ATE550438T1 - Verfahren zur quantitativen bestimmung der methylierungsdichte in einem dna-locus - Google Patents
Verfahren zur quantitativen bestimmung der methylierungsdichte in einem dna-locusInfo
- Publication number
- ATE550438T1 ATE550438T1 AT04796212T AT04796212T ATE550438T1 AT E550438 T1 ATE550438 T1 AT E550438T1 AT 04796212 T AT04796212 T AT 04796212T AT 04796212 T AT04796212 T AT 04796212T AT E550438 T1 ATE550438 T1 AT E550438T1
- Authority
- AT
- Austria
- Prior art keywords
- methylation density
- quantitatively determining
- dna locus
- locus
- dna
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 230000011987 methylation Effects 0.000 title 1
- 238000007069 methylation reaction Methods 0.000 title 1
- 230000007067 DNA methylation Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51342603P | 2003-10-21 | 2003-10-21 | |
| US56172104P | 2004-04-12 | 2004-04-12 | |
| US56156304P | 2004-04-12 | 2004-04-12 | |
| PCT/US2004/035177 WO2005040399A2 (en) | 2003-10-21 | 2004-10-21 | Methods for quantitative determination of methylation density in a dna locus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE550438T1 true ATE550438T1 (de) | 2012-04-15 |
Family
ID=34527938
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04796072T ATE553218T1 (de) | 2003-10-21 | 2004-10-21 | Differentielle enzymatische fragmetierung |
| AT04796212T ATE550438T1 (de) | 2003-10-21 | 2004-10-21 | Verfahren zur quantitativen bestimmung der methylierungsdichte in einem dna-locus |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04796072T ATE553218T1 (de) | 2003-10-21 | 2004-10-21 | Differentielle enzymatische fragmetierung |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US7901880B2 (de) |
| EP (4) | EP1689887B1 (de) |
| JP (2) | JP4917891B2 (de) |
| AT (2) | ATE553218T1 (de) |
| AU (2) | AU2004286235B2 (de) |
| CA (2) | CA2542542C (de) |
| ES (2) | ES2383970T3 (de) |
| WO (2) | WO2005040399A2 (de) |
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| WO2003023065A1 (en) | 2001-09-06 | 2003-03-20 | Syngenta Participations Ag | Dna methylation patterns |
| US20050227230A1 (en) | 2001-09-14 | 2005-10-13 | Davies Collison Cave | Detection of dna methylation |
| JP2006500901A (ja) | 2001-09-26 | 2006-01-12 | エピジェンクス ファーマスーティカルズ、 インコーポレイテッド | Ｄｎａメチル化変化のアッセイ |
| US20030099997A1 (en) * | 2001-10-24 | 2003-05-29 | Bestor Timothy H. | Method for gene identification based on differential DNA methylation |
| DE10154318A1 (de) | 2001-10-26 | 2003-05-15 | Epigenomics Ag | Verfahren zur Analyse genomischer Methylierungsmuster |
| AU2002359436A1 (en) | 2001-11-13 | 2003-06-23 | Rubicon Genomics Inc. | Dna amplification and sequencing using dna molecules generated by random fragmentation |
| US6960436B2 (en) | 2002-02-06 | 2005-11-01 | Epigenomics Ag | Quantitative methylation detection in DNA samples |
| US20040038254A1 (en) | 2002-03-08 | 2004-02-26 | Risa Peoples | Compositions and methods for detecting nucleic acid methylation |
| US20030175828A1 (en) | 2002-03-15 | 2003-09-18 | Lazar James G. | Signal amplification by Hybrid Capture |
| AU2003220291A1 (en) | 2002-03-15 | 2003-09-29 | Epigenomics Ag | Discovery and diagnostic methods using 5-methylcytosine dna glycosylase |
| EP2339025B1 (de) * | 2002-06-26 | 2013-10-09 | Cold Spring Harbor Laboratory | Verfahren zur Bestimmung des Methylierungsprofils |
| AU2003279101A1 (en) | 2002-10-02 | 2004-04-23 | Northwestern University | Methylation profile of cancer |
| EP1606417A2 (de) | 2003-03-07 | 2005-12-21 | Rubicon Genomics Inc. | In-vitro-dna-immortalisierung und gesamtgenomamplifikation unter verwendung von aus willkürlich fragmentierter dna erzeugten bibliotheken |
| CA2542542C (en) * | 2003-10-21 | 2015-09-15 | Orion Genomics Llc | Method of detecting a quantity of methylation at a locus |
| US7459274B2 (en) | 2004-03-02 | 2008-12-02 | Orion Genomics Llc | Differential enzymatic fragmentation by whole genome amplification |
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