Enzyme of thermally stable DNA polymerase, process of its preparation and a pharmaceutical composition and a kit containing thereof
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
EP2208789B1 (en) * 1997-03-12 2015-07-22 Applied Biosystems, LLC DNA polymerases having improved labeled nucleotide incorporation properties
JPH10248583A (en) 1997-03-17 1998-09-22 B M L:Kk DNA polymerase and DNA polymerase gene encoding the same
DE69842183D1 (en) 1997-08-29 2011-04-28 Life Technologies Corp HIGHLY SENSITIVE POLYMERASES AND ITS USES

Also Published As

Publication number Publication date
JP2003519488A (en) 2003-06-24
WO2001051621A3 (en) 2002-01-24
WO2001051621A2 (en) 2001-07-19
EP1246924A2 (en) 2002-10-09
US6602695B2 (en) 2003-08-05
US6329178B1 (en) 2001-12-11
US20020132249A1 (en) 2002-09-19

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