AR111761A1 - Un método para purificar un anticuerpo o un fragmento de anticuerpo de este usando cromatografía de afinidad - Google Patents
Un método para purificar un anticuerpo o un fragmento de anticuerpo de este usando cromatografía de afinidadInfo
- Publication number
- AR111761A1 AR111761A1 ARP180101273A ARP180101273A AR111761A1 AR 111761 A1 AR111761 A1 AR 111761A1 AR P180101273 A ARP180101273 A AR P180101273A AR P180101273 A ARP180101273 A AR P180101273A AR 111761 A1 AR111761 A1 AR 111761A1
- Authority
- AR
- Argentina
- Prior art keywords
- antibody
- antibody fragment
- affinity chromatography
- purifying
- fragment
- Prior art date
Links
- 102000008394 Immunoglobulin Fragments Human genes 0.000 title abstract 5
- 108010021625 Immunoglobulin Fragments Proteins 0.000 title abstract 5
- 238000001042 affinity chromatography Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 239000012149 elution buffer Substances 0.000 abstract 2
- 239000011347 resin Substances 0.000 abstract 1
- 229920005989 resin Polymers 0.000 abstract 1
- 239000011534 wash buffer Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
Abstract
En forma específica, a un método para separar el anticuerpo y fragmento de anticuerpo de este con alta pureza y purificar el mismo usando un buffer de elución capaz de aumentar la estabilidad para la mejora de la estabilidad. Reivindicación 1: Un método para purificar un anticuerpo blanco o un fragmento de anticuerpo de este, que comprende: (a) cargar una muestra que comprende un anticuerpo o un fragmento de anticuerpo de este en una columna de cromatografía de afinidad que comprende una resina de afinidad de cadena pesada; (b) lavar la columna con un buffer de lavado; y (c) recuperar el anticuerpo blanco o el fragmento de anticuerpo de este de la columna de cromatografía de afinidad usando un buffer de elución, que tiene un pH que varía de pH 3,8 a pH 4,7.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20170060535 | 2017-05-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR111761A1 true AR111761A1 (es) | 2019-08-14 |
Family
ID=64603003
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP180101273A AR111761A1 (es) | 2017-05-16 | 2018-05-15 | Un método para purificar un anticuerpo o un fragmento de anticuerpo de este usando cromatografía de afinidad |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR102087823B1 (es) |
| AR (1) | AR111761A1 (es) |
| TW (1) | TWI679052B (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102209790B1 (ko) * | 2018-12-20 | 2021-02-01 | 에이치케이이노엔 주식회사 | 친화성 크로마토그래피를 이용한 백신용 바이러스 정제방법 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2009282234A1 (en) * | 2008-08-14 | 2010-02-18 | Merck Sharp & Dohme Corp. | Methods for purifying antibodies using protein a affinity chromatography |
| AR096713A1 (es) * | 2013-06-25 | 2016-01-27 | Cadila Healthcare Ltd | Proceso de purificación para anticuerpos monoclonales |
| WO2016075034A1 (en) * | 2014-11-10 | 2016-05-19 | F. Hoffmann-La Roche Ag | ANTI-IL-1beta ANTIBODIES AND METHODS OF USE |
-
2018
- 2018-05-15 KR KR1020180055313A patent/KR102087823B1/ko not_active Expired - Fee Related
- 2018-05-15 TW TW107116454A patent/TWI679052B/zh not_active IP Right Cessation
- 2018-05-15 AR ARP180101273A patent/AR111761A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| TW201900257A (zh) | 2019-01-01 |
| KR20180125899A (ko) | 2018-11-26 |
| TWI679052B (zh) | 2019-12-11 |
| KR102087823B1 (ko) | 2020-03-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |