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AR129748A1 - Métodos y composiciones para controlar el tamaño del meristemo para el mejoramiento de cultivos - Google Patents

Métodos y composiciones para controlar el tamaño del meristemo para el mejoramiento de cultivos

Info

Publication number
AR129748A1
AR129748A1 ARP230101666A ARP230101666A AR129748A1 AR 129748 A1 AR129748 A1 AR 129748A1 AR P230101666 A ARP230101666 A AR P230101666A AR P230101666 A ARP230101666 A AR P230101666A AR 129748 A1 AR129748 A1 AR 129748A1
Authority
AR
Argentina
Prior art keywords
plants
fea2
seq
corn plant
gene
Prior art date
Application number
ARP230101666A
Other languages
English (en)
Inventor
Devin Lee Oconnor
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of AR129748A1 publication Critical patent/AR129748A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Esta invención está relacionada con composiciones y métodos para modificar los genes FASCIATED EAR2 (FEA2) en plantas, opcionalmente para modificar el tamaño del meristemo. La invención además está relacionada con plantas que tienen un número aumentado de filas de granos producidas con el uso de los métodos y las composiciones de la invención. Reivindicación 1: Una planta de maíz o parte de planta de maíz que comprende al menos una mutación en un gen endógeno FASCIATED EAR2 (FEA2) que codifica un polipéptido FEA2. Reivindicación 2: La planta de maíz o parte de esta de la reivindicación 1, donde el gen endógeno FASCIATED EAR2 (FEA2) tiene el número de identificación de gen (ID de gen) Zm00001d051012. Reivindicación 11: La planta de maíz o parte de esta de cualquiera de las reivindicaciones precedentes, donde el gen endógeno FASCIATED EAR2 (FEA2) a) comprende una secuencia de nucleótidos que tiene al menos 90% de identidad de secuencia con SEQ ID Nº 72 o SEQ ID Nº 73, b) comprende una región que tiene al menos 90% de identidad de secuencia con cualquiera de las secuencias de nucleótidos de SEQ ID Nº 75 - 77, c) codifica una secuencia de aminoácidos que tiene al menos 90% de identidad de secuencia con SEQ ID Nº 74, y/o d) codifica un polipéptido que comprende una región que tiene al menos 90% de identidad de secuencia con cualquiera de las secuencias de aminoácidos de SEQ ID Nº 78 - 80. Reivindicación 15: La planta de maíz o parte de esta de cualquiera de las reivindicaciones precedentes, donde la al menos una mutación resulta en un gen FEA2 mutado que comprende una secuencia de nucleótidos que tiene al menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 82, 84, 86, 88, 90, 92, 94, 96 o 98 y/o codifica un polipéptido FEA2 mutado que tiene al menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 83, 85, 87, 89, 91, 93, 95, 97, o 99. Reivindicación 16: Una célula de planta de maíz que comprende un sistema de edición, el sistema de edición comprende: a) una proteína efectora CRISPR-Cas; y b) un ácido nucleico guía que comprende una secuencia espaciadora con complementariedad con un gen diana endógeno que codifica una proteína FEA2. Reivindicación 100: Un método para controlar malezas en un contenedor (por ejemplo, maceta, o almácigo y similares), una cámara de crecimiento, un invernadero, un campo, un área recreacional, un césped, o a la vera de un camino, que comprende aplicar un herbicida a una o más (una pluralidad) de plantas de cualquiera de las reivindicaciones 1 - 15, 19 - 21, 35, 36, 76, 93 o 94, que crecen en un contenedor, una cámara de crecimiento, un invernadero, un campo, un área recreacional, un césped, o a la vera de un camino, por lo cual se controlan las malezas en el contenedor, la cámara de crecimiento, el invernadero, el campo, el área recreacional, el césped, o en la vera del camino donde la única o más plantas están creciendo. Reivindicación 101: Un método para reducir la depredación de insectos en una planta de maíz, que comprende aplicar un insecticida a una o más plantas de cualquiera de las reivindicaciones 1 - 15, 19 - 21, 35, 36, 76, 93 o 94, por lo cual se reduce la depredación de insectos en la única o más plantas de maíz. Reivindicación 102: Un método para reducir la enfermedad fúngica en una planta de maíz, que comprende aplicar un fungicida a una o más plantas de cualquiera de las reivindicaciones 1 - 15, 19 - 21, 35, 36, 76, 93 o 94, por lo cual se reduce la enfermedad fúngica en la única o más plantas de maíz.
ARP230101666A 2022-06-29 2023-06-28 Métodos y composiciones para controlar el tamaño del meristemo para el mejoramiento de cultivos AR129748A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202263356527P 2022-06-29 2022-06-29

Publications (1)

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AR129748A1 true AR129748A1 (es) 2024-09-25

Family

ID=87517494

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Application Number Title Priority Date Filing Date
ARP230101666A AR129748A1 (es) 2022-06-29 2023-06-28 Métodos y composiciones para controlar el tamaño del meristemo para el mejoramiento de cultivos

Country Status (8)

Country Link
US (1) US20240002873A1 (es)
EP (1) EP4547693A1 (es)
CN (1) CN119365481A (es)
AR (1) AR129748A1 (es)
CA (1) CA3259679A1 (es)
MX (1) MX2024015770A (es)
UY (1) UY40337A (es)
WO (1) WO2024006792A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250122522A1 (en) * 2023-10-11 2025-04-17 Pairwise Plants Services, Inc. Methods and compositions for improving crop yield traits

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