[go: up one dir, main page]

MX2020013357A - Polimerasas, composiciones y metodos de uso. - Google Patents

Polimerasas, composiciones y metodos de uso.

Info

Publication number
MX2020013357A
MX2020013357A MX2020013357A MX2020013357A MX2020013357A MX 2020013357 A MX2020013357 A MX 2020013357A MX 2020013357 A MX2020013357 A MX 2020013357A MX 2020013357 A MX2020013357 A MX 2020013357A MX 2020013357 A MX2020013357 A MX 2020013357A
Authority
MX
Mexico
Prior art keywords
polymerases
compositions
methods
incorporation
altered
Prior art date
Application number
MX2020013357A
Other languages
English (en)
Inventor
Misha Golynskiy
Seth Mcdonald
Saurabh Nirantar
Sergio Peisajovich
Kay Klausing
Hamed Tabatabaei Ghomi
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2020013357A publication Critical patent/MX2020013357A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se presentan en la presente enzimas polimerasa alteradas para la incorporación mejorada de nucleótidos y análogos de nucleótidos, en particular polimerasas alteradas que mantienen alta fidelidad bajo tiempos de incorporación reducidos, así como método y kits que utilizan las mismas.
MX2020013357A 2018-12-05 2019-12-04 Polimerasas, composiciones y metodos de uso. MX2020013357A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862775662P 2018-12-05 2018-12-05
PCT/US2019/064524 WO2020117968A2 (en) 2018-12-05 2019-12-04 Polymerases, compositions, and methods of use

Publications (1)

Publication Number Publication Date
MX2020013357A true MX2020013357A (es) 2021-04-28

Family

ID=69137980

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020013357A MX2020013357A (es) 2018-12-05 2019-12-04 Polimerasas, composiciones y metodos de uso.

Country Status (14)

Country Link
US (4) US11001816B2 (es)
EP (1) EP3891275A2 (es)
JP (1) JP7527208B2 (es)
KR (1) KR20210098844A (es)
CN (1) CN112639090A (es)
AU (1) AU2019391093B2 (es)
BR (1) BR112020026655A2 (es)
CA (1) CA3103739A1 (es)
IL (2) IL297994A (es)
MX (1) MX2020013357A (es)
MY (1) MY204767A (es)
SG (1) SG11202012559SA (es)
WO (1) WO2020117968A2 (es)
ZA (1) ZA202007839B (es)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112673098B (zh) 2018-10-31 2023-01-06 亿明达股份有限公司 聚合酶、组合物及使用方法
IL297994A (en) 2018-12-05 2025-01-01 Illumina Inc Polymerases, compounds, and methods of use
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
WO2021104845A1 (en) 2019-11-27 2021-06-03 Illumina Cambridge Limited Cyclooctatetraene containing dyes and compositions
CA3165571C (en) 2019-12-23 2023-02-07 Singular Genomics Systems, Inc. Methods for long read sequencing
EP4085135A4 (en) 2019-12-31 2023-08-02 Singular Genomics Systems, Inc. Polynucleotide barcodes for long read sequencing
US11174281B1 (en) 2020-04-24 2021-11-16 Singular Genomics Systems, Inc. Modified nucleotides and uses thereof
BR112022026056A2 (pt) 2020-06-22 2023-03-07 Illumina Cambridge Ltd Nucleosídeos e nucleotídeos com grupo bloqueador 3? acetal
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
US12054506B2 (en) 2021-03-19 2024-08-06 Singular Genomics Systems, Inc. Reducing agents and uses thereof
EP4334327A1 (en) 2021-05-05 2024-03-13 Illumina Cambridge Limited Fluorescent dyes containing bis-boron fused heterocycles and uses in sequencing
US12270056B2 (en) 2021-06-18 2025-04-08 Element Biosciences, Inc. Engineered polymerases
US12077789B2 (en) * 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CA3222937A1 (en) 2021-12-29 2023-07-06 Jonathan Mark Boutell Methods of nucleic acid sequencing using surface-bound primers
US12163163B2 (en) 2022-02-18 2024-12-10 Element Biosciences, Inc. Engineered polymerases with reduced sequence-specific errors
WO2023186815A1 (en) 2022-03-28 2023-10-05 Illumina Cambridge Limited Labeled avidin and methods for sequencing
US20230314322A1 (en) 2022-03-29 2023-10-05 Illumina, Inc. Systems and methods of sequencing polynucleotides
WO2023186872A1 (en) 2022-03-30 2023-10-05 Illumina Cambridge Limited Methods for chemical cleavage of surface-bound polynucleotides
AU2023244351A1 (en) 2022-03-31 2024-01-18 Illumina, Inc. Nucleosides and nucleotides with 3' vinyl blocking group useful in sequencing by synthesis
WO2023186982A1 (en) 2022-03-31 2023-10-05 Illumina, Inc. Compositions and methods for improving sequencing signals
US20230383342A1 (en) 2022-05-31 2023-11-30 Illumina Cambridge Limited Compositions and methods for nucleic acid sequencing
CN119604513A (zh) 2022-06-28 2025-03-11 因美纳公司 含有稠合四环双硼杂环的荧光染料及其在测序中的用途
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
AU2023348238A1 (en) 2022-09-30 2024-10-03 Illumina, Inc. Compositions and methods for reducing photo damage during sequencing
US20240240217A1 (en) 2022-12-09 2024-07-18 Illumina, Inc. Nucleosides and nucleotides with 3' blocking groups and cleavable linkers
US20240247311A1 (en) 2022-12-22 2024-07-25 Illumina, Inc. Palladium catalyst compositions and methods for sequencing by synthesis
CA3246536A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. TRANSITION METAL CATALYST COMPOSITIONS AND SYNTHETIC SEQUENCE PROCESSES
AU2023419253A1 (en) 2022-12-27 2024-10-03 Illumina, Inc. Methods of sequencing using 3´ allyl blocked nucleotides
US20240327910A1 (en) 2023-03-29 2024-10-03 Illumina, Inc. Naphthalimide dyes and uses in nucleic acid sequencing
CN116286720A (zh) * 2023-03-29 2023-06-23 赛纳生物科技(北京)有限公司 一种dna聚合酶
CA3257470A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. NUCLEIC ACID COMPOSITIONS AND SEQUENCE METHODS
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
US20240401129A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Methods for double-stranded sequencing by synthesis
WO2024249061A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Detecting a methylated nucleotide using a quencher
US20250075269A1 (en) 2023-08-31 2025-03-06 Illumina, Inc. Compositions and methods for nucleic acid sequencing
US20250137048A1 (en) 2023-10-26 2025-05-01 Illumina, Inc. 4,5-substituted naphthalimide dyes and uses in nucleic acid sequencing
WO2025106715A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Conjugated polymers or polymer dots as fluorescent labels
US20250197933A1 (en) 2023-12-18 2025-06-19 Illumina, Inc. Hydrogel nanoparticles as labeling scaffold in sequencing
US20250215493A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
US20250215491A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Sequencing with single-stranded binding proteins
US20250215494A1 (en) 2023-12-29 2025-07-03 Illumina, Inc. Tricyclic polymethine dyes for nucleic acid sequencing
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025230914A1 (en) 2024-04-29 2025-11-06 Illumina, Inc. Nucleotides with enzyme-triggered self-immolative linkers for sequencing by synthesis

Family Cites Families (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA258147A (en) 1926-02-16 W. Pease John Apple paring machine
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
CA2251643A1 (en) 1996-04-15 1997-10-23 University Of Alberta Synthesis of fluorophore-labeled dna
DE69725076T2 (de) 1996-07-29 2004-04-15 Toyo Boseki K.K. Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US5882904A (en) 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
US5948666A (en) 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
US20050244870A1 (en) 1999-04-20 2005-11-03 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
DE60024464T2 (de) 1999-05-07 2006-08-03 Roche Diagnostics Gmbh Hohe dichte markierung von dns mit modifizierte oder chromophore tragende nukleotide und benutzte dns polymerasen
US6492161B1 (en) 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
JP2003510063A (ja) 1999-09-30 2003-03-18 ニュー・イングランド・バイオラブズ・インコーポレイティッド 古細菌のｄｎａポリメラーゼによる修飾ヌクレオチドの取り込み及びそれに関する方法
US20030228616A1 (en) 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
AU1239901A (en) 1999-10-29 2001-05-14 Stratagene Compositions and methods utilizing dna polymerases
US8268605B2 (en) 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
EP1232270A1 (en) 1999-11-23 2002-08-21 Amersham Biosciences Corp. IMPROVING DIDEOXYNUCLEOTIDE-TRIPHOSPHATE UTILIZATION BY THE HYPER-THERMOPHILIC DNA POLYMERASE FROM THE ARCHAEON i PYROCOCCUS FURIOSUS /i
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
AU2007201277B2 (en) 2000-05-24 2011-10-13 Third Wave Technologies, Inc. Detection of RNA
EP1368460B1 (en) 2000-07-07 2007-10-31 Visigen Biotechnologies, Inc. Real-time sequence determination
WO2002010358A2 (en) 2000-07-31 2002-02-07 Maxygen, Inc. Nucleotide incorporating enzymes
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
CA2450139A1 (en) 2001-06-11 2002-12-19 Illumina, Inc Multiplexed detection methods
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
AU2005296200B2 (en) 2004-09-17 2011-07-14 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US20070048748A1 (en) 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
GB0509508D0 (en) * 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
JP2009521227A (ja) 2005-12-22 2009-06-04 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド ヌクレオチドアナログ組込み用ポリメラーゼ
JP5122555B2 (ja) 2006-03-31 2013-01-16 ソレクサ・インコーポレイテッド 合成解析による配列決定システムおよび装置
DK2044188T3 (en) 2006-07-14 2015-12-14 Novozymes As Polypeptides with lipase activity and polynucleotides encoding them
GB0617575D0 (en) 2006-09-06 2006-10-18 Syngenta Ltd Herbicidal compounds and compositions
ES2404665T3 (es) 2006-09-06 2013-05-28 Medical Research Council Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante
EP2089517A4 (en) 2006-10-23 2010-10-20 Pacific Biosciences California POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING
EP3285067B1 (en) 2006-12-14 2022-06-22 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
WO2008083393A2 (en) 2006-12-29 2008-07-10 Stratagene California Compositions and methods using split polymerases
US7892797B2 (en) 2007-12-27 2011-02-22 Ge Healthcare Bio-Sciences Corp. Single enzyme system for fast, ultra long PCR
US20110045489A1 (en) 2008-04-22 2011-02-24 New England Biolabs, Inc. Polymerases for Incorporating Modified Nucleotides
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010062779A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Modified dna polymerases
WO2010062776A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
WO2010082640A1 (ja) 2009-01-15 2010-07-22 北海道三井化学株式会社 耐熱性dnaポリメラーゼを含む酵素調製物およびその製造方法、並びに検出対象生物の検出方法
WO2011026194A1 (en) 2009-09-07 2011-03-10 Reproductive Health Science Pty Ltd Nucleic acid extraction
US8965076B2 (en) 2010-01-13 2015-02-24 Illumina, Inc. Data processing system and methods
US20110301041A1 (en) 2010-02-26 2011-12-08 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
GB201007384D0 (en) 2010-04-30 2010-06-16 Medical Res Council Enzymes
EP2632593B1 (en) 2010-10-27 2021-09-29 Illumina, Inc. Flow cells for biological or chemical analysis
WO2012154934A1 (en) 2011-05-11 2012-11-15 New England Biolabs, Inc. Dna polymerase variants with reduced exonuclease activity and uses thereof
US8921044B2 (en) 2011-05-11 2014-12-30 New England Biolabs, Inc. DNA polymerase variants with reduced exonuclease activity and uses thereof
JP6478445B2 (ja) 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
WO2014142921A1 (en) * 2013-03-14 2014-09-18 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
EP2970356B1 (en) 2013-03-15 2018-05-30 Illumina Cambridge Limited Modified nucleosides or nucleotides
GB2526261B (en) 2014-04-28 2017-08-02 Gelliner Ltd Encoded cells and cell arrays
AU2015279794B2 (en) 2014-06-27 2021-09-30 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
WO2016033315A2 (en) * 2014-08-27 2016-03-03 New England Biolabs, Inc. Synthon formation
US9677057B2 (en) 2014-09-30 2017-06-13 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
EP4006150A1 (en) 2015-09-09 2022-06-01 QIAGEN GmbH Polymerase enzyme
US10662413B2 (en) 2016-11-01 2020-05-26 Personal Genomics, Inc. Recombinant DNA polymerase for improved incorporation of nucleotide analogues
EP3613851A4 (en) 2017-01-09 2020-07-01 MGI Tech Co., Ltd. RECOMBINANT DNA POLYMERASE
WO2018148723A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus abyssi
WO2018148724A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
WO2018148727A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from 9°n
WO2018148726A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
EP3580351A1 (en) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymerase enzyme from 9°n
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
CN112639089B (zh) 2018-09-03 2024-02-09 深圳华大生命科学研究院 重组型kod聚合酶
US20230011240A1 (en) 2018-09-11 2023-01-12 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
US10731141B2 (en) 2018-09-17 2020-08-04 Omniome, Inc. Engineered polymerases for improved sequencing
CN112673098B (zh) 2018-10-31 2023-01-06 亿明达股份有限公司 聚合酶、组合物及使用方法
IL297994A (en) 2018-12-05 2025-01-01 Illumina Inc Polymerases, compounds, and methods of use
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group

