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MXPA06000266A - Ensayos electrofisiologicos mejorados utilizando oocitos que expresan un enac humano y el uso de fenamilo para mejorar el efecto de intensificadores del enac en ensayos. - Google Patents

Ensayos electrofisiologicos mejorados utilizando oocitos que expresan un enac humano y el uso de fenamilo para mejorar el efecto de intensificadores del enac en ensayos.

Info

Publication number
MXPA06000266A
MXPA06000266A MXPA06000266A MXPA06000266A MXPA06000266A MX PA06000266 A MXPA06000266 A MX PA06000266A MX PA06000266 A MXPA06000266 A MX PA06000266A MX PA06000266 A MXPA06000266 A MX PA06000266A MX PA06000266 A MXPA06000266 A MX PA06000266A
Authority
MX
Mexico
Prior art keywords
enac
assays
human
compounds
phenamil
Prior art date
Application number
MXPA06000266A
Other languages
English (en)
Inventor
Imran Clark
Original Assignee
Senomyx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Senomyx Inc filed Critical Senomyx Inc
Publication of MXPA06000266A publication Critical patent/MXPA06000266A/es

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    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

En un aspecto, la presente invencion se refiere a un ensayo de alto rendimiento basado en celulas de mamifero para el perfilado y seleccion de un canal de sodio epitelial humano (hENaC) clonado de una biblioteca de ADNc de rinon humano y tambien es expresado en otros tejidos que incluyen el tejido gustativo humano. La presente invencion se refiere ademas a ensayos electrofisiologicos de rendimiento intermedio basados en oocitos de anfibio para identificar moduladores del ENaC humano, preferiblemente intensificadores del ENaC. Se espera que los compuestos que modulan la funcion del ENaC en un ensayo de ENaC basado en celulas afecten el sabor salado en humanos. Los ensayos descritos en este documento tienen ventajas sobre los sistemas de expresion celulares existentes. En el caso de celulas de mamifero, estos ensayos pueden conducirse en placas de cultivo de 96 a 384 pocillos estandar en un modo de alto rendimiento con resultados de ensayo mejorados que son logrados mediante el uso de un compuesto que inhibe la funcion del ENaC, preferiblemente un derivado de amilorida tal como fenamilo. En el caso de los ensayos electrofisiologicos de oocitos inventivos (tecnica de fijacion de voltaje de dos electrodos), estos ensayos facilitan la identificacion de compuestos que modulan especificamente un ENaC humano. Los ensayos de la invencion proporcionan una seleccion robusta que es util para detectar compuestos que facilitan (intensifican) o inhiben la funcion del ENaC. Los compuestos que intensifican o bloquean la actividad del canal ENaC humano deben modular con lo cual el sabor salado en humanos.
MXPA06000266A 2003-07-10 2004-07-09 Ensayos electrofisiologicos mejorados utilizando oocitos que expresan un enac humano y el uso de fenamilo para mejorar el efecto de intensificadores del enac en ensayos. MXPA06000266A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48574503P 2003-07-10 2003-07-10
PCT/US2004/021853 WO2005014848A2 (en) 2003-07-10 2004-07-09 IMPROVED ELECTROPHYSIOLOGICAL ASSAYS USING OOCYTES THAT EXPRESS HUMAN ENaC AND THE USE OF PHENAMIL TO IMPROVE THE EFFECT OF ENaC ENHANCERS IN ASSAYS USING MEMBRANE POTENTIAL REPORTING DYES

Publications (1)

Publication Number Publication Date
MXPA06000266A true MXPA06000266A (es) 2006-03-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA06000266A MXPA06000266A (es) 2003-07-10 2004-07-09 Ensayos electrofisiologicos mejorados utilizando oocitos que expresan un enac humano y el uso de fenamilo para mejorar el efecto de intensificadores del enac en ensayos.

Country Status (12)

Country Link
US (5) US9459259B2 (es)
EP (1) EP1644738A2 (es)
JP (1) JP2007528712A (es)
KR (1) KR20060029680A (es)
CN (1) CN1842709A (es)
AU (1) AU2004263845A1 (es)
BR (1) BRPI0412471A (es)
CA (1) CA2530497A1 (es)
IL (1) IL172933A0 (es)
MX (1) MXPA06000266A (es)
NO (1) NO20060078L (es)
WO (1) WO2005014848A2 (es)

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CA2530497A1 (en) * 2003-07-10 2005-02-17 Senomyx, Inc. Improved electrophysiological assays using oocytes that express human enac and the use of phenamil to improve the effect of enac enhancers in assays using membrane potential reporting dyes
CA2666962C (en) * 2006-10-19 2015-12-08 Monell Chemical Senses Center Human salty taste receptor and methods of modulating salty taste perception
EP2245057B1 (en) 2008-01-25 2019-09-04 Chromocell Corporation Novel cell lines expressing enac and methods using them
CA2713885A1 (en) * 2008-02-01 2009-08-20 Chromocell Corporation Novel cell lines and methods
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JP2012191850A (ja) * 2009-07-24 2012-10-11 Ajinomoto Co Inc インドール誘導体及び同誘導体を含有する塩味増強剤
JP5857378B2 (ja) * 2010-04-23 2016-02-10 アローヘッド リサーチ コーポレイション ベータ−ＥＮａＣ−関連疾患を処置するための有機組成物
EP2622970A4 (en) * 2010-09-29 2014-04-16 Ajinomoto Kk SALT TASTE AMPLIFIERS
JP6039891B2 (ja) * 2011-07-14 2016-12-07 花王株式会社 上皮型ナトリウムチャネル活性化剤
CN104964909B (zh) * 2015-05-28 2018-04-24 上海市血液中心 一种血小板跨膜电位检测方法
GB201719169D0 (en) * 2017-11-20 2018-01-03 Univ College Cardiff Consultants Ltd Novel T-cell receptor and ligand
CN109164158A (zh) * 2018-07-17 2019-01-08 中国科学院南京土壤研究所 电生理试验中记录微小电流的方法
CN109364248B (zh) * 2018-10-16 2021-05-18 哈尔滨医科大学 ENaC及其抑制剂在预防、缓解和/或治疗动脉粥样硬化中的应用
JP2022059100A (ja) * 2019-02-26 2022-04-13 味の素株式会社 ナトリウムの作用を調節する物質のスクリーニング方法
CN110530829A (zh) * 2019-08-20 2019-12-03 北京爱思益普生物科技股份有限公司 一种基于荧光的早期药物筛选方法

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Also Published As

Publication number Publication date
WO2005014848A2 (en) 2005-02-17
US20170175160A1 (en) 2017-06-22
CA2530497A1 (en) 2005-02-17
US20120070857A1 (en) 2012-03-22
NO20060078L (no) 2006-04-07
KR20060029680A (ko) 2006-04-06
US20090123942A1 (en) 2009-05-14
EP1644738A2 (en) 2006-04-12
US8105792B2 (en) 2012-01-31
US20050059094A1 (en) 2005-03-17
US20160069899A9 (en) 2016-03-10
JP2007528712A (ja) 2007-10-18
US10048274B2 (en) 2018-08-14
AU2004263845A1 (en) 2005-02-17
US20190242905A1 (en) 2019-08-08
IL172933A0 (en) 2006-06-11
BRPI0412471A (pt) 2006-09-19
US10215759B2 (en) 2019-02-26
CN1842709A (zh) 2006-10-04
WO2005014848A3 (en) 2005-05-06
US9459259B2 (en) 2016-10-04

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