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AU2001288379A1 - Nested methylation-specific polymerase chain reaction cancer detection method - Google Patents

Nested methylation-specific polymerase chain reaction cancer detection method

Info

Publication number
AU2001288379A1
AU2001288379A1 AU2001288379A AU8837901A AU2001288379A1 AU 2001288379 A1 AU2001288379 A1 AU 2001288379A1 AU 2001288379 A AU2001288379 A AU 2001288379A AU 8837901 A AU8837901 A AU 8837901A AU 2001288379 A1 AU2001288379 A1 AU 2001288379A1
Authority
AU
Australia
Prior art keywords
gene
chain reaction
polymerase chain
detecting
detection method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001288379A
Inventor
Steven A. Belinsky
William A. Palmisano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lovelace Respiratory Research Institute
Original Assignee
Lovelace Respiratory Research Institute
LOVELACE RESPIRATORY RES INST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22855593&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2001288379(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lovelace Respiratory Research Institute, LOVELACE RESPIRATORY RES INST filed Critical Lovelace Respiratory Research Institute
Publication of AU2001288379A1 publication Critical patent/AU2001288379A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A molecular marker-based method for monitoring and detecting cancer in humans. Aberrant methylation of gene promoters is a marker for cancer risk in humans. A two-stage, or "nested" polymerase chain reaction method is disclosed for detecting methylated DNA sequences at sufficiently high levels of sensitivity to permit cancer screening in biological fluid samples, such as sputum, obtained non-invasively. The method is for detecting the aberrant methylation of the p16 gene, O 6-methylguanine-DNA methyltransferase gene, Death-associated protein kinase gene, RAS-associated family 1 gene, or other gene promoters. The method offers a potentially powerful approach to population-based screening for the detection of lung and other cancers.
AU2001288379A 2000-08-25 2001-08-24 Nested methylation-specific polymerase chain reaction cancer detection method Abandoned AU2001288379A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22805700P 2000-08-25 2000-08-25
US60228057 2000-08-25
PCT/US2001/026452 WO2002018649A1 (en) 2000-08-25 2001-08-24 Nested methylation-specific polymerase chain reaction cancer detection method

Publications (1)

Publication Number Publication Date
AU2001288379A1 true AU2001288379A1 (en) 2002-03-13

Family

ID=22855593

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001288379A Abandoned AU2001288379A1 (en) 2000-08-25 2001-08-24 Nested methylation-specific polymerase chain reaction cancer detection method

Country Status (7)

Country Link
US (1) US7214485B2 (en)
EP (1) EP1352089B1 (en)
AT (1) ATE347619T1 (en)
AU (1) AU2001288379A1 (en)
CA (1) CA2421102A1 (en)
DE (1) DE60125065T2 (en)
WO (1) WO2002018649A1 (en)

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US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
CA2488382A1 (en) * 2002-06-05 2003-12-18 Case Western Reserve University Methods and compositions for detecting cancers
AU2003290223A1 (en) * 2002-12-02 2004-06-23 Solexa Limited Determination of methylation of nucleic acid sequences
AU2003221217A1 (en) * 2003-03-13 2004-09-30 Beijing Institute For Cancer Research The method of in vitro detecting aberrant dysplasia, and the artificial nucleotides being used
WO2005040399A2 (en) 2003-10-21 2005-05-06 Orion Genomics Llc Methods for quantitative determination of methylation density in a dna locus
WO2005042713A2 (en) * 2003-10-28 2005-05-12 The Johns Hopkins University Quantitative multiplex methylation-specific pcr
CA2496997A1 (en) * 2004-02-13 2005-08-13 Affymetrix, Inc. Analysis of methylation status using nucleic acid arrays
WO2006009870A2 (en) * 2004-06-17 2006-01-26 Epigenomics Ag Compositions and methods for preventing carry-over contamination in nucleic acid amplification reactions
EP3342877B1 (en) * 2004-09-30 2020-01-29 Epigenomics AG Method for providing dna fragments derived from an archived sample
PT1871912E (en) 2005-04-15 2012-05-25 Epigenomics Ag Method for determining dna methylation in blood or urine samples
WO2007008693A2 (en) * 2005-07-09 2007-01-18 Lovelace Respiratory Research Institute Gene methylation as a biomarker in sputum
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US20080003609A1 (en) * 2006-05-10 2008-01-03 The Cleveland Clinic Foundation Method of detecting bladder urothelial carcinoma
CN101153336B (en) * 2006-09-27 2011-09-07 香港中文大学 method and kit for detecting DNA methylation degree
US7599898B2 (en) * 2006-10-17 2009-10-06 International Business Machines Corporation Method and apparatus for improved regression modeling
EP2508623B1 (en) 2006-11-20 2015-08-12 The Johns Hopkins University DNA methylation markers and methods of use
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
PL2479289T3 (en) 2007-06-08 2016-10-31 Method for methylation analysis
WO2009047756A2 (en) * 2007-10-11 2009-04-16 Bioview Ltd. Methods and kits for diagnosing lung cancer
EP2340314B8 (en) 2008-10-22 2015-02-18 Illumina, Inc. Preservation of information related to genomic dna methylation
US8394584B2 (en) 2008-12-19 2013-03-12 The Board Of Trustees Of The University Of Illinois Detecting and sorting methylated DNA using a synthetic nanopore
EP2218793A1 (en) * 2009-02-13 2010-08-18 Alphagenics International SA Detection of MGMT methylation in tumors
US8748091B2 (en) * 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
US8980560B2 (en) 2010-04-21 2015-03-17 Kagoshima University Method for analyzing DNA methylation
CN104250663B (en) * 2013-06-27 2017-09-15 北京大学 The high-flux sequence detection method on methylated CpG island
CN116144755A (en) * 2022-12-30 2023-05-23 中国辐射防护研究院 Application of LncRNA as radon exposure early injury diagnostic molecular marker and screening method

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US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5965722A (en) 1991-05-21 1999-10-12 Isis Pharmaceuticals, Inc. Antisense inhibition of ras gene with chimeric and alternating oligonucleotides
ES2091976T3 (en) 1991-06-20 1996-11-16 Hoffmann La Roche PERFECTED METHODS FOR THE AMPLIFICATION OF NUCLEIC ACID.
JPH0690756A (en) 1992-09-10 1994-04-05 Fuso Yakuhin Kogyo Kk Two stage thermocycle pcr method
US5556773A (en) 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
US5565331A (en) 1993-11-12 1996-10-15 The Regents Of The University Of California Nucleic acids encoding neural axon outgrowth modulators
US5986076A (en) 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US5843649A (en) 1995-03-01 1998-12-01 University Of Colorado Method of identifying clonal cell samples using heteroduplex generators
US5693467A (en) 1995-05-19 1997-12-02 The American Type Culture Collection Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers
US5871917A (en) 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
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AU7829398A (en) * 1997-06-09 1998-12-30 University Of Southern California A cancer diagnostic method based upon dna methylation differences
US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
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US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
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US6709818B1 (en) * 1999-10-13 2004-03-23 The Johns Hopkins University School Of Medicine Methods of diagnosing and treating hepatic cell proliferative disorders

Also Published As

Publication number Publication date
DE60125065D1 (en) 2007-01-18
ATE347619T1 (en) 2006-12-15
EP1352089A4 (en) 2004-10-20
CA2421102A1 (en) 2002-03-07
DE60125065T2 (en) 2007-06-28
US20040038245A1 (en) 2004-02-26
EP1352089B1 (en) 2006-12-06
WO2002018649A1 (en) 2002-03-07
US7214485B2 (en) 2007-05-08
EP1352089A1 (en) 2003-10-15

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