Also Published As

Publication number Publication date
CN112639090A (zh) 2021-04-09
IL279423A (en) 2021-01-31
EP3891275A2 (en) 2021-10-13
SG11202012559SA (en) 2021-01-28
IL279423B (en) 2022-08-01
MY204767A (en) 2024-09-12
US11001816B2 (en) 2021-05-11
IL297994A (en) 2025-01-01
ZA202007839B (en) 2022-04-28
CA3103739A1 (en) 2020-06-11
KR20210098844A (ko) 2021-08-11
JP7527208B2 (ja) 2024-08-02
US11634697B2 (en) 2023-04-25
WO2020117968A3 (en) 2020-08-13
US20250059521A1 (en) 2025-02-20
US20210348141A1 (en) 2021-11-11
US20230357731A1 (en) 2023-11-09
US20200181587A1 (en) 2020-06-11
AU2019391093B2 (en) 2025-11-27
WO2020117968A2 (en) 2020-06-11
US12104182B2 (en) 2024-10-01
JP2022511206A (ja) 2022-01-31
AU2019391093A1 (en) 2021-01-07
BR112020026655A2 (pt) 2021-04-06

Similar Documents

Publication Publication Date Title
MX2020013357A (es) Polimerasas, composiciones y metodos de uso.
MX2020013347A (es) Polimerasas, composiciones y metodos de uso.
MX2022004101A (es) Composiciones de oligonucleotidos y metodos de uso de las mismas.
CO2019003846A2 (es) Tratamiento aav de la enfermedad de huntington
MX2020010974A (es) Composiciones y métodos para la edición de genes.
ECSP18010463A (es) Composiciones de oligonucleótidos y métodos de los mismos
BR112017007770A2 (pt) cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.
BR112018074979A2 (pt) composições de limpeza que compreendem enzimas
MX2017016688A (es) Nuevas enzimas y sistemas crispr.
CR20200192A (es) Métodos y composiciones para la producción de nucleósido trifosfatos y ácidos ribonucleicos
BR112017012765A2 (pt) ?métodos para editar uma sequência de nucleotídeos, célula e linhagem de e. coli e método para produzir uma célula de e. coli?
EP4328303A3 (en) Novel use
UY37360A (es) Nucleósidos sustituidos, nucleótidos y análogos de estos
ES2721204T3 (es) Circularización y amplificación de ácidos nucleicos circulantes asistida por ligasa
UY34701A (es) Formas sólidas de un profármaco de nucleótidos de tiofosforamidato
CL2016003277A1 (es) Métodos para preparar análogos de nucleótido sustituidos
MX2019013370A (es) Constructos de anticuerpos de ácidos nucleicos optimizados.
EP4461821A3 (en) Polymerase compositions and methods of making and using same
MX2024000277A (es) Microarns modificados con 5-halouracilo y su uso en el tratamiento del cancer.
CL2022002779A1 (es) Compuestos y métodos para modular el proceso de corte y empalme
PH12020550654A1 (en) Asymmetric pcr methods
MX2023008629A (es) Nuevas nucleasas modificadas y quimericas.
MX2019007894A (es) Composiciones y metodos para detectar el adn tumoral circulante.
ZA202101476B (en) Methods and compositions for nucleic acid isolation
CL2017002608A1 (es) Agentes nucleósidos para la reducción de la actividad perjudicial de los genes que contienen repeticiones de nucleótidos extendidas.