MX2021003995A - Plasmido que contiene una secuencia que codifica un arnm con una cola de poli(a) segmentada. - Google Patents
Plasmido que contiene una secuencia que codifica un arnm con una cola de poli(a) segmentada.Info
- Publication number
- MX2021003995A MX2021003995A MX2021003995A MX2021003995A MX2021003995A MX 2021003995 A MX2021003995 A MX 2021003995A MX 2021003995 A MX2021003995 A MX 2021003995A MX 2021003995 A MX2021003995 A MX 2021003995A MX 2021003995 A MX2021003995 A MX 2021003995A
- Authority
- MX
- Mexico
- Prior art keywords
- tail
- elements
- nucleotide
- nucleotides
- mrna
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
- A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/50—Vectors for producing vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente divulgación proporciona un plásmido de ADN que comprende una secuencia que codifica una molécula de ARNm y una cola de poli(A) modificada, en el que la parte de la secuencia que codifica la cola de poli(A) modificada se caracteriza porque ésta consiste de al menos dos elementos A cada uno definido como una secuencia de nucleótidos que consiste en de 55 a 65 nucleótidos T y al menos un elemento S cada elemento S consiste en un nucleótido que no es un nucleótido T, o 2 a 10 nucleótidos, preferiblemente 6 nucleótidos, en el que cada uno de los dos nucleótidos terminales no es un nucleótido T, en el que el número de elementos A es uno más que el número de elementos S, y en el que dos elementos A cualesquiera están separados por un elemento S; exhibiendo dicho plásmido de ADN una recombinación reducida durante la amplificación en una célula huésped bacteriana en comparación con el mismo plásmido de ADN sin dicho al menos un elemento S.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP18199857 | 2018-10-11 | ||
| PCT/EP2019/077477 WO2020074642A1 (en) | 2018-10-11 | 2019-10-10 | Plasmid containing a sequence encoding an mrna with a segmented poly(a) tail |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2021003995A true MX2021003995A (es) | 2021-06-23 |
Family
ID=64023934
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2021003995A MX2021003995A (es) | 2018-10-11 | 2019-10-10 | Plasmido que contiene una secuencia que codifica un arnm con una cola de poli(a) segmentada. |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US20210348172A1 (es) |
| EP (2) | EP3864156B1 (es) |
| JP (1) | JP7573520B2 (es) |
| KR (1) | KR102772300B1 (es) |
| CN (1) | CN112805386B (es) |
| AU (1) | AU2019358238B2 (es) |
| BR (1) | BR112021006597A2 (es) |
| CA (1) | CA3115597A1 (es) |
| DK (1) | DK3864156T5 (es) |
| ES (1) | ES2958832T3 (es) |
| HU (1) | HUE063060T2 (es) |
| MX (1) | MX2021003995A (es) |
| PL (1) | PL3864156T3 (es) |
| PT (1) | PT3864156T (es) |
| WO (1) | WO2020074642A1 (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2926020T3 (es) | 2014-12-12 | 2022-10-21 | Curevac Ag | Moléculas de ácido nucleico artificiales para una expresión proteica mejorada |
| US20240344019A1 (en) * | 2021-08-17 | 2024-10-17 | Sk Bioscience Co., Ltd. | Method for stably maintaining poly(a) tail |
| TW202325850A (zh) | 2021-11-29 | 2023-07-01 | 大陸商上海瑞宏迪醫藥有限公司 | Aadc、gdnf多核苷酸及其用於治療帕金森病 |
| TW202342526A (zh) | 2022-02-21 | 2023-11-01 | 大陸商上海瑞宏迪醫藥有限公司 | Ｖｅｇｆ結合分子及其醫藥用途 |
| WO2024121160A1 (en) | 2022-12-05 | 2024-06-13 | Ethris Gmbh | Regulator(s) of energy homeostasis-encoding rna molecule(s) with increased translation efficiency |
| KR102852704B1 (ko) * | 2022-12-26 | 2025-09-01 | 주식회사 엔이에스바이오테크놀러지 | 안정화를 위한 3'UTR 서열을 포함하는 mRNA 구조체 |
| WO2024179544A1 (zh) * | 2023-03-01 | 2024-09-06 | 仁景(苏州)生物科技有限公司 | 编码rna的工程化dna分子 |
| WO2024184626A2 (en) | 2023-03-03 | 2024-09-12 | Diosynvax Ltd | Coronavirus vaccines |
