AR117418A1 - Métodos para la purificación de polipéptidos usando polisorbatos - Google Patents
Métodos para la purificación de polipéptidos usando polisorbatosInfo
- Publication number
- AR117418A1 AR117418A1 ARP190101058A ARP190101058A AR117418A1 AR 117418 A1 AR117418 A1 AR 117418A1 AR P190101058 A ARP190101058 A AR P190101058A AR P190101058 A ARP190101058 A AR P190101058A AR 117418 A1 AR117418 A1 AR 117418A1
- Authority
- AR
- Argentina
- Prior art keywords
- protein
- chromatography material
- applying
- polysorbate
- impurity
- Prior art date
Links
- 229920000136 polysorbate Polymers 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 229920001184 polypeptide Polymers 0.000 title 1
- 229940068965 polysorbates Drugs 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 238000004587 chromatography analysis Methods 0.000 abstract 8
- 239000000463 material Substances 0.000 abstract 8
- 102000004169 proteins and genes Human genes 0.000 abstract 8
- 108090000623 proteins and genes Proteins 0.000 abstract 8
- 230000003301 hydrolyzing effect Effects 0.000 abstract 4
- 239000012535 impurity Substances 0.000 abstract 4
- 229950008882 polysorbate Drugs 0.000 abstract 4
- 239000000203 mixture Substances 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Oncology (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
En este documento se presenta un método para purificar una proteína de una muestra que comprende la proteína y al menos una impureza con actividad hidrolítica, que comprende las siguientes etapas: i) a) aplicar la muestra que comprende la proteína y al menos una impureza con actividad hidrolítica a un material de cromatografía, b) aplicar una solución que comprende un polisorbato al material de cromatografía, y c) recuperar la proteína del material de cromatografía, o ii) a) añadir un polisorbato a la muestra que comprende la proteína y al menos una impureza con actividad hidrolítica, b) aplicar la mezcla de la etapa a) a un material de cromatografía, y c) recuperar la proteína del material de cromatografía, o iii) a) añadir un polisorbato a la muestra que comprende la proteína y al menos una impureza con actividad hidrolítica, b) aplicar la mezcla de la etapa a) a un material de cromatografía, c) aplicar una solución que comprende un polisorbato al material de cromatografía, y d) recuperar la proteína del material de cromatografía.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP18169762.4A EP3560945A1 (en) | 2018-04-27 | 2018-04-27 | Methods for purification of polypeptides using polysorbates |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR117418A1 true AR117418A1 (es) | 2021-08-04 |
Family
ID=62167103
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP190101058A AR117418A1 (es) | 2018-04-27 | 2019-04-23 | Métodos para la purificación de polipéptidos usando polisorbatos |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20210155656A1 (es) |
| EP (2) | EP3560945A1 (es) |
| JP (3) | JP2021520399A (es) |
| KR (1) | KR102613595B1 (es) |
| CN (1) | CN112041327A (es) |
| AR (1) | AR117418A1 (es) |
| AU (1) | AU2019258509A1 (es) |
| BR (1) | BR112020021897A2 (es) |
| CA (1) | CA3096562A1 (es) |
| IL (1) | IL278220A (es) |
| MX (1) | MX2020010886A (es) |
| SG (1) | SG11202010618PA (es) |
| TW (1) | TW201945056A (es) |
| WO (1) | WO2019207021A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MY204234A (en) | 2018-08-27 | 2024-08-16 | Regeneron Pharma | Use of raman spectroscopy in downstream purification |
| TW202138556A (zh) * | 2019-12-20 | 2021-10-16 | 美商默沙東藥廠 | 經純化之腸病毒組合物及使用穀胱甘肽親和性層析之純化方法 |
| CN116033921A (zh) * | 2020-08-07 | 2023-04-28 | 豪夫迈·罗氏有限公司 | 用于生产蛋白质组合物的方法 |
| CA3222181A1 (en) * | 2021-06-14 | 2022-12-22 | Regeneron Pharmaceuticals, Inc. | Monitoring polysorbate hydrolysis in pharmaceutical formulations using an ultrasensitive extraction-free fatty acid quantitation method |
| AU2022429920A1 (en) * | 2021-12-31 | 2024-07-25 | Kashiv Biosciences, Llc | A process for separation and quantification of non-ionic surfactant |
| WO2024096505A1 (ko) * | 2022-10-31 | 2024-05-10 | 삼성바이오에피스 주식회사 | 친화성 크로마토그래피를 통해 불순물을 제거하는 방법 |
| WO2024107474A1 (en) * | 2022-11-15 | 2024-05-23 | Massachusetts Institute Of Technology | Controlled lipid self-assembly for scalable manufacturing of next-generation immune stimulating complexes |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0940468A1 (en) | 1991-06-14 | 1999-09-08 | Genentech, Inc. | Humanized antibody variable domain |
