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ATE395420T1 - Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen - Google Patents

Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen

Info

Publication number
ATE395420T1
ATE395420T1 AT01953004T AT01953004T ATE395420T1 AT E395420 T1 ATE395420 T1 AT E395420T1 AT 01953004 T AT01953004 T AT 01953004T AT 01953004 T AT01953004 T AT 01953004T AT E395420 T1 ATE395420 T1 AT E395420T1
Authority
AT
Austria
Prior art keywords
nucleic acid
chimeric nucleic
polymerases
acid polymerases
thermostable
Prior art date
Application number
AT01953004T
Other languages
English (en)
Inventor
Dirk Loeffert
Andreas Missel
Jie Kang
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE395420T1 publication Critical patent/ATE395420T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)
  • Saccharide Compounds (AREA)
AT01953004T 2000-02-17 2001-02-16 Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen ATE395420T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50615300A 2000-02-17 2000-02-17

Publications (1)

Publication Number Publication Date
ATE395420T1 true ATE395420T1 (de) 2008-05-15

Family

ID=24013413

Family Applications (2)

Application Number Title Priority Date Filing Date
AT08151710T ATE491031T1 (de) 2000-02-17 2001-02-16 Thermostabile chimäre nukleinsäurepolymerasen und ihre verwendungen
AT01953004T ATE395420T1 (de) 2000-02-17 2001-02-16 Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT08151710T ATE491031T1 (de) 2000-02-17 2001-02-16 Thermostabile chimäre nukleinsäurepolymerasen und ihre verwendungen

Country Status (5)

Country Link
US (2) US8637288B2 (de)
EP (2) EP1259624B1 (de)
AT (2) ATE491031T1 (de)
DE (2) DE60143632D1 (de)
WO (1) WO2001061015A2 (de)

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US7560260B2 (en) 2002-07-25 2009-07-14 Bio-Rad Laboratories, Inc. Compositions with polymerase activity
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US7704712B2 (en) 2003-03-25 2010-04-27 Stratagene California DNA polymerase fusions and uses thereof
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US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
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US8652782B2 (en) * 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
US9481912B2 (en) * 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US20140038174A1 (en) * 2011-04-26 2014-02-06 Longhorn Vaccines And Diagnostics, Llc Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples
WO2009135093A2 (en) 2008-04-30 2009-11-05 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2352818B1 (de) * 2008-11-03 2016-02-10 Kapa Biosystems, Inc. Chimäre dna-polymerasen
US8519125B2 (en) * 2009-05-11 2013-08-27 Biomatrica, Inc. Compositions and methods for biological sample storage
CA2806670A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
CA2806734A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
HK1217117A1 (zh) 2012-12-20 2016-12-23 生物马特里卡公司 用於使pcr试剂稳定化的制剂和方法
US10023856B2 (en) * 2013-09-25 2018-07-17 Thermo Fisher Scientific Baltics Uab Enzyme composition for DNA end repair, adenylation, phosphorylation
EP2986719B1 (de) * 2013-12-06 2020-07-29 Bio-Rad Laboratories, Inc. Fusionspolymerasen
WO2015191632A1 (en) 2014-06-10 2015-12-17 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
US10364420B2 (en) 2015-12-07 2019-07-30 Bio-Rad Laboratories, Inc Dimeric reverse transcriptase
WO2017100212A1 (en) 2015-12-08 2017-06-15 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
US20240200040A1 (en) * 2019-12-23 2024-06-20 Bgi Shenzhen Chimeric dna polymerase and application thereof
CN117050967B (zh) * 2023-08-21 2024-04-09 南京诺唯赞生物科技股份有限公司 一种改善二代测序文库gc均衡性的方法

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US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
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DE10049211A1 (de) 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus

Also Published As

Publication number Publication date
EP1259624A2 (de) 2002-11-27
US20140154748A1 (en) 2014-06-05
EP1925669B1 (de) 2010-12-08
DE60133992D1 (de) 2008-06-26
WO2001061015A3 (de) 2002-04-25
US8637288B2 (en) 2014-01-28
EP1925669A1 (de) 2008-05-28
ATE491031T1 (de) 2010-12-15
US20030138805A1 (en) 2003-07-24
EP1259624B1 (de) 2008-05-14
WO2001061015A2 (de) 2001-08-23
DE60143632D1 (de) 2011-01-20

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