| AU2024248317A1 (en) * | 2023-03-29 | 2025-11-13 | Shenzhen Shenxin Biotechnology Co., Ltd. | Polynucleotide molecule comprising poly(a) and use thereof |
| WO2024240962A1 (en) | 2023-05-25 | 2024-11-28 | Ethris Gmbh | Gm-csf-encoding nucleic acids, pharmaceutical compositions, methods and uses thereof |
| WO2025017151A1 (en) | 2023-07-18 | 2025-01-23 | Sanofi | Stable poly(a)-encoding messenger rna templates |
| WO2025036956A1 (en) | 2023-08-14 | 2025-02-20 | Ethris Gmbh | Non-systemic mrna administration |
| CN120665913A (zh) * | 2024-03-18 | 2025-09-19 | 康希诺(上海)生物研发有限公司 | 一种mRNA体外表达质粒、构建方法及其应用 |
| GB202406051D0 (en) | 2024-04-30 | 2024-06-12 | Univ Oxford Innovation Ltd | Segmented poly(a) tails |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002508299A (ja) * | 1997-09-19 | 2002-03-19 | セクイター， インク． | センスｍＲＮＡ治療 |
| US20040031072A1 (en) | 1999-05-06 | 2004-02-12 | La Rosa Thomas J. | Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement |
| US20110131679A2 (en) | 2000-04-19 | 2011-06-02 | Thomas La Rosa | Rice Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement |
| EP3165234B1 (de) | 2009-07-31 | 2019-04-03 | ethris GmbH | Rna mit einer kombination aus unmodifizierten und modifizierten nucleotiden zur proteinexpression |
| US20110059453A1 (en) | 2009-08-23 | 2011-03-10 | Affymetrix, Inc. | Poly(A) Tail Length Measurement by PCR |
| WO2016005004A1 (en) | 2014-07-11 | 2016-01-14 | Biontech Rna Pharmaceuticals Gmbh | Stabilization of poly(a) sequence encoding dna sequences |
| ES2667644T3 (es) * | 2015-06-30 | 2018-05-14 | Ethris Gmbh | UTR que aumentan la eficacia de la traducción de las moléculas de ARN |
| CN105505755A (zh) * | 2015-12-23 | 2016-04-20 | 杭州谷禾信息技术有限公司 | 空间转录组建库测序方法及所用装置 |
| CN105734053B (zh) * | 2016-04-20 | 2018-12-14 | 武汉生命之美科技有限公司 | 一种高通量测序分析Poly(A)尾长度文库的构建方法 |
-
2019
- 2019-10-10 CA CA3115597A patent/CA3115597A1/en active Pending
- 2019-10-10 EP EP19782636.5A patent/EP3864156B1/en active Active
- 2019-10-10 BR BR112021006597-0A patent/BR112021006597A2/pt unknown
- 2019-10-10 WO PCT/EP2019/077477 patent/WO2020074642A1/en not_active Ceased
- 2019-10-10 US US17/284,779 patent/US20210348172A1/en active Pending
- 2019-10-10 ES ES19782636T patent/ES2958832T3/es active Active
- 2019-10-10 JP JP2021519784A patent/JP7573520B2/ja active Active
- 2019-10-10 HU HUE19782636A patent/HUE063060T2/hu unknown
- 2019-10-10 EP EP23191351.8A patent/EP4306643A3/en active Pending
- 2019-10-10 AU AU2019358238A patent/AU2019358238B2/en active Active
- 2019-10-10 PT PT197826365T patent/PT3864156T/pt unknown
- 2019-10-10 DK DK19782636.5T patent/DK3864156T5/da active
- 2019-10-10 PL PL19782636.5T patent/PL3864156T3/pl unknown
- 2019-10-10 MX MX2021003995A patent/MX2021003995A/es unknown
- 2019-10-10 CN CN201980066650.7A patent/CN112805386B/zh active Active
- 2019-10-10 KR KR1020217012107A patent/KR102772300B1/ko active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20210348172A1 (en) | 2021-11-11 |
| KR102772300B1 (ko) | 2025-02-26 |
| JP2022504663A (ja) | 2022-01-13 |
| BR112021006597A2 (pt) | 2021-07-20 |
| CA3115597A1 (en) | 2020-04-16 |
| ES2958832T3 (es) | 2024-02-15 |
| CN112805386A (zh) | 2021-05-14 |
| HUE063060T2 (hu) | 2023-12-28 |
| CN112805386B (zh) | 2024-12-03 |
| EP3864156A1 (en) | 2021-08-18 |
| PL3864156T3 (pl) | 2024-01-03 |
| EP3864156B1 (en) | 2023-08-16 |
| EP4306643A2 (en) | 2024-01-17 |
| JP7573520B2 (ja) | 2024-10-25 |
| AU2019358238B2 (en) | 2025-05-22 |
| WO2020074642A1 (en) | 2020-04-16 |
| KR20210075102A (ko) | 2021-06-22 |
| EP4306643A3 (en) | 2024-03-20 |
| PT3864156T (pt) | 2023-10-04 |
| AU2019358238A1 (en) | 2021-05-06 |
| DK3864156T5 (da) | 2024-08-12 |
| DK3864156T3 (da) | 2023-10-09 |
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