| WO1993021319A1 (en) | 1992-04-08 | 1993-10-28 | Cetus Oncology Corporation | HUMANIZED C-erbB-2 SPECIFIC ANTIBODIES |
| JPH08504172A (ja) | 1992-06-30 | 1996-05-07 | オンコロジクス，インコーポレイティド | 抗−ｅｒｂＢ−２モノクロナール抗体の組み合わせ物及び使用方法 |
| US5783186A (en) | 1995-12-05 | 1998-07-21 | Amgen Inc. | Antibody-induced apoptosis |
| EP1308455B9 (en) * | 1998-05-06 | 2006-06-14 | Genentech, Inc. | A composition comprising anti-HER2 antibodies |
| KR100850389B1 (ko) | 1999-06-25 | 2008-08-04 | 제넨테크, 인크. | 인간화 항-ＥｒｂＢ2 항체 및 항-ＥｒｂＢ2 항체를 사용한치료 방법 |
| CA2473144C (en) * | 2002-02-05 | 2013-05-28 | Genentech, Inc. | Protein purification |
| BRPI0416262B1 (pt) | 2003-11-05 | 2022-04-12 | Roche Glycart Ag | Anticorpo anti-cd20 humano tipo ii humanizado, seu método de produção, seus usos, bem como polinucleotídeo isolado, vetor de expressão e composição farmacêutica |
| BRPI0510883B8 (pt) | 2004-06-01 | 2021-05-25 | Genentech Inc | composto conjugado de droga e anticorpo, composição farmacêutica, método de fabricação de composto conjugado de droga e anticorpo e usos de uma formulação, de um conjugado de droga e anticorpo e um agente quimioterapêutico e de uma combinação |
| JO3000B1 (ar) | 2004-10-20 | 2016-09-05 | Genentech Inc | مركبات أجسام مضادة . |
| WO2007109163A2 (en) * | 2006-03-16 | 2007-09-27 | Amgen Inc | Wash buffer and method of using |
| US9345661B2 (en) | 2009-07-31 | 2016-05-24 | Genentech, Inc. | Subcutaneous anti-HER2 antibody formulations and uses thereof |
| BR112012030530A2 (pt) * | 2010-05-18 | 2022-12-20 | Abbvie Inc | Aparelho e processo para purificação de proteínas |
| MX361337B (es) | 2012-07-13 | 2018-12-04 | Roche Glycart Ag | Anticuerpos biespecificos anti-factor de crecimiento endotelial vascular humano (vegf) / anti-angiopoyetina-2 humana (ang-2) y su uso en el tratamiento de enfermedades vasculares oculares. |
| US8946395B1 (en) * | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| WO2015095568A1 (en) | 2013-12-18 | 2015-06-25 | Kelvin Lee | Reduction of lipase activity in product formulations |
| TW201628649A (zh) * | 2014-10-09 | 2016-08-16 | 再生元醫藥公司 | 減少醫藥調配物中微可見顆粒之方法 |
| GB2542391A (en) * | 2015-09-17 | 2017-03-22 | Annexin Pharmaceuticals Ab | Process of manufacture |
| BR112018010945A2 (pt) | 2015-12-30 | 2018-12-04 | Genentech Inc | formulações com degradação de polissorbato reduzida |
| AU2017316955B2 (en) * | 2016-08-25 | 2022-03-03 | Jcr Pharmaceuticals Co., Ltd. | Method for producing antibody fusion protein |
-
2018
- 2018-04-27 EP EP18169762.4A patent/EP3560945A1/en not_active Withdrawn
-
2019
- 2019-04-23 AR ARP190101058A patent/AR117418A1/es unknown
- 2019-04-25 WO PCT/EP2019/060557 patent/WO2019207021A1/en not_active Ceased
- 2019-04-25 JP JP2020560138A patent/JP2021520399A/ja not_active Withdrawn
- 2019-04-25 MX MX2020010886A patent/MX2020010886A/es unknown
- 2019-04-25 EP EP19720113.0A patent/EP3784683A1/en active Pending
- 2019-04-25 AU AU2019258509A patent/AU2019258509A1/en active Pending
- 2019-04-25 KR KR1020207032494A patent/KR102613595B1/ko active Active
- 2019-04-25 CA CA3096562A patent/CA3096562A1/en active Pending
- 2019-04-25 CN CN201980028690.2A patent/CN112041327A/zh active Pending
- 2019-04-25 SG SG11202010618PA patent/SG11202010618PA/en unknown
- 2019-04-25 BR BR112020021897-9A patent/BR112020021897A2/pt unknown
- 2019-04-26 TW TW108114799A patent/TW201945056A/zh unknown
-
2020
- 2020-10-21 IL IL278220A patent/IL278220A/en unknown
- 2020-10-26 US US17/080,505 patent/US20210155656A1/en active Pending
-
2022
- 2022-12-23 JP JP2022206521A patent/JP2023030126A/ja active Pending
-
2025
- 2025-02-20 JP JP2025025494A patent/JP2025081536A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3560945A1 (en) | 2019-10-30 |
| CA3096562A1 (en) | 2019-10-31 |
| MX2020010886A (es) | 2020-11-09 |
| EP3784683A1 (en) | 2021-03-03 |
| JP2021520399A (ja) | 2021-08-19 |
| TW201945056A (zh) | 2019-12-01 |
| BR112020021897A2 (pt) | 2021-03-09 |
| WO2019207021A1 (en) | 2019-10-31 |
| KR102613595B1 (ko) | 2023-12-13 |
| CN112041327A (zh) | 2020-12-04 |
| IL278220A (en) | 2020-11-30 |
| AU2019258509A1 (en) | 2020-11-19 |
| JP2023030126A (ja) | 2023-03-07 |
| JP2025081536A (ja) | 2025-05-27 |
| US20210155656A1 (en) | 2021-05-27 |
| KR20200142551A (ko) | 2020-12-22 |
| SG11202010618PA (en) | 2020-11-27 |
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